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Data for Aerosol Diffuse Fertilization Effect
<p>The simulated GPP and diffuse PAR caused by natural and anthropogenic aerosols under all and clear skies.</p>
A time series database of available P concentrations and grass growth in soils receiving DPW fertilizers
<p>This data set contains a time-series database of available and exchangeable P concentrations and grass dry matter yield in soils receiving struvites and hydrochar produced from DPS</p>
Soil biological, chemical and physical parameters and herbage yield in a field experiment with organic and inorganic fertilizers on peat grassland in the Netherlands
<p>To evaluate the performance of organic and inorganic fertilizers for regeneration of ecosystem services in peat grasslands with biodiversity goals, we carried out a field experiment in the western peat district in the Netherlands. The fertilizers tested represent the current practice and potential alternatives for regenerative grassland management on drained peat.</p> <p> </p> <p><strong>Experimental setup</strong></p> <p>The field experiment (2013 – 2015) was conducted on a permanent grassland on peat soil (Terric Histosol; SOM 56 g 100 g<sup>−1</sup> and pH<sub>KCl</sub> of 4.5 in 0-10 cm) at the experimental dairy farm at Zegveld (the Netherlands). In March 2013, a randomized block experiment (six blocks) was laid out with six fertilizer treatments and a control treatment (no fertilizer: “Contr”). The fertilizer used were: conventional dairy cattle slurry manure (“Slurry”), mature compost of kitchen and garden waste (“Comp”), dairy cattle farmyard manure (“FYM”), solid fraction of the cattle slurry manure (“SFrac”, obtained by pressurized filtration), inorganic N fertilizer (“IF”; calcium ammonium nitrate, 27% N) and a combination of inorganic N fertilizer and sawdust (“IF+SD”). Plot size was 4 × 10 m; for the Slurry treatment plots were 5.2 × 10 m. Slurry was applied by slit injection, the other fertilizers were applied by hand. Target application rate was 120 kg total N ha<sup>−1</sup> yr<sup>−1</sup>, divided in two applications per year (February/March and May). This is relatively low for conventional grasslands but usual for grasslands with biodiversity goals (Kleijn et al., 2004). The amount of C<sub>total</sub> applied in Comp was taken for the rate of sawdust to be applied. All plots were fertilized with 200 kg K<sub>2</sub>O ha<sup>−1</sup> yr<sup>−1</sup> (applications in March and May) (Commissie Bemesting Grasland en Voedergewassen, 2019). Fertilizer application quantities and organic matter and nutrient inputs are provided in Fertilizer_intput.csv (dataset).</p> <p>The grassland had an history of conventional management with mainly cutting, winter grazing with sheep and a normal fertilization regime with both slurry manure and inorganic fertilizer. The normal cutting and grazing regime was continued in the first two years of the experiment; during 2015, the monitoring year, the plots were not grazed and only cut for herbage measurements.</p> <p> </p> <p><strong>Measurements</strong></p> <p>From April to October 2015, soil and aboveground measurements were carried out. Most soil parameters were measured in October. Earthworms and insect larvae are an important food source for meadow birds during the pre-breeding period in spring (Galbraith, 1989) and were therefore sampled in April. Soil moisture and penetration resistance were measured both in April and October.</p> <p> </p> <p><em>Soil biological parameters</em></p> <p>Earthworms and insect larvae were sampled in the top soil layer in two soil cubes (20 × 20 × 20 cm) per plot. Earthworms were hand-sorted, counted, weighed and fixed in alcohol prior to identification. Both adults and juveniles were identified to species (Sims and Gerard, 1985; Stöp-Bowitz, 1969) and classified into functional groups (Bouché, 1977). Crane flies (Tipulidae; leatherjackets) or click beetles (Elateridae; wireworms) larvae were counted.