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Figure 9 from: Smit J, Reijnen B, Stokvis F (2013) Half of the European fruit fly species barcoded (Diptera, Tephritidae); a feasibility test for molecular identification. ZooKeys 365: 279-305. https://doi.org/10.3897/zookeys.365.5819

Figure 9 - The Neighbour-Joining tree of the genus Orellia inferred from COI barcodes. Bootstrap values above 50 (1000 replicates) are given at the nodes.

Figure 6 from: Smit J, Reijnen B, Stokvis F (2013) Half of the European fruit fly species barcoded (Diptera, Tephritidae); a feasibility test for molecular identification. ZooKeys 365: 279-305. https://doi.org/10.3897/zookeys.365.5819

Figure 6 - Best Close Match (BCM) identification of the stripped dataset, e.g. excluding singletons and Urophora (n = 414). Proportions of true positives (TP), false positives (FP), false negatives (FN) and true negatives (TN) are given for 30 arbitrary distance thresholds ranging from 0.15 to 0.00. For each threshold the percentages of precision, accuracy and discarded queries were calculated.

Figure 5 from: Smit J, Reijnen B, Stokvis F (2013) Half of the European fruit fly species barcoded (Diptera, Tephritidae); a feasibility test for molecular identification. ZooKeys 365: 279-305. https://doi.org/10.3897/zookeys.365.5819

Figure 5 - Best Close Match (BCM) identification of the entire dataset (n = 555). Proportions of true positives (TP), false positives (FP), false negatives (FN) and true negatives (TN) are given for 30 arbitrary distance thresholds ranging from 0.15 to 0.00. For each threshold the percentages of precision, accuracy and discarded queries were calculated.

Figure 4 from: Smit J, Reijnen B, Stokvis F (2013) Half of the European fruit fly species barcoded (Diptera, Tephritidae); a feasibility test for molecular identification. ZooKeys 365: 279-305. https://doi.org/10.3897/zookeys.365.5819

Figure 4 - Identification rates of all five criteria: Neighbour-Joining (NJT) sensu Hebert et al. (2003), revised criteria (NJT_M) according to Meier et al. (2006), and Best Match (BM), Best Close Match (BCM) and All Species Barcodes (ASB) also described by Meier et al. (2006) for four different datasets, including singletons and with (n = 555) or without (n = 452) Urophora, and the same excluding singletons (n = 514) and (n = 414) respectively.

Figure 3 from: Smit J, Reijnen B, Stokvis F (2013) Half of the European fruit fly species barcoded (Diptera, Tephritidae); a feasibility test for molecular identification. ZooKeys 365: 279-305. https://doi.org/10.3897/zookeys.365.5819

Figure 3 - Box plots depicting the variation in mean distances using K2P-distance modeling of sequence divergence for intraspecific, interspecific difference among the species and genera, as well as the ingroup genera with the outgroup genus.

Figure 7 from: Smit J, Reijnen B, Stokvis F (2013) Half of the European fruit fly species barcoded (Diptera, Tephritidae); a feasibility test for molecular identification. ZooKeys 365: 279-305. https://doi.org/10.3897/zookeys.365.5819

Figure 7 - Relative ID errors at 30 arbitrary threshold values for a. the entire dataset (n = 555), b. the stripped dataset, e.g. excluding singletons and Urophora (n = 414) and c. the stripped dataset excluding the problematic Terellia groups. Linear regression was used to infer the ad hoc threshold for the 95th percentile of the correctly identified queries and the relative ID error does not exceed 5%. In (a) and (b) this value is below 0.00, only in (c) this value is positive: 0.051 (R-square 0.91).

Figure 2 from: Smit J, Reijnen B, Stokvis F (2013) Half of the European fruit fly species barcoded (Diptera, Tephritidae); a feasibility test for molecular identification. ZooKeys 365: 279-305. https://doi.org/10.3897/zookeys.365.5819

Figure 2 - The Neighbour-Joining tree of the entire dataset based on COI barcodes. Terminal branches have been collapsed in order to save space, the total number of specimens is given in brackets and the area surface of the triangle represents the amount of variation. When a terminal branch contains two species, both names are provided as well as their respective number of specimens. If a branch contains more than two species only the number of species as well as the number of specimens are given. Bootstrap values above 50 (1000 replicates) are given at the nodes.

