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FIGURE 4 in A molecular phylogenetic study on South Korean Tettigonia species (Orthoptera: Tettigoniidae) using five genetic loci: The possibility of multiple allopatric speciation

FIGURE 4. Inter- (gray) and intraspecific (open) genetic differences in Tettigonia species for CO1 calculated using the pdistance method and treatment of pairwise deletion for gaps with the range of genetic difference within clusters. The box plot displays the median (internal transverse thick line) and interquartile range (box). Short lines indicate maximum and minimum genetic differences. Asterisk denotes a sequence from NCBI; T. viridissima, JN609414–JN609420; T. hispania, EF515121; T. chinensis, HQ609468–HQ609470. (JJ-TU = Jeju Island population of T. ussuriana; JS-TU = Jeongseon population of T. ussuriana; PC-TU = Pyeongchang population of T. ussuriana; MJ-TU = Muju population of T. ussuriana; MG-TU = Mungyeong population of T. ussuriana)

FIGURE 3 in A molecular phylogenetic study on South Korean Tettigonia species (Orthoptera: Tettigoniidae) using five genetic loci: The possibility of multiple allopatric speciation

FIGURE 3. Neighbor-joining tree inferred from the concatenated dataset of all five genetic loci: CO1, CO2, ND1, TA1, and ITS2. Neighbor-joining (left) and parsimony (right) bootstrap values are indicated above internodes; Bayesian posterior probabilities are shown below internodes.

FIGURE 2 in A molecular phylogenetic study on South Korean Tettigonia species (Orthoptera: Tettigoniidae) using five genetic loci: The possibility of multiple allopatric speciation

FIGURE 2. Neighbor-joining (A), parsimony (B), and Bayesian inference (C) trees inferred from the combined dataset of three mtDNA loci (CO1 + CO2 + ND1). Numbers next to nodes are bootstrap or posterior probability values.

FIGURE 1 in A molecular phylogenetic study on South Korean Tettigonia species (Orthoptera: Tettigoniidae) using five genetic loci: The possibility of multiple allopatric speciation

FIGURE 1. Neighbor-joining phylogenetic tree of each mtDNA gene analysis: (A) CO1, (B) CO2, (C) ND1. Numbers next to nodes are bootstrap values. Numbers on arrows are genetic differences between two clusters.

FIGURE 19 in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURE 19. Distribution of the genus Hungarosoma Verhoeff, 1928. Empty dot: H. inexpectatum, solid dots: H. bokori. Distribution of H. bokori in Slovak-Aggtelek Karst drawn in higher scale.

FIGURE 16. Hungarosoma bokori Verhoeff, 1928 in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURE 16. Hungarosoma bokori Verhoeff, 1928, male, gonopods (Abaliget Cave). Right lateral view. Letters a–h signal equivalent structures in both views. Abbreviations: Letters a–h signal equivalent structures in both views. Anterior gonopods (legs 8): a = cheirite, b = brush-like arm, c = additive divided arm, d = hyaline process. Posterior gonopods (legs 9): e = gonopod, f = ventral hyaline prominence, g = claw shape process with long seta.

FIGURE 18. A in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURE 18. A Maximum-Likelihood tree (GTR+G+I model) based on the COI gene and rooted with Polyxenus lagurus. All data—except from H. bokori—were obtained from Genbank. Numbers refer to bootstrap values (1000 replicates). Scale bar = 0.02 substitutions/site. For origin of the H. bokori material, see Table 1.

FIGURE 15. Hungarosoma bokori Verhoeff, 1928 in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURE 15. Hungarosoma bokori Verhoeff, 1928, male, gonopods (Abaliget Cave). Anterior view (right side of pair structures is slightly turned laterally). Abbreviations: Letters a–h signal equivalent structures in both views. Anterior gonopods (legs 8): a = cheirite, b = brush-like arm, c = additive divided arm, d = hyaline process. Posterior gonopods (legs 9): e = gonopod, f = ventral hyaline prominence, g = claw shape process with long seta.

FIGURES 12–14. Hungarosoma bokori Verhoeff, 1928 in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURES 12–14. Hungarosoma bokori Verhoeff, 1928, male (Abaliget Cave). 12: Antenna. 13: Gonopod complex, anterior view. The right side of pair structures is slightly turned laterally. 14: Gonopods in right lateral view. Abbreviations: Letters a– h signal equivalent structures in both views. Anterior gonopods (legs 8): a = cheirite, b = brush-like arm, c = additive divided arm, d = hyaline process. Posterior gonopods (legs 9): e = gonopod, f = ventral hyaline prominence, g = claw shape process with long seta. Not scaled. Photos: Andrej Mock.

FIGURES 10–11. Hungarosoma bokori Verhoeff, 1928 in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURES 10–11. Hungarosoma bokori Verhoeff, 1928, female from the Driny Cave, scanning electronic microscopy of details of the shape and surface of mid-body segments. 10: Dorsolateral view (left side). 11: A pleurotergite, dorsolateral view in detail. Photos: Andrej Mock & Karel Tajovský.

