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FIGURE 1 in Crenidorsum aroidephagus Martin & Aguiar sp. nov. (Sternorrhyncha: Aleyrodidae), a New World whitefly species now colonising cultivated Araceae in Europe, Macaronesia and The Pacific Region
FIGURE 1. Computer-montage image of slidemounted puparium of Crenidorsum aroidephagus Martin & Aguiar sp. nov. ex-Philodendron gloriosum, Berlin Botanic Garden, with lingula unfolded and excluded from vasiform orifice.
FIGURE 5 in A new species of South American whitefly (Sternorrhyncha: Aleyrodidae) colonising cultivated bay laurel
FIGURE 5 (A-G). Aleuroplatus biluminiporus sp. nov. A. Habitus photograph of puparium. B. Scanning electron micrograph (SEM) of puparial vasiform orifice, with operculum raised. C. SEM of first-instar larva, habitus position. D. SEM of first-instar larva, thoracic marginal flanges. E. SEM of first-instar larva, abdominal marginal projections. F. SEM of first-instar larva, vasiform orifice. G. SEM of a group of eggs.
Fig. 9 in Comparing morphology of Myzus persicae regarding the taxonomic clarification of a subspecies colonising tobacco
Fig. 9. SEM images of sensilla on the hind tibia of Myzus persicae nicotianae (A-D) and Myzus persicae persicae (E-H). (A, E) The leg of L2 apterous larvae with ventrally located, short type-I trichoid sensilla and a dorsally located long, curved type-I trichoid sensilla. (B, F) Apterous adult leg with evenly distributed type-I trichoid sensilla of the same length. (C, G) Type-I trichoid sensilla are elongated on the dorsal side of the hind tibia of alate L3-L4 nymphs. (D, H) Alate adults bear the same short type-I trichoid sensilla like apterae ones.
Fig. 3 in Comparing morphology of Myzus persicae regarding the taxonomic clarification of a subspecies colonising tobacco
Fig. 3. SEM images of different stages of alate Myzus persicae nicotianae. (A–A′′′′) Larval stage two to three (L2-L3), (B–B′′′′) L3-L4 nymph bearing wing sheaths, and (C–C′′′′) alata. (A′-C′) Dorsal view of scabrous aphid head with distinctive vertex and diverging antennal tubercles (C′). Details of the thorax with enlarged second segment (A′′) and the base of wing buds (B′′), or fully evolved wings (C′′). The abdomen (A′′′ -C′′′) and the posterior abdominal tergites with siphunculi and cauda (A′′′′-C′′′′).
Fig. 1 in Comparing morphology of Myzus persicae regarding the taxonomic clarification of a subspecies colonising tobacco
Fig. 1. Schematic sketch of green peach aphid morphology. (A) Overview of a winged adult aphid (alata) displaying the different features analysed for morphological differentiation. [1] length of the hind femur (hf); [2] length of hind tibia (ht). (B) Aphid antenna segments (I-VI). [I] scape; [II] pedicel; [III- VI] flagellum; [at] antennal tubercle; [pt] processus terminalis; [1] length of antennal segment III (ant III); [2] diameter of the basal part of segment III (dante III); [3] length of trichoid sensilla on antennal segment III (sensant III), V (sensant V) or base of segment VI (sensant VI); [4] length of the base of segment VI (base VI); [5] length of processus terminalis (pt). (C) Ventral view of the head showing the rostrum. Distance between antennal tubercles (tb); the length of the trichoid sensilla located medially on antennal tubercles (hat). (D) Siphunculus with indicated morphometric characters. Length of siphunculus (ls); minimal width of the proximal part ('stem') of siphunculus (pws); maximal width of distal, swollen part of siphunculus (mws).
