Find research datasets worth reusing

opencc-by-4.0Dec 2022View details →

Table 1 in Perinatal exposure to a high-fat diet alters proopiomelanocortin, neuropeptide Y and dopaminergic receptors gene expression and the food preference in offspring adult rats

Table 1. Experimental design. The rats were fed commercial standard diet for rodents control diet or high-fat diet during pregnancy, lactation and post-weaning (up to 100 days of life).During the feeding period, rats consumed both diets (control diet and high-fat diet). The numbers in parentheses indicate the animals number in each nutritional group.<table><tbody><tr><th>Experimental Design</th></tr></tbody><tbody><tr><th>Genitors (n)</th><td>Gestation and lactation</td><td>Offspring during lactation (n)</td><td>Offspring (n)</td><td>Post-weaning (21-100° life’s day)</td><td>Food preference (102-116º life’s day)</td></tr><tr><th></th><td></td><td></td><td>CC (12)</td><td>Control diet</td><td>Control diet</td></tr><tr><th></th><td></td><td></td><td></td><td></td><td>High-fat diet</td></tr><tr><th>GC (5)</th><td>Control diet</td><td>C (23)</td><td>CH (11)</td><td>High-fat diet</td><td>Control diet</td></tr><tr><th></th><td></td><td></td><td></td><td></td><td>High-fat diet</td></tr><tr><th></th><td></td><td></td><td>HC (11)</td><td>Control diet</td><td>Control diet</td></tr><tr><th></th><td></td><td></td><td></td><td></td><td>High-fat diet</td></tr><tr><th>GH (5)</th><td>High-fat diet</td><td>H (23)</td><td>HH (12)</td><td>High-fat diet</td><td>Control diet</td></tr><tr><th></th><td></td><td></td><td></td><td></td><td>High-fat diet</td></tr></tbody></table>

opencc-by-4.0Sep 2024View details →

Evaluating rapid water excitation techniques for 5D whole-heart fat-suppressed free-running cardiac MRI at 1.5T scanner

Volunteer dataset of the paper: "Evaluating Rapid Water Excitation Techniques for 5D Whole-Heart Fat-Suppressed Free-Running Cardiac MRI at 1.5T Scanner" The folder "InVivo" contains raw data from five different volunteers, with each volunteer having undergone 5 different acquisitions: bSSFP, FISS, BORR, LIBRE, and LIBOR.

opencc-by-4.0Oct 2024View details →

Repeated autologous fat grafting significantly increases mas-tectomy flap thickness in prepectoral multi-stage composite expander-to-implant breast reconstruction: exploring the con-cept of a reverse expansion

Supplementary material 1 (Video): MRI sequences of a 37-year-old (A), 44-year-old (B) and 46-year-old (C) patient after bilateral mastectomy, sparing both the skin and nipple-areolar com-plexes, with prepectoral insertion of a breast tissue expander. These sequences were captured be-fore repeated sessions of autologous fat grafting to the mastectomy flap and reverse expansion of the expander. In D, E, and F, the corresponding MRI sequences were performed 3 months after 3 sessions of autologous fat grafting each and the exchange of the expander with a definitive im-plant. Supplementary material 2 (Video): Repeated contraction of bilateral pectoralis major muscles in a 37-year-old (A), 44-year-old (B) and 46-year-old (C) patient after bilateral mastectomy sparing both the skin and nipple-areolar complexes, with prepectoral expander-to-implant breast reconstruction and repeated autologous fat grafting sessions while gradually deflating the ex-pander. Note the almost complete absence of breast animation, even in the 44-year-old patient having the implant stabilized with a synthetic, non-absorbable mesh to prevent caudal “bottom-ing out” and lateral migration.

opencc-by-4.0Mar 2020View details →

Dataset for "Plasma biochemistry, gene expression and liver histomorphology in common carp (Cyprinus carpio) fed with different dietary fat sources. Food and Chemical Toxicology, 139, 111300"

Dataset for "Eljasik P, Panicz R, Sobczak M, Sadowski J, Barbosa V, Marques A, Dias J. (2020). Plasma biochemistry, gene expression and liver histomorphology in common carp (Cyprinus carpio) fed with different dietary fat sources. Food and Chemical Toxicology, 139, 111300. DOI:<a href="https://doi.org/10.1016/j.fct.2020.111300"> doi.org/10.1016/j.fct.2020.111300</a>"

opencc-by-4.0Dec 2021View details →

Neuronal HSF-1 coordinates the propagation of fat desaturation across tissues to enable adaptation to high temperatures in C. elegans

