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FIGURE 11 in A new genus of anthophilous drosophilids, Impatiophila (Diptera, Drosophilidae): morphology, DNA barcoding and molecular phylogeny, with descriptions of thirty-nine new species

FIGURE 11. The strict consensus tree of 1382 most parsimonious cladograms. This tree was obtained under the constraints of tree topology shown in Fig. 10 by a grafting analysis with 25 morphological characters. Synapomorphies inferred from ACCTRAN character optimization are indicated on each branch: solid circle, non-homoplastic change; open circle, homoplastic (parallel and/or reversal) change; bar, change inconsistent with DELTRAN inference (not shown). Bootstrap value (more than 50%) is indicated beside each branch. * Morphologically grafted species.

FIGURE 10. A in A new genus of anthophilous drosophilids, Impatiophila (Diptera, Drosophilidae): morphology, DNA barcoding and molecular phylogeny, with descriptions of thirty-nine new species

FIGURE 10. A backbone constraint topology. Branches with ≥ 50% ML bootstrap percentages in the molecular phylogeny (Fig. 9) were retained. This constraint was used for searching of parsimony trees in a grafting analysis with morphological characters. Numbers beside each node are posterior probability and bootstrap percentage (separated by a slash).

FIGURE 15 in A new genus of anthophilous drosophilids, Impatiophila (Diptera, Drosophilidae): morphology, DNA barcoding and molecular phylogeny, with descriptions of thirty-nine new species

FIGURE 15. Impatiophila eretmosternata Fu & Gao, sp. nov. Adult male (holotype, #00515) and female (paratype, #00310): A, periphallic organs (caudolateral view); B, caudoventral part of epandrium; C, surstylus (caudal view); D, tenth sternite; E, phallic organs (dorsal view); F, phallic organs (lateral view); G, oviscapt (lateral view); H, oviscapt (ventral view).

FIGURE 14 in A new genus of anthophilous drosophilids, Impatiophila (Diptera, Drosophilidae): morphology, DNA barcoding and molecular phylogeny, with descriptions of thirty-nine new species

FIGURE 14. Impatiophila convergens Fu & Gao, sp. nov. Adult male (holotype, #00311) and female (paratype, #00540): A, periphallic organs (caudolateral view); B, caudoventral part of epandrium; C, surstylus (caudal view); D, tenth sternite; E, phallic organs (dorsal view); F, phallic organs (lateral view); G, oviscapt (lateral view); H, oviscapt (ventral view).

FIGURE 2. Collection sites. 1 in A new genus of anthophilous drosophilids, Impatiophila (Diptera, Drosophilidae): morphology, DNA barcoding and molecular phylogeny, with descriptions of thirty-nine new species

FIGURE 2. Collection sites. 1, along the way from Lage Village, Baiba Town, Linzhi County to Hanmi Village, Beibeng Town, Motuo County, Xizang; 2, Hanmi Village; 3, along the way from Hanmi to the seat of Beibeng Town; 4, the seat of Beibeng Town; 5, from the seat of Beibeng Town to Yarang Village, Motuo Town, Motuo County, Xizang; 6, from Yarang to the seat of Motuo County; 7, Tongmai Village, Yigong Town, Bomi County, Xizang; 8, Hutiaoxia Town, Shangri-la County, Yunnan; 9, Liangjiaren (site 2) in Hutiaoxia Town; 10, Cangshan National Nature Reserve, Dali, Yunnan; 11, Yangbi County, Yunnan; 12, Jiujiezi, Mt. Gaoligong, Baoshan, Yunnan; 13, Baihualing Village, Mangkuan Town, Longyang District; Baoshan, Yunnan; 14, Hesong Village, Xiding Town, Menghai County, Yunnan; 15, Yixiang Town, Simao District, Pu'er, Yunnan; 16, Mt. Huanglianshan, Luchun County, Yunnan; 17, Wenjing Town or Jinping Town, Jingdong County, Yunnan; 18, Qiongzhusi, Kunming, Yunnan; 19, Mt. E'mei, Emeishan City, Sichuan; 20, Haoping, Taibaishan National Nature Reserve, Shaanxi; 21, Shennongjia Forestry District, Shiyan, Hubei; 22, Maoershan National Nature Reserve, Guilin, Guangxi; 23, Chitou, Nantou, Taiwan, China.

