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Fig. 3 in Taxonomic and Morphogenetic Description of the Freshwater Ciliate Aponotohymena isoaustralis n. sp. (Ciliophora; Oxytrichidae) Isolated from Sanjay Lake, Delhi, India

Fig. 3. Line diagrams showing morphogenetic stages on ventral surface of protargol impregnated cells of Aponotohymena isoaustralis n. sp. A, B – origin of OP apokinetally between the LMC and POVC for the opisthe; C – reorganization of parental UM (arrow), disaggregation of II/2 (arrowhead), III/2 (double arrowhead) and V/4 (double arrow) to form primordia IIp, IIIp and Vo respectively, kinetosomes from OP form primordia Io and IIo; D – dissagregation of IV/3 to form primordium IVp (arrow); kinetosomes from OP move anteriorly (arrowhead); the two primary primordia, one each formed from disaggregation of V/4 and V/3 split transversely (double arrow) to form primordia V and VI for proter and opisthe; E – full complement of 6 FVT primordia Ip to VIp (arrowhead) and Io to VIo (double arrowhead); F – within-row marginal primordia formation for RMC (arrowheads) and LMC (double arrowheads); G – differentiation of cirri in 1, 3, 3, 3, 4, 4 pattern; H – late divider showing formation of new dorsomarginals (arrowheads) close to newly formed RMC. LMC – left marginal cirri; OP – oral primordium. Scale bar: 20 µm.

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Fig. 19 in Microsporidia in a Woodland Pool I. Lanatospora costata sp. n. (Opisthosporidia, Microsporidia), Parasite of Megacyclops viridis (Crustacea, Copepoda): Fine Structure and Molecular Phylogeny

Fig. 19. The woodland pool near Přerov nad Labem, Central Bohemia Region, Czech Republic (50°167′N, 14°810′E), the type habitat of Lanatospora costata sp. n.

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Fig. 1 in Trichodinids (Ciliophora) of Corydoras paleatus (Siluriformes) and Jenynsia multidentata (Cyprinodontiformes) from Argentina, with Description of Trichodina corydori n. sp. and Trichodina jenynsii n. sp.

Fig. 1. Photomicrographs of Trichodina corydori n. sp. from Corydoras paleatus. A–B – Silver nitrate-impregnated adhesive discs. C – Methylene-blue staining of adoral ciliary spiral. D – and orcein stain of macronucleus. Scale bars: 10 μm.

Fig. 2 in Taxonomic and Morphogenetic Description of the Freshwater Ciliate Aponotohymena isoaustralis n. sp. (Ciliophora; Oxytrichidae) Isolated from Sanjay Lake, Delhi, India

Fig. 2. Line diagrams showing protargol impregnated vegetative cells of Aponotohymena isoaustralis n. sp. A – ventral surface; B – dorsal surface. AZM – adoral zone of membranelles, CC – caudal cirri, DK1–4 – dorsal kineties, DM1, 2 – dorsomarginals, EM – endoral membrane, LMC – left marginal cirri, PM – paroral membrane, RMC – right marginal cirri, II/2 – buccal cirri, I/1, II/3, III/3 – frontal cirri, VI/4, VI/3, IV/3, III/2 – frontoventral cirri, – IV/2, V/4, V/3 – postoral ventral cirri, V/2 and VI/2 – pretransverse ventral cirri, II/1, III/1, IV/1, V/1, VI/1 – transverse cirri. Scale bar: 20 µm.

Fig. 1 in Taxonomic and Morphogenetic Description of the Freshwater Ciliate Aponotohymena isoaustralis n. sp. (Ciliophora; Oxytrichidae) Isolated from Sanjay Lake, Delhi, India

Fig. 1. Photomicrographs of live (A, B, E, F, G, H, J, K), protargol impregnated (C, D, I, L, M) and Feulgen stained (N) cells of Aponotohymena isoaustralis n. sp. A, B – cells in ventral view; C – ventral view of a vegetative cell with 5 transverse cirri arranged in a pseudo row (arrow); D – dorsal view of a vegetative cell; E – ventral view to show the arrangement of cortical granules (arrowheads) and colou- ration; F, G and H – ventral view of different cells showing flexible body; I – anterior portion of the dorsal surface showing dorsal rows (arrowhead); J – anterior portion of the cell showing contractile vacuole (arrowhead); K – cyst; L – anterior hook (arrowhead) of paroral membrane; M – dorsal view showing caudal cirri (2 + 2 + 3) (arrowheads); N – two macronuclei. AZM – adoral zone of membranelles, LMC – left marginal cirri, RMC – right marginal cirri. Scale bars: 20 µm.