FIGURES 2–5. Hungarosoma bokori Verhoeff, 1928 in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURES 2–5. Hungarosoma bokori Verhoeff, 1928, female, holotype (Abaliget Cave). 2: Head end of the body, right lateral view. 3: Tergite 15, dorsal view. 4: Antenna, lateral view. 5: Discernable vulvae in situ (v), right lateral view. Photos: Jörg Spelda.

FIGURE 1. Hungarosoma bokori Verhoeff, 1928 in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURE 1. Hungarosoma bokori Verhoeff, 1928, female, sampled at the entrance of the Baradla Cave, Hungary, 21.iii.2013. Photo: Ľubomír Kováč & Andrej Mock.

FIGURES 20–21. 20 in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURES 20–21. 20. Distribution of localities with Hungarosoma spp. along the altitudinal gradient. H. inexpectatum (empty dot) was found in the town of Cluj (Romania, 350 m a.s.l.), other values belong to the localities with records of H. bokori (solid dots). 21. Seasonality of records of Hungarosoma spp. (based on original and available published data).

FIGURES 6–9. Hungarosoma bokori Verhoeff, 1928 in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURES 6–9. Hungarosoma bokori Verhoeff, 1928, specimens from the Abaliget Cave, preserved in alcohol (not scaled). 6: Habitus of adult male in lateral view; the cheirites of anterior gonopods are visible. 7: Details of the dorsal part of the male trunk. 8: Ventral side of mid-body segments in detail. 9: Dorsal side of a juvenile of stadium III with the shape of the pleurotergites typical for the genus (all material from the Abaliget Cave, Hungary). Photos: Andrej Mock.

FIGURE 17. Hungarosoma bokori Verhoeff, 1928 in Hungarosoma bokori Verhoeff, 1928 (Diplopoda: Chordeumatida): new insights into its taxonomy, systematics, molecular genetics, biogeography and ecology

FIGURE 17. Hungarosoma bokori Verhoeff, 1928, female, vulvae (Driny Cave). Vulvae in posterior-ventral view (o = opercula) Not scaled.

opencc-by-4.0Nov 2024View details →

Systematic Identification of Needlefish (Belonidae) Species using Molecular Genetic and Morphological Markers in the Mediterranean and Black Seas

In this study, we aimed to clarify the taxonomic status of Belonidae species distributed in the Mediterranean Sea and the Black Sea by conducting detailed genetic and morphological markers. A total of 550 needlefish samples were caught between January 2022 and January 2024.  The data set used in the study contains a total of 171 sequences for the COI gene and 120 sequences for the 12s rRNA gene from different Belonidae species, including data from GenBank. Systematic analysis of needlefish species was investigated by using sequencing of mtDNA COI and 12s rRNA gene regions and morphological characters in the Turkish Marine Waters. A separate analysis of the two mitochondrial genes supported by morphological characters revealed that each species is grouped within itself. The genetic and morphological analyses showed that Belone belone acus and Belone belone euxini which are considered as the subspecies of Belone belone are not subspecies of the genus Belone and should be considered at the species level, Belone belone.  Belone svetovidovi is also considerably different from Belone belone and should be considered as a different species. T. acus imperialis, which is thought to be distributed in the Mediterranean Sea, is not a subspecies of Tylosorus acus and should be revised as Tylosorus imperialis which genetically differs from Tylosorus acus and also other Tylosorus species at the species level.

FIGURE 6 in Assessment of SCoT and ISSR molecular markers in genetic diversity of rigid ryegrass (Lolium rigidum Gaud.) in Iran

FIGURE 6. STRUCTURE analysis of L. rigidum populations (A: the relationship between k and Delta k; the grouping based on k=18 (top) and k=17 (below) (The population code is according to Table 1).

FIGURE 3 in Assessment of SCoT and ISSR molecular markers in genetic diversity of rigid ryegrass (Lolium rigidum Gaud.) in Iran

FIGURE 3. TCS Network of L. rigidum studied populations based on ISSR data (The population code is according to Table 1) (Numbers of branches reveal number of different loci among studied populations).

FIGURE 5 in Assessment of SCoT and ISSR molecular markers in genetic diversity of rigid ryegrass (Lolium rigidum Gaud.) in Iran

FIGURE 5. NeighborNet diagram of L. rigidum studied populations based on SCoT data (The population code is according to Table 1).

FIGURE 2 in Assessment of SCoT and ISSR molecular markers in genetic diversity of rigid ryegrass (Lolium rigidum Gaud.) in Iran

FIGURE 2. UPGMA dendrogram of L. rigidum specimens showing genetic differences of the studied populations based on ISSR data (The population code is according to Table 1).