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FIGURE 5 in A Second Specimen of Citipati osmolskae Associated with a Nest of Eggs from Ukhaa Tolgod, Omnogov Aimag, Mongolia

FIGURE 5. Death Row sub locality of the Camels Humps. Arrow signifies where IGM 100/1004 was excavated.

FIGURE 4. IGM 100 in A Second Specimen of Citipati osmolskae Associated with a Nest of Eggs from Ukhaa Tolgod, Omnogov Aimag, Mongolia

FIGURE 4. IGM 100/1004. An adult Citipati osmolskae collected in 1995 from the Death Row sublocality at Ukhaa Tolgod, Omnogov Aimag, Mongolia, in dorsal view (opposite page and above).

FIGURE 1. AMNH FARB 6517 in A Second Specimen of Citipati osmolskae Associated with a Nest of Eggs from Ukhaa Tolgod, Omnogov Aimag, Mongolia

FIGURE 1. AMNH FARB 6517 the type specimen of Oviraptor philoceratops found associated with a nest of eggs. From Osborn (1924).

FIGURE 2. IGM 100 in A Second Specimen of Citipati osmolskae Associated with a Nest of Eggs from Ukhaa Tolgod, Omnogov Aimag, Mongolia

FIGURE 2. IGM 100/979. The nesting Citipati osmolskae as it was first found at Ukhaa Tolgod in 1993. Left Amy Davidson, right Louis Chiappe.

Operating diagram of hatching module, this module consists of two clearly separated sections, each consisting of two long tanks (2 × 0.2 × 0.2 m) designed to accommodate hatching boxes, a filtration tank and an independent water circulation pump with a cooling unit and UV sterilizer. This allows simultaneous monitoring of 16 batches of eggs. in Reproduction of Zingel asper (Linnaeus, 1758) in controlled conditions: an assessment of the experiences realized since 2005 at the Besançon Natural History Museum

Operating diagram of hatching module, this module consists of two clearly separated sections, each consisting of two long tanks (2 × 0.2 × 0.2 m) designed to accommodate hatching boxes, a filtration tank and an independent water circulation pump with a cooling unit and UV sterilizer. This allows simultaneous monitoring of 16 batches of eggs.

Operating diagram of hatching module in Zoug jars, this system consists of a 300-litre temperature-controlled isothermal enclosure containing 10 one-litre Zoug jars, each able to accommodate several hundred eggs. An ascending current holds the eggs in suspension and carries the larvae to the surface. Another bottle connected to this device collects the larvae. The water circulating in the jars is independent of that used in the filtration circuit. A cooling unit and UV sterilizer complete the installation. in Reproduction of Zingel asper (Linnaeus, 1758) in controlled conditions: an assessment of the experiences realized since 2005 at the Besançon Natural History Museum

Operating diagram of hatching module in Zoug jars, this system consists of a 300-litre temperature-controlled isothermal enclosure containing 10 one-litre Zoug jars, each able to accommodate several hundred eggs. An ascending current holds the eggs in suspension and carries the larvae to the surface. Another bottle connected to this device collects the larvae. The water circulating in the jars is independent of that used in the filtration circuit. A cooling unit and UV sterilizer complete the installation.

Operating diagram of IF incubator, two identical incubators were used for eggs that were already sorted. They are made up of an isothermal enclosure and contain three tiers (100 × 60 × 17 cm). The water circulating in each tier comes from the same filtration, cooling and sterilisation device. As a result, the eggs placed in the different tiers are subject to the same temperature regime. in Reproduction of Zingel asper (Linnaeus, 1758) in controlled conditions: an assessment of the experiences realized since 2005 at the Besançon Natural History Museum

Operating diagram of IF incubator, two identical incubators were used for eggs that were already sorted. They are made up of an isothermal enclosure and contain three tiers (100 × 60 × 17 cm). The water circulating in each tier comes from the same filtration, cooling and sterilisation device. As a result, the eggs placed in the different tiers are subject to the same temperature regime.

