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Fig. 2 in Detection of Breinlia sp. (Nematoda) in the Leadbeater's possum (Gymnobelideus leadbeateri)

Fig. 2. Relationship of the novel Breinlia sp. Taxon (bold-type) from the lung of the Leadbeater's possum with members of the Onchocercidae, established by phylogenetic analysis of small subunit of nuclear ribosomal RNA gene (SSU) sequence data employing the Bayesian method. Posterior probabilities are indicated at nodes. Mastophorus muris was used as an outgroup.

Fig. 7. Stained microfilariae from amphibian blood. A – Neofoleyellides steyni n in Two new species of Neofoleyellides (Nematoda: Onchocercidae) parasitising anuran amphibians in South Africa

Fig. 7. Stained microfilariae from amphibian blood. A – Neofoleyellides steyni n. sp. from Amietia delalandii (Dum´eril et Bibron, 1841); B – Neofoleyellides martini n. sp. from Leptopelis natalensis (Smith, 1849).

Fig. 4. Neofoleyellides martini n in Two new species of Neofoleyellides (Nematoda: Onchocercidae) parasitising anuran amphibians in South Africa

Fig. 4. Neofoleyellides martini n. sp. from Leptopelis natalensis (Smith, 1849), line drawings. A – fragment of body at anterior end, female, lateral view; B – fragment of body at anterior end, male, lateral view; C – anterior extremity, female, lateral view; D–F – anterior extremity, female, apical view, optical sections at different depth of focus; G – posterior end of body, male, ventral view; H – microfilaria; I – posterior end of body, female, lateral view; J – spicules, lateral view.

Fig. 8 in Two new species of Neofoleyellides (Nematoda: Onchocercidae) parasitising anuran amphibians in South Africa

Fig. 8. Phylogeny of selected amphibian and reptilian filarial nematodes from the family Onchocercidae. Phylogram based on partitioned and concatenated datasets of 18S rDNA, and COI mtDNA sequences using Maximum Likelihood. Filaria latala (GenBank Accession numbers – 18S: KP760135 and COI: KP760186] was chosen as the outgroup. The total length of datasets is 1293 nucleotides, containing 11 taxa. The scale bar represents 0.09 nucleotide substitutions per site.

Fig. 6. Neofoleyellides martini n in Two new species of Neofoleyellides (Nematoda: Onchocercidae) parasitising anuran amphibians in South Africa

Fig. 6. Neofoleyellides martini n. sp. from Leptopelis natalensis (Smith, 1849), photomicrographs. A – transverse section at posterior end of body, male, a – ala; B – area rugosa.

Fig. 1. Neofoleyellides steyni n in Two new species of Neofoleyellides (Nematoda: Onchocercidae) parasitising anuran amphibians in South Africa

Fig. 1. Neofoleyellides steyni n. sp. from Amietia delalandii (Dum´eril et Bibron, 1841), line drawings. A – fragment of body at anterior end, female, lateral view; B – fragment of body at anterior end, male, lateral view; C – anterior extremity, female, lateral view; D–G – anterior extremity, female, apical view, optical sections at different depth of focus; H – microfilaria; I – posterior end of body, female, lateral view.

Fig. 5. Neofoleyellides martini n in Two new species of Neofoleyellides (Nematoda: Onchocercidae) parasitising anuran amphibians in South Africa

Fig. 5. Neofoleyellides martini n. sp. from Leptopelis natalensis (Smith, 1849), line drawings. A–D – posterior end of body, male, ventral view, variations of the arrangements of caudal papillae.

Fig. 3. Neofoleyellides steyni n in Two new species of Neofoleyellides (Nematoda: Onchocercidae) parasitising anuran amphibians in South Africa

Fig. 3. Neofoleyellides steyni n. sp. from Amietia delalandii (Dumeril´et Bibron, 1841), photomicrographs. A–C – lateral alae, male: A – anterior end, B – midbody level, C – transverse section at level of posterior end, la – left ala, ra – right ala; D – area rugosa.

Fig. 2. Neofoleyellides steyni n in Two new species of Neofoleyellides (Nematoda: Onchocercidae) parasitising anuran amphibians in South Africa

Fig. 2. Neofoleyellides steyni n. sp. from Amietia delalandii (Dum´eril et Bibron, 1841), line drawings. A – posterior end of body, male, lateral view; B – right spicule, lateral view; C – distal end of the left spicule, lateral view; D–I – posterior end of body, male, ventral view, variations of the arrangements of caudal papillae.

Figure 5 in Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

Figure 5: Bayesian 50% majority rule consensus tree inferred from D2-D3 expansion segments of large subunit (LSU) rDNA gene sequence of CephalenChUS dRiekieae n. sp. from South Africa under GTR + I + G model (lnL = 7,632.2964; freqA = 0.1794; freqC = 0.2463; freqG = 0.3573; freqT = 0.2170; rAC = 0.9583; rAG = 2.6626; rAT = 1.2424; rCG = 0.6711; rCT = 5.4981; Pinv = 0.2528; Alpha = 0.6707). Bayesian posterior probability (BPP) values>0.50 are given for appropriate clades. The sequence of the new species is indicated by bold font.

