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209 results for “confocal microscopy”
FIGURE 6 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 6. Eriocheir sinensis, ZI, first maxilliped, CLSM images with Drishti processing. (A) whole appendage, (B) coxa and basis, (C) coxa and basis rotated to reveal reverse angle of image A, (D) endopod, (E) exopod with 4 natatory setae. Objective: B-D = 40× oil immersion; A, E = 20× dry. Scale bars A, B, D = 100 µm; C, E = 200 µm.
FIGURE 16 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 16. Eriocheir sinensis, ZIII, CLSM images with Drishti processing. (A) anterior view of carapace, applying "large images" option with a scanned area of 2×3 fields for image stitching, (B) ventral carapace margin, (C) dorsal spine with 1 pair of setae, applying "large images" option with a scanned area of 1×2 fields for image stitching. Objective: 20× dry. Scale bars = 200 µm.
FIGURE 13 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 13. Eriocheir sinensis, ZII, first maxilliped, CLSM images with Drishti processing. (A) whole appendage, applying "large images" option with a scanned area of 1×2 fields for image stitching, (B) endopod, (C) basis, (D) exopod with 6 natatory setae, applying "large images" option with a scanned area of 1×2 fields for image stitching. Objective: A, D = 20× dry; B-C = 40× oil immersion. Scale bars A, D = 200 µm; B-C = 100 µm.
FIGURE 15 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 15. Eriocheir sinensis, ZII, pleon and telson, CLSM images with Drishti processing. (A) dorsal view of pleon, image merged using Adobe Photoshop, (B) lateral view of pleon and telson, applying "large images" option with a scanned area of 1×2 fields for image stitching, (C) pleomere 1 with a small dorsal medial seta, (D) dorsal view of telson. Objective: A, C = 40× oil immersion; B, D = 20× dry. Scale bars = 200 µm.
FIGURE 23 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 23. Eriocheir sinensis, ZIV, CLSM images with Drishti processing. (A) anterior view of carapace, (B) dorsal spine with 2 pairs of setae, applying "large images" option with a scanned area of 1×3 fields for image stitching. Objective: 20× dry. Scale bars = 200 µm.
FIGURE 11 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 11. Eriocheir sinensis, ZII, CLSM images with Drishti processing. (A) antennule, image merged using Adobe Photoshop, (B) antenna with two exopodal setae, (C) maxillule. Objective: 40× oil immersion. Scale bars A–B = 50 µm; C = 100 µm.
FIGURE 18 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 18. Eriocheir sinensis, ZIII, CLSM images with Drishti processing. (A) mandible, (B) maxillule, applying "large images" option with a scanned area of 1×2 fields for image stitching. Objective: 40× oil immersion. Scale bars = 100 µm.
FIGURE 7 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 7. Eriocheir sinensis, ZI, second maxilliped, CLSM images with Drishti processing. (A) whole appendage, (B) coxa, basis and endopod, applying "large images" option with a scanned area of 1×2 fields for image stitching, (C) distal endopod article. Objective: A = 20× dry; B = 40× oil immersion; C = 60× oil immersion. Scale bars A-B = 100 µm; C = 50 µm.
FIGURE 20 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 20. Eriocheir sinensis, ZIII, first maxilliped, CLSM images with Drishti processing. (A) coxa and basis, image merged using VGStudio MAX, (B) exopod with 8 natatory setae, applying "large images" option with a scanned area of 1×2 fields for image stitching, (C) endopod, image merged using VGStudio MAX. Objective: A, C = 40× oil immersion; B = 20× dry. Scale bars A, C = 100 µm; B = 200 µm.
