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Fig. 1 in Polyclinum constellatum (Tunicata, Ascidiacea), an emerging non-indigenous species of the Mediterranean Sea: integrated taxonomy and the importance of reliable DNA barcode data Abstract

Fig. 1: Map of the Mediterranean Sea showing the literature records (black rhombuses) of P. constellatum and the new findings (red dots). performed in a final reaction volume of 25 μl contain- nus was reconstructed with the online software PHYML ing: 1X reaction buffer with 1 mM final concentration of v3.0 (http://www.atgc-montpellier.fr/phyml-sms/) (Guin- MgCl 2 (Takara Bio Inc.), 0.2 mM of each dNTP, 0.3 μM don & Gascuel, 2003), which also includes the automatof each primer and 1.25 Units of PrimeStar HS (Takara ic model selection algorithm SMS (Smart Model Selec- Bio Inc.). Amplification conditions were: 30 cycles with tion). The best-fit substitution model was selected using denaturation for 10 s at 98°C, annealing for 15 s at 46°C the Akaike Information Criterion (AIC). Bootstrap val- or 50°C, extension for 1 min 30 s at 72°C; a final elonga- ues, indicating node reliability, were based on 100 reption step of 5 min at 72°C. licates. The sequence dataset used for this phylogenetic PCRs with the DreamTaq polymerase were performed reconstruction is reported in Supplementary Table S1 and in a final volume of 25 μl containing: 1X reaction buffer was extracted from the phylogenetic dataset published in with 2 mM final concentration of MgCl 2 (Thermo Fish- Tabudravu et al. (2019). It includes representative species er Scientific), 0.2 mM of each dNTP, 0.4 μM of each of of the Polyclinidae family plus Eudistoma and Pseudodithe two primers, and 1.25 Units of DreamTaq polymerase stoma species chosen as outgroups for their morphologi- (Thermo Fisher Scientific). The amplification conditions cal similarities with Polyclinidae. were as follows: an initial denaturation for 3 min at 95°C, then 34 amplification cycles (denaturation for 30 s at 95°C; annealing for 30 s at 46-50°C; extension for 1 min Results 30 s at 72°C) followed by a final elongation step of 5 min at 72°C. Morphological analyses The obtained amplicons were purified with the DNA Clean&Concentrator kit (Zymo Research) and directly The colonies collected in Taranto harbour and Hersequenced according to the Sanger method by Microsynth aklion marina were all morphologically identified as P. AG (Switzerland). The sequence quality check, compar- constellatum based on the following features: colonies isons and alignment were carried out with Geneious ver. without sand in/outside, zooids arranged in systems, 5.5.7.2 (Kearse et al., 2012). The sequences obtained post-abdomen (without vascular stolon) shorter than the were deposited in the GenBank database (see Accession thorax and abdomen combined, pharynx with 16-18 rows numbers MT873559 and OL597608). For comparative of stigmata, more than 15 stigmata per row, and a 6-lobed analyses, homologous sequences of the genus Polycli- anus. These characteristics are in accordance with the key num were searched for in the non-redundant nucleotide of Polyclinum species edited by Kott (1963) and they are database (nr-nt db, on 21st September 2021) of the NCBI also reported in the description of the species made by (National Center for Biotechnology Information) by En- Van Name (1945). trez text search, and by BLASTn (Altschul et al., 1990) using our P. constellatum sequences as the query. Uncorrected pairwise distances were calculated with PAUP 4.0a (Swofford, 2002), while a Maximum Likelihood (ML) phylogenetic tree of the genus Polyclinum ge-

opencc-by-4.0Feb 2022View details →
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Protect Your Edmonton Property: Reliable Sump Pump Installation Solutions