Figure 10 from: Smit J, Reijnen B, Stokvis F (2013) Half of the European fruit fly species barcoded (Diptera, Tephritidae); a feasibility test for molecular identification. ZooKeys 365: 279-305. https://doi.org/10.3897/zookeys.365.5819

Figure 10 - The Neighbour-Joining tree of the genus Urophora inferred from COI barcodes. Bootstrap values above 50 (1000 replicates) are given at the nodes.

Figure 1 from: Juárez ML, Devescovi F, Břízová R, Bachmann G, Segura DF, Kalinová B, Fernández P, Ruiz MJ, Yang J, Teal PEA, Cáceres C, Vreysen MJB, Hendrichs J, Vera MT (2015) Evaluating mating compatibility within fruit fly cryptic species complexes and the potential role of sex pheromones in pre-mating isolation. In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 125-155. https://doi.org/10.3897/zookeys.540.6133

Figure 1 - Walk-in field cage set up to evaluate female response to male pheromone: a artificial lek hanging from the tree b Anastrepha fraterculus female over an artificial lek.

Figure 2 from: Tanga CM, Manrakhan A, Daneel JH, Mohamed SA, Khamis FM, Ekesi S (2015) Comparative analysis of development and survival of two Natal fruit fly Ceratitis rosa Karsch (Diptera, Tephritidae) populations from Kenya and South Africa. In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 467-487. https://doi.org/10.3897/zookeys.540.9906

Figure 2 - Linear and non-linear regressions of temperature related developmental rates of immature stages of two groups of Ceratitis rosa from South Africa

Figure 1 from: Tanga CM, Manrakhan A, Daneel JH, Mohamed SA, Khamis FM, Ekesi S (2015) Comparative analysis of development and survival of two Natal fruit fly Ceratitis rosa Karsch (Diptera, Tephritidae) populations from Kenya and South Africa. In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 467-487. https://doi.org/10.3897/zookeys.540.9906

Figure 1 - Linear and non-linear regressions of temperature related developmental rates of immature stages of two groups of Ceratitis rosa from Kenya.

Figure 4 from: Břízová R, Vaníčková L, Faťarová M, Ekesi S, Hoskovec M, Kalinová B (2015) Analyses of volatiles produced by the African fruit fly species complex (Diptera, Tephritidae). In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 385-404. https://doi.org/10.3897/zookeys.540.9630

Figure 4 - The results of statistical analyses of the male-borne volatiles produced by Ceratitis fasciventris (blue), Ceratitis anonae (green) and Ceratitis rosa (red). (A) Multivariate principal component analysis (PCA) of the 22 common compounds identified in the pheromone of the males of the FAR complex. (B) Multivariate correspondence analysis (CA) of the 12 antennal active compounds. The three species are clearly segregated. Each symbol on the plot represents one sample. The numbers in italics denote the retention indices (RI) of the species-specific compounds. For the structural identification of the compounds see the Suppl. materials 1–3: Tables 1–3.

Figure 3 from: Břízová R, Vaníčková L, Faťarová M, Ekesi S, Hoskovec M, Kalinová B (2015) Analyses of volatiles produced by the African fruit fly species complex (Diptera, Tephritidae). In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 385-404. https://doi.org/10.3897/zookeys.540.9630

Figure 3 - A comparison of female antennal responses of Ceratitis fasciventris, Ceratitis anonae, and Ceratitis rosa to standard solutions. The FID/EAD on the y-axis represents the ratio between an electroantennographic response and a conventional detector. The higher the number, the higher the response (N = 3).

Figure 2 from: Břízová R, Vaníčková L, Faťarová M, Ekesi S, Hoskovec M, Kalinová B (2015) Analyses of volatiles produced by the African fruit fly species complex (Diptera, Tephritidae). In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 385-404. https://doi.org/10.3897/zookeys.540.9630

Figure 2 - GC-FID/EAD analyses of the Ceratitis fasciventris, Ceratitis anonae, and Ceratitis rosa male-borne volatiles using a conspecific female antenna as an EAD detector. The numbers indicate EAD-active compounds and correspond to Table 1. The symbols EAD-1-3 denote the three independent repetitions of the GC-EAD analyses.

