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595 results for “High-throughput sequencing”
Figure 2 from: Siddique AB, Khokon AM, Unterseher M (2017) What do we learn from cultures in the omics age? High-throughput sequencing and cultivation of leaf-inhabiting endophytes from beech (Fagus sylvatica L.) revealed complementary community composition but similar correlations with local habitat conditions. MycoKeys 20: 1-16. https://doi.org/10.3897/mycokeys.20.11265
Figure 2 - . Principal coordinate analysis (PCoA) of fungal leaf-inhabiting endophytes of beech display strongly differing assemblages obtained with Illumina sequencing and cultivation. Both methods revealed differing mycobiomes from valley and from mountain leaves, although these differences were less pronounced for cultivation data. Abbreviations: IM = Illumina data from mountain samples, IV = Illumina data from valley samples, CM = cultivation data from mountain samples, CV = cultivation data from valley samples
Figure 3 from: Siddique AB, Khokon AM, Unterseher M (2017) What do we learn from cultures in the omics age? High-throughput sequencing and cultivation of leaf-inhabiting endophytes from beech (Fagus sylvatica L.) revealed complementary community composition but similar correlations with local habitat conditions. MycoKeys 20: 1-16. https://doi.org/10.3897/mycokeys.20.11265
Figure 3 - Abundance distribution of the 20 most abundant orders of fungal leaf-inhabiting endophytes of beech on a logarithmic scale. Three of the five most abundant orders from high-throughput sequencing were also most abundant in cultivation data.
Figure 1 from: Siddique AB, Khokon AM, Unterseher M (2017) What do we learn from cultures in the omics age? High-throughput sequencing and cultivation of leaf-inhabiting endophytes from beech (Fagus sylvatica L.) revealed complementary community composition but similar correlations with local habitat conditions. MycoKeys 20: 1-16. https://doi.org/10.3897/mycokeys.20.11265
Figure 1 - Diversity indexes and accumulation curves for a Illumina and b cultivation data of fungal leaf-inhabiting endophytes of beech. Except of the accumulation curves of cultivation data, both methods revealed a clear and partly significant trend of higher fungal diversity at the valley site.
Supplementary material 3 from: Lefort M, Wratten S, Cusumano A, Varennes Y, Boyer S (2017) Disentangling higher trophic level interactions in the cabbage aphid food web using high-throughput DNA sequencing. Metabarcoding and Metagenomics 1: e13709. https://doi.org/10.3897/mbmg.1.13709
Exploratory statistics addressing sequencing depth per country and MOTU rarefaction.
Supplementary material 2 from: Lefort M, Wratten S, Cusumano A, Varennes Y, Boyer S (2017) Disentangling higher trophic level interactions in the cabbage aphid food web using high-throughput DNA sequencing. Metabarcoding and Metagenomics 1: e13709. https://doi.org/10.3897/mbmg.1.13709
Supporting Information 2
Supplementary material 10 from: Ushio M, Murakami H, Masuda R, Sado T, Miya M, Sakurai S, Yamanaka H, Minamoto T, Kondoh M (2018) Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing. Metabarcoding and Metagenomics 2: e23297. https://doi.org/10.3897/mbmg.2.23297
Abundance of some common fish species obtained by the direct visual census
Supplementary material 2 from: Ushio M, Murakami H, Masuda R, Sado T, Miya M, Sakurai S, Yamanaka H, Minamoto T, Kondoh M (2018) Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing. Metabarcoding and Metagenomics 2: e23297. https://doi.org/10.3897/mbmg.2.23297
Primer, index and probe sequences used in the study
Supplementary material 1 from: Ushio M, Murakami H, Masuda R, Sado T, Miya M, Sakurai S, Yamanaka H, Minamoto T, Kondoh M (2018) Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing. Metabarcoding and Metagenomics 2: e23297. https://doi.org/10.3897/mbmg.2.23297
The numbers of sequence reads remaining (filtered) in data processing steps
Supplementary material 7 from: Ushio M, Murakami H, Masuda R, Sado T, Miya M, Sakurai S, Yamanaka H, Minamoto T, Kondoh M (2018) Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing. Metabarcoding and Metagenomics 2: e23297. https://doi.org/10.3897/mbmg.2.23297
Results of quantitative PCR for total fish eDNA, Japanese anchovy and Japanese jack mackerel
Supplementary material 3 from: Ushio M, Murakami H, Masuda R, Sado T, Miya M, Sakurai S, Yamanaka H, Minamoto T, Kondoh M (2018) Quantitative monitoring of multispecies fish environmental DNA using high-throughput sequencing. Metabarcoding and Metagenomics 2: e23297. https://doi.org/10.3897/mbmg.2.23297
Descriptions of TaqMan probe specificity test and supplementary Table S1 and S2
Figure 3 from: Anslan S, Nilsson RH, Wurzbacher C, Baldrian P, Tedersoo L, Bahram M (2018) Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding. MycoKeys 39: 29-40. https://doi.org/10.3897/mycokeys.39.28109
Figure 3 - Number of OTUs per sample for Illumina data recorded from a) pipeline-generated OTU tables (median differences = 38 OTUs) and from b) filtered OTU tables (median differences = 12 OTUs). The Galaxy workflow was excluded here.