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Fig. 1. Glugea sardinellensis n in New Microsporidia, Glugea sardinellensis n. sp. (Microsporea, Glugeida) Found in Sardinella aurita Valenciennes, 1847, Collected off Tunisian coasts

Fig. 1. Glugea sardinellensis n. sp. infecting Sardinella aurita. (A–B) Infected round sardinella showing different sized xenomas in the pyloric caeca (arrows) (scale bar: 1 cm). (C) Fresh spores of G. sardinellensis n. sp. (scale bar: 5 µm). (D–E) Semi-thin section of the pe- ripheral region of the xemona showing the wall (XW) and numerous spores (scale bar: 20 µm). Cf – collagen fibers; Fb – fibroblast cells.

Fig. 6 in Description of a New Brackish Water Ciliate, Uronychia xinjiangensis n. sp. (Ciliophora, Euplotida) Based on Morphology, Morphogenesis and Molecular Phylogeny

Fig. 6. Photomicrographs of Uronychia xinjiangensis n. sp. after protargol staining (A–I). (A–C) Opisthe's oral primordium at early dividers. (D) Proter's oral primordium. (E, F) Fontal-ventral-transverse cirral anlagen of early dividers. (G) A later divider showing the completion of development of oral primordium and cirral anlagen, arrows and arrow show newly formed ventral and frontal cirri respectively in the opisthe. (H, I) The same late divider showing the posterior part of adoral zone of membranelles (arrowheads) and the longest dorsal kinety 3 (arrows). AZM1, the anterior part of adoral zone of membranelles; CA, cirral anlagen; CC, caudal cirri; LMC, left marginal cirri; OP, opisthe's oral primordium; POP, proter's oral primordium; TC, transverse cirri. Scale bars: 20 μm.

Fig. 7 in Description of a New Brackish Water Ciliate, Uronychia xinjiangensis n. sp. (Ciliophora, Euplotida) Based on Morphology, Morphogenesis and Molecular Phylogeny

Fig. 7. Phylogenetic tree inferred by ML and BI of SSU rRNA gene sequences. Numbers near branches denote ML bootstraps value/BI posterior probability value. '*' indicates topology that differ between ML and BI phylogenies. All branches are drawn to scale. The scale bar corresponds to 5 substitutions per 100 nucleotide positions. GenBank accession numbers are given for each species. Classification is mainly according to Lynn (2008).

Fig. 4 in Description of a New Brackish Water Ciliate, Uronychia xinjiangensis n. sp. (Ciliophora, Euplotida) Based on Morphology, Morphogenesis and Molecular Phylogeny

Fig. 4. Microphotographs of Uronychia xinjiangensis n. sp. from life (A–L). (A, C, D) Ventral views of different cells, showing variation of body shape, arrow shows cilia in AZM1. (B) Dorsal view showing inclusions, a small spur-like protrusion (arrow in B), and anterior membranelles (arrow in D). (E) Partially lateral view. (F) Anterior portion showing spur-like bulge (arrow) at anterior margin of body and AZM1. (G) Ventral view of posterior part showing AZM2, and small left marginal cirrus (arrow). (H) Ventral view of anterior part, showing frontal cirri. (I) Ventral view of posterior part, showing the fine rightmost transverse cirrus (arrow) and left marginal cirri. (J) Ventral view, showing two fine ventral cirri (arrows). (K) Depicting dorsal bristles. (L) Dorsal view, to show caudal cirri located at concave area and dorsal grooves (arrows). AZM1,2, anterior and proximal part of adoral zone of membranelles; CC, caudal cirri; FC, frontal cirri; LMC, left marginal cirri; TC, transverse cirri. Scale bars: 20 μm.

Fig. 5 in Description of a New Brackish Water Ciliate, Uronychia xinjiangensis n. sp. (Ciliophora, Euplotida) Based on Morphology, Morphogenesis and Molecular Phylogeny

Fig. 5. Photomicrographs of Uronychia xinjiangensis n. sp. after protargol staining (A–I). (A–C, I) Ventral and dorsal views of specimens at interphase, showing ciliature and macronuclear nodules, arrow showing the small left marginal cirrus. (D, E) Anterior view, to show AZM1 and frontal cirri. (F) Arrows show two fine ventral cirri. (G, H) Depicting AZM2 and left marginal cirri. AZM1,2, anterior and proximal part of adoral zone of membranelles; CC, caudal cirri; DK3, dorsal kinety 3; FC, frontal cirri; LMC, left marginal cirri; Ma, macronuclear nodule; PM, paroral membrane; TC, transverse cirri. Scale bars: 20 μm.