Figure 1 from: Břízová R, Vaníčková L, Faťarová M, Ekesi S, Hoskovec M, Kalinová B (2015) Analyses of volatiles produced by the African fruit fly species complex (Diptera, Tephritidae). In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 385-404. https://doi.org/10.3897/zookeys.540.9630

Figure 1 - GC×GC-TOFMS chromatograms (TIC mode) of the male (N = 5) volatiles of Ceratitis fasciventris, Ceratitis anonae and Ceratitis rosa. Each spot represents one compound; the identified compounds are numbered in each chromatogram, with the numbering corresponding to the respective Table 1 of compounds. The intensity of each spot is colour-coded (blue - 0, red - maximum).

Figure 3 from: Vaníčková L, Břízová R, Pompeiano A, Ekesi S, De Meyer M (2015) Cuticular hydrocarbons corroborate the distinction between lowland and highland Natal fruit fly (Tephritidae, Ceratitis rosa) populations. In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 507-524. https://doi.org/10.3897/zookeys.540.9619

Figure 3 - Section of the GC×GC/MS analysis of the female (A) and male (B) cuticular hydrocarbon profiles of coastal population (R1) of Ceratitis rosa from Kenya. The intensity of the signals is colour-coded from green (zero) to red (maximum). The compounds are assigned according to Table 1.

Figure 2 from: Vaníčková L, Břízová R, Pompeiano A, Ekesi S, De Meyer M (2015) Cuticular hydrocarbons corroborate the distinction between lowland and highland Natal fruit fly (Tephritidae, Ceratitis rosa) populations. In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 507-524. https://doi.org/10.3897/zookeys.540.9619

Figure 2 - Section of the GC×GC/MS analysis of the female (A) and male (B) cuticular hydrocarbon profiles of the highland population (R2) of Ceratitis rosa from Kenya. The intensity of the signals is colour-coded from green (zero) to red (maximum). The compounds are assigned according to Table 1.

Figure 1 from: Vaníčková L, Břízová R, Pompeiano A, Ekesi S, De Meyer M (2015) Cuticular hydrocarbons corroborate the distinction between lowland and highland Natal fruit fly (Tephritidae, Ceratitis rosa) populations. In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 507-524. https://doi.org/10.3897/zookeys.540.9619

Figure 1 - A heat map of the 46 cuticular hydrocarbons (columns, CH1-46) and the two Ceratitis rosa populations (rows, f-female, m-male) from the GC×GC/MS data set. The dendrograms are created using correlation-based distances and the Ward method of hierarchical clustering (P < 0.05). Putative morphotypes (R1 for the coastal population and R2 for the highland population) are depicted in the row dendrogram.

Figure 2 from: Virgilio M, Delatte H, Nzogela YB, Simiand C, Quilici S, De Meyer M, Mwatawala M (2015) Population structure and cryptic genetic variation in the mango fruit fly, Ceratitis cosyra (Diptera, Tephritidae). In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 525-538. https://doi.org/10.3897/zookeys.540.9618

Figure 2 - Individual Bayesian assignments. STRUCTURE sequential individual assignments of 348 specimens of Ceratitis cosyra from 13 African countries.

Figure 1 from: Virgilio M, Delatte H, Nzogela YB, Simiand C, Quilici S, De Meyer M, Mwatawala M (2015) Population structure and cryptic genetic variation in the mango fruit fly, Ceratitis cosyra (Diptera, Tephritidae). In: De Meyer M, Clarke AR, Vera MT, Hendrichs J (Eds) Resolution of Cryptic Species Complexes of Tephritid Pests to Enhance SIT Application and Facilitate International Trade. ZooKeys 540: 525-538. https://doi.org/10.3897/zookeys.540.9618

Figure 1 - Unconstrained and constrained ordination. Principal Component Analysis (PCA) and Discriminant Analysis of Principal Components (DAPC) of 348 Ceratitis cosyra microsatellite genotypes. Specimen groups are labelled inside their 95% inertia ellipses and genotypes are connected to the corresponding group centroids.