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Fig. 4 in Three new species of Eimeria (Apicomplexa: Eimeriidae) from the Amami rabbit, Pentalagus furnessi (Mammalia: Leporidae)

Fig. 4. Nomarski interference contrast photographs of oocysts of Eimeria hilleri from the Amami rabbit stored in a potassium dichromate solution. M: micropyle; N: nucleus; OWo: outer layer of the wall; OWi: inner layer of the wall; RB: refractile body; SB: Stieda body; SR: sporocyst residuum; SSB: substieda body. Scale bars (all in the same scale) = 10 μm.

Fig. 2 in Three new species of Eimeria (Apicomplexa: Eimeriidae) from the Amami rabbit, Pentalagus furnessi (Mammalia: Leporidae)

Fig. 2. Endogenous stages of coccidian parasites found in the section of the jejunum and ileum of the Amami rabbit. HE. Scale bar = 20 μm and applies to all parts. (a) Schizont-like structures (arrows) in detached epithelial cells. (b) Immature microgamont (arrow) with basophilic small nuclei. (c) Immature macrogamont (arrow) with eosinophilic bodies. (d) Microgamont (arrow) with peripherally located basophilic nuclei. (e) Macrogamont (arrow) with peripheral arrangement of eosinophilic wall-forming bodies. (f) Unsporulated oocyst (arrow) with a central nucleus and oocyst wall. High-resolution version of these slides for use with the Virtual Microscope are available as eSlide: VM06653.

Fig. 5 in Three new species of Eimeria (Apicomplexa: Eimeriidae) from the Amami rabbit, Pentalagus furnessi (Mammalia: Leporidae)

Fig. 5. Nomarski interference contrast photographs of oocysts of Eimeria sagentae from the Amami rabbit stored in a potassium dichromate solution. M: micropyle; N: nucleus; OWo: outer layer of the wall; OWi: inner layer of the wall; RB: refractile body; SB: Stieda body; SO: sporont; SR: sporocyst residuum. Scale bars (all in the same scale) = 10 μm.

Fig. 1 in Three new species of Eimeria (Apicomplexa: Eimeriidae) from the Amami rabbit, Pentalagus furnessi (Mammalia: Leporidae)

Fig. 1. Map showing the location of the Amami-Oshima and Tokunoshima Islands. Circles indicate the sites where fecal samples of Amami rabbits were collected in the field.

Fig. 3 in Three new species of Eimeria (Apicomplexa: Eimeriidae) from the Amami rabbit, Pentalagus furnessi (Mammalia: Leporidae)

Fig. 3. Nomarski interference contrast photographs of oocysts of Eimeria furnessi from the Amami rabbit stored in a potassium dichromate solution. M: micropyle; N: nucleus; OWo: outer layer of the wall; OWi: inner layer of the wall; RB: refractile body; SB: Stieda body; SO: sporont; SR: sporocyst residuum. Scale bars (all in the same scale) = 10 μm.

opencc-by-4.0Apr 2024View details →

Fig. 3 in Detection of Sarcocystis albifronsi, Eimeria alpacae, and Cystoisospora felis in Eurasian lynx (Lynx lynx) in northwestern China

Fig. 3. Phylogenetic tree based on partial gene of the 18S rRNA sequence of Cystoisospora felis (▴) from Eurasian lynx cub #3 obtained in this study in northwestern China. The evolutionary history was inferred using the neighbor-joining method (bootstrap replicates: 1000) with MEGA 7.0.

opencc-by-4.0Apr 2024View details →

Fig. 2 in Detection of Sarcocystis albifronsi, Eimeria alpacae, and Cystoisospora felis in Eurasian lynx (Lynx lynx) in northwestern China

Fig. 2. Phylogenetic tree based on partial gene of the COI sequence of Eimeria alpacae (▴) from Eurasian lynx #2 obtained in this study in northwestern China. The evolutionary history was inferred using the neighbor-joining method (bootstrap replicates: 1000) with MEGA 7.0.

opencc-by-4.0Apr 2024View details →

Fig. 1 in Detection of Sarcocystis albifronsi, Eimeria alpacae, and Cystoisospora felis in Eurasian lynx (Lynx lynx) in northwestern China

Fig. 1. Phylogenetic tree based on partial gene of the COI sequence of Sarcocystis albifronsi (▴) from Eurasian lynx #2 obtained in this study in northwestern China. The evolutionary history was inferred using the neighbor-joining method (bootstrap replicates: 1000) with MEGA 7.0.

Fig. 2 in Remarks on Eimeria spp. (Apicomplexa: Eimeriidae) from Kobus spp. (Bovidae: Reduncini), with supplementary morphological data of Eimeria congolensis Ricci-Bitti et al., 1973 from a new host subspecies, the common waterbuck Kobus ellipsiprymnus ellipsiprymnus (Ogilbyi, 1833)

Fig. 2. Photomicrographs of sporulated oocysts of Eimeria congolensis from common waterbucks Kobus ellipsiprymnus ellipsiprymnus in a safari park of Portugal. Note the inner layer (il) and rough outer layer (rol) of the oocyst wall, micropyle (m), nucleous (n), polar granule (pg), refractile body (rb), sporocyst residuum (sr), Stieda (sb) and sub-stieda (ssb) bodies. Scale bar: 10 μm.

Fig. 1 in Remarks on Eimeria spp. (Apicomplexa: Eimeriidae) from Kobus spp. (Bovidae: Reduncini), with supplementary morphological data of Eimeria congolensis Ricci-Bitti et al., 1973 from a new host subspecies, the common waterbuck Kobus ellipsiprymnus ellipsiprymnus (Ogilbyi, 1833)

Fig. 1. Composite line drawing of the sporulated oocyst of Eimeria congolensis from common waterbucks Kobus ellipsiprymnus ellipsiprymnus in a safari park of Portugal. Scale-bar: 10 μm.

