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97 results for “laser scanning microscopy”
Figure 4 in Confocal laser scanning microscopy analysis of the phalloidin-labelled musculature in nemerteans
Figure 4. Confocal projections of the longitudinal (A–H, J) and transversal (I) sections of anterior proboscis region. (A, B) Inner circular musculature of Carinoma mutabilis (A) and Lineus alborostratus (B); (C) longitudinal, diagonal and outer circular musculature of Carinina sp.; (D) anterior proboscis bulb of Carinomella sp.; (E, F) interweaving of the longitudinal musculature of Micrura kulikovae (E) and Malacobdella grossa (F); (G, H, J) longitudinal and diagonal musculature of Cephalothrix sp. (G), Carinoma mutabilis (H), and Micrura kulikovae (J); (I) everted proboscis of Carinoma mutabilis (muscle crosses indicated by arrows; high magnification image shows muscle cross). Abbreviations: dm, diagonal muscles; icm, inner circular musculature; lm, longitudinal musculature; ocm, outer circular musculature. Scales: 50 µm.
Figure 1 in Confocal laser scanning microscopy analysis of the phalloidin-labelled musculature in nemerteans
Figure 1. Confocal projections of the longitudinal (A–G) and transversal (H, I) sections of the body wall musculature. (A) Epidermal muscle meshwork immediately posterior to the mouth in Carinoma mutabilis, ventral view; (B) diagonal epidermal muscle fibres (arrows), pigment ring region of Callinera sp., dorsal view; (C) radial epidermal muscles (arrows) of Carinina sp., anterior region, ventral view of body margin; (D–F) intraganglionic radial muscles (arrows) in the brain of Carinoma mutabilis (D), in the lateral nerve cords of Cephalothrix cf. simula (E), and in the head of Cephalothrix sp., larva (F), all dorsal view; (G–I) Cerebratulus marginatus, radial epidermal muscles, just behind mouth, ventral view (G), fragment of transversal section through the intestine region showing epidermal muscles (arrows) (H), fragment of transversal section through the foregut region showing radial muscles (arrows) (I). Abbreviations: br, brain; dm, dermal muscles; dvm, dorsoventral muscles; fg, foregut; ep, epidermis; ilm, inner longitudinal musculature; lm, longitudinal musculature; ln, longitudinal nerve cord; mcm, middle circular musculature; pr, proboscis. Scales: 50 µm.
Data from: Spatiotemporal rank filtering improves image quality compared to frame averaging in 2-photon laser scanning microscopy
Live imaging of biological specimens using optical microscopy is limited by tradeoffs between spatial and temporal resolution, depth into intact samples, and phototoxicity. Two-photon laser scanning microscopy (2P-LSM), the gold standard for imaging turbid samples in vivo, has conventionally constructed images with sufficient signal-to-noise ratio (SNR) generated by sequential raster scans of the focal plane and temporal integration of the collected signals. Here, we describe spatiotemporal rank filtering, a nonlinear alternative to temporal integration, which makes more efficient use of collected photons by selectively reducing noise in 2P-LSM images during acquisition. This results in much higher SNR while preserving image edges and fine details. Practically, this allows for at least a four fold decrease in collection times, a substantial improvement for time-course imaging in biological systems.
Data from: Spatiotemporal rank filtering improves image quality compared to frame averaging in 2-photon laser scanning microscopy
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Multiphoton Laser Scanning Microscopy in Cutaneous Optical Pathology
ClinicalTrials.gov study NCT00508781. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Intraoperative Confocal Laser Scanning Microscopy With Use of AI for Optimized Surgical Excision of Basal Cell Carcinoma
ClinicalTrials.gov study NCT06600165. IPD Sharing: NO. Countries: 1. Publications: 0.
In Vivo Confocal Scanning Laser Microscopy of Benign Nevi
ClinicalTrials.gov study NCT00487864. IPD Sharing: Not stated. Countries: 1. Publications: 0.
FIGURE 48 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 48. Eriocheir sinensis, ZVI, maxilla, CLSM images with Drishti processing. (A) whole appendage, applying "large images" option with a scanned area of 3×3 fields for image stitching, 1 seta on exopod circled and arrowed, (B) maxilla rotated to show from reverse angle of image A and the setation of the coxal and basial endites. Objective: 40× oil immersion. Scale bars = 200 µm.
FIGURE 46 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 46. Eriocheir sinensis, ZVI, mandible, CLSM images with Drishti processing. (A) mandible with developing palp, (B) lateral view of mandible with incisor teeth and molar region; both applying "large images" option with a scanned area of 1×2 fields for image stitching. Objective: 40× oil immersion. Scale bars A = 200 µm; B = 100 µm.
