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Figure 2 in Molecular data on Phyllodistomum macrocotyle (Digenea: Gorgoderidae) from an intermediate host Dreissena polymorpha (Bivalvia: Dreissenidae) in the Northern Dvina River Basin, Northwest Russia

Figure 2. Maximum likelihood phylogeny of Phyllodistomum macrocotyle based on the nuclear dataset (28S rDNA gene fragment). Numbers near nodes are bootstrap support (BS) values of IQ-TREE. Scale bar indicates the branch lengths. The red color indicates our sequence from Northwest Russia.

Fig. 4 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 4. Metacercariae of Diplostomum spp. from eye lenses of different fish hosts; (a) Diplostomum sp. from Tilipia sparrmanii, live, ventral view (b) Diplostomum sp. from Tilipia sparrmanii, fixed, ventral view (c) Diplostomum sp. from Tilipia sparrmanii, live, sunken pseudosuckers (arrowhead) (hologenophore, GenBank MN813526, MN813534 and MN808616) (d) Diplostomum sp. 14 sensu Locke et al. (2015) from Synodontis zambezensis, live, ventral view (hologenophore, GenBank MN813541) (e) Diplostomum sp. 14 sensu Locke et al. (2015) from Oreochromis mossambicus, fixed, ventral view, small excretory granules (arrowhead) (hologenophore, GenBank MN813531, MN813539 and MN808621) (f) Diplostomum sp. 14 sensu Locke et al. (2015) from Synodontis zambezensis, fixed, ventral view, large excretory granules (arrowhead) (hologenophore, GenBank MN813541) (g) Diplostomum sp. 16 sensu Locke et al. (2015) from Pseudocrenilabrus philander, fixed, ventral view, everted pseudosuckers (arrowhead) (hologenophore, GenBank MN813532, MN813547 and MN808627) (h) Diplostomum sp. 16 sensu Locke et al. (2015) from Pseudocrenilabrus philander, fixed, ventral view, inverted pseudosuckers (arrowhead) (hologenophore, GenBank MN813533, MN813548 and MN808628) (i) Diplostomum sp. 16 sensu Locke et al. (2015) from Pseudocrenilabrus philander, live metacercariae inside of fish lens. Scale bars: a–h = 100 μm; i = 700 μm.

Fig. 3 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 3. Bayesian inference (BI) and maximum likelihood (ML) phylogram reconstructed using cox1 sequences for species of Diplostomum. Nodal support from BI and ML analyses indicated as BI/ML; only values> 0.90 (BI) and> 70 (ML) are displayed. Scale-bar indicates the expected number of substitution per site. Sequences generated in this study are in bold and indicated by blue rectangles. Codes with isolate information for newly generated sequences are provided in Table 3. Sequences derived from Africa are highlighted in blue, from Asia in purple, from Europe in orange, from North America in green (according to the map) and sequences reported from more than one continent are highlighted in black. Black arrows on the map demonstrate distribution of Diplostomum spathaceum and 'D. mergi Lineage 2' in both, Asia and Europe, and Diplostomum sp. 14 and Diplostomum sp. 16 in both, Africa and Asia. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 2 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 2. Bayesian inference (BI) and maximum likelihood (ML) phylograms reconstructed using (a) partial 28S rDNA sequences (b) ITS1-5.8S-ITS2 sequences for species of Diplostomum. Nodal support from BI and ML analyses indicated as BI/ML; only values> 0.90 (BI) and> 70 (ML) are displayed. Scale-bar indicates the expected number of substitution per site. Sequences generated in this study are in bold and indicated by blue rectangles. Codes with isolate information for newly generated sequences are provided in Table 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 in Resolution of the identity of three species of Diplostomum (Digenea: Diplostomidae) parasitising freshwater fishes in South Africa, combining molecular and morphological evidence

Fig. 1. Map illustrating the sampling localities on (a) River Riet in Mokala National Park (b) River Phongolo (Site 1, Site 2 and Nyamithi Lake) and the River Usuthu (Shokwe Pan) in Ndumo Game Reserve and (c) River Mooi (Boskop Dam) in Boskop Dam Nature Reserve, South Africa. The illustration was compiled in ArcGIS 10.6 (Available from https://support.esri.com/en/downloads).

Figure 2 in First record of metacercariae trematodes Opisthioglyphe ranae (Digenea: Telorchiidae) and Echinostoma bolschewense (Digenea: Echinostomatidae) in Dreissena polymorpha (Bivalvia: Dreissenidae) from the Don and Volga river basins, Russia

Figure 2. Maximum likelihood phylogeny of Echinostoma genus based on the nuclear dataset (28S rDNA gene fragment). Numbers near nodes are bootstrap support (BS) values of IQ-TREE. Scale bar indicates the branch lengths. Red color indicates our sequence from the Sokolovskoe Reservoir (Don River basin).

