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FIGURE 9 in Morphological description and DNA barcodes of shallow-water Tetractinellida (Porifera: Demospongiae) from Bocas del Toro, Panama, with description of a new species

FIGURE 9. Stelletta fibrosa (Schmidt, 1870) [ZMBN 81784]: A. Plagiotriaene and oxeas. Scale: 100 µm; B. Anatriaene. Scale: 20 µm; C–D. Tylaster. Scale: 2 µm; S. fibrosa [MCZ 8020, lectotype]: E. Plagiotriaene. Scale: 20 µm; F. Tylaster. Scale: 2 µm.

FIGURE 10 in Morphological description and DNA barcodes of shallow-water Tetractinellida (Porifera: Demospongiae) from Bocas del Toro, Panama, with description of a new species

FIGURE 10. Stelletta sp. [ZMBN 81643]: A. Specimen in situ on mangrove root, August 2007. Notice large oscule leading to a cloaca. Scale: 2 cm; B. Bleached specimen maintained alive in a tank, July 2009. Scale: 2 cm; C. Cross– section showing the skeletal architecture. Scale: 1 mm; D. Close-up of cross-section showing bundles of triaenes at the surface of the sponge. Scale: 1 mm; E. Oxea II. Scale: 100 µm; F. Plagiotriaenes. Scale: 100 µm; G. Anatriaene and tylaster. Scale: 20 µm; H. Tylaster. Scale: 4 µm.

FIGURE 6 in Morphological description and DNA barcodes of shallow-water Tetractinellida (Porifera: Demospongiae) from Bocas del Toro, Panama, with description of a new species

FIGURE 6. Ecionemia megastylifera Wintermann-Kilian & Kilian, 1984 [ZMBN 81782]: A. Microrhabd and acanthostrongylaster. Scale: 10 µm; B. Acanthoxyaster. Scale: 2 µm; C. Acanthostrongylaster. Scale: 2 µm; E. megastylifera [INVPOR 1148, holotype]: D. Young triaene. Scale: 20 µ m; E. Microxea, microrhabd and acanthostrongylaster. Scale: 10 µm; F. Acanthostrongylasters. Scale: 2 µm.

FIGURE 12 in Morphological description and DNA barcodes of shallow-water Tetractinellida (Porifera: Demospongiae) from Bocas del Toro, Panama, with description of a new species

FIGURE 12. Spicule repertoire of Stryphnus raratriaenus sp. nov [ZMBN 81642]: A. Oxea I; B. Oxea II; C. Style; D. Dichotriaene; E. Oxyasters; F. Sanidasters. Scale 200 µm for A. Scale 50 µm for B–C–D. Scale 10 µm for E–F.

FIGURES 63–76 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURES 63–76. Larva of Simulium litobranchium n. sp. (Diptera: Simuliidae). 63–70. Variation in the head color. 63. Head capsule, dorsal view. 64. Head capsule, ventral view. 65. Head capsule, dorsal view. 66. Head capsule, ventral view. 67. Dorsal view of cephalic apotome and cervical sclerites. 68. Postgenal cleft, ventral view. 69. Dorsal view of cephalic apotome and cervical sclerites. 70. Postgenal cleft, ventral view. 71. Hypostoma. 72. Antenna. 73. Lanceolated setae on body cuticle. 74. Anal sclerite. 75. Papillae of one of the three lobes of rectal papilla. 76. Dorsal tubercles on cuticle of abdominal segment.

FIGURES 59–62 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURES 59–62. Lateral view of Simulium litobranchium n. sp. larvae (Diptera: Simuliidae) showing color variation and variation in the development and number of paired dorsal tubercles.

FIGURES 45–50 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURES 45–50. Pupa of Simulium litobranchium n. sp. (Diptera: Simuliidae). 45. Pupa inside cocoon, lateral view. 46. Middle region of the anterior margin of the cocoon, in dorsal view. 47. Male cephalic plate. 48. Head capsule frontal trichome. 49. Head capsule dorsal trichome. 50. Thoracic trichomes.

FIGURES 17–25. Male. 17–19 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURES 17–25. Male. 17–19. Simulium guianense (Diptera: Simuliidae). 17. Scutum, dorsal view, anterior illumination. 18. Scutum, dorsal view, posterior illumination. 19. Scutum, dorso-lateral view. 20–25. Simulium litobranchium n. sp. 20–22. Goias state population. 20. Scutum, dorsal view, anterior illumination. 21. Scutum, dorsal view, posterior illumination. 22. Scutum, dorso-lateral view. 23–25. Minas Gerais state population. 23. Scutum, dorsal view, anterior illumination. 24. Scutum, dorsal view, posterior illumination. 25. Scutum, dorso-lateral view.

