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Fig. 18. 3D in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 18. 3D visualizations of Micro-CT data of Zospeum simplex Inäbnit, Jochum & Neubert, 2021, (NMHW-MO-113642 ex RSC 3760) from Dobravljevac jama, Gornji Brišnik, Bosnia and Herzegovina. A–F. Specimen 1 (spm1). A. Aperture view. B. Aperture facing right view. C. Apical view. D. Dorsal view. E. Aperture facing left view. F. Ventral view. G–L. Specimen 2 (spm2). Same perspectives as Specimen 1.

opencc-by-4.0Mar 2024View details →
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Fig. 12 in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 12. Zospeum neuberti Jochum & Ruthensteiner sp. nov. (NHMW Mol.Coll.Edlauer 32006). A–B. Light microscopic images of paratypes showing aperture and dorsal views and collection labels. C. Light microscopic images of holotype showing aperture and dorsal views. D–I. 3D visualizations of Micro-CT data of holotype. D. Aperture view. E. Aperture facing right view showing smooth columella. F. Apical view. G. Dorsal view showing smooth columella. H. Aperture facing left view. I. Ventral view.

opencc-by-4.0Mar 2024View details →
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Fig. 21 in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 21. Bayesian tree of the genus Zospeum Bourguignat, 1856. Node support values of both the Bayesian Inference (left) and the Maximum Likelihood analysis (right) are given. Branches are coloured to denote the informal species groups within the eastern radiation of Zospeum following Inäbnit et al. (2019). Emboldened specimen names indicate samples sequenced for this study.

opencc-by-4.0Mar 2024View details →
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Fig. 11 in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 11. Zospeum njunjicae Jochum, Schilthuizen & Ruthensteiner sp. nov. A–B. Light microscopic images of paratypes (NMBE 572616) showing aperture and dorsal views. C. Light microscopic images of holotype (NMBE 572617) showing aperture and dorsal views. D–I. 3D visualizations of X-ray microCT data of holotype (NMBE 572617). D. Aperture view. E. Aperture facing right view showing well defined lamella. F. Apical view. G. Dorsal view. H. Aperture facing left view. I. Ventral view.

opencc-by-4.0Mar 2024View details →
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Fig. 9 in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 9. Zospeum amplioscutum Jochum & Ruthensteiner sp. nov., (NHMW Mol.Coll.Edlauer 19.069) with sample labels. A. Light microscopic images of paratype (NHMW Mol.Coll.Edlauer 19.069), apertural and dorsal views. B. Light microscopic images of holotype (NHMW Mol.Coll.Edlauer 19.069), aperture and dorsal views. C–H. 3D visualizations of X-ray Micro-CT data of holotype (NHMW Mol. Coll.Edlauer 19.069). C. Aperture view. D. Aperture facing right view. E. Apical view. F. Dorsal view. G. Aperture facing left view. H. Ventral view showing coiling projecting high up on shell and peristome oblique to umbilicus.

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Fig. 10 in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 10. Zospeum tumidum Jochum, Schilthuizen & Ruthensteiner sp. nov., holotype (NMBE 572615). A. Light microscopic images, showing aperture and dorsal views and TxEx Collection label. B. 3D visualizations of Micro-CT data, apical view. C. Ventral view showing deep umbilical depression. D. Aperture view. E. Aperture facing right view showing tapered columella and lamellar bulge. F. Dorsal view. G. Aperture facing left view.

opencc-by-4.0Mar 2024View details →
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Fig. 17. Z in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 17. Z. njegusiense Jochum & Ruthensteiner sp. nov., Scanning Electron Microscopy images. A. Holotype (NMBE 572639), aperture view showing subfossil shell with superficial erosion of shell layer. B. Paratype (NMBE 578378), aperture view showing close up of lamellar band on columella (Fig. C). C. Paratype (NMBE 578378), aperture view showing damaged shell and columella. D. Close up view of holotype (NMBE 572639), showing body whorl with low, irregular growth lines. E. Paratype (NMBE 578378), protoconch.

