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Figure 3 from: Korshunova T, Malmberg K, Prkić J, Petani A, Fletcher K, Lundin K, Martynov A (2020) Fine-scale species delimitation: speciation in process and periodic patterns in nudibranch diversity. ZooKeys 917: 15-50. https://doi.org/10.3897/zookeys.917.47444

Figure 3 Periodic-like presentation of colour variation patterns among all species of the genus Amphorina, represented as vertical rows. Three main periods (horizontal rows), each with several subperiods are presented with spotless body/colourless forms at the bottom to forms with a maximal number of spots/coloured body at the top. Note that different species fundamentally display similar colouration patterns, but not all species display all colourations, so some morphs in particular species (e.g., forms with extensive surface pigmentation and dark body in A. farrani, A. linensis, and A. pallida) can either be eventually discovered or do not exist, by some further constraints of the developmental system. Non-observed forms for each particular species are indicated as "unkn" = "unknown"). * = Image from Alder and Hancock 1845.

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Fig. 9 in Phylogenomic Species Delimitation, Taxonomy, and 'Bird Guide' Identification for the Neotropical Ant Genus Rasopone (Hymenoptera: Formicidae)

Fig. 9. Distribution map, face view, and lateral view of petiole of Rasopone costaricensis (holotype worker), R. politognatha (holotype worker), R. JTL035 (worker, Mexico, CASENT0640453), and Rasopone JTL049 (worker, Colombia, CASENT0644557). On distribution maps, red dots are sites with UCE sequence data. Red boxes are type locality.

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Fig. 10 in Phylogenomic Species Delimitation, Taxonomy, and 'Bird Guide' Identification for the Neotropical Ant Genus Rasopone (Hymenoptera: Formicidae)

Fig. 10. Distribution map, face view, and lateral view of petiole of Rasopone costaricensis form b (worker, Costa Rica, INB0003223929), R. JTL047 (worker, French Guiana, CASENT0645960), R. panamensis (worker, Costa Rica, CASENT0644252), and R. subcubitalis (holotype worker). On distribution maps, red dots are sites with UCE sequence data. Red boxes are type locality.

opencc-by-4.0Mar 2020View details →
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Fig. 18 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 18. Phylogeny of the family Clevelandellidae Kidder, 1938 based on 18S rRNA gene sequences. Bootstrap values for maximum likelihood analyses performed in IQTrees and PhyML as well as posterior probabilities for Bayesian interference performed in MrBayes and Phycas were mapped onto the best scoring IQTree. Dashes indicate statistical support below 50%. Sequences obtained in this study are in bold face. Members of each morphospecies are marked by the same color, as shown in Fig. 17. For specimen codes and further details, see Table 6. The scale bar denotes six substitutions per one thousand nucleotide positions.

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Fig. 14. Paraclevelandia brevis Kidder, 1937. Thai I in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 14. Paraclevelandia brevis Kidder, 1937. Thai I specimens (A–C, F–G) isolated from Panesthia angustipennis angustipennis (Illiger, 1801) and Vietnamese specimens (D–E) isolated from Panesthia angustipennis cognata Bey-Bienko, 1969 from life (A–C) and after protargol impregnation (D–G). A. Ventral overview, showing the general body organization. Arrows mark the proximal end of the peristomial opening, black arrowheads denote the proximal end of the adoral zone of membranelles, white arrowheads denote the karyophore attached to the anterior body pole. B–C. Ciliary pattern of ventral and dorsal sides. Asterisks mark the position of the ciliary whorl (posterior suture). D–G. Ventral views of specimens with well-preserved body shape. Scale bars = 20 μm.

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Fig. 13. Paraclevelandia brevis Kidder, 1937. Thai I in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 13. Paraclevelandia brevis Kidder, 1937. Thai I specimens isolated from Panesthia angustipennis angustipennis (Illiger, 1801) after protargol impregnation. A–J. Variability of body shape and size as well as of the nuclear (shaded grey) and oral (shaded yellow) apparatus. Scale bar = 20 μm.

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Fig. 4 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 4. Clevelandella hastula (Kidder, 1937). Vietnamese specimens isolated from Panesthia angustipennis cognata Bey-Bienko, 1969 from life (A) and after protargol impregnation (B–N). A. Ventral view of a representative specimen, length 90 μm. B–K. Variability of body shape and size as well as of the nuclear (shaded grey) and oral (shaded yellow) apparatus. L. Semi-schematic diagram, showing the general body organization. M–N. Ciliary pattern of ventral and dorsal sides. Arrow marks the right suture and black arrowheads denote the position of the ciliary whorl (posterior suture). Scale bars = 30 μm.