Figure 3 in First record of metacercariae trematodes Opisthioglyphe ranae (Digenea: Telorchiidae) and Echinostoma bolschewense (Digenea: Echinostomatidae) in Dreissena polymorpha (Bivalvia: Dreissenidae) from the Don and Volga river basins, Russia

Figure 3. Maximum likelihood phylogeny of Echinostoma genus based on the mitochondrial dataset (COI gene fragment). Numbers near nodes are bootstrap support (BS) values of IQ-TREE. Scale bar indicates the branch lengths. Red color indicates our sequence from the Sokolovskoe Reservoir (Don River basin) and Volga River.

Figure 7 in First record of metacercariae trematodes Opisthioglyphe ranae (Digenea: Telorchiidae) and Echinostoma bolschewense (Digenea: Echinostomatidae) in Dreissena polymorpha (Bivalvia: Dreissenidae) from the Don and Volga river basins, Russia

Figure 7. Encysted metacercariae of Opisthioglyphe ranae detected in Dreissena polymorpha from Seversky Donets River (Don River Basin, Russia) (A) Metacercarial cysts in the visceral mass of zebra mussel. (B) Encysted metacercaria.

Figure 1 in First record of metacercariae trematodes Opisthioglyphe ranae (Digenea: Telorchiidae) and Echinostoma bolschewense (Digenea: Echinostomatidae) in Dreissena polymorpha (Bivalvia: Dreissenidae) from the Don and Volga river basins, Russia

Figure 1. Map of study area. (A) Discovery of the Dreissena polymorpha in Volga and Don rivers basin, Russia: 1. Sokolovskoe reservoir (Don River basin), 2. Volga River, 3. Seversky Donets River (Don River basin); (B) View of the habitat of D. polymorpha (a) Sokolovskoe reservoir (photo by A. Tomilova), (C) Seversky Donets River (photo by A. Lyubas).

Figure 6 in First record of metacercariae trematodes Opisthioglyphe ranae (Digenea: Telorchiidae) and Echinostoma bolschewense (Digenea: Echinostomatidae) in Dreissena polymorpha (Bivalvia: Dreissenidae) from the Don and Volga river basins, Russia

Figure 6. Encysted metacercariae of Echinostoma bolschewense detected in Dreissena polymorpha from Volga and Don River basin, Russia (A) Metacercarial cysts in the gonad of zebra mussel. (B) Encysted metacercaria.

Figure 4 in First record of metacercariae trematodes Opisthioglyphe ranae (Digenea: Telorchiidae) and Echinostoma bolschewense (Digenea: Echinostomatidae) in Dreissena polymorpha (Bivalvia: Dreissenidae) from the Don and Volga river basins, Russia

Figure 4. Maximum likelihood phylogeny of Opisthioglyphe ranae based on the nuclear dataset (28S rDNA gene fragment). Numbers near nodes are bootstrap support (BS) values of IQ-TREE. Scale bar indicates the branch lengths. Red color indicates our sequence from the Seversky Donets River.

Figure 5 in First record of metacercariae trematodes Opisthioglyphe ranae (Digenea: Telorchiidae) and Echinostoma bolschewense (Digenea: Echinostomatidae) in Dreissena polymorpha (Bivalvia: Dreissenidae) from the Don and Volga river basins, Russia

Figure 5. Maximum likelihood phylogeny of Opisthioglyphe ranae based on the mitochondrial dataset (COI gene fragment). Numbers near nodes are bootstrap support (BS) values of IQ-TREE. Scale bar indicates the branch lengths. Red color indicates our sequence from the Seversky Donets River.

Fig. 5 in Life cycle truncation in Digenea, a case study of Neophasis spp. (Acanthocolpidae)

Fig. 5. Neophasis oculata metacercariae (A–F) and sexual adult (G). (A) General view of metacercaria (acetic carmine, DIC). (B–C) Histological sections, Mallory's trichrome stain, encysted metacercaria (B) and some of its inner structures (C). (D–F) SEM, ventral view (D) and magnified spines in the anterior (E) and posterior (F) regions. (G) Sexual adult (acetic carmine, DIC). Scale bars – 100 μm on A, B, D, G; 50 μm on C; 10 μm on E, F.Abbreviations: c – ceca; ci – cirrus; eg – eggs; ev – excretory vesicle; icy – inner cyst layer; ocy – outer cyst layer; os – oral sucker; ot – ootype; ov – ovary; pe – pigmented eyespots; ph – pharynx; sv – seminal vesicle; te – testes; ut – uterus; vi – vitelline follicles; vs – ventral sucker.

Fig. 2 in Life cycle truncation in Digenea, a case study of Neophasis spp. (Acanthocolpidae)

Fig. 2. Neophasis oculata intramolluscan stages: daughter redia (A), infective cercaria body structure (B) and general view (C).

Fig. 8 in Life cycle truncation in Digenea, a case study of Neophasis spp. (Acanthocolpidae)

Fig. 8. Phylogenetic position of Neophasis oculata and N. anarrhichae based on the concatenated 18S and 28S rDNA sequence data, inferred with Bayesian inference. Newly generated sequences are indicated in bold. Posterior probabilities are printed at nodes, followed by bootstrap values for the nodes that were also supported in the tree inferred with Maximum likelihood method. Scale bar shows the substitution rate. GenBank accession numbers for the 18S and 28S rDNA sequences are listed in the Supplementary Table S1.

