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83 results for “microsatellite loci”
Data from: Rapid development and screening of microsatellite loci for Artibeus lituratus and their utility for six related species within Phyllostomidae
Microsatellites are often the marker of choice for population genetic studies at intermediate spatial and temporal scales. Developing large numbers of markers has traditionally been technically difficult and this has limited our ability to investigate evolutionary phenomena that emerge across short temporal scales. Moreover, few markers tend to successfully amplify across species boundaries. As rapid advancements in high-throughput sequencing make microsatellite development cost and time-effective, new avenues for evolutionary, population genetic, and chromosome linkage mapping research are emerging. We used a published PERL script and second-generation sequencing to rapidly and affordably develop microsatellite loci for a widespread phyllostomid bat, Artibeus lituratus, for which no markers were previously available. We used Roche FLX (Titanium) Genome Sequencing to randomly sequence ~101 Mb (255,065 unique reads) of genomic DNA for A. lituratus, within which we discovered 30100 microsatellite loci. We designed primers for 19395 loci that contained suitable flanking regions. We ordered primers for 96 loci, 90 of which produced a single PCR product in A. lituratus. We genotyped 52 loci, and 45 were polymorphic in A. lituratus. We tested cross-species amplification for 96 loci in six additional phyllostomid species: A. planirostris, A. fimbriatus, A. phaeotis, Enchisthenes hartii, Sturnira lilium, and Carollia perspicillata. Cross-species amplification was successful for at least one species for 87 loci (A. fimbriatus), and in all species at least 66 loci amplified. These markers will facilitate future work on these seven species, but also illustrate the utility of this high-throughput method for development of primers across many species simultaneously.
Data from: Isolation and characterization of fifteen polymorphic microsatellite loci for the citrus mealybug, Planococcus citri (Hemiptera: Pseudococcidae), and cross-amplification in two other mealybug species
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Data from: Characterization of microsatellite loci for the Gulf Coast waterdog (Necturus beyeri) using paired-end Illumina shotgun sequencing and cross-amplification in other Necturus
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FIGURE 2 in Development of microsatellite loci and population genetics in the bumblebee catfish species Pseudopimelodus atricaudus and Pseudopimelodus magnus (Siluriformes: Pseudopimelodidae)
FIGURE 2 | Population structure suggested by STRUCTURE (A-C) and the Discriminant Analysis of the Principal Components (D, E) for Pseudopimelodus magnus (A, B, D), and Pseudopimelodus atricaudus (A, C, E). S1: sector 1 of the middle Cauca River; S4, S5, and S8: sectors 4, 5 and 8 of the lower Cauca River, respectively.
FIGURE 1 in Microsatellite loci development for three catfish species from northwestern South America
FIGURE 1 | Frequency of identified microsatellite loci and potentially amplifiable loci (PAL) obtained from 454 FLX sequencing for Ageneiosus pardalis, Pimelodus grosskopfii, and Sorubim cuspicaudus.
Figure 3 from: Li Y, Lin L-S, Gao T-X (2018) Genetic signatures of polymorphic microsatellite loci in the Ambiguous silver pomfret, Pampus argenteus (Teleostei, Stromateidae). ZooKeys 810: 139-151. https://doi.org/10.3897/zookeys.805.25602
Figure 3 3D-FCA showing relationships among six populations of P.argenteus based on seven microsatellite loci. Abbreviations: SO: Sonmiani Bay, OR: Ormara, PS: Pasni, KW: Kuwait, TW: Taiwan, XM: Xiamen.
Figure 4 from: Li Y, Lin L-S, Gao T-X (2018) Genetic signatures of polymorphic microsatellite loci in the Ambiguous silver pomfret, Pampus argenteus (Teleostei, Stromateidae). ZooKeys 810: 139-151. https://doi.org/10.3897/zookeys.805.25602
Figure 4 Results of the STRUCTURE analysis from seven microsatellite loci in P.argenteus (K = 2). Vertical lines are proportional to the probability of individual membership in the simulated cluster. Abbreviations: SO: Sonmiani Bay, OR: Ormara, PS: Pasni, KW: Kuwait, TW: Taiwan, XM: Xiamen.
Figure 2 from: Li Y, Lin L-S, Gao T-X (2018) Genetic signatures of polymorphic microsatellite loci in the Ambiguous silver pomfret, Pampus argenteus (Teleostei, Stromateidae). ZooKeys 810: 139-151. https://doi.org/10.3897/zookeys.805.25602
Figure 2 The UPGMA tree based on (δμ)2 genetic distance of six P.argenteus populations. Abbreviations: SO: Sonmiani Bay, OR: Ormara, PS: Pasni, KW: Kuwait, TW: Taiwan, XM: Xiamen.
Microsatellite genotype data from seven loci for a phylogeographic/population genetic study of the South African endemic freshwater crab Potamonautes lividus sampled from eight localities in the KwaZulu-Natal and Eastern Cape provinces in South Africa
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Data from: Isolation and characterization of 145 polymorphic microsatellite loci for the common frog (Rana temporaria)
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Data from: Isolation and characterization of fifteen polymorphic microsatellite loci for the citrus mealybug, Planococcus citri (Hemiptera: Pseudococcidae), and cross-amplification in two other mealybug species
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Data from: Microsatellite loci for dreissenid mussels (Mollusca: Bivalvia: Dreissenidae) and relatives: markers for assessing exotic and native populations
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Data from: Characterization of microsatellite loci and reliable genotyping in a polyploid plant, Mercurialis perennis (Euphorbiaceae)
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Data from: Rapid development and screening of microsatellite loci for Artibeus lituratus and their utility for six related species within Phyllostomidae
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Data from: Characterization of 42 polymorphic microsatellite loci in Mimulus ringens (Phrymaceae) using Illumina sequencing
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Data from: Characterization of microsatellite loci for the Gulf Coast waterdog (Necturus beyeri) using paired-end Illumina shotgun sequencing and cross-amplification in other Necturus
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Data from: Isolation of microsatellite loci by next-generation sequencing of the critically endangered Northern Bald ibis, Geronticus eremita
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Data from: Isolation and characterization of microsatellite loci for the isopod crustacean Armadillidium vulgare and transferability in terrestrial isopods
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Data from: ‘True’ null allele detection in microsatellite loci: a comparison of methods, assessment of difficulties, and survey of possible improvements
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Figure 1 from: Li Y, Lin L-S, Gao T-X (2018) Genetic signatures of polymorphic microsatellite loci in the Ambiguous silver pomfret, Pampus argenteus (Teleostei, Stromateidae). ZooKeys 810: 139-151. https://doi.org/10.3897/zookeys.805.25602
Figure 1 Locations (black circle) for sample collection of P.argenteus.
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.