To survive elevated temperatures, ectotherms adjust the fluidity of membranes by fine-tuning lipid desaturation levels in a process previously described to be cell-autonomous. We have discovered that, in Caenorhabditis elegans, neuronal Heat shock Factor 1 (HSF-1), the conserved master regulator of the heat shock response (HSR)- causes extensive fat remodelling in peripheral tissues. These changes include a decrease in fat desaturase and acid lipase expression in the intestine, and a global shift in the saturation levels of plasma membrane’s phospholipids. The observed remodelling of plasma membrane is in line with ectothermic adaptive responses and gives worms a cumulative advantage to warm temperatures. We have determined that at least six TAX-2/TAX-4 cGMP gated channel expressing sensory neurons and TGF-β/BMP are required for signalling across tissues to modulate fat desaturation. We also find neuronal hsf-1  is not only sufficient but also partially necessary to control the fat remodelling response and for survival at warm temperatures. This is the first study to show that a thermostat-based mechanism can cell non-autonomously coordinate membrane saturation and composition across tissues in a multicellular animal.

opencc-zeroOct 2021View details →

Changes in fat mass and migratory restlessness in northern wheatears

To fuel their migratory endurance flights, most birds accumulate large quantities of fat prior to departure. It therefore seems logical that the decision to depart on a migratory flight depends on fuel stores, at least to some extent; very small fuel stores prohibit migratory endurance flight. However, studies linking migrants' estimated fuel stores (or sometimes actual fat stores) to departure likelihood have provided inconsistent results. Fuel stores are not static though, but highly dynamic. Instead of investigating fuel stores at a given time, we therefore focussed on quantifying the dynamic process of fuelling and how it affects migrants' motivation to depart. We did so by temporarily caging wild northern wheatears (Oenanthe oenanthe) at a stopover site during autumn migration. Birds were kept under controlled conditions, receiving ad libitum food. Changes in fat mass (measured by quantitative magnetic resonance) and migratory restlessness (an accurate proxy for migrants' motivation to depart from stopover) were closely monitored. We found that the change in fat mass was a good predictor of the change in migratory restlessness. Individuals accumulating little fat did not show much change in their restlessness, while individuals accumulating much fat showed a strong increase in restlessness. Our study therewith provides clear support for the idea that fat stores affect birds' motivation to migrate, and shows that for migrating birds, increasing fat stores are an important cue to depart from stopover. Fat accumulation affected the change in migratory restlessness in the same manner for relatively lean and relatively fat birds. Additionally, food intake or changes therein did not explain the change in migratory restlessness we observed. We therefore hypothesize that the fat tissue itself signals changes in energetic state, likely through production and release of hormones, affecting birds' motivation to migrate.

opencc-by-4.0Dec 2022View details →

Borealization of Arctic zooplankton—smaller and less fat zooplankton species in Disko Bay, Western Greenland

Data for chlorophyll a and copepods in Figure 2 in the publication, doi: 10.1002/lno.11380

opencc-zeroJan 2023View details →

Data of multiple different high-fat diets

Background and Objectives: The spectrum of non-alcoholic fatty liver disease (NAFLD), known as non-alcoholic steatohepatitis (NASH), can lead to advanced liver disease. It is known that a variety of diets play a significant role in the development of NAFLD/NASH. The goal of this study is to determine the most appropriate composition of diet to induce NASH in an animal model. Methods: This research used Rattus norvegicus strain Wistar (n=27), which were divided into four groups and given each diet for 12 weeks: normal diet (ND, n=7), high-fat diet (HFD, n=6), Western diet (WD, n=7), high-fat-high-fructose diet (HFHFD, n=7). Subjects were documented for body weight. Blood samples were taken for biochemical analysis: low-density lipoprotein (LDL), triglyceride, alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), hepatic lipase, tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and lipopolysaccharide (LPS). Feces were taken for short-chain fatty acid (SCFA) analysis. Liver histology was analyzed using NAS (NAFLD activity score). The comparison test was carried out using the one-way ANOVA or Kruskal–Wallis test. Results: The highest mean of body weight was in the WD group (346.14 g). Liver enzymes, LDL, triglyceride, propionic acid, and acetic acid in each group were not significantly different. TNF-α, IL-6, and hepatic lipase were significant (p = 0.000; p = 0.000; p = 0.004) and the highest was in the HFD group. Butyrate level was significant (p = 0.021) and the least was in the HFHFD group (4.77 mMol/g). Only WD and HFHFD had an NAS ≥ 5 (14% and 14%). The highest percentage of borderline NAS was found in WD (57%). Conclusions: The HFD group induced significant liver inflammation but did not produce NASH histologically, whereas the WD and HFHFD groups had the potential to develop NASH because the diets affected metabolic and inflammatory parameters as well as liver histology.