FIGURE 9 in A new genus of anthophilous drosophilids, Impatiophila (Diptera, Drosophilidae): morphology, DNA barcoding and molecular phylogeny, with descriptions of thirty-nine new species

FIGURE 9. Bayesian tree of Impatiophila. Bayesian posterior probabilities and ML bootstrap percentages (BP) are shown (separated by a slash) for branches supported by BPs ≥ 50%.

FIGURE 7 in A new genus of anthophilous drosophilids, Impatiophila (Diptera, Drosophilidae): morphology, DNA barcoding and molecular phylogeny, with descriptions of thirty-nine new species

FIGURE 7. Neighbor-joining tree of the COI DNA sequences of BVDs. Label of each operational taxonomic unit (OUT) is given in the format of "voucher number (collection site code, sex)" (see Table 1 and Fig. 2 for the details of the code of each collection site). Two new species, I. viasericaria and I. chiasmosternata spp. nov., are framed with dashed line in the parts A and B of the tree, respectively. Numbers beside nodes are bootstrap percentages.

FIGURE 13 in A new genus of anthophilous drosophilids, Impatiophila (Diptera, Drosophilidae): morphology, DNA barcoding and molecular phylogeny, with descriptions of thirty-nine new species

FIGURE 13. Impatiophila parvula Fu & Gao, sp. nov. Adult male (paratype, #00270) and female (paratype, #00612): A, periphallic organs (caudolateral view); B, caudoventral part of epandrium; C, surstylus (caudal view); D, tenth sternite; E, phallic organs (dorsal view); F, phallic organs (lateral view); G, oviscapt (lateral view); H, oviscapt (ventral view). Abbreviations: aed = aedeagus, aed a = aedeagal apodeme, aed b p = aedeagal basal process, cerc = cercus, epand = epandrium, epand a = epandrial apodeme, gon = gonopod, hypd = hypandrium, pm = paramere, sur = surstylus. Scale lines = 0.1 (solid lines) or 0.05 mm (dashed lines).

FIGURE 6. Characters 15–25 in A new genus of anthophilous drosophilids, Impatiophila (Diptera, Drosophilidae): morphology, DNA barcoding and molecular phylogeny, with descriptions of thirty-nine new species

FIGURE 6. Characters 15–25: female terminalia. A–L, oviscapt valve of Impatiophila unicolorata (A, lateral view; B, ventral view; #01439), I. viasericaria (C, lateral view; D, ventral view; #01406), I. latipennata (E, lateral view; F, ventral view; #00303), I. magnimaculata (G, lateral view; #01572), I. curvivalva (H, enlarged apical portion in lateral view; I, lateral view; #00097), I. tumidivalva (J, lateral view; #00376) and I. bifurcata (K, lateral view; L, ventral view; #01127); M, N, spermatheca (lateral view) of I. yapingi (M, ♀ from Kunming, Yunnan, China) and I. actinia (N, ♀ from Chitou, Taiwan).

FIGURE 5. Characters 8–14 in A new genus of anthophilous drosophilids, Impatiophila (Diptera, Drosophilidae): morphology, DNA barcoding and molecular phylogeny, with descriptions of thirty-nine new species

FIGURE 5. Characters 8–14: male terminalia. A–F, periphallic organs (caudolateral view) of Impatiophila securiformis (A, #01501), I. longifolia (B, #00508), I. motuoensis (C, #00273), I. epubescens (D, #00280), I. pentamaculata (E, ♂ from Jizushan, Dali, Yunnan, China) and Lissocephala sabroskyi (F, ♂ from Bogor, West Java, Indonesia); G–J, phallic organs (lateral view) of I. curvivalva (G, H; #00089) and I. menba (I, J; #00307).

FIGURES 32–35 in Cryptic diversity and phylogeography of high alpine Sattleria — a case study combining DNA barcodes and morphology (Lepidoptera: Gelechiidae)

FIGURES 32–35. Male genitalia of Sattleria (details of valva-vinculum complex). 32, S. graiaeella sp. nov., paratype, slide GEL 1135; 33, ditto, paratype, slide GEL 1136; 34, S. basistrigella Huemer, slide GEL 1139; 35, S. triglavica Povolný, paratype, slide Mus. Vind. 11202.

FIGURES 17–20 in Cryptic diversity and phylogeography of high alpine Sattleria — a case study combining DNA barcodes and morphology (Lepidoptera: Gelechiidae)

FIGURES 17–20. Male genitalia of Sattleria. 17, S. graiaeella sp. nov., paratype, slide GEL 1135; 18, S. dzieduszyckii (Nowicki), slide Mus. Vind. 11242; 19, S. triglavica Povolný, paratype, slide Mus. Vind. 11241; 20, S. basistrigella Huemer, slide GEL 1139.

