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89 results for “Chlamydomonas reinhardtii”
Change in gene expression in Chlamydomonas reinhardtii upon heat shock and feeding with hemin and Mg-protoporphyrin
GEO Series GSE20861. Chlamydomonas reinhardtii. 40 samples. Type: Expression profiling by array.
Data from: An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii
The green alga Chlamydomonas reinhardtii is a leading single-celled model for dissecting biological processes in photosynthetic eukaryotes. However, its usefulness has been limited by difficulties in obtaining mutants in genes of interest. To allow generation of large numbers of mapped mutants, we developed high-throughput methods which: (1) Enable easy propagation on agar and cryogenic maintenance of tens of thousands of C. reinhardtii strains; (2) Identify mutant insertion sites and physical coordinates in such collections; (3) Validate the insertion sites in pools of mutants by obtaining >500 bp of flanking genomic sequences. We used these approaches to construct a stably maintained library of 1,935 mapped mutants, representing disruptions in 1,562 genes. We further characterized randomly selected mutants, and found that 33 out of 44 insertion sites (75%) could be confirmed by PCR, and 17 out of 23 mutants (74%) contained a single insertion. To demonstrate the power of this library for elucidating biological processes, we analyzed the lipid content of mutants disrupted in genes encoding proteins of the algal lipid droplet proteome. This study revealed a central role of the long-chain acyl-CoA synthetase LCS2 in the production of triacylglycerol from de novo synthesized fatty acids.
Oil extraction report from Chlamydomonas reinhardtii engineered to produce ketocarotenoids
<p>Report of oil extraction efficiency on <em>Chlamydomonas reinhardtii</em> strain engineered to produce ketocarotenoids and astaxanthin.</p> <p> </p> <p><strong>Oil report on lab scale:</strong></p> <p>Oil extraction was performed as described in Perozeni et al. Plant Biotechnology Journal 2020, starting from different volumes of saturated culture (300, 600, 900, and 1800 ml). The cells were pelleted and dissolved in 25 ml of sunflower oil. The extraction was thus performed on wet biomass. After centrifugation, the oil extract was diluted in oil and the absorption spectrum was used to determine the ketocarotenoid concentration as described in Perozeni et al. Plant Biotechnology Journal 2020.</p> <p> </p> <p>The spectra recorded are in the sheet “Raw”. In the sheets “Quantification” there are the results of the ketocarotenoids quantification and the extraction efficiency. In the sheet "summary" the overall results in terms of starting material, final oil volume, ketocarotenoid concentration and extraction efficiency are presented.</p> <p>Samples:</p> <p>1x: extraction starting from 300ml of C. reinhardtii EN1 strain saturated culture, oil extract diluted 1:10 before measuring the absorption spectrum.</p> <p>2x: extraction starting from 600ml of C. reinhardtii EN1 strain saturated culture, oil extract diluted 1:100 before measuring the absorption spectrum.</p> <p>3x: extraction starting from 900ml of C. reinhardtii EN1 strain saturated culture, oil extract diluted 1:100 before measuring the absorption spectrum.</p> <p>6x: extraction starting from 1800ml of C. reinhardtii EN1 strain saturated culture, oil extract diluted 1:1000 before measuring the absorption spectrum.</p> <p>6x 3<sup>rd</sup> cycle: extraction starting from 5400ml of C. reinhardtii EN1 strain saturated culture in three extraction steps recycling in each step the oil used for extraction to increase ketocarotenoid concentration, oil extract diluted 1:1000 before measuring the absorption spectrum.</p> <p>6x 4<sup>th</sup> cycle: extraction starting from 7200ml of C. reinhardtii EN1 strain saturated culture in three extraction steps recycling in each step the oil used for extraction to increase ketocarotenoid concentration, oil extract diluted 1:1000 before measuring the absorption spectrum.</p> <p>6x 5<sup>th</sup> cycle: extraction starting from 9000ml of C. reinhardtii EN1 strain saturated culture in three extraction steps recycling in each step the oil used for extraction to increase ketocarotenoid concentration, oil extract diluted 1:1000 before measuring the absorption spectrum.</p> <p>6x 6<sup>th</sup> cycle: extraction starting from 10800ml of C. reinhardtii EN1 strain saturated culture in three extraction steps recycling in each step the oil used for extraction to increase ketocarotenoid concentration, oil extract diluted 1:1000 before measuring the absorption spectrum.</p> <p> </p> <p> </p> <p><strong>Oil extraction report on large scale:</strong></p> <p>Oil extraction was performed according to the procedure developed for lab scale starting from 200 gr of total dry biomass (corresponding to a volume of 250L of a saturated cell culture). The overall biomass was extracted in three independent extractions (Extraction 1, 2, and 3), recycling the oil extract for up to 4th cycle, keeping the same ratio of oil/biomass adopted in the lab scale experiment. In the case of Extraction 1 the extraction was performed on wet biomass, while Extraction 2 and 3 were performed on dry biomass. After Extractions 1 and 2, the final pellet obtained was further extracted in oil (samples “ 2nd extraction 1” and “2nd extraction 2”). The spectra recorded are in the sheet “Raw”. In the sheet “Quantification” there are the results of the ketocarotenoids quantification considering the dilution (in oil) applied to the oil extracts. </p> <p>In the sheet “extraction eff." the finalresults in terms of starting material, final oil volume, ketocarotenoid concentration and extraction efficiency are presented. The overall extraction efficiency obtained considering all the extraction performed and the total oil extracts obtained is also reported </p> <p> </p>
Transcriptome profiling of the Chlamydomonas reinhardtii phot mutant
GEO Series GSE227473. Chlamydomonas reinhardtii. 12 samples. Type: Expression profiling by high throughput sequencing.