</p> <p>Phospholipid fatty acids (PLFA) were measured in October. PLFA were extracted from 4 g of fresh soil (Palojärvi, 2006), and analyzed by gas chromatography (Hewlett-Packard, USA). PLFA i15:0, a15:0, 15:0, i16:0, 16:1ω9, i17:0, a17:0, cy17:0, 18:1ω7 and cy19:0 were chosen to represent bacteria and PLFA 18:2ω6 was used as a marker of saprotrophic fungi (Hedlund, 2002). The neutral lipid fatty acid (NLFA) 16:1ω5 occurs in storage lipids of arbuscular mycorrhizal fungi (AMF) and was used as marker of AMF (Vestberg et al., 2012). PLFA i15:0, a15:0, i16:0, i17:0 and a17:0 were used as a measure of Gram-positive bacteria, and cy17:0 and cy19:0 for Gram-negative bacteria. PLFA 10Me16:0, 10Me17:0 and 10Me18:0 represented actinomycetes.</p> <p> </p> <p><em>Soil chemical parameters</em></p> <p>A soil sample from the 0−10 cm layer (c. 50 randomly taken soil cores) per experimental plot was collected in October (auger diameter 2.3 cm; Eijkelkamp grass plot sampler, Giesbeek, the Netherlands), was sieved (1 cm mesh size) and homogenized. One sub-sample was taken for analysis of hot water extractable carbon (HWC) according to Ghani et al. (2003) and one for chemical analysis. Prior to analysis of soil acidity (pH<sub>KCl</sub>), soil organic matter (SOM), total carbon (C<sub>total</sub>), total nitrogen (N<sub>total</sub>), total phosphorus (P<sub>total</sub>) and ammonium-lactate extractable P (P<sub>AL</sub>) by Eurofins Agro (Wageningen, the Netherlands), the sub sample was dried at 40°C. Soil pH<sub>KCl</sub> was measured according to NEN-ISO 10390 2005. SOM was determined by loss-on-ignition (NEN 5754 2005). C<sub>total</sub> was measured by incineration at 1150°C, and determination of the CO<sub>2</sub> produced by an infrared detector (LECO Corporation, St. Joseph, Mich., USA). For N<sub>total</sub>, evolved gasses after incineration were reduced to N<sub>2</sub> and measured with a thermal-conductivity detector (LECO Corporation, St. Joseph, Mich., USA). P<sub>total</sub> was analysed with Fleishmann acid (Houba et al., 1997). P<sub>AL</sub> is used to assess the P supply capacity of grassland soils (Reijneveld et al., 2014) and was determined according to Egnér et al. (1960) (NEN 5793).</p> <p> </p> <p><em>Soil physical parameters</em></p> <p>Soil moisture was determined in April and October in a homogenized 0−10 cm soil sample after drying at 105°C for 24 hrs. Moisture content was expressed as percentage of fresh soil weight.</p> <p>Penetration resistance was measured (April and October) with a penetrologger (Eijkelkamp, Giesbeek, the Netherlands; cone of 2.0 cm<sup>2</sup> penetration surface and 60° apex angle. Penetration resistance was expressed as an average of 7 penetrations per plot and per soil layer of 0−10, 10−20, and 20−30 cm.</p> <p>Soil structure and rooting density were assessed in October in the 0−10 cm and 10−25 cm layers. The percentage of crumbs, sub-angular blocky elements and angular blocky elements was estimated by one experienced person as described by Peerlkamp (1959) and Shepherd (2000), Root density was estimated by scoring visible roots (score 1–10; 1 for no roots and 10 for above average).</p> <p>Water infiltration rate was measured in October at three spots per experimental plot in 5 of the 6 blocks (35 plots). A PVC pipe (15 cm high, 15 cm diameter) was pushed into the soil to a depth of 10 cm. 500 ml water was poured into each pipe and the infiltration time was recorded. If the infiltration time exceeded 15 min, the remaining water volume was estimated to calculate the infiltration rate (mm min<sup>−1</sup>).