opencc-zeroMar 2023View details →

Dietary fat supplements influence weight gain and egg production but not offspring sex ratios in Japanese quail

Sex ratio theory suggests that females should bias offspring sex ratios based on maternal condition and the availability of critical food resources. Work in birds indicates that females do, indeed, bias sex ratios according to maternal condition and food quality and quantity. Yet it is unknown whether these sex ratio skews occur due to fluctuations in particular micro- or macro-nutrients, caloric content overall, or even the perception of food availability. We hypothesized that dietary fats may drive biases in offspring sex ratios, because measures of maternal condition often reflect fat reserves, and fats are critical for the process of egg-laying in birds. To test this, we provided breeding Japanese quail, a species that biases sex ratios in response to maternal condition, with either a control breeding diet or a diet supplemented with two oils (safflower oil and flaxseed oil). These oils were chosen for their high omega-3 and omega-6 fatty acid content as well as their importance in mammalian sex allocation. We then measured influences of these diets on the sex ratio of offspring, the change in maternal weight, and the laying rates of female quail. The dietary oil supplements increased weight gains in quail but decreased the number of eggs laid during the experiment. There was no influence of the oil supplements on offspring sex ratios. This indicates that fat may not be a macro-nutrient involved in the process of sex ratio adjustment in quail.

opencc-zeroMay 2023View details →

Data from: The fat body cortical actin network regulates Drosophila inter-organ nutrient trafficking, signaling, and adipocyte cell size

Defective nutrient storage and adipocyte enlargement (hypertrophy) are emerging features of metabolic syndrome and type 2 diabetes. How the cytoskeletal network contributes to nutrient uptake, fat storage, and adipocyte size remains poorly understood. Utilizing the Drosophila larval fat body (FB) as a model adipose tissue, we show that a specific actin isoform—Act5C—forms the cortical actin network necessary for inter-organ lipid trafficking. Act5C also promotes FB tissue expansion during larval development so larvae can store sufficient biomass for metamorphosis. We find FB-specific loss of Act5C, but not other Drosophila actins, perturbs FB triglyceride (TG) storage in lipid droplets (LDs), resulting in developmentally delayed larvae that fail to develop into flies. Act5C localizes to the FB cell surface where it intimately contacts peripheral LDs (pLDs), forming a cortical actin network together with spectrins for cell architectural support. While both the cortical actin and spectrin cytoskeletons maintain FB cell surface architecture, we find that only the actin network is required for fat storage. Mechanistically, we show that FBs lacking the Act5C cortical cytoskeleton exhibit a block in lipoprotein (Lpp) secretion from FB cells, and a subsequent disruption of gut:FB inter-organ lipid transport, resulting in mid-gut fat accumulation. Utilizing temporal RNAi-depletion approaches, we also reveal that Act5C is indispensable post-embryogenesis during larval feeding to promote FB cell expansion. Act5C-deficient FBs fail to expand cell sizes, leading to lipodystrophic larvae unable to accrue sufficient biomass for metamorphosis. Collectively, we propose that the Act5C-mediated cortical actin network of Drosophila adipose tissue plays an essential role in post-embryonic inter-organ nutrient transport and FB cell size determination for organismal energy homeostasis and development.

opencc-by-4.0Jun 2023View details →

Corticosterone treatment results in fat deposition and body mass maintenance without effects on feeding behaviour or immunity in female lizards (Tropidurus catalanensis)

In different life history stages, animals must maintain homeostasis through predictable, unpredictable and/or challenging events. Glucocorticoids (GC), hormones released in response to hypothalamus-pituitary-adrenal/interrenal (HPA/HPI) axis activation, promote various behavioural and physiological adjustments on a daily manner; and in order to restore balance, after facing stressors. When GC are elevated for an extended period and in high concentrations, characterizing a chronic exposure, it can lead to deleterious effects on animals’ physiology, such as accumulation of fat bodies, changes in feeding behaviour and suppression of immune function. We aim to elucidate the effects of chronic corticosterone exposure and body condition index (BI) in fat deposition, feeding behaviour and immune function in Tropidurus catalanensis’ females. Thirty animals were divided in three groups: 1. Control (no experimental procedure was performed); 2. Empty Implant (animals surgically received an empty silastic tube); and 3. CORT Implant (animals surgically received one silastic tube filled with CORT). Blood samples were collected throughout the experiment to assess CORT plasma levels, total and differential leukocyte count, bacterial killing ability (BKA), and hemagglutination titer. An immune challenge using phytohemagglutinin (PHA) was conducted to measure innate and adaptive immune response. Feeding behaviour and fat bodies were also evaluated. After implantation, CORT treated animals maintained a stable body mass through weeks of captivity, while Control and Empty Implant groups displayed weight loss. In the CORT treated animals, there was also a positive relation between BI and fat bodies, and higher fat bodies deposition when compared to groups 1 and 2. No effects of CORT treatment were observed on immune response or feeding behaviour.