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Fig. 6 in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 6. Zospeum intermedium Jochum & Ruthensteiner sp. nov. (RMNH.MOL.234132) from Gittenberger (1975). A. Light microscopic images of apertural and dorsal views. B. Sample labels. C–H. 3D visualizations of X-ray Micro-CT data. C. Aperture view. D. Aperture facing right view. E. Apical view. F. Dorsal view. G. Aperture facing right view. H. Umbilical view showing slight umbilical depression and lamella projecting from the columellar side.

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Fig. 7 in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 7. Light microscopic images of shells from the caves Lipska pećina and Duboki do (Montenegro) with specimen labels. A–C. Zospeum troglobalcanicum Absolon, 1916 (MCSMNH-PMSL-Moll.-FVelkovrh 29603). D–F. Zospeum dubokidoense Jochum & Ruthensteiner sp. nov. D. Holotype (MCSMNHPMSL-Moll.-FVelkovrh 30360[spm1]). E–F. Paratypes, Duboki do, Montenegro (MCSMNH-PMSLMoll.-FVelkovrh 30360[spm2-3]).

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Fig. 2. 3D in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 2. 3D visualizations of Micro-CT data of the lectotype of Zospeum troglobalcanicum Absolon, 1916 (NHMW Moll.Coll.Edlauer 32.749) with shell parts and terms indicated. A. Measurements include shell height (sh), shell width (sw), aperture height (ah), aperture width (aw), height of last whorl (hlw) and spire angle (SA). B. Internal view showing columella (co), lamella (la), peristome (pe) and penultimate whorl (pw). C. Apical view showing protoconch (pr), teleoconch (te) and peristome (pe). D. Ventral view showing peristome configuration (pe) parietal shield (ps) and umbilicus (um).

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Fig. 4. 3D in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 4. 3D visualizations using Micro-CT data of shells from the caves Benetina pećina (SE Bosnia and Herzegovina) and Cetinska pećina (Montenegro). A–F. Zospeum troglobalcanicum Absolon, 1916, lectotype (NHMW Moll.Coll.Edlauer 32.749). A. Aperture view. B. Aperture facing right view. C. Apical view. D. Dorsal view. E. Aperture facing left view. F. Ventral view showing umbilical depression. G–L. Zospeum tortuosum Jochum & Ruthensteiner sp. nov., holotype (MCSMNH-PMSLMoll.-FVelkovrh 34099[spm1]). G. Aperture view. H. Aperture facing right view. I. Apical view. J. Dorsal view. K. Aperture facing left view. L. Ventral view showing callused peristome positioned mostly on top of umbilicus.

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Fig. 15 in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 15. Zospeum biokovoense Jochum & Ruthensteiner sp. nov. (NHMW Mol.Coll.Edlauer 16.390). A–B. Light microscopic images showing aperture and dorsal views and collection labels. A. Juvenile shell. B. Holotype. C–H. 3D visualizations of Micro-CT data of holotype. C. Aperture view showing gnarled deformation of the columella. D. Aperture facing right view showing fat columella and lamella. E. Apical view. F. Dorsal view showing lopsided spire and incomplete lamella on top of fat columella. G. Aperture facing left view. H. Ventral view showing puckered umbilical depression.

opencc-by-4.0Mar 2024View details →
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Fig. 5. 3D in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 5. 3D visualizations of Micro-CT data of the deformed shell, (MCSMNH-PMSL-Moll.-FVelkovrh 34099[spm4]) from Cetinska pećina. A. Aperture view. B. Aperture facing right view. C. Apical view. D. Dorsal view. E. Aperture facing left view. F. Ventral view with umbilicus covered by last quarter of shell whorl.

opencc-by-4.0Mar 2024View details →
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Fig. 1 in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 1. Map showing Western Balkan cave localities of populations of Zospeum Bourguignat, 1856. Cave localities are indicated in red.

