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242 results for “Microfluidics”
MFMET webinar - 06. Measuring the dimensions of microfluidic devices using optical methods
<p>This video presents optical methods for measurements of dimensions (for example width, height, and length) of microfluidic devices and microchannels. Among the presented methods are optical microscopy and confocal laser scanning microscopy.</p> <p> </p> <p>Furter reading can be found:</p> <p>https://mfmet.eu/publications</p> <p>Yin et al. (2024). MFMET A3.3.4 Report on the geometry and dimension of microfluidic component ports. <a href="https://doi.org/10.5281/zenodo.10848484">Link</a></p> <p>Büker et al. (2024). MFMET Deliverable 8: Measurement protocols for dimensional characterisation of microfluidic components. <a href="https://doi.org/10.5281/zenodo.11098276">Link</a></p> <p> </p> <p>The project (20NRM02 MFMET) have received funding from the EMPIR programme co-financed by the Participating States and from the European Union’s Horizon 2020 research and innovation programme.</p>
MFMET webinar - 05. Interfacing of microfluidic devices
<p>All microfluidic devices need a connection to the outside macroscopic world, e.g. for samples or reagents. This video presents information of and requirements for connectors/interfaces in microfluidics.</p> <p> </p> <p>Furter reading can be found:</p> <p>https://mfmet.eu/publications</p> <p>MFMET Deliverable 5 - Guidelines for the measurement of key performance parameters of microfluidic connections including the identification of key properties in an interface. Zenodo. <a href="https://doi.org/10.5281/zenodo.10731759">Link</a></p> <p>MFMET A3.1.4 White paper on common microfluidic component materials. Zenodo. <a href="https://doi.org/10.5281/zenodo.7930231">Link</a></p> <p><br><br>The project (20NRM02 MFMET) have received funding from the EMPIR programme co-financed by the Participating States and from the European Union’s Horizon 2020 research and innovation programme.</p>
A fluid-walled microfluidic platform for human neuron microcircuits and directed axotomy
<p><strong>ABSTRACT</strong></p> <div> <p>In our brains, different neurons make appropriate connections; however, there remain few in vitro models of such circuits. We use an open microfluidic approach to build and study neuronal circuits in vitro in ways that fit easily into existing bio-medical workflows. Dumbbell-shaped circuits are built in minutes in standard Petri dishes; the aqueous phase is confined by fluid walls – interfaces between cell-growth medium and an immiscible fluorocarbon, FC40. Conditions are established that ensure post-mitotic neurons derived from human induced pluripotent stem cells (iPSCs) plated in one chamber of a dumbbell remain where deposited. After seeding cortical neurons on one side, axons grow through the connecting conduit to ramify amongst striatal neurons on the other – an arrangement mimicking unidirectional cortico-striatal connectivity. We also develop a moderate-throughput non-contact axotomy assay. Cortical axons in conduits are severed by a media jet; then, brain-derived neurotrophic factor and striatal neurons in distal chambers promote axon regeneration. As additional conduits and chambers are easily added, this opens up the possibility of mimicking complex neuronal networks, and screening drugs for their effects on connectivity.</p> <p> </p> </div> <p><strong>FILE DESCRIPTIONS</strong></p> <p>Source Data.xlsx: Tabular datasets plotted on Main Figures 2, 3 and 6, and Supplementary Figures 1 and 2.</p> <p>Key Resources Table.xlsx: Table containing details on the key resources (antibodies, cell lines, virus strains, software, code, and protocols) used in this study.</p>
A novel microfluidic tool for the evaluation of local drug delivery systems in simulated in vivo conditions
Open the record for dataset details and reuse information.