opencc-by-4.0Jun 2023View details →

Gut microbe compositions in High Fat diet

This study aimed to explore the relationship between the gut microbiome and liver function, with the objective of identifying a specific bacterial species that may exhibit beneficial effects in improving the symptoms of NAFLD. We conducted a comparative analysis of the gut microbiome composition between mice fed a normal chow (NC) diet and mice fed a high-fat (HF) diet in order to identify specific gut microbes. The consumption of a high-fat diet led to a decrease in the prevalence of Kineothrix bacteria. To assess the effects of Kineothrix on liver health, we conducted a comparative analysis of the gut microbiome composition between mice fed a diet high in fat and fructose (HFHF), with and without the administration of Kineothrix.

opencc-zeroSep 2023View details →

Association of fat-to-muscle ratio with non-alcoholic fatty liver disease: a single-centre retrospective study

Objectives: Sarcopenia is a known risk factor for non-alcoholic fatty liver disease (NAFLD). Studies evaluating the association between the fat-to-muscle ratio (FMR) and NAFLD are limited. Therefore, the aim of our study was to investigate the association between FMR and NAFLD. Design: A retrospective study was conducted on the individuals who underwent health examination in Wuhan Union Hospital between January 2020 and November 2021. Clinical data were collected from electronic medical records. Setting: Our study was conducted in a hospital in China. Participants: A total of 1,592 participants aged ≥40 years who underwent body composition analysis and liver ultrasonography were retrospectively reviewed. Primary outcome measures: Liver ultrasonography was used to assess liver steatosis, and the Fibrosis-4 Index (FIB-4) was used to calculate the risk scores for liver fibrosis. The 10-year atherosclerotic cardiovascular disease (ASCVD) risk prediction model was used to calculate ASCVD risk scores. Results: The FMR was significantly higher in individuals with NAFLD than in those without NAFLD (P<0.001). The prevalence of NAFLD gradually increased from FMR tertile 1 (reference) to tertile 2 (OR=1.49, 95% CI: 1.13–1.97) and tertile 3 (OR=2.85, 95% CI: 2.08–3.90). In addition, patients with NAFLD in FMR tertile 3 had a significantly higher risk of liver fibrosis (OR=4.48, 95% CI: 2.12–9.50) and ASCVD (OR=4.63, 95% CI: 2.62–8.19) than those in FMR tertile 1 after adjustment for multiple confounders. Conclusion: In this study, we found a significant association between FMR and NAFLD. A higher FMR indicates a higher risk of NAFLD in the study population and a higher risk of liver fibrosis and ASCVD in NAFLD patients.

Reduced Carbohydrate Versus Fat in Obese Subjects

ClinicalTrials.gov study NCT00846040. IPD Sharing: Not stated. Countries: 1. Publications: 8.

Serial Assessment of Body Fat Accrual in Very Preterm Infants

ClinicalTrials.gov study NCT03575897. IPD Sharing: Not stated. Countries: 1. Publications: 1.

A Double-blind Randomized Placebo-controlled Study Comparing Epanova and Fenofibrate on Liver Fat in Overweight Subjects.

ClinicalTrials.gov study NCT02354976. IPD Sharing: Not stated. Countries: 1. Publications: 1.

Changes in Triglyceride and Other Lipids (Levels of Fats Found in Blood) When Taking Darunavir Compared to Atazanavir in HIV-infected Patients That Have Never Received Treatment

ClinicalTrials.gov study NCT00757783. IPD Sharing: Not stated. Countries: 1. Publications: 2.

closedIPD-NOFeb 2026View details →

Autologous Fat Grafting Beneath Penile Split Thickness Skin Graft Placement During Penile Reconstruction

ClinicalTrials.gov study NCT07316491. IPD Sharing: NO. Countries: 1. Publications: 17.

Open-Label Study of Deoxycholic Acid for the Reduction of Localized Subcutaneous Fat in the Submental Area

ClinicalTrials.gov study NCT01426373. IPD Sharing: Not stated. Countries: 1. Publications: 1.

Effect of Caloric Restriction on Fat Oxidation in Obese Men and Women (Magellan II)

ClinicalTrials.gov study NCT01616082. IPD Sharing: Not stated. Countries: 1. Publications: 19.