opencc-by-4.0Mar 2024View details →
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Fig. 3 in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 3. Light microscopic images of shells from the caves Benetina pećina (SE Bosnia and Herzegovina) and Cetinska pećina (Montenegro) with specimen labels. A. Zospeum troglobalcanicum Absolon, 1916, lectotype (NHMW Moll.Coll.Edlauer 32.749) from Benetina pećina. B–C. Zospeum tortuosum Jochum & Ruthensteiner sp. nov., paratypes (MCSMNH-PMSL-Moll.-FVelkovrh 34099[spm2-3]) from Cetinska pećina. D. Zospeum tortuosum Jochum & Ruthensteiner sp. nov., holotype (MCSMNHPMSL-Moll.-FVelkovrh 34099[spm1]) from Cetinska pećina. E. Deformed shell (MCSMNH-PMSLMoll.-FVelkovrh 34099[spm4]) from Cetinska pećina.

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Fig. 14. 3D in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 14. 3D visualizations of Micro-CT data of Zospeum constrictum Jochum & Ruthensteiner sp. nov. (NHMW Mol.Coll.Edlauer 16.693). A–F. Holotype. A. Aperture and dorsal views. B. Aperture facing right view. C. Apical view showing upper right side of final whorl tucked underneath spire. D. Dorsal view. E. Aperture facing left view. F. Ventral view. G–L. Paratype (Fig. 13B). G. Aperture view. H. Aperture facing right view. I. Apical view showing upper right side of final whorl tucked underneath spire. J. Dorsal view. K. Aperture facing left view. L. Ventral view.

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Fig. 20. 3D in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 20. 3D visualizations of Micro-CT data of Zospeum sp. 2, Taleža pećina, bei Trebinje, BiH, presented as Z. troglobalcanicum in Inäbnit et al. 2021 (NMBE 553414/1 (ex RSC 1981). A. Aperture view showing low, ridge-like fold on inner penultimate whorl. B. Aperture facing right view showing smooth columella. C. Apical view. D. Dorsal view. E. Aperture facing right view. F. Ventral view showing peristome edge directly parallel to umbilical depression.

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Fig. 8. 3D in 3D X-ray microscopy (Micro-CT) and SEM reveal Zospeum troglobalcanicum Absolon, 1916 and allied species from the Western Balkans (Ellobioidea: Carychiidae)

Fig. 8. 3D visualizations of Micro-CT data of shells from the caves Lipska pećina and Duboki do (Montenegro). A–F. Zospeum troglobalcanicum Absolon, 1916 (MCSMNH-PMSL-Moll.-FVelkovrh 29603) (Fig. 7A). A. Aperture view. B. Aperture facing right view. C. Apical view. D. Dorsal view. E. Aperture facing left view. F. Ventral view showing reduced umbilical depression. G–L. Zospeum dubokidoense Jochum & Ruthensteiner sp. nov., holotype (MCSMNH-PMSL-Moll.-FVelkovrh 30360[spm1]). G. Aperture view. H. Aperture facing right view. I. Apical view. J. Dorsal view. K. Aperture facing left view. L. Ventral view showing no umbilical depression.

opencc-by-4.0Mar 2024View details →
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Supplementary material S2. Leiestes tomaszewskae sp. nov., holotype, Nr. 6723 [MAIG], X-ray micro-CT volume rendering of the habitus (without legs).

<p>X-ray micro-CT volume rendering of the habitus (without legs) of <em>Leiestes</em> <em>tomaszewskae</em> sp. nov., holotype, Nr. 6723 [MAIG].</p>

opencc-by-4.0Aug 2022View details →
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Supplementary material S1. Leiestes tomaszewskae sp. nov., holotype, Nr. 6723 [MAIG], X-ray micro-CT volume rendering of the habitus.

<p>X-ray micro-CT volume rendering of the habitus of <em>Leiestes</em> <em>tomaszewskae</em> sp. nov., holotype, Nr. 6723 [MAIG].</p>

opencc-by-4.0Aug 2022View details →

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