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opencc-zeroOct 2021View details →

UCE and Sanger sequenced data for phylogenetic analysis of jumping spiders (Baviini and Nungia, Salticidae)

The systematics and taxonomy of the tropical Asian jumping spiders of the tribe Baviini is reviewed, with a molecular phylogenetic study (UCE sequence capture, traditional Sanger sequencing) guiding a reclassification of the group's genera. The well-studied members of the group are placed into six genera: Bavia Simon, 1877, Indopadilla Caleb & Sankaran, 2019, Padillothorax Simon, 1901, Piranthus Thorell, 1895, Stagetillus Simon, 1885, and one new genus, Maripanthus Maddison. The identity of Padillothorax is clarified, and Bavirecta Kanesharatnam & Benjamin, 2018 synonymized with it. Hyctiota Strand, 1911 is synonymized with Stagetillus. The molecular phylogeny divides the baviines into three clades, the Piranthus clade with a long embolus (Piranthus, Maripanthus), the genus Padillothorax with a flat body and short embolus, and the Bavia clade with a higher body and (usually) short embolus (remaining genera). In general, morphological synapomorphies support or extend the molecularly-delimited groups. Eighteen new species are described (all with taxonomic authority W. Maddison): Bavia nessagyna, Indopadilla bamilin, I. kodagura, I. nesinor, I. redunca, I. redynis, I. sabivia, I. vimedaba, Maripanthus draconis (type species of Maripanthus), M. jubatus, M. reinholdae, Padillothorax badut, P. mulu, Piranthus api, P. bakau, P. kohi, P. mandai, and Stagetillus irri. The distinctions between baviines and the astioid Nungia Żabka, 1985 are reviewed, leading to four species being moved into Nungia from Bavia and other genera. Fifteen new combinations are established, and one combination is restored. Five of these new or restored combinations correct previous errors of placing species in genera that have superficially similar palps but extremely different body forms, in fact belonging in distantly related tribes — emphasizing that the general shape of male palps should be used with caution in determining relationships. A little-studied genus, Padillothorus Prószyński, 2018, is tentatively assigned to the Baviini. Ligdus Thorell, 1895 is assigned to the Ballini.

opencc-by-4.0Jan 2018View details →

Sanger sequencing of PBMC derived B cells from human healthy donor

Sanger sequencing of 117 PBMC- derived B cells from human healthy donor

opencc-by-4.0Oct 2024View details →

The Sanger sequencing data of HCAPV-1

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opencc-by-4.0Dec 2018View details →

Figure 5. The polymorphism sites among the 25 in Biased heteroplasmy within the mitogenomic sequences of Gigantometra gigas revealed by sanger and high-throughput methods

Figure 5. The polymorphism sites among the 25 different cloning sequences of cox1. Weblogo 3.0 was used to show the nucleotide content of 25 cloning sequences of cox1 (Crooks et al., 2004). The abscissa stands for the number of the bases, while the ordinate stands for the proportion of nucleotide content provided by the 25 different cloning sequences in the same position. The sequence length between the two arrows stands for the barcode fragment size of cox1. The black triangles show the polymorphism positions in the 25 different cloning sequences, the red circles show the positions exhibited obvious second-peak in the results of direct Sanger sequencing without cloning, and the yellow stars show the different sites between the results of Sanger and HTS.

opencc-by-4.0Dec 2018View details →

Figure 2 in Biased heteroplasmy within the mitogenomic sequences of Gigantometra gigas revealed by sanger and high-throughput methods

Figure 2. The different nucleotides of all 13 PCGs in mitogenomes obtained by Sanger and HTS sequencing. The different sequences of HTS sequencing are separately compared with the consequence of Sanger method. The horizontal axis stands for the nucleotide position, of which the sequences of 13 PCGs are ordered according to the circular mitochondrial DNA from nad2 to nad1 in the clockwise direction. The vertical axis stands for the number of the different sites in the sequences of 13 PCGs, and the different nucleotide in each site of all three HTS sequences compared with Sanger is shown in the corresponding panel.

FIGURE 32 Occulta ocnus comb.n in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)

FIGURE 32 Occulta ocnus comb.n. Locality records.

FIGURE 29 Trico tricolor comb.n in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)

FIGURE 29 Trico tricolor comb.n. Locality records.