Fig. 6 in Comparing morphology of Myzus persicae regarding the taxonomic clarification of a subspecies colonising tobacco
Fig. 6. SEM images of the antenna and corresponding sensilla of the apterous L1 larva (A–A′′′), apterous L2 larva (B–B′′′), and apterous adult (C–C′′′), as well as alate L2-L3 nymph (D–D′′′), alate L3-L4 nymph (E–E′′′), and alate adult (F–F′′′) of Myzus persicae nicotianae. (A–F) The third antennal segment with (B–F) or without (A) type-I trichoid sensilla (blue-coloured). Adult alate specimens bore small multiporous placoid sensilla (secondary rhinaria, pink-coloured) within cuticle cavities along the segment (F). (A′-F′) The distal fifth segment with type-I trichoid sensilla (blue-coloured) and big multiporous placoid sensillum (secondary rhinarium, redcoloured), partially covered by cuticular protrusions. (A′′-F′′) The base of the antennal segment VI with multiple big placoid sensilla (primary rhinaria, red-coloured), type-I (green-coloured), and type-II (yellow-coloured) sunken coeloconic rhinaria, all enclosed by cuticle protrusions in separate cavities. (A′′′ -F′′′) Distal processus terminalis with four type-II trichoid sensilla (purple-coloured).
Fig. 5 in Comparing morphology of Myzus persicae regarding the taxonomic clarification of a subspecies colonising tobacco
Fig. 5. SEM images of juvenile and adult alate Myzus persicae persicae. Nymph at developmental stages two to three, L2-L3 (A–A′′′′) or three to four, L3-L4 (B–B′′′′), and adult (C–C′′′′). The head and antennal tubercles (A′, B′) with diverging features and distinctive vertex (C′). The thoracic segments show an enlarged, still wingless second segment (A′′), the roots of the nymphal wing buds (B′′), and the segment with the attachment point of the forewings (C′′). The overview of the abdominal tergites identifies structural differences between juveniles (A′′′, B′′′) and adult (C′′′). Posterior abdominal tergites and cauda with the siphunculi (A′′′′, B′′′′) exhibiting distal swelling (C′′′′).
Fig. 4 in Comparing morphology of Myzus persicae regarding the taxonomic clarification of a subspecies colonising tobacco
Fig. 4. SEM images of different morphs of apterous Myzus persicae persicae. Larva at developmental stage one, L1 (A–A′′′′) and two, L2 (B–B′′′′), and adult (C–C′′′′). The dorsal, wrinkled head (A′, B′) with some spicules (C′). The three dorsally sculptured thoracic segments (A′′-C′′) and the abdomen (A′′′-C′′′). (A′′′′ -C′′′′) Details of the posterior abdominal tergites with the dorsally located, compressed, and distally swollen (A′′′′, B′′′′) or long cylindrical (C′′′′) siphunculi and the transition to the cauda.
Fig. 2 in Comparing morphology of Myzus persicae regarding the taxonomic clarification of a subspecies colonising tobacco
Fig. 2. SEM images of different stages of apterous Myzus persicae nicotianae. (A–A′′′′) L1, (B–B′′′′) L2, (C–C′′′′) adult. (A′-C′) Dorsal head structure. (A′′-C′′) Thoracic segments. (A′′′-C′′′) Abdominal segments. (A′′′′ -C′′′′) The 8th tergite and the cauda with siphunculi.
Fig. 7 in Comparing morphology of Myzus persicae regarding the taxonomic clarification of a subspecies colonising tobacco
Fig. 7. SEM images of the antennal segments III, V, VI, and corresponding sensilla of Myzus persicae persicae. Apterous L1 (A–A′′′), L2 (B–B′′′), apterous adult (C–C′′ ′), alate L2-L3 nymph (D–D′′′), alate L3-4 nymph (E), and alate adult (F). Antennal segment III without sensilla (A, B) or with evenly distributed type-I trichoid sensilla (blue-coloured, C–F). Adult alate also bore small multiporous placoid sensilla (secondary rhinaria, pink-coloured, F). Segment V with type-I trichoid sensilla (blue-coloured,C′,D′) and big multiporous placoid sensillum (red-coloured) enclosed in a cuticular cavity and covered by protrusions (A′, B′, E′, F′). (A′′-F′′) Base of the sixth segment with type-I trichoid sensilla (blue-coloured) and a group of primary rhinaria composed of three big multiporous placoid sensilla of different sizes (red-coloured), and sunken type-I (green-coloured) and type-II (yellow-coloured) coeloconic sensilla. (A′′′-F′′′) Group of four type-II trichoid sensilla (purple-coloured) at the distal terminal process.