</p> <p> </p> <p><em>Grass yield and botanical composition</em></p> <p>Grass dry matter (DM) and N yield were determined during 2015 with a Haldrup plot harvester (J. Haldrup a/s, Løgstør, Denmark). The four harvest dates were May 15, June 29, August 19 and September 30. Fresh biomass, DM content (70°C for 24 hrs) and total N content (Kjeldahl) were determined for each harvest. Herbage DM yield (Mg DM ha<sup>−1</sup>) and herbage N yield (kg N ha<sup>−1</sup>) were calculated. Apparent N recovery (ANR; kg N.kg N<sup>−1</sup>) was calculated as (N yield<sub>(fertilized)</sub> – N yield<sub>(non-fertilized)</sub>)/(N fertilization rate) (Vellinga and André, 1999).</p> <p>In June 2015, botanical composition was measured by visually estimating the relative soil cover of the sward and the proportion of each species therein (Sikkema, 1997).</p> <p> </p> <p><strong>Data files</strong></p> <ul> </ul> <p> </p> <p><em><strong>Data_soil_grass.csv</strong></em></p> <p><em>Content:</em></p> <p>Dataset with soil biological (earthworms, microbial PLFA), soil chemical, soil physical parameters, herbage dry matter and N yields, and botanical parameters.</p> <p><em>Column names and units:</em></p> <ul> <li>plot: Experimental plot number (1-42)</li> <li>treatment: Treatment code (see text)</li> <li>block: Block number (1-6)</li> <li>EW_species_number: Earthworm - number of species</li> <li>EW_totalnumber: Earthworm - total number per m2</li> <li>EW_epigeic: Earthworm - number of epigeic adults and juveniles per m2</li> <li>EW_endogeic: Earthworm - number of endogeic adults and juveniles per m2</li> <li>EW_adults: Earthworm - number of adults per m2</li> <li>EW_juveniles: Earthworm - number of juveniles per m2</li> <li>EW_adult_epigeic: Earthworm - number of epigeic adults per m2</li> <li>EW_adult_endogeic: Earthworm - number of endogeic adults per m2</li> <li>EW_juven_epigeic: Earthworm - number of epigeic juveniles per m2</li> <li>EW_juven_endogeic: Earthworm - number of endogeic juveniles per m2</li> <li>EW_L_rubellus: Earthworm - number of L. rubellus adults and juveniles per m2</li> <li>EW_A_chlorotica: Earthworm - number of A. chlorotica adults and juveniles per m2</li> <li>EW_A_caliginosa: Earthworm - number of A. caliginosa adults and juveniles per m2</li> <li>EW_O_lacteum: Earthworm - number of O. lacteum adults and juveniles per m2</li> <li>EW_A_rosea: Earthworm - number of A. rosea adults and juveniles per m2</li> <li>EW_O_cyaenum: Earthworm - number of O. cyaneum adults and juveniles per m2</li> <li>EW_L_castaneus: Earthworm - number of L. castaneus adults and juveniles per m2</li> <li>EW_D_rubida: Earthworm - number of D. rubida adults and juveniles per m2</li> <li>EW_adult_L_rubellus: Earthworm - number of L. rubellus adults per m2</li> <li>EW_adult_A_chlorotica: Earthworm - number of A. chlorotica adults per m2</li> <li>EW_adult_A_caliginosa: Earthworm - number of A. caliginosa adults per m2</li> <li>EW_adult_O_lacteum: Earthworm - number of O. lacteum adults per m2</li> <li>EW_adult_A_rosea: Earthworm - number of A. rosea adults per m2</li> <li>EW_adult_O_cyaenum: Earthworm - number of O. cyaneum adults per m2</li> <li>EW_adult_L_castaneus: Earthworm - number of L. castaneus adults per m2</li> <li>EW_adult_D_rubida: Earthworm - number of D. rubida adults per m2</li> <li>EW_juven_L_rubellus: Earthworm - number of L. rubellus juveniles per m2</li> <li>EW_juven_A_chlorotica: Earthworm - number of A. chlorotica juveniles per m2</li> <li>EW_juven_A_caliginosa: Earthworm - number of A. caliginosa juveniles per m2</li> <li>EW_non_determined: Earthworm - number of non determined individuals per m2</li> <li>EW_total_biomass: Earthworm - total fresh biomass per m2</li> <li>Leatherjackets: number of leatherjackets per m2</li> <li>Wireworms: number of wireworms per m2</li> <li>TOTmicrPLFA: total microbial PLFA in nmol.g-1 dry soil</li> <li>bactPLFA: bacterial PLFA in nmol.g-1 dry soil</li> <li>saprofungPLFA: saprotrophic fungal PLFA in nmol.g-1 dry soil</li> <li>Fung_bactPLAF_ratio: ratio of fungal to bacterial PLFA</li> <li>GramPLUSplfa: gram positive PLFA in nmol.g-1 dry soil</li> <li>GramMINplfa: gram negative PLFA in nmol.g-1 dry soil</li> <li>ratioGram_PLUS_MIN: ratio of gram positive to gram negative PLFA</li> <li>AMFsporNLFA: AMF spores NLFA in nmol.g-1 dry soil</li> <li>ActinomPLFA: Actinomycetes PLFA in nmol.g-1 dry soil</li> <li>ShannonPLFA: PLFA shannon diversity index</li> <li>SOM: soil organic matter in g.100 g-1 dry soil</li> <li>Ctotal: total C in g.100 g-1 dry soil</li> <li>HWC: hot water extractable C in μg.100 g-1 dry soil</li> <li>Ntotal: total N in g.100 g-1 dry soil</li> <li>Ptotal: total P2O5 in mg.100 g-1 dry soil</li> <li>P_AL: total P-AL in mg.100 g-1 dry soil</li> <li>pH_KCl: pH-KCl</li> <li>CN_ratio: C:N ratio</li> <li>C_SOM: C:SOM ratio</li> <li>Soilmoisture_April: soil moisture content in April in g.100g-1 fresh soil</li> <li>Penetrationresistance_April_cm010: penetration resistance in April in 10-20 cm in Newton</li> <li>Penetrationresistance_April_cm1020: penetration resistance in April in 20-30 cm in Newton</li> <li>Penetrationresistance_April_cm2030: penetration resistance in April in 0-10 cm in Newton</li> <li>Soilmoisture_October: soil moisture content in October in g.100g-1 fresh soil</li> <li>Penetrationresistance_October_cm010: penetration resistance in October in 10-20 cm in Newton</li> <li>Penetrationresistance_October_cm1020: penetration resistance in October in 20-30 cm in Newton</li> <li>Penetrationresistance_October_cm2030: penetration resistance in October in 0-10 cm in Newton</li> <li>crumb_struct_cm010: percentage of crumb elements in 0-10 cm</li> <li>round_struct_cm011: percentage of sub-angular elements in 0-10 cm</li> <li>rootdensity_cm010: score (1-10) of root density in 0-10 cm</li> <li>crumb_struct_cm1025: percentage of crumb elements in 10-25 cm</li> <li>round_struct_cm1025: percentage of sub-angular elements in 10-25 cm</li> <li>sharp_struct_cm1025: percentage of angular elements in 10-25 cm</li> <li>rootdensity_cm1025: score (1-10) of root density in 10-25 cm</li> <li>water_infiltration: water infiltration rate in mm per minute</li> <li>DM_yield_year: total herbage dry matter yield in kg.ha-1 per year</li> <li>DM_yield_H1: herbage dry matter yield of harvest 1 in kg.ha-1</li> <li>DM_yield_H2: herbage dry matter yield of harvest 2 in kg.ha-1</li> <li>DM_yield_H3: herbage dry matter yield of harvest 3 in kg.ha-1</li> <li>DM_yield_H4: herbage dry matter yield of harvest 4 in kg.ha-1</li> <li>N_yield_year: total herbage N yield in kg.ha-1 per year</li> <li>N_yield_H1: herbage N yield of harvest 1 in kg.ha-1</li> <li>N_yield_H2: herbage N yield of harvest 2 in kg.ha-1</li> <li>N_yield_H3: herbage N yield of harvest 3 in kg.ha-1</li> <li>N_yield_H4: herbage N yield of harvest 4 in kg.ha-1</li> <li>DMperc_yield_year: herbage dry matter content (per year; weighed average over the 4 harvests) in g.100g-1 fresh weight</li> <li>DMperc_yield_H1: herbage dry matter content of harvest 1 in g.100g-1 fresh weight</li> <li>DMperc_yield_H2: herbage dry matter content of harvest 2 in g.100g-1 fresh weight</li> <li>DMperc_yield_H3: herbage dry matter content of harvest 3 in g.100g-1 fresh weight</li> <li>DMperc_yield_H4: herbage dry matter content of harvest 4 in g.100g-1 fresh weight</li> <li>Ncontent_yield_year: herbage N content (per year; weighed average over the 4 harvests) in g.kg-1 dry matter</li> <li>Ncontent_yield_H1: herbage N content of harvest 1 in g.kg-1 dry matter</li> <li>Ncontent_yield_H2: herbage N content of harvest 2 in g.kg-1 dry matter</li> <li>Ncontent_yield_H3: herbage N content of harvest 3 in g.kg-1 dry matter</li> <li>Ncontent_yield_H4: herbage N content of harvest 4 in g.kg-1 dry matter</li> <li>fresh_yield_H1: herbvage fresh yield of