Confocal microscopy image stacks from "Temporal integration of auxin information for the regulation of patterning"
<p>This dataset contains raw images in CZI format (Zeiss) of shoot apical meristems (SAM) from <em>Arabidopsis thaliana </em>transgenic lines <strong>qDII-pCLV3-pDR5</strong> or <strong>qDII-pCLV3-PIN1</strong>. See <em>(Galvan-Ampudia and Cerutti et al.) </em>for detailed information. This data constitutes the input of the <strong>sam_spaghetti</strong> pipeline (<a href="https://gitlab.inria.fr/mosaic/publications/sam_spaghetti">https://gitlab.inria.fr/mosaic/publications/sam_spaghetti</a>) and can be processed using the scripts and examples provided in the package.</p> <p> </p> <p><strong>File information:</strong></p> <p>File names containing qDII-CLV3-DR5 have the following data:</p> <ul> <li>Channel 1: <em>DII-VENUS-N7</em></li> <li>Channel 2: <em>pDR5:2xmTurquoise2</em></li> <li>Channel 3: <em>pRPS5a:TagBFP-SV40</em></li> <li>Channel 4: <em>pCLV3:mCherry-N7</em></li> </ul> <p>File names containing qDII-CLV3-PIN1-PI have the following data:</p> <ul> <li>Channel 1: <em>DII-VENUS-N7</em></li> <li>Channel 2: <em>pPIN1:PIN1-GFP</em></li> <li>Channel 3: <em>Propidium Iodide (cell walls)</em></li> <li>Channel 4: <em>pRPS5a:TagBFP-SV40</em></li> <li>Channel 5: <em>pCLV3:mCherry-N7</em></li> </ul> <p>Time-lapse sequences are identified as follows:</p> <ul> <li><strong>qDII-CLV3-DR5-E27-LD-SAM7.czi</strong></li> <li><strong>qDII-CLV3-DR5-E27-LD-SAM7-T5.czi</strong></li> <li><strong>qDII-CLV3-DR5-E27-LD-SAM7-T10.czi</strong></li> </ul> <p>where:</p> <ul> <li><strong>qDII-CLV3-DR5</strong> indicates the line</li> <li><strong>E$$-LD</strong> (e.g. E25-LD, E27-LD, etc) indicates independent biological replicas</li> <li><strong>SAM$</strong> is the meristem (technical replica)</li> <li><strong>T$</strong> indicates the time elapsed after the first image (in hours)</li> </ul> <p>For example <strong>qDII-CLV3-DR5-E27-LD-SAM7-T5.czi</strong> is an image of the 7th SAM of the set E27, acquired 5 hours after the first image.</p>
FIGURE 11 in Confocal microscopy applied to water mite taxonomy with the description of a new genus of Axonopsinae (Acari, Parasitengona, Hydrachnidia) from Central America
FIGURE 11. Bright field microscopy (left) and confocal (right) EDF images built with set of 100 consecutive optical slices. Step between optical slices 0.2 µm.
FIGURES 1–4. Vagabundia sci n in Confocal microscopy applied to water mite taxonomy with the description of a new genus of Axonopsinae (Acari, Parasitengona, Hydrachnidia) from Central America
FIGURES 1–4. Vagabundia sci n. sp. (holotype). 1, ventral shield, CLSM; 2, dorsal shield, CLSM; 3, fourth leg EDF with bright field microscopy; 4, fourth leg EDF with confocal slicing.
FIGURES 5–10. Vagabundia sci n in Confocal microscopy applied to water mite taxonomy with the description of a new genus of Axonopsinae (Acari, Parasitengona, Hydrachnidia) from Central America
FIGURES 5–10. Vagabundia sci n. sp. (holotype). 5, dorsal shield; 6, ventral shield; 7, II-Leg 2-6; 8, IV-Leg 2-6; 9, capitulum, lateral view; 10, right palp, lateral view.
FIGURE 8 in Oziella sibirica (Acari: Eriophyoidea: Phytoptidae), a new eriophyoid mite species described using confocal microscopy, COI barcoding and 3 D surface reconstruction
FIGURE 8. CLSM image of the internal genitalia of Oziella sibirica sp. nov. A. Spermatheca; B. Pre-spermathecal swelling (distal segment of spermathecal tube); C. Proximal segment of spermathecal tube; D. Longitudinal bridge; E. Transverse apodeme; F. Laterodistal fold of transversal apodeme.