<p>To avoid flooding in the basement, it is a good idea to install an&nbsp;<a href="https://shieldfoundationrepair.nicepage.io/blog/benefits-of-edmonton-sump-pump-installation-you-didnt-know-about.html"><strong>Edmonton sump pump</strong></a>. This will effectively remove any excess water from the foundation. The annoyance and expense of dealing with a flooded basement are things that homeowners may save themselves by doing this. A sump pump can also help keep the house structurally sound by reducing the likelihood of mildew and mould growth. In the case of heavy rain or runoff, the sump pump will ensure that the basement remains dry and secure, which is a relief.</p> <p>If your basement floods every time it rains in the spring or if you've noticed standing water near your home's foundation, a sump pump may be necessary. When water collects in low-lying areas, such as a basement or crawl space, a sump pump may remove it from the foundation and dispose of it properly by directing it to the sewer system. If you're still on the fence about whether or not a sump pump is a good investment for your home, consider these benefits.</p> <p>Installing a sump pump is a smart move any time of year, but especially during times of heavy rain when flooding is a real possibility. The absence of action will allow mould and mildew to flourish in these areas of standing water. Mildew and mould can degrade indoor air quality and lead to costly structural damage to your home.</p> <p>Finally, there are several&nbsp;benefits to having a sump pump in Edmonton that extend beyond just preventing basement flooding. The structural integrity and value of Edmonton real estate are greatly enhanced by sump pumps, which shield properties from water damage, mildew, and mould. Sump pumps also improve interior air quality, which is good for the health of building inhabitants.</p> <p>Shield Foundation Repair, Inc. has finally arrived! For more information about our sump pump solutions, please visit our website or call us at 780-760-4900. If you have any questions about installing a sump pump, how much it will cost, or what benefits it offers, we are here to help with any plumbing problem you may be having.</p> <h1>In most cases, how much does it cost to install a sump pump?</h1> <p>To&nbsp;protect your basement or other lower-level floor from flooding and water damage, a sump pump should be installed. Among its components are water-level or pressure-sensing valves and a motor unit that may transfer water from one spot to another, all within a reasonable distance from your home. A man-made trench is the usual location for subterranean sump pumps.</p> <p>The average cost to install a sump pump can be high, especially if you're not familiar with the area or are doing it for the first time. Sump pump installation services are usually rather cheap, but the cost quickly escalates when you include in the cost of a professional installer or&nbsp;if you want to do it yourself, any extra parts you'll need to buy.</p> <p><a href="https://www.shieldfoundationrepair.ca/services/drainage-installation-sump-pumps/"><strong>Edmonton sump pump installation</strong></a>&nbsp;costs can vary widely depending on factors including the type of pump, how challenging the installation is, and whether or not any other features or accessories are included. Think about the long-term benefits of a sump pump and how it can protect your home from water damage and structural issues. The upfront cost may be steep, but the investment will be more than compensated for by the security it provides and the absence of worry about floods. Homeowners can make better-informed&nbsp;decisions about sump pump installations when they know what to expect in terms of price.</p> <p>For the most part, when people think of a sump pump, they imagine the pump and motor as being one unit. Just as its name implies, a submersible sump pump is immersed inside the basin to save space and minimize noise. They should be completely resistant to corrosion and water, as their purpose is to endure substantial amounts of water. The submersion characteristic reduces the lifespan of these devices compared to pedestal pumps. As an added downside, they are more difficult to access for repairs or maintenance. This page lists the unit and installation costs for submersible sump pumps.</p> <p>It is highly recommended that you use a professional for sump pump installation rather than attempting the task on your own. A costly and inefficient ordeal awaits in the event of improper installation. Therefore, dial the number of Shield Foundation Repair Inc. immediately. If you are experiencing problems with your sump pump, we are happy to help you find the right solution.</p> <h2>A Homeowner's Guide to Understanding and Fixing Foundation Cracks</h2> <p>An indication that something went wrong is the presence of foundation cracks. Your priority should be to identify the type of foundation cracks you're dealing with. One effective way to repair cracks in foundations is with epoxy injection. This technique involves pouring epoxy into the cracks to seal them and form a firm bond that prevents further water entry. Hydraulic cement patching is an alternative method that uses a fast-setting cement mixture to successfully cover larger foundation cracks and holes.</p> <p>The total stability of the foundation can be enhanced by reinforcing the repaired areas with carbon fibre. To determine the best option for their unique situation, homeowners should be knowledgeable of these strategies and consider consulting an expert. The following are signs of possible structural cracks: stair-like fissures in brick or concrete block walls.</p> <ul> <li>fissures that extend horizontally throughout the framework of your home.</li> <li>straight vertical cracks arranged in a parallelogram.</li> <li>wide, diagonal cracks along the length of your walls.</li> <li>cracks along the length of your walls and even into your ceiling.</li> </ul> <p>Do you want to know what causes foundation cracks outside and how to fix them? If so, you've found the right spot, as it is precisely the topic that this essay will discuss. Among other things, we'll go over methods for avoiding foundation cracks and fixing them externally.</p> <h2>Get the Insider Knowledge on Sump Pumps from Local Experts</h2> <p>Regular inspections of your sump pump for signs of wear or malfunction, such as unusual vibrations or noises, are recommended by Shield Foundation Repair Inc. Clearing the discharge pipe of debris and ensuring it extends away from the foundation are further steps to take to prevent water from seeping back into the basement. Last but not least, protect your home from any flood damage by having a professional inspect and service your sump pump once a year.</p> <p>A sump pump is a very simple piece of equipment. I can confirm that it is a pump. If your home is ever flooded, the water will inevitably find its way to the sump pump, which is located at the lowest point on your land. The sump pit is where the pump is housed; in smaller pits, it may be placed on a pedestal, and in larger ones, it may be positioned below the water line. When the water level reaches a certain point, a float switch is activated, turning on the pump. After the water level drops low enough, the pump will keep sucking it out of the area.</p> <p>Once in a while, to get the pump going, fill the sump pit with a bucket. Here we have a simple functionality test.<br>Plan your maintenance. Unless you own a whole-house generator, you probably won't put this item to frequent use. Do not put off finding problems till an unfavourable time comes.<br>Consider bringing an extra battery just in case. If the power goes out, this backup pump will keep running&mdash;which is likely to happen in situations when a sump pump is needed.</p> <p>If you want to know more about sump pumps and anything else relating to home comfort, go over to Shieldfoundationrepair.com right now. Save money on services and more by taking advantage of our exclusive promotions!</p>