harvest 1 in Mg.ha-1</li> <li>ANR: apparent N recovery in kg N.kg N-1</li> <li>productive_grasses: cover percentage of L. perenne and P trivialis</li> <li>monocotyledons: cover percentage of monocotyledons</li> <li>dicotyledons: cover percentage of dicotyledons</li> <li>plant_species: number of plant species</li> <li>monocot_species: number of monocotyledon species</li> <li>dicot_species: number of dicotyledon species</li> <li>Lolium_perenne: plant cover %</li> <li>Poa_trivialis: plant cover %</li> <li>Phleum_pratense: plant cover %</li> <li>Elytrigia_repens: plant cover %</li> <li>Poa_annua: plant cover %</li> <li>Agrostis_stolonifera: plant cover %</li> <li>Holcus_lanatus: plant cover %</li> <li>Alopecurus_pratensis: plant cover %</li> <li>Alopecurus_geniculatus: plant cover %</li> <li>Trifolium_repens: plant cover %</li> <li>Taraxacum_officinale: plant cover %</li> <li>Ranunculus_arvensis: plant cover %</li> <li>Rumex_obtusifolius: plant cover %</li> <li>Rumex_crispus: plant cover %</li> <li>Ranunculus_acris: plant cover %</li> <li>Stellaria_media: plant cover %</li> <li>Cardamine_pratensis: plant cover %</li> <li>Bellis_perennis: plant cover %</li> <li>Rumex_acetosa: plant cover %</li> <li>Ranunculus_sceleratus: plant cover %</li> <li>Polygonum_aviculare: plant cover %</li> <li>Capsella_bursa-pastoris: plant cover %</li> <li>Glechoma_hederacea: plant cover %</li> <li>Geranium_molle: plant cover %</li> </ul> <p> </p> <p><em><strong>Fertilizer_input.csv</strong></em></p> <p><em>Content:</em></p> <p>Application quantities of fertilizers and ash, organic matter, C and mineral inputs, and fertilizer C:N ratio. Total N input is the sum of mineral N (Nmin) and organic N (Norg). Average values per hectare and per year over the years 2013−2015.</p> <p><em>Column names and units:</em></p> <ul> <li>Treatment: Treatment code (see text)</li> <li>Fertilizer_fresh: Applied fertilizer in Mg.ha<sup>-1</sup> per year (fresh weight)</li> <li>Fertilizer_DM: Applied fertilizer in Mg.ha<sup>-1</sup> per year (dry matter weight); for IF+SD this is the sum of 2.72 Mg sawdust + 0.45 Mg N fertilizer</li> <li>Ash: Mineral fraction in kg.ha<sup>-1</sup> per year</li> <li>OM: Organic matter in kg.ha<sup>-1</sup> per year</li> <li>C: Total C in kg.ha<sup>-1</sup> per year</li> <li>Nmin: Mineral N in kg.ha<sup>-1</sup> per year</li> <li>Norg: Organic N in kg.ha<sup>-1</sup> per year</li> <li>P2O5: kg.ha<sup>-1</sup> per year</li> <li>C_N_ratio: C:N ratio</li> </ul>
D6.1- Fertilizers-PCFs-Contaminants-Regulation (EU) 2019/1009 _some EU Member states regulations
<p>The annex of deliverable D6.1 is a summary of the fertiliser contamination limits in each of the countries participating in the WalNUT project according to the national legislation of each country.</p>
Soil extracellular enzyme activities in plots dominated by trees that associate with arbuscular mycorrhizal or ectomycorrhizal fungi in the N fertilized and reference watershed at the Fernow Experimental Forest, WV.
Our objective was to detect possible differences in N fertilization responses of soil extracellular enzymes in plots dominated by trees that associate with arbuscular mycorrhizal fungi (AM) or ectomycorrhizal fungi (ECM). To do this, we established a plot network of 6 AM and 6 ECM dominated 10 x 10 m plots in both the reference and N fertilized watersheds (N=24 plots) at the Fernow Experimental Forest, Parsons, WV. We assayed the potential activity of hydrolytic enzymes that release N (N-acetylglucosaminidase; NAG), phosphorus (acid phosphatase; AP), and simple carbon (ß-glucosidase; BG). In addition, we measured microbial allocation to complex C degrading oxidative enzymes phenol oxidase and peroxidase. The activities of these enzymes were measured separately in bulk mineral, rhizosphere, and organic horizon soils during the growing season in 2017.