FIGURE 44 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 44. Eriocheir sinensis, ZVI, antennule, CLSM images with Drishti processing. (A) antennule showing small proximal seta, applying "large images" option with a scanned area of 1×2 fields for image stitching, (B) antennule rotated to show reverse angle of image A and the developing accessory flagellum bud, image merged using Adobe Photoshop. Objective: A = 20× dry; B = 40× oil immersion. Scale bars = 200 µm.
FIGURE 40 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 40. Eriocheir sinensis, ZV, second maxilliped, CLSM images with Drishti processing. (A) basis and endopod, applying "large images" option with a scanned area of 1×2 fields for image stitching (reverse angle of endopod arrowed), (B) endopod with 0,1,7 setae, (C) exopod with 12 natatory setae, (D) exopod with 13 natatory setae. Objective: 20× dry. Scale bars A, C, D = 500 µm; B = 200 µm.
FIGURE 37 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 37. Eriocheir sinensis, ZV, maxillule, CLSM images with Drishti processing. (A) maxillule, applying "large images" option with a scanned area of 2×3 fields for image stitching, (B) coxal endite, (C) basial endite. Objective: 40× oil immersion. Scale bars = 200 µm.
FIGURE 36 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 36. Eriocheir sinensis, ZV, mandible, CLSM images with Drishti processing. (A) mandible with palp bud present, (B) lateral view of mandible with incisor teeth and molar region; both applying "large images" option with a scanned area of 1×2 fields for image stitching. Objective: 40× oil immersion. Scale bars A = 200 µm; B = 100 µm.
FIGURE 35 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 35. Eriocheir sinensis, ZV, CLSM images with Drishti processing. (A) antennule with a pair of proximal setae and a developing accessory flagellum bud, (B) antennule, primary flagellum showing two rows of subterminal aesthetascs, (C) antenna with a more developed endopod and with one exopodal seta arrowed; all applying "large images" option with a scanned area of 2×3 fields for image stitching, (D) exopod of antenna with two setae. Objective: A-C = 40×; D = 60× oil immersion. Scale bars A, C = 200 µm; B = 100 µm; D = 10 µm.
FIGURE 51 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 51. Eriocheir sinensis, ZVI, CLSM images with Drishti processing. (A) maxilliped three, applying "large images" option with a scanned area of 1×2 fields for image stitching, (B) pereiopods with bilobed chela, (C) ventral view of developing fifth pleopods with endopods and uropods without endopods; both applying "large images" option with a scanned area of 2×2 fields for image stitching. Objective: 20× dry. Scale bars A = 200 µm; B-C = 500 µm.
FIGURE 32 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 32. Eriocheir sinensis, ZIV, pleon and telson, CLSM images with Drishti processing. (A) dorsal view of pleon and telson, (B) lateral view of pleon and telson; both applying "large images" option with a scanned area of 1×2 fields for image stitching, (C) pleomere 1 with 5 medial setae, (D) pleomere 1 with 2 medial setae, (E) dorsal view of telson. Objective: A-B = 10× dry; C-E =20× dry. Scale bars = 500 µm.
FIGURE 31 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 31. Eriocheir sinensis, ZIV, CLSM images with Drishti processing. (A) third maxilliped, (B) pereiopods with bilobed chela. Objective: 40× oil immersion. Scale bars = 100 µm.
FIGURE 39 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 39. Eriocheir sinensis, ZV, first maxilliped, CLSM images with Drishti processing. (A) coxa with 3 setae and basis with 10 setae, (B) coxa with 3 setae and 1 smaller seta, basis with 12 setae, (C) endopod with 1,3,2,2,6 setae, (D) endopod with 2,3,2,2,6 setae, (E) exopod with 12 natatory setae, (F) exopod with 13 natatory setae; all applying "large images" option with a scanned area of 1×2 fields for image stitching. Objective: 20× dry. Scale bars = 500 µm.
FIGURE 28 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 28. Eriocheir sinensis, ZIV, maxilla, CLSM images with Drishti processing. (A) whole appendage, applying "large images" option with a scanned area of 2×3 fields for image stitching, (B) maxilla rotated to show reverse angle of image A and the setation of the coxal and basial endites. Objective: 40× oil immersion. Scale bars = 200 µm.
FIGURE 29 in Re-description of Chinese mitten crab Eriocheir sinensis H. Milne Edwards, 1853 (Crustacea: Brachyura: Grapsoidea: Varunidae) zoeal development using confocal laser scanning microscopy
FIGURE 29. Eriocheir sinensis, ZIV, first maxilliped, CLSM images with Drishti processing. (A) whole appendage, applying "large images" option with a scanned area of 2×2 fields for image stitching, (B) endopod with 2,2,2,6 setae, (C) endopod with 2,3,2,2,6 setae, (D) exopod with 10 natatory setae. Objective: 20× dry. Scale bars A, D = 500 µm; B-C = 200 µm.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.