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Figure 2 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 2 Species of Ramalina on rocky seashores in Italy/Sardinia A general view of species and habitat B from left to right: R. tingitana, R. breviuscula and R. cribrosaCR. implexaD from left to right: R. clementeana and R. requieniiER. tingitanaFR. inaequalis. Photographs by M. Guissard and E. Sérusiaux.

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Figure 9 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 9 Evolutionary tree for the genera Namibialina and Ramalina (subset of Matrix 1). The tree is a close-up of Figure 6. Values above branches represent the posterior probabilities of support. Table on right side provides further information for all accessions: column 1-4: ß-depsidones (protocetraric acid, salazinic acid, stictic acid, norstictic acid); column 5-8: depsides (divaricatic acid, sekikaic acid, evernic acid, lecanoric acid); column 9: triterpenes; column 10: bourgeanic acid; column 11: unknown fatty acid; greyish colour through columns 1-11 for two accessions (R. complanata and R. crinita) means that no data are available.

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Supplementary material 4 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Table S4. Time calibration

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Figure 4 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 4 Several species of RamalinaAR. hoehneliana, hanging down the branches of a large Strombosia scheffleri in Gishwati forest (Rwanda) BR. sinensis (Armenia) CR. azorica (Azores, Pico) DR. huei (Canary Is., Tenerife) ER. nodosa (Canary Is., Tenerife). Photographs by E. Sérusiaux.

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Figure 7 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Figure 7 Evolutionary tree for the genera Niebla and Vermilacinia, produced with the 6-locus matrix (Matrix 2) and using RAxML. Support value for branches follow Lemoine et al. (2018). Epithets in colour following insert: green = collected in USA/California; pink = collected in Mexico/Baja California; blue = collected in Mexico/Baja California Sur. Table on right side provides further information for all accessions: column 1-3: ß-depsidones (protocetraric acid, salazinic acid, hypoprotocetraric acid); column 4-5: depsides (divaricatic acid, sekikaic acid); column 6-11: [-]-16α-hydroxykaurane, triterpenes T1, T2, T3, unidentified triterpenes, zeorin; column 12-13: fatty acid, usnic acid; greyish colour through columns 1-13 for one accession (V. cephalota) means that no data are available; column 14–17: results of species delimitation methods: 14 = ABGD; 15 = PTP on 1 locus; 16 = BPP; 17 = STACEY.

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Supplementary material 5 from: Spjut R, Simon A, Guissard M, Magain N, Sérusiaux E (2020) The fruticose genera in the Ramalinaceae (Ascomycota, Lecanoromycetes): their diversity and evolutionary history. MycoKeys 73: 1-68. https://doi.org/10.3897/mycokeys.73.47287

Table S5. Comparison of the identification of the Niebla collections

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Supplementary material 3 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Specimens of Loxospora chloropolia, L. elatina and L. ochrophaea revised for this study

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Figure 8 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Figure 8 Morphology of Loxospora elatina (for details of specimens, see Table 1, Suppl. material 3) A, B thalli with tuberculate areoles and irregular and partly fused soralia (A UGDA L-47757 B UGDA L- 47762) C thallus with soralia bursting from areoles and later fused (UGDA L-47761) D soralia covering most parts of the thallus (UGDA L-47760) E, F apothecia with sorediate or esorediate margins (O L-97759). Scale bars: 1 mm.

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Figure 7 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Figure 7 Morphology of Loxospora chloropolia (for details of specimens, see Table 1, Suppl. material 3) A−C smooth to folded thalli with mostly discrete soralia (A UGDA L-60095 B UGDA L-31983 C UGDA L-54253) D, E thalli with folded to areolate areas (D UGDA L-60093 E UGDA L-60096) F apothecia with sorediate margins (Ellis L456, E 01043201). Scale bars: 1 mm.

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Figure 6 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Figure 6 Morphology of two species of ChicitaeaA Thallus of Ch. confusa on tree trunk (taken by J. Hollinger in the field) B thallus of Ch. cristinae on tree trunk (taken by D. Kubiak in the field) C, D Thalli of Ch. cristinae showing soralia (paratypes of L. cristinaeC UGDA L-22396 D UGDA L-20385). Scale bars: 1 mm (C, D).