Midwest U.S. Fertilizer Data, 1985-2015
Sulfur, as an essential nutrient for plant growth, has increasingly been used in fertilizer applications for many crops. This increase is coincident with declines in atmospheric sulfur deposition in response to air quality improvements in the United States and Europe. Here, we provide data for sulfur fertilizer sales by mass, as a proxy for fertilizer application, and provide data to estimate total atmospheric sulfur deposition across the Midwestern United States, a 12-state region that includes: Iowa, Illinois, Indiana, Kansas, Michigan, Minnesota, Missouri, Nebraska, North Dakota, Ohio, South Dakota, and Wisconsin. In addition, we provide data on other major nutrient fertilizers, including nitrogen, phosphorus and potassium, to compare with sulfur. Fertilizer data are from the American Association for Plant Food Control Officials (AAPFCO) and are aggregated from the county-level to the 12-state Midwestern region for the period of 1985-2015.
Genome size influences plant growth and biodiversity responses to nutrient fertilization in diverse grassland communities
Experiments comparing diploids with polyploids and in single grassland sites show that nitrogen and/or phosphorus availability influences plant growth and community composition dependent on genome size; specifically plants with larger genomes grow faster under nutrient enrichments relative to those with smaller genomes. However, it is unknown if these effects are specific to particular site localities with speciifc plant assemblages, climates, and historical contingencies. To determine the generality of genome size dependent growth responses to nitrogen and phosphorus fertilisation, we combined genome size and species abundance data from 27 coordinated grassland nutrient addition experiments in the Nutrient Network that occur in the Northern Hemisphere across a range of climates and grassland communities. We found that after nitrogen treatment, species with larger genomes generally increased more in cover compared to those with smaller genomes, potentially due to a release from nutrient limitation. Responses were strongest for C3 grasses and in less seasonal, low precipitation environments, indicating that genome size effects on water-use-efficiency modulates genome size-nutrient interactions. Cumulatively the data suggest that genome size is informative and improves predictions of species’ success in grassland communities.
Extracellular enzyme activities in plots dominated by trees that associate with arbuscular mycorrhizal or ectomycorrhizal fungi in the nitrogen fertilized and reference watershed at the Bear Brook Watershed in Maine, USA during the final year of N fertilization (2016) and during the year after N fertilization ceased (2017).
Our objective was to detect possible differences in N fertilization responses of extracellular enzymes in plots dominated by trees that associate with arbuscular mycorrhizal fungi (AM) or ectomycorrhizal fungi (ECM). To do this, we established a plot network of 6 AM and 6 ECM dominated (>65% diameter at breast height) 10 x 10 m plots in the lower elevation hardwood zone of both the reference and N fertilized watersheds (N=24 plots) at Bear Brook Watershed, in Maine USA. We assayed the potential activity of hydrolytic enzymes that release N (N-acetylglucosaminidase; NAG), phosphorus (acid phosphatase; AP), and simple carbon (ß-glucosidase; BG). The activities of these enzymes were measured separately in bulk mineral, rhizosphere, and organic horizon soils in the final year of N fertilization at Bear Brook in 2016 and during the year after N fertilization ceased in 2017.
Abundance and biomass of major taxonomic groups of arthropods collected with pitfall and vacuum sampling in Arctic LTER plots fertilized for 24 years near Toolik Field Station, Alaska in the summer of 2013.
Arthropods (spiders and insects) were collected three times during the 2013 summer using pitfall traps and vacuum sampling in plots fertilized with Nitrogen and Phosphorus for 24 years, and in control plots, in an experiment established near Toolik Field Station, Alaska. Pitfall traps were placed for 48-hour intervals; vacuum samples were taken in a 1m2 area. Collected invertebrates were counted and identified to order or family.