FIGURES 1–11 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURES 1–11. Female of Simulium litobranchium n. sp. (Diptera: Simuliidae). 1. Scutum, dorsal view, anterior illumination. 2. Scutum, dorsal view, posterior illumination. 3. Cibarium. 4. Fronto-ocular triangle. 5. Maxillary palp. 6. Wing base. 7. Fore leg. 8. Middle leg. 9. Hind leg. 10. Calcipala and pedisulcus. 11. Tarsal claw from middle leg.

FIGURES 57–58 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURES 57–58. Pupa of Simulium litobranchium n. sp. (Diptera: Simuliidae). 57. Abdomen, dorsal view. 57a–c: setae and hooks on tergites II–IV. 57d: group of spine combs on tergites VI–IX. 58. Abdomen, ventral view. 58a: group of spine combs on sternites III–VIII. 58b–d: hooks on sternites V–VII.

FIGURES 33–44 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURES 33–44. Male terminalia and ventral plate. Simulium duodenicornium (Diptera: Simuliidae). 33. Terminalia, ventral view. 36. Ventral plate, ventral view. 39. Ventral plate, in ventral view tilted dorsally. 42. Ventral plate, lateral view. Simulium guianense. 34. Terminalia, ventral view. 37. Ventral plate, ventral view. 40. Ventral plate, in ventral view tilted dorsally. 43. Ventral plate, lateral view. Simulium litobranchium n. sp. 35. Terminalia, ventral view. 38. Ventral plate, ventral view. 41. Ventral plate, in ventral view tilted dorsally. 44. Ventral plate, lateral view. [Modified from Pepinelli et al. (2005)]

FIGURES 12–16 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURES 12–16. Female of Simulium litobranchium n. sp. (Diptera: Simuliidae). 12. Cercus and anal lobe in situ, ventral view. 13. Cercus and anal lobe, mounted between slide and coverslip, lateral view. 14. Hypogynial valves, ventral view. 15. Genital fork. 16. Cuticular microspines of spermatheca.

FIGURES 51–56 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURES 51–56. Pupal gill filaments and apex of gills. Figs 51–52. Simulium litobranchium n. sp. (Diptera: Simuliidae). Figs 53–54. Simulium guianense. Figs 55–56. Simulium duodenicornium.

FIGURES 26–32 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURES 26–32. Male of Simulium litobranchium n. sp. (Diptera: Simuliidae). 26. Wing base. 27. Antenna. 28. Maxillary palp. 29. Median sclerite. 30. Aedeagal membrane and paramere. 31. Gonocoxite and gonostylus, mounted between slide and coverslip, lateral view. 32. Spinule at the apex of gonostylus.

FIGURE 77. A Kimura 2 in A new black fly species from Brazil, closely related to Simulium guianense Wise (Diptera, Simuliidae), revealed by morphology and DNA barcoding

FIGURE 77. A Kimura 2-parameter neighbor-joining tree for 4 nominal species of Neotropical black flies S. (Thyrsopelma) subgenus. Scale bar = 2%

FIGURE 9 in Description and DNA barcoding assessment of the new species Deutonura gibbosa (Collembola: Neanuridae: Neanurinae), a common springtail of Alps and Jura

FIGURE 9. Neighbor joining tree (K2P) of five species of the phlegraea group of the genus Deutonura based on the COI 5' 'barcoding fragment'. Bootstrap support values showed on the branches. The upper and lower side of the triangle represent respectively the maximum and minimum of genetic distances within the species.

FIGURES 7–8 in Description and DNA barcoding assessment of the new species Deutonura gibbosa (Collembola: Neanuridae: Neanurinae), a common springtail of Alps and Jura

FIGURES 7–8. Arrangement of tubercles and chaetae on the tergite of Abd. V–VI in a paratype of Deutonura deficiens sylvatica (Fig. 7) and in a paratype of D. gibbosa sp. nov. (Fig. 8, Abd. VI not visible in dorsal view); 1, 2, 3: chaetae Di1, Di2 and Di3 on Abd. V.

FIGURES 2–6 in Description and DNA barcoding assessment of the new species Deutonura gibbosa (Collembola: Neanuridae: Neanurinae), a common springtail of Alps and Jura

FIGURES 2–6. Deutonura gibbosa sp. nov.: 2, dorsal chaetotaxy and tubercles (represented by their undercuticular reticulations); 3, labrum; 4, labium (l—lateral chaeta of labrum); 5, tibiotarsus and claw of leg I (dotted line: limit of secondary granules on dorsal side); 6, ventral chaetotaxy of abdomen. Muscular insertions as punctuated areas; pp: pseudopora.

FIGURE 1 in Description and DNA barcoding assessment of the new species Deutonura gibbosa (Collembola: Neanuridae: Neanurinae), a common springtail of Alps and Jura

FIGURE 1. Distribution map of Deutonura gibbosa sp. nov. (triangles, Alps and southern border of Jura) and D. deficiens sylvatica (circles, western France, northwestern Spain and Sardinia)). Large triangle and large circle: type localities; only localities from which we have examined specimens are represented for Sardinia.