Fig. 7 in Life cycle truncation in Digenea, a case study of Neophasis spp. (Acanthocolpidae)

Fig. 7. Neophasis anarrhichae successive life cycle stages. (A, B) whole mounts (toluidine blue, DIC) of the anterior end of daughter redia (A) and cercaria embryo (B). (C) Cercaria later embryo, CLSM, TRITC-phalloidin and phospho Y antibody staining. (D, E) whole mounts (toluidine blue, DIC) of cercaria (D) and metacercaria (E). (F) Anterior end of metacercaria with gland ducts, CLSM, acetylated α-tubulin and phospho Y antibody staining. (G) Sagittal section of metacercaria, Heidenhain's iron hematoxylin staining. (H) Sagittal section of metacercaria, Mallory's trichrome stain. (I) progenetic metacercaria (toluidine blue, DIC). (J) sexual adult (acetic carmine, DIC). Scale bars – 50 μm.Abbreviations: aс – anterior collecting duct; bp – birth pore canal; c – ceca; cd – caudal excretory duct; ci – cirrus; eg – eggs; ev – excretory vesicle; os – oral sucker; ot – ootype; ov – ovary; ovd – oviduct; pс – posterior collecting duct; pe – pigmented eyespots; pd – penetration gland ducts; ph – pharynx; sv – seminal vesicle; t – tail; te – testes; vi – vitelline follicles; vs – ventral sucker. Arrow indicate on site of main collecting duct division.. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Fig. 1 in Life cycle truncation in Digenea, a case study of Neophasis spp. (Acanthocolpidae)

Fig. 1. Consensus (99 threshold) neighbour-joining tree based on the concatenated ITS1 and ITS2 860- b.p. fragment, built with Tamura-Nei genetic distance method and 1000 bootstrap resamples; support values are printed at nodes. Repeat regions of the ITS1 were excluded from the alignment. Scale bar shows substitutions per site. The ingroup includes identified and putative life cycle stages of Neophasis oculata and N. anarrhichae, the numbers of isolates are as listed in Table 1. Brachycladium goliath serves as an outgroup.

Fig. 5 in Fish blood flukes (Digenea: Aporocotylidae) from Indonesia: Two new genera and species infecting the banded eagle ray, Aetomylaeus nichofii (Bloch and Schneider, 1801) Capape´and Desoutter, 1979 (Myliobatiformes: Myliobatidae) from Borneo

Fig. 5. Phylogenetic relationships of chondrichthyan blood flukes based on morphological characters (tegumental spines, shape of intestines). Host affiliations are included. Dashed lines indicate species with no nucleotide sequences. Boxes indicate spine rows: blue = 2 + spine rows, green = 1 spine row, and red ⋂ = no spines. Shape of the intestine () inverse U-shaped and (X) X-shaped. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Fig. 1-2. Aetohemecus kirstenjensenae Warren and Bullard n. gen., n in Fish blood flukes (Digenea: Aporocotylidae) from Indonesia: Two new genera and species infecting the banded eagle ray, Aetomylaeus nichofii (Bloch and Schneider, 1801) Capape´and Desoutter, 1979 (Myliobatiformes: Myliobatidae) from Borneo

Fig. 1-2. Aetohemecus kirstenjensenae Warren and Bullard n. gen., n. sp. (Digenea: Aporocotylidae) from the heart of the banded eagle ray, Aetomylaeus nichofii (Bloch and Schneider, 1801) Capape´and Desoutter, 1979 (Myliobatiformes: Myliobatidae). (1) Body of holotype (USNM No. 1642775), dorsal view. Bar = 250 μm. (2) Genitalia, paratype (USNM No. 1642776), ventral view. Bar = 100 μm. Mouth (mo), nerve commissure (nc), oesophagus (os), vitellarium (vit), intestine (i), testis (t), uterus (u), metraterm (met), ovary (o), vas deferens (v), seminal vesicle (sv), cirrus sac (cs), cirrus (c), vitelline duct (vd), common genital pore (cgp), oviducal ampullae (oa), and o¨otype (oo).

Fig. 3-4. Homestios janinecairae Warren and Bullard n. gen., n in Fish blood flukes (Digenea: Aporocotylidae) from Indonesia: Two new genera and species infecting the banded eagle ray, Aetomylaeus nichofii (Bloch and Schneider, 1801) Capape´and Desoutter, 1979 (Myliobatiformes: Myliobatidae) from Borneo

Fig. 3-4. Homestios janinecairae Warren and Bullard n. gen., n. sp. (Digenea: Aporocotylidae) from the heart of the banded eagle ray, Aetomylaeus nichofii (Bloch and Schneider, 1801) Capape´and Desoutter, 1979 (Myliobatiformes: Myliobatidae). (3) Body of holotype (USNM No. 1642774), dorsal view. Bar = 250 μm. (4) Genitalia of holotype (USNM No. 1642774), dorsal view. Bar = 100 μm. Mouth (mo), oesophagus (os), vitellarium (vit), intestine (in), testis (t), ovary (ov), vas deferens (vd), uterus, (u), ascending uterus (au), descending uterus (du), seminal vesicle (sv), cirrus (c), and common genital pore (cgp).