Operating diagram of the incubator, two tiered modules contain six independent incubators. Three shallow hatching are (220 × 60 × 17 cm) stacked on top of each other to create a compact assembly in which each tier functions independently. Eighteen trays covered with eggs can be placed in each tier, allowing the simultaneous incubation of seven to nine lays. in Reproduction of Zingel asper (Linnaeus, 1758) in controlled conditions: an assessment of the experiences realized since 2005 at the Besançon Natural History Museum

Operating diagram of the incubator, two tiered modules contain six independent incubators. Three shallow hatching are (220 × 60 × 17 cm) stacked on top of each other to create a compact assembly in which each tier functions independently. Eighteen trays covered with eggs can be placed in each tier, allowing the simultaneous incubation of seven to nine lays.

Figure 2. – Z. asper eggs. A in Reproduction of Zingel asper (Linnaeus, 1758) in controlled conditions: an assessment of the experiences realized since 2005 at the Besançon Natural History Museum

Figure 2. – Z. asper eggs. A: Mature ovum on the right and immature ovum on the left. B: Extraction of milt, this operation is done first. C: A low pressure from the abdomen towards the anus with the index finger allows the ova to be extracted. D: The yellow colour of the ova is a good indicator of their good quality. E: Water can only be added when everything is well mixed.

opencc-by-4.0Mar 2005View details →

Fig. 1 in The Influence Of Nest Size On Heat Loss Of Penduline Tit Eggs

Fig. 1. Changes in internal egg temperatures (mean °C) during trials with Penduline Tit nests (treatments: ambient temperature – clutch size)

opencc-by-4.0Mar 2005View details →

Fig. 2 in The Influence Of Nest Size On Heat Loss Of Penduline Tit Eggs

Fig. 2. Effects of nest thickness on terminal temperatures in relation to ambient temperature and clutch size. Each symbol represents one nest (N= 20 nests, all nests were measured in all treatments). We used least-squares regression to estimate the best fit to each pair of treatments separately (regression equations, 25°C & 9 eggs: terminal temperature = 27.64 + 0.05 × nest thickness; 25°C & 3 eggs: terminal temperature = 27.56 + 0.01 × nest thickness; 10°C & 9 eggs: terminal temperature = 16.29 + 0.19 × nest thickness; 10°C & 3 eggs: terminal temperature = 15.66 + 0.08 × nest thickness)

opencc-by-4.0Nov 2012View details →

Fig. 2 in Do Different Plasticine Eggs In Artificial Ground Nests Influence Nest Survival?

Fig. 2. Daily survival rates (+1SE) of different egg types in small mammal traps. Q: small mammal trap baited with one quail egg; NP: natural plasticine egg; WP: white coloured plasticine egg

opencc-by-4.0Nov 2012View details →

Fig. 1 in Do Different Plasticine Eggs In Artificial Ground Nests Influence Nest Survival?

Fig. 1. Daily survival rates (+1SE) of artificial ground nests with different egg combinations (Q+Q – nest baited with two quail eggs, Q+NP – one quail and one natural plasticine eggs, Q+WP – one quail and one white coloured plasticine eggs). White bars represent the survival rates, when damage or disappearance of any types of egg in the nest was considered as a predation event; gray bars represent the survival rates, when only damage or disappearance of quail eggs was considered as a predation event. Stars indicate significant differences, **: P <0.05; ***: P <0.01

opencc-by-4.0Jun 2017View details →

Figs 8–13. 8 – anterior spiracle. 9 in Micromorphology of egg and larva of Eristalis fratercula, with an updated key of Eristalis species with known third instar larvae (Diptera: Syrphidae)

Figs 8–13. 8 – anterior spiracle. 9 – two patches of sclerotized spicules. 10 – mesothoracic proleg; 11 – first abdominal proleg; 12 – sixth abdominal proleg; 13 – posterior breathing tube. Abbreviations: cs – central scars; fa – facets; ip – incurved plate; is – interspiracular setae; so – spiracular openings; sp – spiracular plate.

opencc-by-4.0Jun 2017View details →

Figs 2–7 in Micromorphology of egg and larva of Eristalis fratercula, with an updated key of Eristalis species with known third instar larvae (Diptera: Syrphidae)