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Fig. 2 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 2. Clevelandella constricta (Kidder, 1937). Thai I specimens isolated from Panesthia angustipennis angustipennis (Illiger, 1801) after protargol impregnation. A–J. Variability of body shape and size as well as of the nuclear (shaded grey) and oral (shaded yellow) apparatus. Scale bar = 50 μm.

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Fig. 7 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 7. Clevelandella panesthiae (Kidder, 1937). Vietnamese specimens isolated from Panesthia angustipennis cognata Bey-Bienko, 1969 after protargol impregnation. A–D. Variability of body shape and size as well as of the nuclear (shaded grey) and oral (shaded yellow) apparatus. Scale bar = 50 μm.

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Fig. 17 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 17. Phylogeny of the order Clevelandellida de Puytorac & Grain, 1976 based on 18S rRNA gene sequences. Some metopids were used as outgroup. Bootstrap values for maximum likelihood analyses performed in IQTrees and PhyML as well as posterior probabilities for Bayesian interference performed in MrBayes and Phycas were mapped onto the best scoring IQTree. Dashes indicate statistical support below 50%, asterisks denote mismatch with the topology shown. Sequences obtained in this study are in bold face. Members of each morphospecies are marked by the same color, following the code in the inset. For specimen codes and further details, see Table 6. The scale bar denotes three substitutions per one hundred nucleotide positions.

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Fig. 9 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 9. Clevelandella parapanesthiae (Kidder, 1937). Thai I specimens isolated from Panesthia angustipennis angustipennis (Illiger, 1801) from life (A) and after protargol impregnation (B–N). A. Ventral view of a representative specimen, length 90 μm. B–K. Variability of body shape and size as well as of the nuclear (shaded grey) and oral (shaded yellow) apparatus. L. Semi-schematic diagram, showing the general body organization. M–N. Ciliary pattern of ventral and dorsal sides. Arrow marks the right suture, black arrowheads indicate the position of the ciliary whorl (posterior suture). Scale bars = 30 μm.

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Fig. 12. Paraclevelandia brevis Kidder, 1937 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 12. Paraclevelandia brevis Kidder, 1937. Vietnamese specimens isolated from Panesthia angustipennis cognata Bey-Bienko, 1969 from life (A) and after protargol impregnation (B–N). A. Ventral view of a representative specimen, length 50 μm. B–K. Variability of body shape and size as well as of the nuclear (shaded grey) and oral (shaded yellow) apparatus. L. Semi-schematic diagram, showing the general body organization. M–N. Ciliary pattern of ventral and dorsal sides. Arrow marks the right suture, black arrowheads indicate the position of the ciliary whorl (posterior suture). Scale bars = 20 μm.

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Fig. 6 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 6. Clevelandella panesthiae (Kidder, 1937). Thai I specimens isolated from Panesthia angustipennis angustipennis (Illiger, 1801) from life (A) and after protargol impregnation (B–N). A. Ventral view of a representative specimen, length 105 μm. B–K. Variability of body shape and size as well as of the nuclear (shaded grey) and oral (shaded yellow) apparatus. L. Semi-schematic diagram, showing the general body organization. M–N. Ciliary pattern of ventral and dorsal sides. Arrow marks the right suture, black arrowheads denote the position of the ciliary whorl (posterior suture). Scale bars = 50 μm.

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Fig. 8 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 8. Clevelandella panesthiae (Kidder, 1937). Vietnamese specimens (A, E–G) isolated from Panesthia angustipennis cognata Bey-Bienko, 1969 and Thai I specimens (B–D) isolated from Panesthia angustipennis angustipennis (Illiger, 1801) from life (A, E–G) and after protargol impregnation (B–D). A–D. Ventral view of specimens with well-preserved body shape. Arrow marks the proximal end of the peristomial opening, black arrowheads denote the proximal end of the adoral zone of membranelles, white arrowheads mark the karyophore fibers attaching to the left and right body margins. E. Ventral view, showing the general body organization. F–G. Ciliary pattern of ventral and dorsal sides. Asterisks mark the position of the ciliary whorl (posterior suture), white double arrowhead denotes the right suture. Scale bars = 50 μm.