Plant community response to fertilization at Sapelo Island, Georgia
Parallel fertilization experiments were performed in five different types of perennial plant mixtures found in the salt marsh habitat around Sapelo Island, Georgia, from May 1996 to September 1997. Each mixture differed in plot elevation, soil water content, and soil salinity, so each was considered a separate habitat. The mixtures also occurred in different geographic locations (i.e. Dean Creek on southern Sapelo Island, Marsh Landing on southwestern Sapelo Island, and Shell Hammock, which is near the University of Georgia Marine Institute). In May 1996, 16 1mx1m plots were placed within each plant mixture and alternate plots were assigned to control and fertilization treatments. Pelletized fertilizer (29% N, 3% P, 4% K) was broadcast into fertilization treatment plots by hand at the rate of 60g/m^2 every 2 weeks. The central 0.5mx0.5m of each plot was harvested in September 1997 after two summers growth. Live plants were sorted to species, dried to a constant mass, and weighed to measure biomass. Standing dead shoots and litter were not weighed.
Litter chemistry and masses for the MELNHE NxP fertilization experiment
The Multiple Element Limitation in Northern Hardwood Ecosystems (MELNHE) project studies N, P, and Ca acquisition and limitation of forest productivity through a series of nutrient manipulations in northern hardwood forests. This data set includes litterfall chemistry and mass for litter collected approximately weekly through the fall litterfall season, either composited over the entire fall season or selected from individual collection times, pre-treatment (2009), and post-treatment (2012, 2014, 2016, 2018). Additional detail on the MELNHE project, including a datatable of site descriptions and a pdf file with the project description and diagram of plot configuration can be found in this data package: https://portal.edirepository.org/nis/mapbrowse?scope=knb-lter-hbr&identifier=344 These data were gathered as part of the Hubbard Brook Ecosystem Study (HBES). The HBES is a collaborative effort at the Hubbard Brook Experimental Forest, which is operated and maintained by the USDA Forest Service, Northern Research Station.
Soil volumetric water content calculated from neutron hydroprobe data along the LTER-I transects (control and fertilized) at the Jornada Basin LTER, 1986-ongoing
This data package contains volumetric water content (VWC) measurements calculated from soil neutron hydroprobe data collected on the permanent LTER-I transects located at Chihuahuan Desert Rangeland Research Center (CDRRC) in the Jornada Basin of southern New Mexico, USA. The control and treatment transects are parallel to each other and are 2.7 km in length extending from the middle of the College Playa to the foot of Mt. Summerford. The treatment transect was treated annually with ammonium nitrate fertilizer (NH4NO3 at 10g N/m2/yr) until 1987. Measurement stations are located at 30 meter intervals along each transect, and there are neutron probe access tubes located every station on the control transect (n=89) and at every fifth station at the treatment transect (n=19). Measurements were taken at 5 depths using a neutron probe (CPN Model 503DR Hydroprobe) and were then converted to VWC at 30 cm, 60 cm, 90 cm, 110 cm, and 130 cm depths. Neutron probe VWC readings taken in 3 non-weighing mini-lysimeters along each transect are also included. This dataset consists of the calculated water content (cm3 water/cm3 soil) obtained by applying site-specific calibration equations to data derived from the thermalized neutron counts found in EDI packageID knb-lter-jrn.210001001. Measurements were taken at 2 week intervals from April 1982 to 1987 and monthly thereafter. Data collection for this study is ongoing.
Soil water content under rainfall manipulation and nitrogen fertilization treatments at the Jornada Basin LTER site, 2007-2009
This data package contains soil water content data from a precipitation and nitrogen manipulation experiment conducted on the Jornada Experimental Range from 2006-2009. The objective of the study was to understand the interaction of precipitation and nitrogen dynamics on ANPP legacies. Rain-out shelters were used to create 5 levels of precipitation: 80% reduced, 50% reduced, ambient control, 50% increased, and 80% increased precipitation. Half of the plots were randomly assigned to receive ammonium nitrate fertilizer. In a subset of the plots, volumetric soil water content was monitored at shallow (0-5 cm) and deep (30-50 cm) depths. This data set contains the date of collection, block number, plot number, 2007-2008 precipitation treatment, 2009 precipitation treatment, nitrogen treatment, depth of soil probe, sensor voltage, and volumetric water content. Monitoring of soil moisture for this study was discontinued in November 2009. Also available are plant cover data from this study in data package knb-lter-jrn.210278002. For further information and results, see: Throop, H., L. G. Reichmann, O. Sala, and S. Archer. (2012), Response of dominant grass and shrub species to water manipulation: an ecophysical basis for shrub invasion in a Chihuahuan desert grassland. Oecologia 169: 373-383. https://doi.org/10.1007/s00442-011-2217-4 Reichmann, L. G., O. E. Sala, and D. P. C. Peters. (2013), Water controls on nitrogen transformations and stocks in an arid ecosystem. Ecosphere 4(1):11. https://doi.org/10.1890/ES12-00263.1
N and P fertilization experiment net primary productivity data for South of saddle, 1991 - 1997.