Fig. 3 in Description of a New Brackish Water Ciliate, Uronychia xinjiangensis n. sp. (Ciliophora, Euplotida) Based on Morphology, Morphogenesis and Molecular Phylogeny

Fig. 3. Morphogenesis of Uronychia xinjiangensis n. sp. after protargol staining (A–F). (A, B) Ventral and dorsal view of the same late divider, showing migration of new structures and the formation of spherical fused macronucleus. (C, D) Ventral and dorsal view of the same late divider, five new membranelles combine with six retained membranelles to form anterior part of adoral zone of membranelles in the proter, the macronucleus dividing once. (E, F) Ventral and dorsal view of the same daughter cell just after fusion, showing infraciliature and nuclear apparatus. Scale bars: 20 μm.

Fig. 2 in Description of a New Brackish Water Ciliate, Uronychia xinjiangensis n. sp. (Ciliophora, Euplotida) Based on Morphology, Morphogenesis and Molecular Phylogeny

Fig. 2. Morphogenesis of Uronychia xinjiangensis n. sp. after protargol staining (A–F). (A, B) Ventral and dorsal view of the same specimen, arrows in A and B show cirral anlagen and two caudal cirri in the rightmost dorsal kinety anlagen of both proter and opisthe, respectively, arrowheads in A and B mark left marginal anlagen and replication bands, respectively. (C) Ventral view of a middle divider, arrowheads mark the development of left marginal anlagen. (D) Ventral view of a middle divider, showing the formation of paroral membrane anlagen and the segmentation of cirral anlagen (arrows), dedifferentiation of the old paroral membrane. (E, F) Ventral and dorsal view of the same late divider, showing the formation of new membranelles and cirri and other dorsal kineties anlagen, arrows show de novo formation of a frontal cirrus beside new paroral membrane OP, opisthe's oral primordium; PMA, paroral membrane anlage; POP, proter's oral primordium. Scale bars: 20 μm.

Fig. 1. Uronychia xinjiangensis n in Description of a New Brackish Water Ciliate, Uronychia xinjiangensis n. sp. (Ciliophora, Euplotida) Based on Morphology, Morphogenesis and Molecular Phylogeny

Fig. 1. Uronychia xinjiangensis n. sp. in vivo (A) and after protargol staining (B–F). (A) Ventral view of a representative individual. (B, C) Ventral and dorsal view of the holotype specimen, showing the ciliature and nuclear apparatus. Frontal-midventral-transverse cirri which originate from the same anlage are connected by a broken line. (D) Ventral view of an early divider, showing the formation of cirral anlagen and OP. (E, F) Ventral and dorsal view of the same early divider, showing the development of cirral anlagen and OP as well the formation of proter's oral primodirum, left marginal anlagen (arrows in E) and replication bands (arrow in F) and dorsal kinety anlagen in the two rightmost old structures; arrowhead indicates short cirral anlage. AZM1,2, anterior and proximal part of adoral zone of membranelles; BC, buccal cirrus; CA, cirral anlagen; CC, caudal cirri; DK1-3, dorsal kineties 1–3; FC, frontal cirri; LMC, left marginal cirri; Ma, macronuclear nodule; OP, opisther's oral primodium; PM, paroral membrane; POP, proter's oral primordium; TC, transverse cirri; VC, ventral cirri. Scale bars: 20 μm.

Fig. 6 in Morphology of Three Aloricate Choreotrich ciliates, Including Description of a New Species Parastrombidinopsis costalis sp. n. (Ciliophora, Choreotrichia), and phylogeny of the genus Parastrombidinopsis

Fig. 6. Drawings and photomicrographs of Parastrombidinopsis minima from life (A, E–G) and after protargol staining (B–D, H–M). (A, F, G) Views of three individuals showing the body shape. (B, C) Ventral and dorsal views of the same specimen showing the ciliature. (D, H, I) Detail of collar membranelles; the arrows mark the ventral gap. (E) From Tsai et al. (2008). (J) Somatic ciliature. (K) An early divider; arrowhead marks the oral primordium. (L, M) Ventral and dorsal views of same specimen showing the ciliature. CM, collar membranelles; E, endoral membrane; SK, somatic kinety. Scale bars: 25 μm.