Fig. 8 in Comparing morphology of Myzus persicae regarding the taxonomic clarification of a subspecies colonising tobacco
Fig. 8. SEM images of wings of adult alatae Myzus persicae nicotianae and Myzus persicae persicae (A–D) and nymphal wing buds (E, F). Adult M. p. nicotianae forewing (A′) and hindwing (A′′) surface with distinct crescent- and arrow-shaped structures covering the edges. (B) The M. p. persicae fore- and hindwing surface. (B′, B′′) Small crescent-shaped structures cover the distal parts and veins of the wing with centered small knobby structures. (B′) An arrow marks a hamulus on the claval apparatus of the hindwing. (C) White arrows point to the hamuli on the hindwings of alate specimen of M. p. persicae. Hamuli on the left (C′) and right (C′′) hindwing are surrounded by numerous crescent-shaped structures that also cover the veins and distal parts of the wing. (D) The fore- and hindwing of an alate M. p. persicae show the pterostigma and surrounding structures on the wing membrane with scale-shaped elements of varying shapes (D′). Nymphal wing buds of M. p. persicae (E) and M. p. nicotianae (F).
Supplementary material 3 from: Machado A, Rodríguez-Expósito E, López M, Hernández M (2017) Phylogenetic analysis of the genus Laparocerus, with comments on colonisation and diversification in Macaronesia (Coleoptera, Curculionidae, Entiminae). ZooKeys 651: 1-77. https://doi.org/10.3897/zookeys.651.10097
Chronogram of genus Laparocerus Schönherr, 1834 from Macaronesia (Coleoptera, Curculionidae, Entiminae) : Explanation note: Timetree generated with MEGA7 (Kumar et al. 2016) using the RelTime method. Divergence times for all branching points in the Bayesian phylogram (COII, 16S rRNA, 12S rRNA) calculated using Maximum Likelihood method with the General Time Reversible model. Relative times were optimized and converted to absolute divergence times (shown next to branching points) based on not allowing species cluster marked with ♦ to be older than the age of the island of La Palma (1,72 Ma) or younger than 0.21 Ma. Bars around each node represent 95% confidence intervals which were computed using the method described in Tamura et al. (2013). The estimated log likelihood value is -32807.3327. A discrete gamma distribution was used to model evolutionary rate differences among sites (6 categories (+G, parameter = 0.5622)). The rate variation model allowed for some sites to be evolutionarily invariable ([+I], 42.7318% sites). The analysis involved 255 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 1357 positions in the final dataset. Taxon codes include an abbreviation of species name, its code number, and the initial of the island of origin (see Appendix 1).
Supplementary material 2 from: Machado A, Rodríguez-Expósito E, López M, Hernández M (2017) Phylogenetic analysis of the genus Laparocerus, with comments on colonisation and diversification in Macaronesia (Coleoptera, Curculionidae, Entiminae). ZooKeys 651: 1-77. https://doi.org/10.3897/zookeys.651.10097
4-gene phylogram of genus Laparocerus Schönherr, 1834 from Macaronesia (Coleoptera, Curculionidae, Entiminae) : Explanation note: Bayesian 50% majority rule consensus tree for COII, 16SrNA, 12S rRNA, and 28S rRNA: posterior probabilities above the branches. The analysis involved 245 nucleotide sequences. All positions containing gaps and missing data were eliminated except in the 12S rRNA sequence (gapcoded). There was a total of 2153 positions in the final dataset. Scale bar = genetic divergence.
