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Figure 4 in A phylogeographical perspective on the ex situ conservation of Aylacostoma (Thiaridae, Gastropoda) from the High Paraná River (Argentina-Paraguay)

Figure 4. Maximum-likelihood tree of Aylacostoma specimens based on 658 nucleotides of the partial cytochrome oxidase subunit I gene and the spatial distribution of geographical populations in the High Paraná River. Bootstrap values are shown above and below the branches. Numbers within clades are GenBank accession numbers. References to localities are given in Table 1.

Figure 5 in A phylogeographical perspective on the ex situ conservation of Aylacostoma (Thiaridae, Gastropoda) from the High Paraná River (Argentina-Paraguay)

Figure 5. Divergence times for Aylacostoma from the High Paraná River. The nodes in the phylogram are presented according to the mean value of oldest estimated age, assuming different divergence rates and substitution models. Divergence between A. chloroticum and A. brunneum = 3.60 Mya (K2P); divergence within A. chloroticum = 0.38 Mya (GTR+I+G). The bars around each node represent the minimum and maximum estimated age. Time scale adapted from the International Chronostratigraphic Chart (International Commission on Stratigraphy, 2014).

Figure 3 in A phylogeographical perspective on the ex situ conservation of Aylacostoma (Thiaridae, Gastropoda) from the High Paraná River (Argentina-Paraguay)

Figure 3. Median-joining network depicting all haplotypes found for the Aylacostoma specimens from the High Paraná River included in the ex situ conservation programme. The size of circles is proportional to haplotype frequency. Mutational steps separating haplotypes are indicated. For localities, see Table 1.

Figure 2 in A phylogeographical perspective on the ex situ conservation of Aylacostoma (Thiaridae, Gastropoda) from the High Paraná River (Argentina-Paraguay)

Figure 2. Evolution of the old port of Candelaria city (Misiones, Argentina; 27°26′50.96″S, 55°45′0.84″W). This is the only location along the High Paraná River where a wild population of Aylacostoma chloroticum is still known to occur after completing the filling of the Yacyretá Reservoir in 2011. Note the drastic modification of the environment between 2003 and November 2011, with the construction of an artificial beach for recreational use. The white arrow highlights the increase in water level between August and December 2010.

Figure 1 in A phylogeographical perspective on the ex situ conservation of Aylacostoma (Thiaridae, Gastropoda) from the High Paraná River (Argentina-Paraguay)

Figure 1. Living specimens of Aylacostoma born in captivity within the ex situ conservation programme. A, Aylacostoma chloroticum (note the juvenile on the adult specimen). B, Aylacostoma brunneum.

opencc-by-4.0Dec 2022View details →

Data to reproduce the results presented in de Lange et al. 2023. Water Resources Research, "The Impact Of Flocculation on In Situ and Ex Situ Particle Size Measurements by Laser Diffraction"

This repository consists data to reproduce results as presented in: "The Impact Of Flocculation on In Situ and Ex Situ Particle Size Measurements by Laser Diffraction", Water Resources Research. Kindly refer to the readme.text file to navigate through the dataset.

opennotspecifiedMar 2022View details →

FIGURE. Blades of C. spongifolia. A. Sub-marginal and marginal collective veins, with laminal tissue (2–4 mm) between. B. Marginal collective veins fused below shallow sinus. C. Underside with spongy appearance produced by "false pores"; and thick, rubbery texture indicated by axial wrinkles formed in crease. D. Underside showing primary vein and pinnate lateral veins surrounded by spongy tissue. E. Sub-stomatal cavities revealed by transmitted light (scale unit 0.25 mm), F. Sub-stomatal cavities revealed by removing lower epidermis (A–D: Mengla County, 2018. E–F: Bach Ma NP seedling, ex situ). Photos: PJM. in Colocasia spongifolia sp. nov. (Araceae) in southern China and central Vietnam