Fig. 3 in Molecular evaluation of Eimeria spp. Infection in the Volga-Ural Saiga antelope population of the Republic of Kazakhstan

Fig. 3. Phylogenetic tree generated by the Maximum Composite Likelihood (MCL) method using partial sequences of the 18S rRNA of the Eimeria species that infect the Saiga tatarica.

Fig. 2 in Molecular evaluation of Eimeria spp. Infection in the Volga-Ural Saiga antelope population of the Republic of Kazakhstan

Fig. 2. Eimeria spp. Oocyst at 40x magnification. The outer (OL) and the inner (IL) layers of the oocyst wall, micropyle (M), micropyle cap (MC), spores (S).

Fig. 1 in Molecular evaluation of Eimeria spp. Infection in the Volga-Ural Saiga antelope population of the Republic of Kazakhstan

Fig. 1. Photomicroscope images showing the oocysts of E. elegans (40х magnification). The outer (OL) and the inner (IL) layers of the oocyst wall, cytoplasmic mass (CM), micropyle (M).

opencc-by-4.0Jul 2023View details →

Data, scripts, and figures of the article: Evaluation of oregano essential oil in broilers challenged with a mixed Eimeria spp. and high dietary protein model of subclinical coccidiosis

Data, scripts, and figures of the article "Evaluation of oregano essential oil in broilers challenged with a mixed Eimeria spp. and high dietary protein model of subclinical coccidiosis" to be published in the journal Animal - Open Space.

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Reduction of chickens use to perform in vitro pre-screening of novel anticoccidials by miniaturisation and increased throughput of the current Eimeria tenella compound-screening model

In vitro models have supported important advances in biomedical sciences and have significantly contributed to reduce the use of experimental animals in different disciplines. We have developed an in vitro model for the evaluation of potential anticoccidial properties of novel compounds aimed to control chicken coccidiosis, a costly disease for the poultry industry. This disease is caused by protozoan parasites of the genus Eimeria (Apicomplexa), and it is mainly controlled by chemoprophylaxis with ionophors and chemical anticoccidials; however, there is an overall agreement about the limitation of these classical drugs and the need to improve current methods of control. Anticoccidial activities of novel compounds is currently evaluated by expensive experiments that involve large numbers of chickens. The use of our in vitro model for the pre-screening of essential oils led to a reduction of 67% of the chickens used in the vivo trials for validation. In this study, we describe how further optimisation of this in vitro model by miniaturisation can have an additional impact on the number of chickens used for the generation of parasite stocks for provision of the in vitro model (which cannot be done in vitro). We have estimated that the use of one chicken could support the evaluation of ten compounds with a 96-well plate format vs. only two with a 24-well plate format, which means an 80% of chicken use reduction. In this study, we have proved that the miniaturisation into a 96-well plate format has perfectly mimicked the invasion and replication observed before in the 24-well plate format. In addition, this format has allowed the simultaneous pre-screening of higher numbers of anticoccidial drugs at different concentrations following streamlined protocols in a more cost-effective way, factors that are beneficial for a wider uptake of the model by other researchers investigating anticoccidial compounds.

opencc-zeroSep 2022View details →

opencc-by-4.0May 2024View details →

Figure 1 in Quantitative phosphoproteomic analysis of chicken DF-1 cells infected with Eimeria tenella, using tandem mass tag (TMT) and parallel reaction monitoring (PRM) mass spectrometry

Figure 1. Proportion of serine, threonine, and tyrosine in phosphorylation sites.

opencc-by-4.0Sep 2024View details →

Multi-omics analysis reveals regime shifts in the gastrointestinal ecosystem in chickens following anticoccidial vaccination and Eimeria tenella challenge

A multi-omics study integrating gut microbiota and host metabolome to investigate the gastrointestinal health markers in broiler chickens (Cobb500) from an anti-coccidiosis vaccine trial.

opencc-by-4.0Aug 2019View details →

Database of Eimeria species of ruminants in Mexico

This database contains the occurrences of Eimeria species that infect cattle, sheep and goats in Mexico. Data includes records from 1961 to 2018.

opencc-by-4.0May 2021View details →

Table 1 in Molecular characterization and protective efficacy of a new conserved hypothetical protein of Eimeria tenella

Table 1. Protective effect of r Et CHP protein on E. tenella infection.<table><tbody><tr><th>Group</th><th>Average body</th><th>Mean lesion scores</th><th>Oocyst shedding</th><th>Percentage reduction</th></tr></tbody><tbody><tr><th></th><td>weight gains (g)</td><td></td><td>per bird (× 10 7)</td><td>of oocyst excretion (%)</td></tr><tr><th>Unchallenged control</th><td>258.62 ± 70.26c</td><td>0.00 ± 0.00a</td><td>0.00 ± 0.00a</td><td>100d</td></tr><tr><th>Challenged control</th><td>180.87 ± 45.38a</td><td>3.20 ± 0.83c</td><td>4.43 ± 0.99c</td><td>0.00a</td></tr><tr><th>r Et CHP-50 μ g</th><td>226.25 ± 24.47b</td><td>1.75 ± 0.95b</td><td>2.10 ± 0.88b</td><td>54.07 ± 11.76c</td></tr><tr><th>r Et CHP-100 μ g</th><td>235.25 ± 23.44bc</td><td>1.20 ± 0.44b</td><td>3.26 ± 2.47bc</td><td>31.99 ± 29.35b</td></tr></tbody></table>a–d Values with different letters in the same column are significantly different (P <0.05) according to the ANOVA Duncan test.