Supplementary material 1 from: Machado A, Rodríguez-Expósito E, López M, Hernández M (2017) Phylogenetic analysis of the genus Laparocerus, with comments on colonisation and diversification in Macaronesia (Coleoptera, Curculionidae, Entiminae). ZooKeys 651: 1-77. https://doi.org/10.3897/zookeys.651.10097
Mitochondrial 3-gene phylogram of genus Laparocerus Schönherr, 1834 from Macaronesia (Coleoptera, Curculionidae, Entiminae) : Explanation note: Bayesian 50% majority rule consensus tree for COII, 16SrNA, and 12S rRNA: posterior probabilities above the branches. The analysis involved 256 nucleotide sequences. All positions containing gaps and missing data were eliminated except in the 12S rRNA sequence (gapcoded). There was a total of 1389 positions in the final dataset total OTUs = 256. Scale, genetic divergence.
Supplementary material 2 from: Gehrke B (2018) Staying cool: preadaptation to temperate climates required for colonising tropical alpine-like environments. PhytoKeys 96: 111-125. https://doi.org/10.3897/phytokeys.96.13353
List of genera investigated for the analysis including information on generic distribution, coding and references to the literature used :
FIGURES 3–6 in A new whitefly genus and species, Aleuroparvus theae Dubey (Hemiptera: Aleyrodidae) colonising Assam tea (Camellia sinensis) and Cinnamomum bejolghota, in North-East India
FIGURES 3–6. Aleuroparvus theae gen. et sp. nov., camera lucida drawings, 3, puparium, dorsal and ventral views; 4, thoracic tracheal pore and submarginal wax gland; 5, vasiform orifice and caudal furrow; 6, legs and antenna.
FIGURES 21–27 in A new whitefly genus and species, Aleuroparvus theae Dubey (Hemiptera: Aleyrodidae) colonising Assam tea (Camellia sinensis) and Cinnamomum bejolghota, in North-East India
FIGURES 21–27 Aleuroparvus theae gen. et sp. nov., scanning electron microscope images, puparium, 21, dorsal view; 22, abdominal area; 23, caudal furrow; 24, margin and submarginal bands; 25, thoracic tracheal pore opening; 26, submarginal wax gland; 27, eighth abdominal setae (broken), vasiform orifice and caudal furrow.
FIGURES 28–33 in A new whitefly genus and species, Aleuroparvus theae Dubey (Hemiptera: Aleyrodidae) colonising Assam tea (Camellia sinensis) and Cinnamomum bejolghota, in North-East India
FIGURES 28–33. Aleuroparvus theae gen. et sp. nov., scanning electron microscope images, puparium, 28, puparium, ventrolateral view showing obliquely downward extension of submargin; 29, ventral view; 30, thoracic tracheal fold; 31, legs, rostrum and adhesive sacs; 32, ventral setae and caudal tracheal fold; 33, antenna (RS– rostrum, AP– adhesive pads, ANT– antenna).
FIGURES 1–2 in A new whitefly genus and species, Aleuroparvus theae Dubey (Hemiptera: Aleyrodidae) colonising Assam tea (Camellia sinensis) and Cinnamomum bejolghota, in North-East India
FIGURES 1–2. Aleuroparvus theae gen. et sp. nov., habitus, 1, puparium on Cinnamomum bejolghota showing white wax secretion at thoracic tracheal pore area, and submarginal ridge; 2, puparium on tea leaf.
FIGURES 7–14 in A new whitefly genus and species, Aleuroparvus theae Dubey (Hemiptera: Aleyrodidae) colonising Assam tea (Camellia sinensis) and Cinnamomum bejolghota, in North-East India
FIGURES 7–14. Aleuroparvus theae gen. et sp. nov., photomicrographs, holotype puparium, 7, dorsal and ventral views; 8, margin, tracheal pore, submarginal wax secreting gland; 9, cephalothorax, partly separated submargin; 10, abdominal segment sutures, pockets and depressions; 11, submarginal wax glands and geminate pores; 12, vasiform orifice, caudal furrow and 8th abdominal setae; 13, cephalic seta; 14, first abdominal seta (FAS– first abdominal seta).
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