Figure 2 from: Anslan S, Nilsson RH, Wurzbacher C, Baldrian P, Tedersoo L, Bahram M (2018) Great differences in performance and outcome of high-throughput sequencing data analysis platforms for fungal metabarcoding. MycoKeys 39: 29-40. https://doi.org/10.3897/mycokeys.39.28109
Figure 2 - OTU accumulation curves of the evaluated pipelines for a) PacBio and b) Illumina datasets.
Supplementary material 2 from: Vohník M, Borovec O, Kolaříková Z, Sudová R, Réblová M (2019) Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica. MycoKeys 55: 59-86. https://doi.org/10.3897/mycokeys.55.35682
: Data type: species data
Figure 6 from: Vohník M, Borovec O, Kolaříková Z, Sudová R, Réblová M (2019) Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica. MycoKeys 55: 59-86. https://doi.org/10.3897/mycokeys.55.35682
Figure 6 Colonial morphotypes of Posidoniomycesatricolor in vitro (type isolate BRK-21). a Compact morphotype with substrate mycelium b, d compact colonies with a cerebriform pattern c colony of P.atricolor on PCA e rhizoidal and compact (arrow) daughter colonies on PCA washed with sterile tap water f detail of the colonies encircled in e; g, h terminal capitate swellings on the surface of compact colonies i–k conspicuous swellings on aerial mycelium. Scale bars: 500 μm (a, d), 1000 μm (b, c), 5 mm (e), 200 μm (f), 100 μm (g), 20 μm (h).
Figure 5 from: Vohník M, Borovec O, Kolaříková Z, Sudová R, Réblová M (2019) Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica. MycoKeys 55: 59-86. https://doi.org/10.3897/mycokeys.55.35682
Figure 5 In vivo root colonisation pattern and in vitro cultural aspects of Posidoniomycesatricolor. a In vivo colonisation on the root surface (arrows) and in the hypodermis (asterisks) of P.oceanicabDSE colonisation on the root surface c germinating microsclerotia stained with trypan blue (arrows) d compact colony developed from microsclerotia (arrow) e surface-sterilised root segments yielding P.atricolor compact colonies (black arrows), sometimes with substrate mycelium (white arrows) f compact colonial morphotype g mycelial colonial morphotype h mycelial morphotype developing from microsclerotia (arrows) in transversal section. Scale bars: 20 μm (a, b), 50 μm (c), 100 μm (d), 200 μm (f, h), 500 μm (g).
Figure 2 from: Vohník M, Borovec O, Kolaříková Z, Sudová R, Réblová M (2019) Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica. MycoKeys 55: 59-86. https://doi.org/10.3897/mycokeys.55.35682
Figure 2 Map of the Mediterranean Sea with location of our 32 sampling sites. For further details see Table 1.
Figure 4 from: Vohník M, Borovec O, Kolaříková Z, Sudová R, Réblová M (2019) Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica. MycoKeys 55: 59-86. https://doi.org/10.3897/mycokeys.55.35682
Figure 4 Phylogram and map showing a distribution pattern of Posidoniomycesatricolor. a Phylogram generated from maximum likelihood analysis based on ITS sequence data for Posidoniomycesatricolor and representatives of the Aigialaceaeb map of the Mediterranean Sea with our 32 sampling sites. Sites in blue, orange, violet and green colour indicate locations of P.atricolor strains with corresponding mutations in ITS2 sequences.
Figure 3 from: Vohník M, Borovec O, Kolaříková Z, Sudová R, Réblová M (2019) Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica. MycoKeys 55: 59-86. https://doi.org/10.3897/mycokeys.55.35682
Figure 3 Phylogram generated from maximum likelihood analysis based on combined nucLSU, nucSSU and RPB2 sequence data for Posidoniomycesatricolor and the Aigialaceae. Species names given in bold are type species. The ex-type of the taxonomic novelty is in bold and blue. An asterisk (*) indicates branches with ML BS = 100% and PP values = 1.0. Branch support of nodes ≥ 70 % ML BS and ≥ 0.90 PP is indicated above or below branches.
Figure 1 from: Vohník M, Borovec O, Kolaříková Z, Sudová R, Réblová M (2019) Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica. MycoKeys 55: 59-86. https://doi.org/10.3897/mycokeys.55.35682
Figure 1 The dominant Mediterranean seagrass Posidoniaoceanica. a Overall appearance, note dense branched root system of the seagrass (encircled) bPosidoniaoceanica growing on an approx. 1.5 m thick layer of matte c typical habitat of the dominant Mediterranean seagrass, note the layer of shed seagrass leaves on the seabed.
Supplementary material 1 from: Vohník M, Borovec O, Kolaříková Z, Sudová R, Réblová M (2019) Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. (Aigialaceae, Pleosporales) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica. MycoKeys 55: 59-86. https://doi.org/10.3897/mycokeys.55.35682
: Data type: species data
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OpenNeuro
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