Figs 2–7. Third instar larva of Eristalis fratercula (Zetterstedt, 1838). 2 – head and thoracic segments (pro- and mesothorax), ventral view; 3 – antennomaxillary organs; 4 – details of the cephalic region and lips; 5–6 – long branched spicules in the upper margin on the lateral lips; 7 – longitudinal grooves and anterior spiracles, dorsal view. Abbreviations: am – antennomaxillary organs; an – antenna; as – anterior spiracles; bs – branched spicules; dl – dorsal lips; es – extra pair of sensilla; ll – lateral lips; mp – maxillary palp; mtp – mesothoracic prolegs; ts – tuft of long setae; vl – ventral lips.

opencc-by-4.0Feb 2021View details →

Protocol for transfection by microinjection into the eggs of the parasite vector snail Biomphalaria glabrata

1. Egg production Place about 30 adult snails (10 mm diameter) into a 5.5-liter water tank. Place a piece of polystyrene of (3 x 3 cm) in each tank. There is the preferred support of Biomphalaria glabrata for laying its eggs. The snails are fed ad libitum with green lettuce leaves, they can also be fed with dry spirulina to boost reproduction. Maintain water at a temperature of 25 degrees Celsius. 2. Egg collection Gently pick up several egg layers from the polystyrene with soft holding forceps and place the eggs into a petri dish with natural mineral water (e.g. Volvic) to prevent them from drying out. Start sorting the eggs under the stereoscopic microscope to choose only the gastrula stage and place them into another petri dish with natural mineral water.   3. Preparation of the transfection solution Material: a. in vivo JetPEI transfection reagent b. 10% glucose solution c. 5% glucose solution d. Plasmids (dCas9-SunTag-BFP and scFv-DNMT3A-GFP) e. 0.2 ml microtubes f. P10 and P200 pipettes g. P10 and P200 pipette tips h. Permanent marker The glucose solution and the in vivo jetPEI transfection reagent are equilibrated at room temperature.  Prepare 21 µl of each plasmid at a concentration of 78 and 88 ng / µl respectively (for a total volume of 42 µl =equals 3.5 µg of DNA) add the plasmid DNA to a 0.2 ml tube (labeled as Tube A) and mix with 21 µl of 10% glucose solution.  In another microtube (labeled as Tube B), add 21 μl of 5% glucose solution and 1 μl of in vivo jetPEI.   Prepare a third tube (labeled as Tube C) with 21 µl of 5% glucose solution and 0.5 µl of in vivo jetPEI to inject into embryos that will serve as controls.  Leave the solutions at room temperature while you prepare the microinjection station.      4. Preparation of the micro-injection station Material: a. Pre-pulled glass micropipettes (1mm diameter) b. Watch glass c. Modeling clay d. 35 mm and 90 mm petri dishes e. Mineral oil (M5904, SIGMA) f. Wash bottle with natural mineral water (Volvic) g. 0.2 ml microtubes h. 12-well cell culture plate i. Fine brush j. Phenol red solution k. Pasteur pipette or dropper l. Dissection forceps m. Soft holding forceps n. Snail eggs in the gastrula stage o. Drummond Scientific Nanoject III Programmable Nanoliter Injector   Take a pre-pulled glass micropipette and cut it with a scalpel to have a ~ 0.2 mm tip slightly beveled if possible. Before attaching the micropipette to the programmable nanoliter injector, fill it with mineral oil. If this step is not done, the injector will not work properly. This can be done with a filling needle  attached to a hamilton syringe of 10 microliters. When the micropipette is filled with oil, it must be fixed on the injector. For this it is necessary to:  Slide the chuck and collet onto the glass micropipette, then slide the black O-ring with the seal onto the wire plunger  With the micropipette attached to the injector, press the [EMPTY] icon until the plunger is fully extended. This step can be done with the footswitch by pressing once [EMPTY] then [STOP] and then proceeding [EMPTY] with the foot switch. A single beep is emitted when the plunger is fully extended. Fill the micropipette with 3 µl of the control solution or the transfection solution by placing the glass micropipette tip in a 0.2 ml tube with the solution to be injected and pressing the [FILL] icon. It is desirable to fill it at a slow rate, by pressing the [FILL] icon for a few seconds, then the [STOP] icon to allow the sample to equilibrate before pressing again the '[FILL] icon. Note: The piston continues to extend or retract until the [STOP] icon is pressed, or until the fully extended or fully retracted position is reached.   5. Microinjection Place a watch glass into a 35mm petri dish and secure it on one side with modeling clay to form a slope. Use soft handling forceps to transfer an egg mass and lay it on the slope side of the watch glass so that the egg mass is in a sloping position. Remove excess water from the eggs with absorbent paper. Rehydrate if necessary with a fine brush to improve the visibility of the embryos. To inject the sample, return to the operating mode screen by pressing the [EXIT] icon, then select the injection mode by pressing the [INJECT] icon. Set the injection volume to 30nL and the flow rate to 20nL per second using the icons [+] and [-] respectively. Press the [INJECT] icon to inject the sample.  Inject 30nL of the microinjection solution into each egg. Place the microinjected egg masses in a 12-well cell culture plate and note with a marker whether they were microinjected with the control solution or with the solution containing the plasmids. We colored the injection solution with red phenol to facilitate the visibility in this video. Monitor the expression of the plasmids Monitor the plasmids expression 72 h after microinjection in a contrast / fluorescent microscope or in a fluorescent stereo microscope. Then sort the fluorescent snails and perform a second micro-injection with a solution containing 10 µl of single guide RNA (at a concentration of 2ng / µl), add 0.5 µl of in vivo jetPEI reagent and 10 µl of 5% glucose solution. 3 days after the second microinjection, collect the hatched snails in a 1.5 ml tube containing 25 µl of lysis buffer for DNA and RNA purification. In this photo produced under a confocal microscope we washed a veliger larva in PBS solution, then we fixed it with 4% paraformaldehyde solution and then we placed it in a slide with two drops of the Dako fluorescence mounting medium.  96 after the transfection we can observe the expression of the green fluorescent protein, the blue fluorescent protein and the co-localization of both proteins.  This protocol is used to perform DNA methylation changes in a target gene. This transfection protocol can be used with other plasmids, with small interfering RNAs, or with messenger RNAs. Produced at IHPE (http://ihpe.univ-perp.fr)