opencc-by-4.0Jun 2024View details →
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Fig. 1 in Polyclinum constellatum (Tunicata, Ascidiacea), an emerging non-indigenous species of the Mediterranean Sea: integrated taxonomy and the importance of reliable DNA barcode data Abstract

Fig. 1: Map of the Mediterranean Sea showing the literature records (black rhombuses) of P. constellatum and the new findings (red dots).

opencc-by-4.0Feb 2022View details →
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FIGURE 4 in HOW DO WE IDENTIFY MICRONYCTERIS (SCHIZONYCETRIS) SANBORNI SIMMONS, 1996 (CHIROPTERA, PHYLLOSTOMIDAE) RELIABLY AND WHERE WE CAN FIND THIS SPECIES IN BRAZIL?

FIGURE 4: Diagnostic cranial traits for the differentiation of M. minuta (UFPB 8695) and M. sanborni (A, B, C: UFPB 6543, D and E: UFPB 6535). White arrow: A = P3; B = anterior portion of the zygomatic arch; C = Dorsal profile of skull; D = p; E = articular 3 process. See Table 2.

opencc-by-4.0Dec 2015View details →
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FIGURE 2 in HOW DO WE IDENTIFY MICRONYCTERIS (SCHIZONYCETRIS) SANBORNI SIMMONS, 1996 (CHIROPTERA, PHYLLOSTOMIDAE) RELIABLY AND WHERE WE CAN FIND THIS SPECIES IN BRAZIL?

FIGURE 2: Occlusal view of the upper dentition of M. sanborni (upper: UFPB 6536; middle: UFPB 6734; lower: UFPB 6793). The insets to the right highlight differences in the spacing of p3 and p4 and the variation in the development of the posterolingual cingulum of p4 (white arrow).

opencc-by-4.0Dec 2015View details →
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FIGURE 3 in HOW DO WE IDENTIFY MICRONYCTERIS (SCHIZONYCETRIS) SANBORNI SIMMONS, 1996 (CHIROPTERA, PHYLLOSTOMIDAE) RELIABLY AND WHERE WE CAN FIND THIS SPECIES IN BRAZIL?

FIGURE 3: Differences in fur color between M. sanborni e M. minuta. A: Pale gray ventral fur of Micronycteris minuta and pure white ventral fur of Micronycteris sanborni. B: Short white basal dorsal hair in M. minuta and long white basal dorsal hair in M. sanborni.

opencc-by-4.0Dec 2015View details →
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FIGURE 5 in HOW DO WE IDENTIFY MICRONYCTERIS (SCHIZONYCETRIS) SANBORNI SIMMONS, 1996 (CHIROPTERA, PHYLLOSTOMIDAE) RELIABLY AND WHERE WE CAN FIND THIS SPECIES IN BRAZIL?

FIGURE 5: Recorded localities for Micronycteris sanborni in relation to Brazilian biomes. Circles: previous records; Triangles: new records. The sites are numbered as in Table 3. "?" refers to a doubtful records

opencc-by-4.0Dec 2015View details →
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FIGURE 1 in HOW DO WE IDENTIFY MICRONYCTERIS (SCHIZONYCETRIS) SANBORNI SIMMONS, 1996 (CHIROPTERA, PHYLLOSTOMIDAE) RELIABLY AND WHERE WE CAN FIND THIS SPECIES IN BRAZIL?