FIGURE. Blades of C. spongifolia. A. Sub-marginal and marginal collective veins, with laminal tissue (2–4 mm) between. B. Marginal collective veins fused below shallow sinus. C. Underside with spongy appearance produced by "false pores"; and thick, rubbery texture indicated by axial wrinkles formed in crease. D. Underside showing primary vein and pinnate lateral veins surrounded by spongy tissue. E. Sub-stomatal cavities revealed by transmitted light (scale unit 0.25 mm), F. Sub-stomatal cavities revealed by removing lower epidermis (A–D: Mengla County, 2018. E–F: Bach Ma NP seedling, ex situ). Photos: PJM.

opencc-by-nc-4.0Sep 2022View details →

Data from: Continuous inbreeding affects genetic variation, phenology and reproductive strategy in ex situ cultivated Digitalis lutea

Data from several experiments studying population differences between a wild and a botanic garden population and effects of different hand-pollination treatments on various traits of the short-lived perennial plant Digitalis lutea. Separate spreadsheets contain data on 1) genetic variation using ISSR markers, 2) effects of pollination treatments on seed number and seed mass, 3) differences in germination, 4) various traits studied in a common garden experiment, 5) effects of population and pollination treatments on pollinator visitation. See publication in American Journal of Botany (2022) for details. Abstract: Premise: Ex situ cultivation is important for plant conservation, but cultivation in small populations may result in genetic changes by drift, inbreeding or unconscious selection. Repeated inbreeding potentially influences not only plant fitness, but also floral traits and interactions with pollinators, which has not yet been studied in an ex situ context. Methods: We studied the molecular genetic variation of Digitalis lutea L. from a botanic garden population cultivated for 30 years, a frozen seed bank conserving the original genetic structure, and two current wild populations including the source population. In a common garden we studied the effects of experimental inbreeding and between-population crosses on performance, reproductive traits and flower visitation of plants from the garden and a wild population. Results: Significant genetic differentiation was found between the garden population and the wild population from which the seeds had originally been gathered. After experimental selfing, inbreeding depression was only found in germination and leaf size of plants from the wild population, indicating a history of inbreeding in the smaller garden population. Moreover, garden plants flowered earlier and showed floral traits related to selfing, whereas wild plants showed traits related to attracting pollinators. Bumblebees visited more flowers of outbred than inbred plants and of wild than garden plants. Conclusions: Our case study suggests that high levels of inbreeding during ex situ cultivation can influence reproductive traits and thus interactions with pollinators. Together with the effects of genetic erosion and unconscious selection this may affect the success of reintroductions into natural habitats.

opennotspecifiedMar 2017View details →

Fig. 3 in Ex-situ cultivation at lower altitude and evaluation of Swertia chirayita, a critically endangered medicinal plant of Sikkim Himalayan region, India

Fig. 3. An image of HPTLC plate at UV 254 nm showing swertiamarin content, track 1: standard swertiamarin, track 2: swertiamarin content in stem (niche environment), track 3: swertiamarin content in leaves (niche environment), track 4: swertiamarin content in leaves (ex-situ cultivated plant) (a); HPTLC densitogram of swertiamarin standard (b), stem (niche environment) (c), leaves (niche environment) (d) and leaves (ex-situ cultivated plant) (e), [x-axis represent retention factor (Rf) & y-axis represent absorption unit (AU)].

opennotspecifiedMar 2017View details →

Fig. 1 in Ex-situ cultivation at lower altitude and evaluation of Swertia chirayita, a critically endangered medicinal plant of Sikkim Himalayan region, India

Fig. 1. Fully mature crop cultivated in niche environment (a), fully mature dried harvested plants from niche environment (b), one-year-old ex-situ cultivated plants at lower altitude (c), freshly harvested leaves from one-year-old ex-situ cultivated plants (d) and leaf: sessile leaves of fully mature plant cultivated in niche environment (left) and petiolated leaves of oneyear-old ex-situ cultivated plants (right) (e).

opencc-zeroDec 2014View details →

Data from: Founded: genetic reconstruction of lineage diversity and kinship informs ex situ conservation of Cuban Amazon parrots (Amazona leucocephala)