The biosynthesis of nitrous oxide in the green algae Chlamydomonas reinhardtii
GEO Series GSE90609. Chlamydomonas reinhardtii. 18 samples. Type: Expression profiling by high throughput sequencing.
High-throughput sequencing of small RNAs in Chlamydomonas reinhardtii
GEO Series GSE32457. Chlamydomonas reinhardtii. 3 samples. Type: Non-coding RNA profiling by high throughput sequencing.
Cell Cycle by Light Intensity in Chlamydomonas reinhardtii
GEO Series GSE275433. Chlamydomonas reinhardtii. 45 samples. Type: Expression profiling by high throughput sequencing.
Data from: An indexed, mapped mutant library enables reverse genetics studies of biological processes in Chlamydomonas reinhardtii
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Zinc deficiency impacts CO2 assimilation and disrupts copper homeostasis in Chlamydomonas reinhardtii
GEO Series GSE41096. Chlamydomonas reinhardtii. 1 samples. Type: Expression profiling by high throughput sequencing.
The Path to Triacylglyceride Obesity in the sta6 Strain of Chlamydomonas reinhardtii
GEO Series GSE55253. Chlamydomonas reinhardtii. 34 samples. Type: Expression profiling by high throughput sequencing.
Global transcriptome analysis of heterodimeric homeobox-driven zygote developmental program in Chlamydomonas reinhardtii
GEO Series GSE91400. Chlamydomonas reinhardtii. 8 samples. Type: Expression profiling by high throughput sequencing.
Endogenous small RNAs from Chlamydomonas reinhardtii strain J3(mt-) vegetative cells
GEO Series GSE7575. Chlamydomonas reinhardtii. 1 samples. Type: Non-coding RNA profiling by high throughput sequencing.
ChIP-seq of H3K4me1, H3K4me2, H3K4me3,H3K36me1, H3K36me2, and H3K36me3 for Chlamydomonas reinhardtii.
GEO Series GSE266902. Chlamydomonas reinhardtii. 14 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Genome-based approaches to understanding phosphorus deprivation responses and PSR1 control in Chlamydomonas reinhardtii.
GEO Series GSE4568. Chlamydomonas reinhardtii. 45 samples. Type: Expression profiling by array.
The soluble guanylate cyclase CYG12 is required for the acclimation to hypoxia and trophic regimes in Chlamydomonas reinhardtii
GEO Series GSE98442. Chlamydomonas reinhardtii. 12 samples. Type: Expression profiling by high throughput sequencing.
Base-resolution profiling of 5-glyceryl-methylcytosine in Chlamydomonas reinhardtii via deaminase-assisted sequencing [RNA-Seq]
GEO Series GSE301797. Chlamydomonas reinhardtii. 9 samples. Type: Expression profiling by high throughput sequencing.
High throughput sequencing data for Oryza sativa, Chlamydomonas reinhardtii, and Physcomitrella patens
GEO Series GSE266911. Physcomitrium patens; Chlamydomonas reinhardtii; Oryza sativa. 196 samples. Type: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing.
High-throughput Identification of Novel Heat Tolerance Genes via Genome-wide Pooled Mutant Screens in the Model Green Alga Chlamydomonas reinhardtii
GEO Series GSE221132. Chlamydomonas reinhardtii. 26 samples. Type: Other.
Transcriptomics study of excess Mn (1000 uM) in Chlamydomonas reinhardtii
GEO Series GSE169756. Chlamydomonas reinhardtii. 6 samples. Type: Expression profiling by high throughput sequencing.
Phosphate Starvation in Chlamydomonas reinhardtii
GEO Series GSE56505. Chlamydomonas reinhardtii. 2 samples. Type: Expression profiling by high throughput sequencing.
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International Brain Laboratory public data
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OpenNeuro
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