FIGURE 1: Oblique frontal view of the upper dentition in M. sanborni (A: UFPB 6793; B: UFPB 6795; C: UFPB 6536) and M. minuta (D: UFPB 8695). Note the variation in the gap between the outer upper incisor and the canine (white arrow).

opencc-by-4.0Dec 2015View details →
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Рис. 1. Имаго, общий виΔ: 1–3 — Orthosia ariuna, Хабаровск; 4–6 — O. incerta, Хабаровск. 1, 2, 4, 5 — самцы; 3, 6 — самки. Àаты сбора: 1 — 19.04.2020; 2 — 9.05.2020; 3, 4 — 18.04.2020; 5 — 13.04.2020; 6 — 16.04.2020 Fig. 1. Adults, habitus: 1–3 — Orthosia ariuna, Khabarovsk; 4–6 — O. incerta, Khabarovsk. 1, 2, 4, 5 — males; 3, 6 – females. Collection dates: 1 — 19.04.2020; 2 — 9.05.2020; 3, 4 — 18.04.2020; 5 — 13.04.2020; 6 — 16.04.2020 in The First Reliable Record Of A Little-Known Species Orthosia Ariuna Hreblay, 1991 (Lepidoptera, Noctuidae) From The Far East Of Russia

Рис. 1. Имаго, общий виΔ: 1–3 — Orthosia ariuna, Хабаровск; 4–6 — O. incerta, Хабаровск. 1, 2, 4, 5 — самцы; 3, 6 — самки. Àаты сбора: 1 — 19.04.2020; 2 — 9.05.2020; 3, 4 — 18.04.2020; 5 — 13.04.2020; 6 — 16.04.2020 Fig. 1. Adults, habitus: 1–3 — Orthosia ariuna, Khabarovsk; 4–6 — O. incerta, Khabarovsk. 1, 2, 4, 5 — males; 3, 6 – females. Collection dates: 1 — 19.04.2020; 2 — 9.05.2020; 3, 4 — 18.04.2020; 5 — 13.04.2020; 6 — 16.04.2020

opencc-by-4.0Dec 2020View details →
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Figure 1 in First reliable record of many-plumed moth Pterotopteryx spilodesma (Lepidoptera, Alucitidae) on Sakhalin Island

Figure 1. Pterotopteryx spilodesma (Meyrick, 1908), female. Left – adult specimen; right – genitalia (scale bars: 1mm).

opencc-by-4.0Feb 2020View details →
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A Meta-Analysis on the Reliability of Comparative Judgement Data

<p>This is the data and R analysis script with the article &quot;A Meta-Analysis on the Reliability of Comparative Judgement&quot;</p>

opencc-by-4.0Mar 2018View details →
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Extended data for the paper "Reliable generation of native-like decoys limits predictive ability in fragment-based protein structure prediction"

<p>Extended data for the paper:<br> Reliable generation of native-like decoys limits predictive ability in fragment-based protein structure prediction</p> <p>Authors:<br> Shaun M Kandathil, Mario Garza-Fabre, Simon C Lovell and Julia Handl</p> <p>--------------------------------</p> <p>Contents of the zip file:</p> <p>&nbsp;</p> <p>Directory &#39;ECDFplots&#39;:<br> ----------------------<br> &nbsp;&nbsp; &nbsp;Data corresponding to Figure 3 for all targets, for the bilevel and ILS protocols. Data are available following stages 3 and 4 of the low-resolution protocol.</p> <p>Directory &#39;ScoreRMSDplots_3archivers&#39;:<br> --------------------------------------<br> &nbsp;&nbsp; &nbsp;Data corresponding to Figures 6 and 9 for all targets. Data corresponding to decoys obtained after low-resolution stages 3 and 4 can be found in subdirectories &#39;Stage3&#39; and &#39;Stage4&#39;, respectively.<br> &nbsp;</p>

opencc-by-4.0Jul 2019View details →
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Figures 2–6 in A reliable and efficient BioPulverizer method in preparing and grinding nematodes for nucleic acid extraction and molecular identification