Captive breeding is a widespread conservation strategy, yet such programs rarely include empirical genetic data for assessing management assumptions and meeting conservation goals. Cuban Amazon parrots (Amazona leucocephala) are considered vulnerable, and multiple on-island captive populations have been established from wild-caught and confiscated individuals of unknown ancestry. Here, we used mitochondrial haplotypic and nuclear genotypic data at 9 microsatellite loci to quantify the extent and distribution of genetic variation within and among captive populations in Zapata Swamp and Managua, Cuba, and to estimate kinship among breeders (n = 88). Using Bayesian clustering analysis, we detected 2 distinct clusters within the Zapata population, one of which was shared with Managua. Individuals from the cluster unique to Zapata possessed mitochondrial haplotypes with affinities to Cuban subspecies (A. l. leucocephala, A. l. palmarum); the shared cluster was similar, but also included haplotypes closely related to the subspecies restricted to Cayman Brac (A. l. hesterna). Overall mean kinship was low within each captive population (−0.026 to −0.012), with 19 and 11 recommended breeding pairs in Zapata and Managua, respectively, ranked according to mean kinship and informed by molecular sexing. Our results highlight the importance of understanding population history within ex situ management programs, while providing genetic information to directly inform Cuban parrot conservation.

opencc-zeroDec 2013View details →

Data from: Ex situ conservation of underutilised fruit tree species: establishment of a core collection for Ficus carica L. using microsatellite markers (SSRs)

Ex situ germ plasm collections of woody crops are necessary to ensure the optimal use of plant genetic resources. The fig tree (Ficus carica L.) germ plasm bank, consisting of 229 accessions, is located in Centro de Investigación 'La Orden'. Despite great progress in conservation, ex situ collections face size and organization problems. Core collections obtained from structured samples of bigger collections are a useful tool to improve germ plasm management. In this work, we used simple sequence repeat (SSR) markers to establish a core collection in this underutilised Mediterranean fruit tree species. Four approaches have been carried out (random sampling, maximization, simulated annealing and stepwise clustering) to determine the best method to develop a core collection in this woody plant. The genetic diversity obtained with each subset was compared with that of the complete collection. It was found that the most efficient way to achieve the maximum diversity was the maximization strategy, which, with 30 accessions, recovers all the SSR alleles and does not show significant differences in allele frequency distribution in any of the loci or in the variability parameters (H O, H E) between the whole and core collections. Thus, this core collection, a representative of most fig diversity conserved in the germ plasm bank, could be used as a basis for plant material exchange among researchers and breeders.

opencc-zeroDec 2020View details →

Genetic diversity and population structure of two endangered neotropical parrots inform In Situ and Ex Situ conservation strategies

A key aspect in the conservation of endangered populations is understanding patterns of genetic variation and structure, which can provide managers with critical information to support evidence-based status assessments and management strategies. This is especially important for species with small wild and larger captive populations, as found in many endangered parrots. We used genotypic data to assess genetic variation and structure in wild and captive populations of two endangered parrots, the blue-throated macaw, Ara glaucogularis, of Bolivia, and the thick-billed parrot, Rhynchopsitta pachyrhyncha, of Mexico. In the blue-throated macaw, we found evidence of weak genetic differentiation between wild northern and southern subpopulations, and between wild and captive populations. In the thick-billed parrot we found no signal of differentiation between the Madera and Tutuaca breeding colonies or between wild and captive populations. Similar levels of genetic diversity were detected in the wild and captive populations of both species, with private alleles detected in captivity in both, and in the wild in the thick-billed parrot. We found genetic signatures of a bottleneck in the northern blue-throated macaw subpopulation, but no such signal was identified in any other subpopulation of either species. Our results suggest both species could potentially benefit from reintroduction of genetic variation found in captivity, and emphasize the need for genetic management of captive populations.

ClinicalTrials.gov32/100

Efficacy of Ex-situ Normothermic Perfusion Versus Cold Storage in the Transplant With Steatotic Liver Graft.

ClinicalTrials.gov study NCT03930459. IPD Sharing: Not stated. Countries: 1. Publications: 13.

restrictedIPD-UNDECIDEDFeb 2026View details →

ClinicalTrials.gov32/100

Synchrotron Imaging of Human Ovaries Ex Situ

ClinicalTrials.gov study NCT01631786. IPD Sharing: Not stated. Countries: 1. Publications: 1.

restrictedIPD-UNDECIDEDFeb 2026View details →