Microfluidic device for real-time formulation of reagents and their subsequent encapsulation into double emulsions
<p>Emulsion drops are often employed as picoliter-sized containers to perform screening assays. These assays usually entail the formation of drops encompassing discrete objects such as cells or microparticles and reagents to study interactions between the different encapsulants. Drops are also used to screen influences of reagent concentrations on the final product. However, these latter assays are less frequently performed because it is difficult to change the reagent concentration over a wide range and with high precision within a single experiment. In this paper, we present a microfluidic double emulsion drop maker containing pneumatic valves that enable real-time formulation of different reagents using pulse width modulation and consequent encapsulation of the mixed solutions. This device can produce drops from reagent volumes as low as 10 µL with minimal sample loss, thereby enabling experiments that would be prohibitively expensive using drop generators that do not contain valves. We employ this device to monitor the kinetics of the cell-free synthesis of green fluorescent proteins inside double emulsions. To demonstrate the potential of this device for real-time formulation, we perform DNA titration experiments to test the influence of DNA concentration on the amount of green fluorescence protein produced in double emulsions by a coupled cell-free transcription / translation system.</p>
Data for: An Imaging Scheme to Study Chaotic Flow in Co-Flow Microfluidics: Implications for Nanoprecipitation
<p>This dataset includes the raw data and analysis of the mixing flow of water and ethanol in microfluidics device using imaging approach.</p>
Data for "Wettability alteration of microfluidic devices by in situ plasma"
<p>Here are the data associated with the paper entitled "Wettability alteration of microfluidic devices by in situ plasma": measurements of contact angle (varying with treatment time, and with aging), and XPS spectra. </p>
supplement videos of Solution for Mass Production of High-Throughput Digital Microfluidic Chip Based on Thin Film Transistor Technology
<p>supplement videos of Solution for Mass Production of High-Throughput Digital Microfluidic Chip Based on Thin Film Transistor Technology</p>
Microfluidic device videos of malaria infected red blood cells
<p>These videos contain malaria-infected red blood cells moving through microfluidic devices.</p>
Data: Thermophoretic microfluidic cells for evaluating Soret coefficient of colloidal particles
<p>Data, which are presented in the following publication: Lee, Namkyu; Mohanakumar, Shilpa; Wiegand, Simone (2022): Thermophoretic microfluidic cells for evaluating Soret coefficient of colloidal particles. In: Int J Heat Mass Tran 194, S. 123002. DOI: 10.1016/j.ijheatmasstransfer.2022.123002.</p>
Source data for "Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting".
<p>Contains numeric data for figures 16-18 and supplementary fig 6 linked with the publication: Panwar, J., Autour, A. & Merten, C.A. Design and construction of a microfluidics workstation for high-throughput multi-wavelength fluorescence and transmittance activated droplet analysis and sorting. <em>Nat Protoc</em> (2023). <a href="https://doi.org/10.1038/s41596-022-00796-2">https://doi.org/10.1038/s41596-022-00796-2</a></p>
Deep Learning for Microfluidic Assisted Caenorhabditis elegans Multi-parameter Identification Using YOLOv7
<p>The effectiveness of deep learning model relies on a large number of labeled image datasets. We have collected a dataset of 3373 worm images from microfluidic devices in various studies as datasets. Then, the datasets were labeled for training methods. The acquired videos were continuously intercepted at 10-frame intervals to capture cropped worm images. Totally 3931 images were extract. Worms in each image were manually annotated using LabelImg. This VOC-format annotation tool generated Extensible Markup Language (XML) files for the model. There were 2426 labels for the WT category and 1505 for the GFP category.</p>
Electric-field-induced anion-π catalysis on carbon nanotubes in electrochemical microfluidic devices: Original Data
<p>Original data underlying the publication entitled "Electric-field-induced anion-π catalysis on carbon nanotubes in electrochemical microfluidic devices"</p> <p>1 Synthesis: synthetic procedures (notebook pages) and data (NMR, IR, MS) of compounds <strong>6</strong>–<strong>8</strong>, <strong>15</strong>, <strong>17</strong>, <strong>19</strong>, <strong>21</strong>–<strong>26</strong></p> <p>2 Catalysis in suspension: procedure (notebook pages) and HPLC chromatograms (for compounds <strong>6</strong> and <strong>8</strong>) or NMR spectra (for compound<strong> 9</strong>)</p> <p>3 Catalysis using electromicrofluidics: procedure (notebook pages) and HPLC chromatograms</p>
Microfluidics Versus Gradient Centrifugation Effect on Euploidy Rates
ClinicalTrials.gov study NCT04744025. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.
Evaluating the Utility of Implementing Microfluids for Sperm Preparation Compared to Conventional Method of Density Gradient Centrifugation in a PGT-A Program: a Sibling Oocyte Study
ClinicalTrials.gov study NCT07093619. IPD Sharing: UNDECIDED. Countries: 1. Publications: 10.
Prognostic Value of Liver Cancer CTCs Isolated by a Novel Microfluidic Platform
ClinicalTrials.gov study NCT05242237. IPD Sharing: YES. Countries: 1. Publications: 9.
Microfluidics and Transcriptomics in Post Solid Organ Transplant Patients
ClinicalTrials.gov study NCT02776111. IPD Sharing: NO. Countries: 1. Publications: 45.
Using Microfluidic Separation Sperm Selection for Unexplained Infertility and Reccurrent Implantation Failure
ClinicalTrials.gov study NCT03355937. IPD Sharing: NO. Countries: 1. Publications: 1.
Integrated Microfluidic System for Oral Diagnostics
ClinicalTrials.gov study NCT00277745. IPD Sharing: Not stated. Countries: 1. Publications: 6.
Microfluidic Chip Method Versus Density-gradient Centrifugation Method in IVF
ClinicalTrials.gov study NCT06005311. IPD Sharing: YES. Countries: 1. Publications: 0.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
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DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.