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1,076 results for “Metabarcoding”
Supplementary material 4 from: Weigand AM, Macher J-N (2018) A DNA metabarcoding protocol for hyporheic freshwater meiofauna: Evaluating highly degenerate COI primers and replication strategy. Metabarcoding and Metagenomics 2: e26869. https://doi.org/10.3897/mbmg.2.26869
Table S1: Sampling site coordinates :
Supplementary material 1 from: Weigand AM, Macher J-N (2018) A DNA metabarcoding protocol for hyporheic freshwater meiofauna: Evaluating highly degenerate COI primers and replication strategy. Metabarcoding and Metagenomics 2: e26869. https://doi.org/10.3897/mbmg.2.26869
DNA extraction protocol :
Supplementary material 1 from: Majaneva M, Diserud OH, Eagle SHC, Hajibabaei M, Ekrem T (2018) Choice of DNA extraction method affects DNA metabarcoding of unsorted invertebrate bulk samples. Metabarcoding and Metagenomics 2: e26664. https://doi.org/10.3897/mbmg.2.26664
Fragment and working PCR conditions. :
Figure 3 from: Anslan S, Nilsson RH, Wurzbacher C, Baldrian P, Tedersoo L, Bahram M (2018) Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding. MycoKeys 39: 29-40. https://doi.org/10.3897/mycokeys.39.28109
Figure 3 - Number of OTUs per sample for Illumina data recorded from a) pipeline-generated OTU tables (median differences = 38 OTUs) and from b) filtered OTU tables (median differences = 12 OTUs). The Galaxy workflow was excluded here.
Figure 2 from: Anslan S, Nilsson RH, Wurzbacher C, Baldrian P, Tedersoo L, Bahram M (2018) Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding. MycoKeys 39: 29-40. https://doi.org/10.3897/mycokeys.39.28109
Figure 2 - OTU accumulation curves of the evaluated pipelines for a) PacBio and b) Illumina datasets.
Supplementary material 5 from: Cahoon AB, Huffman AG, Krager MM, Crowell RM (2018) A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA. Metabarcoding and Metagenomics 2: e26939. https://doi.org/10.3897/mbmg.2.26939
Table 3. Diversity Indices. :
Supplementary material 3 from: Cahoon AB, Huffman AG, Krager MM, Crowell RM (2018) A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA. Metabarcoding and Metagenomics 2: e26939. https://doi.org/10.3897/mbmg.2.26939
Table 1. All Detected Taxa :
Supplementary material 1 from: Cahoon AB, Huffman AG, Krager MM, Crowell RM (2018) A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA. Metabarcoding and Metagenomics 2: e26939. https://doi.org/10.3897/mbmg.2.26939
Figure 1. Sample collection sites :
Supplementary material 6 from: Cahoon AB, Huffman AG, Krager MM, Crowell RM (2018) A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA. Metabarcoding and Metagenomics 2: e26939. https://doi.org/10.3897/mbmg.2.26939
Table 4. The protist genera identified in Natural Tunnel State Park organised by read count. :
Supplementary material 4 from: Cahoon AB, Huffman AG, Krager MM, Crowell RM (2018) A meta-barcoding census of freshwater planktonic protists in Appalachia – Natural Tunnel State Park, Virginia, USA. Metabarcoding and Metagenomics 2: e26939. https://doi.org/10.3897/mbmg.2.26939
Table 2. Primers and PCR Conditions. :
Supplementary material 1 from: Beentjes KK, Speksnijder AGCL, Schilthuizen M, Schaub BEM, van der Hoorn BB (2018) The influence of macroinvertebrate abundance on the assessment of freshwater quality in The Netherlands. Metabarcoding and Metagenomics 2: e26744. https://doi.org/10.3897/mbmg.2.26744
Monitoring event details and EQR scores :
Supplementary material 5 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
Alternative statistical model :
Supplementary material 4 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
4_NTC :
Supplementary material 2 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
Bioinformatic pipeline and thresholds :
Supplementary material 3 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
Profiling tables for all libraries :
Supplementary material 1 from: Deiner K, Lopez J, Bourne S, Holman LE, Seymour M, Grey EK, Lacoursière-Roussel A, Li Y, Renshaw MA, Pfrender ME, Rius M, Bernatchez L, Lodge DM (2018) Optimising the detection of marine taxonomic richness using environmental DNA metabarcoding: the effects of filter material, pore size and extraction method. Metabarcoding and Metagenomics 2: e28963. https://doi.org/10.3897/mbmg.2.28963
Extraction protocols :
Figure 1 from: Riit T, Tedersoo L, Drenkhan R, Runno-Paurson E, Kokko H, Anslan S (2018) Corrigendum for: "Oomycete-specific ITS primers for identification and metabarcoding" published in MycoKeys, doi: 10.3897/mycokeys.14.9244. MycoKeys 41: 119-120. https://doi.org/10.3897/mycokeys.41.30558
Figure 1 A Map of universal and oomycete-specific ITS region primers B Taxa with mismatches in the binding sites of primers ITS1oo and ITS3oo. Only taxa with 10% or more mismatching accessions are shown.
Supplementary material 1 from: Bylemans J, Gleeson DM, Lintermans M, Hardy CM, Beitzel M, Gilligan DM, Furlan EM (2018) Monitoring riverine fish communities through eDNA metabarcoding: determining optimal sampling strategies along an altitudinal and biodiversity gradient. Metabarcoding and Metagenomics 2: e30457. https://doi.org/10.3897/mbmg.2.30457
: Data type: Microsoft Word Document (.docx)
Supplementary material 2 from: Bylemans J, Gleeson DM, Lintermans M, Hardy CM, Beitzel M, Gilligan DM, Furlan EM (2018) Monitoring riverine fish communities through eDNA metabarcoding: determining optimal sampling strategies along an altitudinal and biodiversity gradient. Metabarcoding and Metagenomics 2: e30457. https://doi.org/10.3897/mbmg.2.30457
: Data type: statistical data
Supplementary material 1 from: Gueidan C, Elix JA, McCarthy PM, Roux C, Mallen-Cooper M, Kantvilas G (2019) PacBio amplicon sequencing for metabarcoding of mixed DNA samples from lichen herbarium specimens. MycoKeys 53: 73-91. https://doi.org/10.3897/mycokeys.53.34761
: Data type: measurement
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