Figure 4 in Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

Figure 4: Bayesian 50% majority rule consensus tree inferred from small subunit (SSU) rDNA gene sequence of CephalenChUS dRiekieae n. sp. from South Africa under GTR + I + G model (lnL = 6,436.7202; freqA = 0.2443; freqC = 0.2268; freqG = 0.2864; freqT = 0.2425; rAC = 1.0699; rAG = 2.8891; rAT = 1.2180; rCG = 1.1273; rCT = 5.8691; Pinv = 0.4073; Alpha = 0.6044). Bayesian posterior probability (BPP) values>0.50 are given for appropriate clades. The sequence of the new species is indicated by bold font.

Figure 2 in Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

Figure 2: Light micrographs of CephalenChUS dRiekieae n. sp. from South Africa, female. (A) Entire body; (B) pharyngeal region; (C) lateral lines; (D) part of reproduction system; (E) anterior region; (F) pharyngeal overlap; (G) tail; (H) tail terminus. (Scale bars = 10 µm).

Figure 3 in Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

Figure 3: Scanning electron microscopy of CephalenChUS dRiekieae n. sp. from South Africa, female. (A) Anterior region; (B) lip region; (C) posterior body region; (D, E) vulval region in lateral and ventral view; (F) anus and lateral lines in anus region.

Figure 1 in Cephalenchus driekieae n. sp. (Nematoda: Tylenchidae) from South Africa, a new member of the genus with a long pharyngeal overlap

Figure 1: Line drawings of CephalenChUS dRiekieae n. sp. from South Africa, female. (A) Entire body; (B) anterior region; (C) pharyngeal region; (D) reproductive system; (E) tail.

Figure 7 in A new cyst-forming nematode, Cactodera tianzhuensis n. sp. (Nematoda:Heteroderinae) from Polygonum viviparum in China with a key to the Genus Cactodera

Figure 7: Molecular phylogenetic tree of C. tianZhUenSiS n. sp. (highlighted in bold) inferred from ITS region under GTR + I + G model. The posterior probability values exceeding 50% are given on appropriate clades. *Originally identified as C. eStOniCa in the GenBank. **Originally identified as C. eremiCa in the GenBank. ***Originally identified as C. eStOniCa in the GenBank. ****Originally identified as C. eStOniCa in the GenBank.

Figure 6 in A new cyst-forming nematode, Cactodera tianzhuensis n. sp. (Nematoda:Heteroderinae) from Polygonum viviparum in China with a key to the Genus Cactodera

Figure 6: Molecular phylogenetic tree of C. tianZhUenSiS n. sp. (highlighted in bold) inferred from 28 S D2/D3 extension region under GTR + I + G model. The posterior probability values exceeding 50% are given on appropriate clades. *Originally identified as C. eStOniCa in the GenBank. **Originally identified as C. rOSae in the GenBank. ***Originally identified as C. eStOniCa in the GenBank.

Figure 4 in A new cyst-forming nematode, Cactodera tianzhuensis n. sp. (Nematoda:Heteroderinae) from Polygonum viviparum in China with a key to the Genus Cactodera

Figure 4: Egg of C. tianZhUenSiS n. sp. A: Smooth eggshell; B: Embryo egg; C: Body of developed J2 in egg. (Scale bar = 20 µm).

Figure 3 in A new cyst-forming nematode, Cactodera tianzhuensis n. sp. (Nematoda:Heteroderinae) from Polygonum viviparum in China with a key to the Genus Cactodera

Figure 3: Vulval cones of C. tianZhUenSiS n. sp. A, C: Fenestration in vulval cone (inside); B, D: Fenestration in vulval cone (outside); E, F: Cyst surface punctations. (Scale bar = 20 µm).

Figure 2 in A new cyst-forming nematode, Cactodera tianzhuensis n. sp. (Nematoda:Heteroderinae) from Polygonum viviparum in China with a key to the Genus Cactodera

Figure 2: Light micrographs of C. tianZhUenSiS n. sp. A: Cyst; B: Cysts; C: Crush cyst; C: Female attached on the root (Scale bar: A, D = 500 μm; B, C = 1 mm).

Figure 1 in A new cyst-forming nematode, Cactodera tianzhuensis n. sp. (Nematoda:Heteroderinae) from Polygonum viviparum in China with a key to the Genus Cactodera

Figure 1: Line drawing of C. tianZhUenSiS n. sp. A: Anterior region of second-stage juvenile; B: Head of second-stage juvenile; C, H: Cyst and cysts. D: Lateral field; E: Fenestration in vulval cone; F, G: Tail of second-stage juvenile (Scale bar: H = 500 μm; C = 200 μm; A, F, E = 20 μm; B = 10 μm; D, G = 5 μm).