FIGURE 6 in Oziella sibirica (Acari: Eriophyoidea: Phytoptidae), a new eriophyoid mite species described using confocal microscopy, COI barcoding and 3 D surface reconstruction
FIGURE 6. Oziella sibirica sp. nov., nymph. A. Dorsal view of the mite; B. Ventral view; C. Prodorsal shield; D. Coxigenital area; E. Right leg I (arrow indicates a spine); F. Right leg II; G. Typical 4/3-rayed empodium; H. Typical 4/4-rayed empodium; I. Abnormal empodium. Scale bar: A & B = 140; C & D = 50; E & F = 45; G, H & I = 12.
FIGURE 1. 3D in Oziella sibirica (Acari: Eriophyoidea: Phytoptidae), a new eriophyoid mite species described using confocal microscopy, COI barcoding and 3 D surface reconstruction
FIGURE 1. 3D model of Oziella sibirica sp. nov. prodorsal shield (the same female as Fig. 2C). A. Gray scale prodorsal shield, B. Colourised prodorsal shield (notifications of lines follows that of Amrine et al. 1994 & Amrine et al. 2003; admedian lines colourised in red, additional line between admedian and submedian-2 line colourised in black). Scale bar A & B = 30 mkm. Note: setae ve and c1 are short on images A & B because only proximal parts of the setae of eriophyoid mites can be observed on CLSM images using blue laser, 405 nm (Chetverikov 2012b).
FIGURE 2 in Oziella sibirica (Acari: Eriophyoidea: Phytoptidae), a new eriophyoid mite species described using confocal microscopy, COI barcoding and 3 D surface reconstruction
FIGURE 2. Variation of the prodorsal shield design among four females of Oziella sibirica sp. nov. (black & white inverted CLSM images). Admedian lines colourised in red (A & D), additional lines in green (B) and red (C; the same female as in Fig. 2A & 2B). Scale bar = 30 mkm.
FIGURE 4 in Oziella sibirica (Acari: Eriophyoidea: Phytoptidae), a new eriophyoid mite species described using confocal microscopy, COI barcoding and 3 D surface reconstruction
FIGURE 4. Microphotographs under light phase contrast microscopy (A & B) and CLSM (C & D) of Oziella sibirica sp. nov. A. Coxigenital region; B. Female prodorsal shield (arrows indicate the eye-like structures); C. Position and view of internal genitalia inside female; D. Epiandrium on male venter. Scale bar A, B, C, D = 20 mkm.
FIGURE 9 in Oziella sibirica (Acari: Eriophyoidea: Phytoptidae), a new eriophyoid mite species described using confocal microscopy, COI barcoding and 3 D surface reconstruction
FIGURE 9. Morphometrics of the internal genitalia of Oziella sibirica sp. nov. Length (A–B) and width (C–D) of spermatheca; length (E–F) and width (G–H) of prespermathecal swelling (distal segment of spermathecal tube); length (I–J) of proximal segment of spermathecal tube; length (J–K) of longitudinal bridge; half-length (K–L) of genital apodeme; maximal distance between left and right parts of transversal apodeme (L–M).
FIGURE 3 in Oziella sibirica (Acari: Eriophyoidea: Phytoptidae), a new eriophyoid mite species described using confocal microscopy, COI barcoding and 3 D surface reconstruction
FIGURE 3. Oziella sibirica sp. nov., female. A. Ventral view of the mite, B. Coxigenital area, C. Female prodorsal shield; D. Female internal genitalia, E. Empodium; F. Epiandrium, G. Leg I (arrow indicates a spine), H. Leg II. Scale bar: A = 130; B & C = 45; D = 35; E = 15; F = 45; G & H = 35.
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Allen Brain Atlas
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Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
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