Find research datasets worth reusing
Search datasets from major research repositories and use ShareScore to quickly assess how well each record supports discovery, access, and reuse.
882
datasets available to search
ShareScore release 0.9.0
Dataset results
882 results for “3d model”
Modeling of TDP-43 proteinopathy by chronic oxidative stress identifies rapamycin as beneficial in ALS patient-derived 2D and 3D iPSC models
<p>Amyotrophic Lateral Sclerosis (ALS) is a fatal neurodegenerative disorder characterized by TDP-43 proteinopathy for which the hypothesized pathomechanisms are the loss of TDP-43 nuclear function and the concomitant toxic gain of function of cytoplasmic and phosphorylated TDP-43 (P-TDP-43) aggregates. Response to environmental insults and formation of stress granules (SGs) have been proposed as initiators of TDP-43 pathological aggregation. Aim of this study was to generate <em>in vitro</em> models of TDP-43 proteinopathy to be used for drug screening. We first induced a chronic oxidative insult in human SK-N-BE cells by exposure to low doses of sodium arsenite (ARS). Our data showed TDP-43 mislocalization into the cytoplasm, insoluble P-TDP-43 increase, P62 accumulation, and defective splicing activity of TDP-43 towards its target genes <em>UNC13A</em> and <em>POLDIP3</em> as readouts of TDP-43 nuclear loss-of-function. As autophagy impairment plays a crucial role in ALS, we tested three drugs involved in promoting autophagy, namely rapamycin, lithium carbonate, and metformin: only rapamycin was able to rescue ARS-induced loss of TDP-43 splicing activity on <em>UNC13A</em>. We then tested rapamycin in ALS patient-derived primary fibroblasts, where it reduced P-TDP-43 aggregates and SGs formation. Rapamycin was also tested in motor neurons differentiated from induced pluripotent stem cells (iPSCs) derived from ALS patients, where we confirmed its efficacy in reducing P-TDP-43 aggregates and SGs and rescuing ARS-induced loss of TDP-43 splicing activity. Finally, we tested rapamycin in ALS iPSC-derived brain organoids exposed to chronic ARS, where it rescued loss of TDP-43 splicing activity. In conclusion, we established different human cell models of TDP-43 proteinopathy in which rapamycin was able to rescue chronic oxidative stress-induced alterations in TDP-43 splicing activity and cytoplasmic mislocalization by modulating autophagy. Human SK-N-BE and ALS patient-derived 2D and 3D iPSC models chronically treated with ARS can therefore be exploited as <em>in vitro</em> platforms for future drug screening approaches.</p>
NASA 3D Models: Canadarm
Polygons: 663 Vertices: 1080
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.