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Fig. 16 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 16. Ordination analyses of 94 specimens of six clevelandellid morphospecies based on morphometric data (A) and cell shape information (B). A. Multidimensional scaling of the Gower's similarity matrix. B. Principal component analysis of the Fourier coefficients. Eigenvalues of the first two ordination axes are λ1= 0.723 and λ2= 0.135, explaining 85.8% of the total variation. The morphometric and geometrical data delimit the clevelandellid morphospecies consistently and unambiguously, i.e., each clevelandellid morphospecies forms a homogeneous cluster that is well isolated from all other morphospecies analyzed. Members of each morphospecies are highlighted by the same color as in Fig. 15.

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Fig. 15 in Morphological versus molecular delimitation of ciliate species: a case study of the family Clevelandellidae (Protista, Ciliophora, Armophorea)

Fig. 15. Cluster analyses of 94 specimens of six clevelandellid morphospecies based on morphometric data (A) and the cell shape information (B). A. Weighted average linkage clustering method in a combination with Gower's similarity index. B. Ward's D2 clustering method in a combination with the Manhattan city block distance. The morphometric and geometrical data delimit the clevelandellid morphospecies consistently and unambiguously, i.e., each clevelandellid morphospecies forms a homogeneous cluster that is well isolated from all other morphospecies analyzed. Since both techniques are phenetic, their results refer only to the similarity of the analyzed morphospecies in phenotypic space and not to their relatedness. Members of each morphospecies are highlighted by the same color.

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Figure 2 from: Monjardim M, Azevedo CO, Fagundes V (2020) DNA barcoding and hypopygium shape support delimitation of sympatric Dissomphalus species (Hymenoptera, Bethylidae) from the Atlantic rainforest. ZooKeys 959: 87-97. https://doi.org/10.3897/zookeys.959.53737

Figure 2 A bayesian consensus tree generated from the 304-bp COI from 29 representatives of the species complex. Posterior probabilities (PP) and bootstrap (BT) indicated above branches. The species D. thaianus, D. wusheanus and D. chiangmaiensis were used as outgroups to root the tree B–D hypopygium magnified 9.2×, corresponding to each clade.

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Figure 1 from: Monjardim M, Azevedo CO, Fagundes V (2020) DNA barcoding and hypopygium shape support delimitation of sympatric Dissomphalus species (Hymenoptera, Bethylidae) from the Atlantic rainforest. ZooKeys 959: 87-97. https://doi.org/10.3897/zookeys.959.53737

Figure 1 Locations of the samples in Brazil and Paraguay (see Appendix I for geographic coordinates).

opencc-by-4.0Aug 2020View details →
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Data from: Transcriptome analysis of two radiated Cycas species and its utilization on species delimitation in Cycas taiwaniana complex

Premise of the study: Cycas is an important gymnosperm with the most diverse of all cycad genera. The taxa within Cycas taiwaniana complex are morphologically similar and difficult to be distinguished by a lack of genomic resources. Methods: We characterized transcriptomes of two closely related and endangered Cycas species endemic to Hainan, China: C. hainanensis and C. changjiangensis. Furthermore, we sequenced three single copy nuclear genes for the Cycas taiwaniana complex developed from transcriptome. Then we evaluated species boundaries based on the multispecies coalescent method implemented in BPP. Results: We obtained 68,184 and 81,561 unigenes for C. changjiangensis and C. hainanensis, respectively. The estimated divergence time revealed the two related species diverged more recently. Six positively selected genes are mainly involved in stimulus responses, suggesting that environmental adaptation may play an important role in the divergence of the two species. The similar peak at 1.0 of Ks distributions for paralogs indicated a common whole-genome duplication event. Results of species delimitation indicated the Cycas taiwaniana complex consisted of three distinct lineages, which corresponds to morphological differentiation. Discussion: Our study provides evidence from transcriptome for taxonomical treatment of the Cycas taiwaniana complex and new insights into evolution of this living fossil genus.

opencc-zeroAug 2020View details →
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a from: Ling S-J, Qin X-T, Song X-Q, Zhang L-N, Ren M-X (2020) Genetic delimitation of Oreocharis species from Hainan Island. In: Shui Y-M, Chen W-H, Ren M-X, Wen F, Hong X, Qiu Z-J, Wei Y-G, Kang M (Eds) Taxonomy of Gesneriaceae in China and Vietnam. PhytoKeys 157: 59-81. https://doi.org/10.3897/phytokeys.157.32427

a The results graph of the relationship between genetic and geographical distance for 12 populations based on the (a) ITS1/2 and (b) cpDNA

opencc-by-4.0Sep 2020View details →

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