Fig. 2 in Pachyrhynchus obumanuvu sp. nov., a new species of easter egg weevil (Coleoptera, Curculionidae, Entiminae, Pachyrhynchini) from Mindanao Island, Philippines

Fig. 2. Male genitalia of Pachyrhynchus obumanuvu sp. nov.: A. penis in lateral view, B. idem. in dorsal view, C. sternite IX in dorsal view

Fig. 1 in Pachyrhynchus obumanuvu sp. nov., a new species of easter egg weevil (Coleoptera, Curculionidae, Entiminae, Pachyrhynchini) from Mindanao Island, Philippines

Fig. 1. Pachyrhynchus obumanuvu sp. nov. - A, C, Holotype male; A. dorsal view, C. lateral view. B, D, Paratype female; B. dorsal view, D. lateral view

Fig. 3 in Pachyrhynchus obumanuvu sp. nov., a new species of easter egg weevil (Coleoptera, Curculionidae, Entiminae, Pachyrhynchini) from Mindanao Island, Philippines

Fig. 3. Ecological notes about Pachyrhynchus obumanuvu sp. nov.: A. Habitat in Davao City, B. Procris urdanetensis Elmer, C. Elatostema sp., possible food plant, D. bitemarks on Elatostema sp., E. P. obumanuvu sp. nov perching on the leaves, F. P. obumanuvu sp. nov. on stem

Dataset for "Molecular modeling of the interface of an egg yolk protein-based emulsion"

Dataset for figures of upcoming article "Molecular modeling of the interface of an egg yolk protein-based emulsion" submitted to the journal "Physics of Fluids". dimerApovitellenin1_AA.pdb is the all-atom protein structure file used in MD simulations. DPDparameters.csv is the parametrization file used in DPD simulations.