FIGURES 1–8 in Cryptic diversity and phylogeography of high alpine Sattleria — a case study combining DNA barcodes and morphology (Lepidoptera: Gelechiidae)

FIGURES 1–8. Adults of Sattleria. 1–2, S. melalecuella (Constant), 1, 3, 2, Ƥ; 3, S. karsholti sp. nov., 3, holotype; 4, S. cottiella sp. nov., 3, holotype; 5, S. izoardi Huemer & Sattler, 3; 6, S. graiaeella sp. nov., 3, holotype; 7, S. basistrigella Huemer, 3; 8, S. triglavica Povolný, 3.

FIGURES 13–16 in Cryptic diversity and phylogeography of high alpine Sattleria — a case study combining DNA barcodes and morphology (Lepidoptera: Gelechiidae)

FIGURES 13–16. Male genitalia of Sattleria. 13, S. pyrenaica (Petry), slide GEL 279; 14, S. cottiella sp. nov., paratype, slide GEL 1144; 15, S. izoardi Huemer, holotype, slide GEL 1145; 16, S. marguareisi Huemer & Sattler, paratype, slide GEL 150.

FIGURES 9–12 in Cryptic diversity and phylogeography of high alpine Sattleria — a case study combining DNA barcodes and morphology (Lepidoptera: Gelechiidae)

FIGURES 9–12. Male genitalia of Sattleria. 9, S. melaleucella (Constant), slide LMP 76; 10, ditto, slide LMP 102; 11, S. arcuata Pitkin & Sattler, paratype, slide GEL 159; 12, S. karsholti sp. nov., paratype, slide LMP 71.

FIGURES 28–31 in Cryptic diversity and phylogeography of high alpine Sattleria — a case study combining DNA barcodes and morphology (Lepidoptera: Gelechiidae)

FIGURES 28–31. Male genitalia of Sattleria (details of valva-vinculum complex). 28, S. cottiella sp. nov., paratype, slide GEL 1144; 29, ditto, paratype, slide GEL 1146; 30, S. izoardi Huemer & Sattler, holotype, slide GEL 1145; 31, ditto, slide GEL 1049.

FIGURES 24–27 in Cryptic diversity and phylogeography of high alpine Sattleria — a case study combining DNA barcodes and morphology (Lepidoptera: Gelechiidae)

FIGURES 24–27. Male genitalia of Sattleria (details of valva-vinculum complex). 24, S. karsholti sp. nov., paratype, slide LMP 74; 25, ditto, paratype, slide LMP 71; 26, S. melalecuella (Constant), slide LMP 76; 27, ditto, slide LMP 102.

FIGURES 21–23 in Cryptic diversity and phylogeography of high alpine Sattleria — a case study combining DNA barcodes and morphology (Lepidoptera: Gelechiidae)

FIGURES 21–23. Male genitalia of Sattleria. 21, S. breviramus Pitkin & Sattler, slide GEL 154; 22, S. stryriaca Pitkin & Sattler, paratype, slide 91/230 P.H.; 23, S. angustispina Pitkin & Sattler, paratype, slide CG 84.

FIGURE 36 in Cryptic diversity and phylogeography of high alpine Sattleria — a case study combining DNA barcodes and morphology (Lepidoptera: Gelechiidae)

FIGURE 36. Neighbour joining tree of the Sattleria (Kimura 2 Parameter) based on sequences of the mtDNA COI gene (barcode fragment 5', 658 bp), except for S. arcuata (307 bp) and S. dzieduszyckii (623 bp).

FIGURE 4 in Parapercis lutevittata, a new cryptic species of Parapercis (Teleostei: Pinguipedidae), from the western Pacific based on morphological evidence and DNA barcoding

FIGURE 4. Comparisons of coloration between (A) Parapercis lutevittata, ASIZP0071627, holotype, 135 mm SL and (B) P. sexfasciata, ASIZP0071631, 142.7 mm SL. a- a large dark-brown blotch and several small black dots vs. a big deep-brown or black blotch on base of pectoral fin; b- absence vs. presence of black spots on base of dorsal fin ray membrane; c- yellow dotted strip midlaterally on body vs. yellow dotted lines from lower left running obliquely to upper right; d- presence vs. absence of scattered black dots on and between V-shaped transverse bands along sides of body.