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Supplementary material 2 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Sequences obtained from GenBank and used in phylogenetic analyses

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Figure 2 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Figure 2 Haplotype network showing relationships between nuITS rDNA sequences from Loxospora chloropolia, L. elatina and L. ochrophaea. The names of species are followed with sample numbers (see Table 1, Suppl. material 2). Newly-sequenced samples are marked in bold. Mutational changes are presented as numbers in brackets near lines between haplotypes.

opencc-by-4.0Feb 2024View details →
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Figure 4 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Figure 4 Haplotype network showing relationships between mtSSU rDNA sequences from Chicitaea assateaguensis, Ch. confusa and Ch. lecanoriformis. The names of species are followed with sample numbers (see Table 1, Suppl. material 2). Newly-sequenced samples are marked in bold. Mutational changes are presented as numbers in brackets near lines between haplotypes.

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Figure 1 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Figure 1 IQ-tree based on a combined nuITS rDNA, mtSSU and RPB1 dataset for Loxospora s.l. The names of species are followed with sample number (see Table 1, Suppl. material 2). Bootstrap supports from IQ-tree analysis ≥ 70 (first value) and posterior probabilities from BA ≥ 0.95 (second value) are indicated near the branches. Umbilicaria spp. were used as outgroup. Loxospora chloropolia clade is marked with blue box and Chicitaea gen. nov. is marked with green box.

opencc-by-4.0Feb 2024View details →
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Figure 3 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Figure 3 Haplotype network showing relationships between RPB1 sequences from Loxospora chloropolia, L. elatina and L. ochrophaea. The names of species are followed with sample numbers (see Table 1, Suppl. material 2). Newly-sequenced samples are marked in bold. Mutational changes are presented as numbers in brackets near lines between haplotypes.

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Supplementary material 1 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Conditions for each set of primers used in PCR

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Figure 5 from: Ptach-Styn Ł, Guzow-Krzemińska B, Lendemer JC, Tønsberg T, Kukwa M (2024) Phylogeny of the genus Loxospora s.l. (Sarrameanales, Lecanoromycetes, Ascomycota), with Chicitaea gen. nov. and five new combinations in Chicitaea and Loxospora. MycoKeys 102: 155-181. https://doi.org/10.3897/mycokeys.102.116196

Figure 5 Haplotype network showing relationships between nuITS rDNA sequences from Chicitaea assateaguensis and Ch. confusa. The names of species are followed with sample numbers (see Table 1, Suppl. material 2). Newly-sequenced samples are marked in bold. Mutational changes are presented as numbers in brackets near lines between haplotypes.

opencc-by-4.0Feb 2024View details →
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Figure 8 from: Xie C-M, Wang L-S, Zhao Z-T, Zhang Y-Y, Wang X-Y, Zhang L-L (2022) Revision of Immersaria and a new lecanorine genus in Lecideaceae (lichenised Ascomycota, Lecanoromycetes). MycoKeys 87: 99-132. https://doi.org/10.3897/mycokeys.87.72614

Figure 8 Lecaimmeria mongolica (a–d SDNU20190354): a–b thallus c apothecial anatomy d ascospores. Scale bars: 1 mm (a–b); 20 μm (c); 10 μm (d).

opencc-by-4.0Feb 2022View details →
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Figure 1 from: Xie C-M, Wang L-S, Zhao Z-T, Zhang Y-Y, Wang X-Y, Zhang L-L (2022) Revision of Immersaria and a new lecanorine genus in Lecideaceae (lichenised Ascomycota, Lecanoromycetes). MycoKeys 87: 99-132. https://doi.org/10.3897/mycokeys.87.72614

Figure 1 Phylogenetic tree constructed from Maximum Likelihood analyses in Lecideaceae, based on the concatenated nrITS-nrLSU-RPB1-RPB2-mtSSU dataset. Maximum Likelihood bootstrap probabilities above 70% (left) and Bayesian Inference posterior probabilities above 0.9 (right) are given at the nodes.

opencc-by-4.0Feb 2022View details →
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Figure 5 from: Xie C-M, Wang L-S, Zhao Z-T, Zhang Y-Y, Wang X-Y, Zhang L-L (2022) Revision of Immersaria and a new lecanorine genus in Lecideaceae (lichenised Ascomycota, Lecanoromycetes). MycoKeys 87: 99-132. https://doi.org/10.3897/mycokeys.87.72614

Figure 5 Immersaria ferruginea (a–cKUN 20–69144): a–b thallus c apothecial anatomy. I. shangrilaensis (d–fKUN 18–60430): d–e thallus f apothecial anatomy. Scale bars: 1 mm (a–b, d–e); 20 μm (c, f).

opencc-by-4.0Feb 2022View details →

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