Fig. 7 in Morphology of Three Aloricate Choreotrich ciliates, Including Description of a New Species Parastrombidinopsis costalis sp. n. (Ciliophora, Choreotrichia), and phylogeny of the genus Parastrombidinopsis

Fig. 7. Bayesian-Inference tree inferred from SSU rRNA gene sequences, indicating the polygenetic positions of species of the genus Parastrombidinopsis. Numbers at the nodes represent support values in the following order: BI posterior probabilities and ML bootstrap values. Disagreements in topology between the BI and ML trees are indicated by a hyphen. Nodes that were well supported (1.00 BI; 100% ML) are represented by filled circles. Bar = 5 substitutions per 100 nucleotide positions. Species sequenced in the present study are shown in bold type.

Fig. 4 in Morphology of Three Aloricate Choreotrich ciliates, Including Description of a New Species Parastrombidinopsis costalis sp. n. (Ciliophora, Choreotrichia), and phylogeny of the genus Parastrombidinopsis

Fig. 4. Parastrombidinopsis pelagica (Fauré-Fremiet, 1924) comb. n. from life (A–D, F) and after protargol staining (E, G–I). (A, B) Ventral views of two individuals. (C) Swimming pattern. (D) Different individuals to show the variations in body shape. (E) Detail of oral membranelles. (F) From Fauré-Fremiet (1924). (G, H) Ventral and dorsal views of the same specimen showing the ciliature. (I) Lateral view of a specimen. CM, collar membranelles; Ma, macronucleus; Mi, micronucleus; SK, somatic kinety. Scale bars: 50 μm.

Fig. 3 in Morphology of Three Aloricate Choreotrich ciliates, Including Description of a New Species Parastrombidinopsis costalis sp. n. (Ciliophora, Choreotrichia), and phylogeny of the genus Parastrombidinopsis

Fig. 3. Photomicrographs of Parastrombidinopsis costalis sp. n. from life (A–F, J) and after protargol staining (G–I, K–N), (A–E, G–J, L, M) from Zhuhai population, and (F, K, N) from Sanya population. (A–C) Ventral-left, ventral, left-lateral views of one individual, arrows mark the dorsal ridge. (D, E) Ventral-left, ventral-right views of one individual; arrows mark the dorsal ridge. (F) Ventral-right view of one individual, arrow marks the dorsal ridge. (G, H) Ventral and dorsal views of one specimen, showing the somatic ciliature. (I) Ventral view of oral ciliature; arrow marks buccal membranelle. (J) Bases of oral membranelles; arrow marks buccal membranelle. (L) An early divider; arrow marks the oral primordium. (M) Two macronuclei. (K, N) Ventral and dorsal views of one specimen, showing the somatic ciliature. CM, collar membranelles; Ma, macronucleus; SK, somatic kinety. Scale bars: 25 μm (A, D); 20 μm (F); 3 μm (J).

Fig. 2 in Morphology of Three Aloricate Choreotrich ciliates, Including Description of a New Species Parastrombidinopsis costalis sp. n. (Ciliophora, Choreotrichia), and phylogeny of the genus Parastrombidinopsis

Fig. 2. Parastrombidinopsis costalis sp. n. of Zhuhai population from life (A–D) and after protargol staining (E–G). (A, B) Ventral and left lateral views of representative individuals; arrows mark the dorsal ridges. (C) Apical view, showing collar membranelles and body shape; arrow marks the dorsal ridge. (D) Locomotion. (E) Oral ciliature. (F, G) Ventral and dorsal views of the same specimen. BM, buccal membranelle; CM, collar membranelles; E, endoral membrane; SK, somatic kinety. Scale bars: 25 μm.

Fig. 1 in Morphology of Three Aloricate Choreotrich ciliates, Including Description of a New Species Parastrombidinopsis costalis sp. n. (Ciliophora, Choreotrichia), and phylogeny of the genus Parastrombidinopsis

Fig. 1. Satellite photograph of South-East China (A) and pictures of sampling sites (B–E). (A) The position of Zhanjiang, Zhuhai, Haikou and Sanya. (B) Coastal waters off Zhanjiang. (C) Coastal waters off Zhuhai. (D) Brackish waters in Haikou. (E) Mangrove wetland in Sanya.