Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
126
datasets available to search
ShareScore release 0.9.0
Dataset results
126 results for “Extraction Method”
Figure 1 from: Hudz N, Yezerska O, Shanaida M, Horčinová Sedláčková V, Wieczorek PP (2019) Application of the Folin-Ciocalteu method to the evaluation of Salvia sclarea extracts. Pharmacia 66(4): 209-215. https://doi.org/10.3897/pharmacia.66.e38976
Figure 1 Dependence of absorbance on wavelength for the interaction of gallic acid (100 µg/L) with the FCR (after 60 min of reaction time).
Figure 2 from: Hudz N, Yezerska O, Shanaida M, Horčinová Sedláčková V, Wieczorek PP (2019) Application of the Folin-Ciocalteu method to the evaluation of Salvia sclarea extracts. Pharmacia 66(4): 209-215. https://doi.org/10.3897/pharmacia.66.e38976
Figure 2 Dependence of absorbance on wavelength for E-4 of Salvia sclarea (dilution 1:10) with the FCR (after 60 min of reaction time).
Data from: DNA extraction method affects the detection of a fungal pathogen in formalin-fixed specimens using qPCR
Museum collections provide indispensable repositories for obtaining information about the historical presence of disease in wildlife populations. The pathogenic amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) has played a significant role in global amphibian declines, and examining preserved specimens for Bd can improve our understanding of its emergence and spread. Quantitative PCR (qPCR) enables Bd detection with minimal disturbance to amphibian skin and is significantly more sensitive to detecting Bd than histology; therefore, developing effective qPCR methodologies for detecting Bd DNA in formalin-fixed specimens can provide an efficient and effective approach to examining historical Bd emergence and prevalence. Techniques for detecting Bd in museum specimens have not been evaluated for their effectiveness in control specimens that mimic the conditions of animals most likely to be encountered in museums, including those with low pathogen loads. We used American bullfrogs (Lithobates catesbeianus) of known infection status to evaluate the success of qPCR to detect Bd in formalin-fixed specimens after three years of ethanol storage. Our objectives were to compare the most commonly used DNA extraction method for Bd (PrepMan, PM) to Macherey-Nagel DNA FFPE (MN), test optimizations for Bd detection with PM, and provide recommendations for maximizing Bd detection. We found that successful detection is relatively high (80–90%) when Bd loads before formalin fixation are high, regardless of the extraction method used; however, at lower infection levels, detection probabilities were significantly reduced. The MN DNA extraction method increased Bd detection by as much as 50% at moderate infection levels. Our results indicate that, for animals characterized by lower pathogen loads (i.e., those most commonly encountered in museum collections), current methods may underestimate the proportion of Bd-infected amphibians. Those extracting DNA from archived museum specimens should ensure that the techniques they are using are known to provide high-quality throughput DNA for later analysis.
Data from: More than skin and bones: comparing extraction methods and alternative sources of DNA from avian museum specimens
Next-generation sequencing has greatly expanded the utility and value of museum collections by revealing specimens as genomic resources. As the field of museum genomics grows, so does the need for extraction methods that maximize DNA yields. For avian museum specimens, the established method of extracting DNA from toe pads works well for most specimens. However, for some specimens, especially those of birds that are very small or very large, toe pads can be a poor source of DNA. In this study, we apply two DNA extraction methods (phenol-chloroform and silica column) to three different sources of DNA (toe pad, skin punch, and bone) from ten historical avian museum specimens. We show that a modified phenol-chloroform protocol yielded significantly more DNA than a silica column protocol (e.g., Qiagen DNeasy Blood & Tissue Kit) across all tissue types. However, extractions using the silica column protocol contained longer fragments on average than those using the phenol-chloroform protocol, likely a result of loss of small fragments through the silica column. While toe pads yielded more DNA than skin punches and bone fragments, skin punches proved to be a reliable alternative source of DNA and might be especially appealing when toe pad extractions are impractical. Overall, we found that historical bird museum specimens contain substantial amounts of DNA for genomic studies under most extraction scenarios, but that a phenol-chloroform protocol consistently provides the high quantities of DNA required for most current genomic protocols.
Motivações para Aplicação da Refatoração Extract Method: Um Estudo Baseado em Mensagens de Commit
Open the record for dataset details and reuse information.
Supplementary material 1 from: Bernardes SC, von Rintelen T, Alexander S, Lorenz F, von Rintelen K (2023) Assessing 'non-destructive' DNA extraction method in small crustaceans kept in wet collections. Research Ideas and Outcomes 9: e113299. https://doi.org/10.3897/rio.9.e113299
Supplementary Table S1
Supplementary material 3 from: Bernardes SC, von Rintelen T, Alexander S, Lorenz F, von Rintelen K (2023) Assessing 'non-destructive' DNA extraction method in small crustaceans kept in wet collections. Research Ideas and Outcomes 9: e113299. https://doi.org/10.3897/rio.9.e113299
Supplementary Figure S2
Supplementary material 2 from: Bernardes SC, von Rintelen T, Alexander S, Lorenz F, von Rintelen K (2023) Assessing 'non-destructive' DNA extraction method in small crustaceans kept in wet collections. Research Ideas and Outcomes 9: e113299. https://doi.org/10.3897/rio.9.e113299
Supplementary Figure S1
Figure 2 from: Strus O, Fedorovska M, Holota S, Polovko N (2021) Development and validation of standardization methods of aqueous sapropel extract. Pharmacia 68(4): 933-939. https://doi.org/10.3897/pharmacia.68.e71783
Figure 2 The dependence of the concentration of the total mass fraction of HA on the mass of the ASE sample.
Figure 1 from: Strus O, Fedorovska M, Holota S, Polovko N (2021) Development and validation of standardization methods of aqueous sapropel extract. Pharmacia 68(4): 933-939. https://doi.org/10.3897/pharmacia.68.e71783
Figure 1 Structures of humic acid basic core (A) and fulvic acid (B). Source: (Wang and Mulligan 2018).
Supplementary material 2 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Final species table. :
Supplementary material 5 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Methods (Scripts) :
Supplementary material 3 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Number of reads per sample. :
Supplementary material 4 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Diversity analyses. :
Supplementary material 1 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Fragment and working PCR conditions. :
Supplementary material 5 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
Alternative statistical model :
Supplementary material 4 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
4_NTC :
Supplementary material 2 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
Bioinformatic pipeline and thresholds :
Supplementary material 3 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
Profiling tables for all libraries :
Supplementary material 1 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
Extraction protocols :
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.