FIGURE 25 Xenovena murrayae comb.n in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)

FIGURE 25 Xenovena murrayae comb.n. Locality records.

FIGURE 22 Taguaiba gen.n in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)

FIGURE 22 Taguaiba gen.n. Species diversity mapped on a 2 degree grid.

openMay 2023View details →

Ceratodon purpureus APT-KO Sanger Sequencing

All Sanger sequencing data of APT-KOs in Ceratodon purpureus and knock-ins into the designated CpLand site.

publicDec 2015View details →

Data from: Validation of targeted next-generation sequencing for RAS mutation detection in FFPE colorectal cancer tissues: comparison with Sanger sequencing and ARMS-Scorpion real-time PCR

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publicJan 2019View details →

Data from: A sequel to Sanger: amplicon sequencing that scales

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publicOct 2021View details →

UCE and Sanger sequenced data for phylogenetic analysis of jumping spiders (Baviini and Nungia, Salticidae)

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ClinicalTrials.gov24/100

Sanger Human Cell Atlasing Project

ClinicalTrials.gov study NCT06497673. IPD Sharing: Not stated. Countries: 1. Publications: 0.

restrictedIPD-UNDECIDEDFeb 2026View details →

zenodo20/100

TABLE 1 in Combining target enrichment and Sanger sequencing data to clarify the systematics of the diverse Neotropical butterfly subtribe Euptychiina (Nymphalidae, Satyrinae)

TABLE 1 Comparison of characters for distinguishing Deltaya gen.n., Modica gen.n. and related genera.<table><tbody><tr><th></th><th>Modica gen.n.</th><th>Emeryus</th><th>Paryphthimoides</th><th>Colombeia</th><th>Scriptor</th><th>Deltaya gen.n.</th><th>Malaveria</th></tr></tbody><tbody><tr><th>Eyes: hair-like setae</th><td>Present</td><td>Absent</td><td>Present</td><td>Present</td><td>Present</td><td>Present</td><td>Present</td></tr><tr><th>DHW pale pupil dots in ocellus in cell Cu2-Cu1</th><td>Visible</td><td>Not visible</td><td>Variably visible</td><td>Variably visible</td><td>Not visible</td><td>Not visible</td><td>Not visible</td></tr><tr><th>VFW: prominent dark brown band (umbra) underlying postdiscal ocelli</th><td>Yes</td><td>No</td><td>Yes, somewhat (except almost absent in P. poltys, P. vestigiata)</td><td>Yes</td><td>Yes</td><td>Yes</td><td>No or weak umbra</td></tr><tr><th>VHW: dark marginal line in tornus</th><td>Thin, not broadening</td><td>Thin, not broadening</td><td>Thin, not broadening (except P. sheba, P. pseudoconfusa)</td><td>Marginal line slightly broader throughout wing</td><td>Broadening</td><td>Broadening</td><td>Thin, not broadening</td></tr><tr><th>VHW: postdiscal ocelli in cells Cu1- M3 and M3-M2</th><td>Double pupils (silver dots or elongate dashes) distinctly ringed with yellow</td><td>Where present, double pupils (silver dots) distinctly ringed with yellow</td><td>Variable across species, either double pupils (silver dots) distinctly ringed with yellow, or single pupil (an elongate silver smudge) indistinctly ringed with orange</td><td>Where present, double pupils (silver dots) distinctly ringed with yellow</td><td>Single pupil (an elongate silver smudge) indistinctly ringed with orange</td><td>Double pupils (silver dots or elongate dashes) distinctly ringed with yellow</td><td>Double pupils (silver dots or elongate dashes) distinctly ringed with yellow</td></tr><tr><th>Male genitalia aedeagus: cornuti</th><td>Variably present</td><td>Present</td><td>Present</td><td>Absent</td><td>Absent</td><td>Present (except in D. andrei and D. probata)</td><td>Absent</td></tr><tr><th>Female genitalia: lamella antevaginalis</th><td>No sclerotized lamella antevaginalis</td><td>Wrinkled, sclerotized lamella antevaginalis</td><td>Sclerotized lamella antevaginalis in some species</td><td>Sclerotized lamella antevaginalis</td><td>No sclerotized lamella antevaginalis</td><td>Sclerotized lamella antevaginalis (except in D. andrei and D. probata)</td><td>Sclerotized ‘spike’-like lamella antevaginalis</td></tr></tbody></table>