A nutrient amendment experiment was initiated in 1990 in 2 alpine plant communities, dry and wet meadow, to determine whether N and/or P limit primary production of these communities, the plants' functional response to increased nutrients, and the community structure and composition responses to changes in nutrient availability.
N fertilization experiment plant species composition data for north of the saddle from 2011 to ongoing, yearly
In August 2010, N fertilization plots were established over a gradient of Geum rossii (Aco) and Deschampsia caespitosa (Des) cover in an area of moist-wet meadow alpine tundra north of the Saddle on Niwot Ridge. A total of 20 plots were set up, with 1X-10X being control plots where no nitrogen (N) was added, and 1N-10N being fertilization plots where N was added. Control and fertilized plots were paired so that pairs had the same percent cover of Aco and Des. Initially in 2010, N addition plots were fertilized at a rate of 28.8 g N x m^2 x yr^-1 (72 g of slow-release Osmocote (40-0-0)). In 2011 fertilization rate was reduced to N x m^2 x yr^-1 (25 g Osmocote (2011-2013), Duration Urea 120, 40-0-0, SGN 150 (2014-on).
Marsh surface elevation data in fertilization plots of a Spartina alterniflora-dominated salt marsh at Goat Island, North Inlet, Georgetown, SC.
A Surface Elevation Table (SET) is used to measure changes in the elevation of the marsh platform in fertilization plots of a Spartina alterniflora-dominated marsh on the Goat Island, North Inlet, Georgetown, SC.
Macroinfaunal counts, 2003-2020, from marsh cores along Rowley River tidal creeks associated with long term fertilization experiments, Rowley, MA.
Macroinfaunal counts, 2003-2006, 2007, 2009, 2012--2020 from marsh cores along Rowley River tidal creeks associated with long term fertilization experiments, Rowley, MA. The long term fertilization experiment, TIDE project, aims to simulate eutrophication on a large scale by the addition of NO3- aiming to reach 70μM concentrations from May to September every year during the growing season. This fertilization of the marsh has been going on at Sweeney Creek since the 2004 growing season and at Clubhead Creek in 2005 and then from 2009 till 2016. Fertilization stopped in 2016.
Snail, Melampus bidentatus, length and biomass measurements for Rowley River tidal creeks associated with long term fertilization experiments, Rowley and Ipswich, MA.
Melampus bidentatus (coffee bean snail) length and biomass measurements for Rowley River tidal creeks associated with long term fertilization experiments, Rowley and Ipswich, MA. The TIDE project aims to simulate eutrophication on a large scale by the addition of NO3- aiming to reach 70μM concentrations from May to September every year during the growing season. This fertilization of the marsh has been going on at Sweeney Creek since the 2004 growing season through 2016 and at Clubhead Creek in 2005 and from 2009 till 2016. Invertebrates were sampled after fertilization ended to look at recovery.
Marsh plant species percent cover for Rowley River tidal creeks associated with long term fertilization experiments, Rowley and Ipswich, MA.
Marsh plant species percent cover for Rowley River tidal creeks associated with long term fertilization experiments, Rowley and Ipswich, MA. The TIDE project aims to simulate eutrophication on a large scale by the addition of NO3- aiming to reach 70µM concentrations from May to September every year during the growing season. This fertilization of the marsh has been going on at Sweeney Creek since the 2004 growing season through 2011 and at Clubhead Creek in 2005 and from 2009 till 2011.
Marsh plant species C:H:N data for Rowley River tidal creeks associated with long term fertilization experiments, Rowley and Ipswich, MA.
Marsh plant species C:H:N data for Rowley River tidal creeks associated with long term fertilization experiments, Rowley and Ipswich, MA. The TIDE project aims to simulate eutrophication on a large scale by the addition of NO3- aiming to reach 70µM concentrations from May to September every year during the growing season. This fertilization of the marsh has been going on at Sweeney Creek since the 2004 growing season through 2011 and at Clubhead Creek in 2005 and from 2009 till 2011.
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