Figures 2–6 PCR amplification product with primers: (2) PCR products from the seminested primer pairs (NemF and 18Sr2b; NF1 and 18Sr2b) obtained from soil nematode samples with BioPulverizer grinding; (3) PCR amplification with the first cycle of the primer pair (NemF and 18Sr2b) from soil samples without BioPulverizer grinding; (4–5) Two amplification bands, 181 bp with the species-specific primer pair GlyF1/rDNA2 (4) and 477 bp with SCNF1/SCNR1 (5), were amplified for Heterodera glycines; (6) PCR products with the universal primer pair 194F/195R and the species-specific primer pair (Meloidogyne incognita) from potato tuber samples. All molecular markers (M) are 100 bp DNA ladders. The concentrations of agarose gel are 1.8% in Figs 2–3 and 1% in Figs 4–6.

opencc-by-4.0Dec 2023View details →
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Figure 1 in A reliable and efficient BioPulverizer method in preparing and grinding nematodes for nucleic acid extraction and molecular identification

Figure 1 Equipment used for nematode preparation and grinding. The names of all the equipment are listed above or under each respective one.

opencc-by-4.0Dec 2023View details →
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Figure 4 in CHD genes: a reliable marker for bird populations and phylogenetic analysis? Case study of the superfamily Sylvioidea (Aves: Passeriformes)

Figure 4. Maximum clade credibility trees for three data sets obtained by BI analysis in BEAST v1.8.0. MCC genealogy based on a Yule speciation process, summarized from the output of a Markov chain Monte Carlo chain run for 10 million iterations and sampled every 1000 iterations.

opencc-by-4.0Apr 2016View details →
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Figure 3 in CHD genes: a reliable marker for bird populations and phylogenetic analysis? Case study of the superfamily Sylvioidea (Aves: Passeriformes)

Figure 3. The ML tree for myoglobin gene (in blue) and for CHD-Z gene (in red); both trees are scaled in substitutions and the length of each tree and different topologies indicate that both of them are improper for phylogeny using a single gene.

opencc-by-4.0Apr 2016View details →
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Figure 1. Sex distribution for A. arundinaceus, A in CHD genes: a reliable marker for bird populations and phylogenetic analysis? Case study of the superfamily Sylvioidea (Aves: Passeriformes)

Figure 1. Sex distribution for A. arundinaceus, A. scirpaceus, and A. schoenobaenus, shown by sampling date.

opencc-by-4.0Apr 2016View details →
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Figure 5 in A reliable method for quick comparisons of enchytraeid (Oligochaeta) densities in soil and their seasonal changes under cultivated and natural fields in central Greece

Figure 5. Principal component analysis of the samples collected from two sites, alfalfa field and boundary zone based on nine soil properties.

opencc-by-4.0Jul 2024View details →
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Figure 4 in A reliable method for quick comparisons of enchytraeid (Oligochaeta) densities in soil and their seasonal changes under cultivated and natural fields in central Greece

Figure 4. Seasonal changes in total precipitation and mean monthly temperature in Kopaida valley during the period April 2021 – March 2022.

opencc-by-4.0Jul 2024View details →
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Figure 3 in A reliable method for quick comparisons of enchytraeid (Oligochaeta) densities in soil and their seasonal changes under cultivated and natural fields in central Greece

Figure 3 depicts the monthly changes of the mean soil moisture of all three soil depths and the instant soil temperature at the sampling time at 10 cm depth. It is obvious that these two parameters altered identically in the two fields and only small differences can be detected, e.g. the rise in soil moisture in July in the alfalfa field due to the application of irrigation water.

opencc-by-4.0Jul 2024View details →

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Allen Brain Atlas

Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.

allen-brain-atlas
neuroscienceopenDocumentation, web resources, and API references are available online.
Last verified 2026-04-30Open record

Annotated Behaviour and Observability Dataset (ABODe)

ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.

abode-home-cage
behavioral-neuroscienceopenThe DataShare record exposes download links for annotations, documentation, license text, and the zipped per-snippet data directory.
Last verified 2026-04-30Open record

DANDI Archive for NWB datasets

DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.

dandi-nwb
electrophysiologyopenPublished Dandiset metadata and archive endpoints are available through the production DANDI API.
Last verified 2026-04-30Open record

International Brain Laboratory public data

The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.

ibl
behavioral-neuroscienceopenPublic sessions can be searched and loaded from the IBL public data server through ONE.
Last verified 2026-04-29Open record

OpenNeuro

OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.

openneuro
neuroscienceopenPublished datasets are available on demand over the internet.
Last verified 2026-04-29Open record