Find research datasets worth reusing

opencc-zeroMay 2022View details →

No evidence of bacterial symbionts influencing host specificity in Aphis gossypii Glover (Hemiptera: Aphididae)

The cotton-melon aphid, Aphis gossypii Glover, is a polyphagous insect pest with many host-specialized biotypes, such as Cucurbitaceae- and Malvaceae-specialized (CU and MA) biotype. Bacterial symbionts were reported to determine host range in some aphids. Whether this is the case in A. gossypii remains unknown. Here, we tested host specificity of CU and MA biotype and compared host specificity between wingless and winged morph within the same biotype, and analyzed the composition of bacterial symbionts. The reproduction of CU and MA biotype reduced by 66.67% and 82.79% respectively on non-native hosts, compared with that on native hosts. The composition of bacterial symbionts was not significantly different between CU and MA biotype, with Buchnera abundance >95% in both biotypes. While, winged morph produced significantly more nymphs than wingless morph on non-native hosts, and<a name="OLE_LINK1"></a> Buchnera abundance in winged morph was only about 10% of that in wingless morph. There seemed to be a relationship between Buchnera abundance and host specificity. We regulated Buchnera abundance by temperature and antibiotics, but did not find that low Buchnera abundance resulted in high reproduction on non-native hosts. We conclude that host specificity of A. gossypii is not controlled by specific bacterial symbionts or by Buchnera abundance.

opencc-zeroJun 2022View details →

Cold tolerance of Trachymyrmex fungus gardening ants and Leucocoprinus symbionts

Symbionts can have profound effects on host fitness, adaptations, and range distributions. Stress-induced evolution is difficult to show in obligate symbioses, however, adaptive evolution within an obligate symbiosis can be investigated experimentally or by correlating trait variation with stress along an ecological cline (i.e., temperature-stress gradient). We investigated the cold-tolerance of the fungus-growing ant Trachymyrmex septentrionalis by performing cold-tolerance assays comparing two populations collected from either the southernmost range of their distribution (Bastrop, TX) or from a site that is approximately 600 km further north (Norman, Oklahoma). We first compared isolated fungal symbionts grown on artificial media to determine cold-tolerance of fungus alone. Subsequently, we conducted cross-fostering experiments between northern and southern host and symbionts to test for synergisms between the partners in generating adaptations of cold tolerance. Ants of the northern fungal populations were more cold-adapted then southern fungal populations. Northern nests were deeper and northern colonies initially rejected fungi from the southern population. The cross-fostering experiments demonstrated that only one partner must be cold tolerant to confer maximum cold-tolerance to the ant-fungus symbiosis, because northern ants growing southern fungus under cold stress performed just as well as northern ants growing northern fungi. Our results suggest that cold stress has been an important selective factor during the migration of this ant-fungus symbiosis into northern latitudes during the last 10,000 years, and that cold tolerance likely is an energetically demanding trait that may be traded off with other aspects of the symbiosis' life history. The symbiosis also appears to have evolved several additional adaptations that increase survival in cold environments, such as building deeper nests that insulate the fungi from cold surface

opencc-by-4.0Jun 2022View details →

Still standing: the heat protection delivered by a facultative symbiont to its aphid host is resilient to repeated thermal stress

This is the dataset for the article "Still standing: the heat protection delivered by a facultative symbiont to its aphid host is resilient to repeated thermal stress". This experimental study focused on the heat-protective mutualism between the pea aphid Acyrthosiphon pisum and the facultative bacterium Serratia symbiotica. We exposed two aphid lines (5A: deprived of S. symbiotica, 5AR: infected by S. symbiotica) to a varying number of heat shocks (35°C for 2 h) applied during insect immature development: none (control), one, two, or three. We recorded aphid immature survival rate, total longevity, immature development time, lifetime fecundity and hind tibia length (body size). . Individual = identification of the individual . Line = aphid clonal line, either deprived of (5A) or infected by S. symbiotica (5AR) . Temp = thermal treatment, defined by the number of heat shocks applied during aphid juvenile life (0 = control, 1, 2 or 3) . Survival = whether (Y) or not (N) the individual survived until reproduction  . Adulthood = time elapsed between birth and first reproduction (in days) . Longevity = time elapsed between birth and death (in days) . Fecundity = total number of larvae produced by each aphid throughout lifetime . Wing = whether (Y) or not (N) the individual developed wings following imaginal moult . Tibia = hind tibia length as a proxy of body size (in mm)

opencc-zeroSep 2022View details →

RNA-seq of green hydra strains' ( Hydra viridissima) response to the removal or exchange of symbionts

The symbiotic hydra Hydra viridissima has a stable symbiotic relationship with the green alga Chlorella. This hydra appears to cospeciate with the symbiotic alga, and some strains are known to have strain-specific host/symbiont combinations. To investigate the mechanism of the specificity between host and symbiont, we explored the effect of the removal or exchange of symbionts in two distantly related H. viridissima strains (K10 and M9). In this study, we compared the gene expression of symbiont-removed, symbiont-exchanged hosts for each strain. The data include the raw read fastaq, assembled sequences, and read counts of RNA-seq. We also attached the results of differential gene expression analyses for all combinations of the hosts.

opencc-by-4.0Sep 2017View details →

Metagenomic data for Bathymodiolus symbionts deposited in IMG (2017)

Metagenomic data for the sulfur- and methane-oxidizing symbionts of Bathymodiolus mussels and different sponge species deposited in the Integrated Microbial Genomes (IMG) database of the DOE Joint Genome Institute (http://img.jgi.doe.gov/) until October 2017.

opencc-by-4.0Sep 2017View details →

Metagenomic data for gutless oligochaete symbionts (2017)

This list contains accession numbers to: -Metatranscriptomic data of gutless oligochaete Olavius algarvensisworms depositied in European Nucleotide Archive ENA -Metaproteomic data of gutless oligochaete Olavius algarvensisworms deposited in proteomics data repositories -Draft genomes of endosymbionts from various gutless oligochaete hosts, in submission process. If you are interested in these datasets, please let us know.

opencc-zeroApr 2024View details →

Data from: A theoretical model for host-controlled regulation of symbiont density

There is growing empirical evidence that hosts (such as insects and corals) actively control the density of their mutualistic symbionts according to their requirements. Such active regulation can be facilitated by compartmentalisation of symbionts within host tissues, which confers a high degree of control of the symbiosis to the host. Here, we build a general theoretical framework to predict the underlying ecological drivers and evolutionary consequences of host-controlled endosymbiont density regulation for a mutualistic association between a host and a compartmentalised, vertically transmitted symbiont. Building on the assumption that the costs and benefits of hosting a symbiont population increase with symbiont density, we use state-dependent dynamic programming to determine an optimal strategy for the host, i.e., that which maximises host fitness, when regulating the density of symbionts. Simulations of active host-controlled regulation governed by the optimal strategy predict that the density of the symbiont should converge to a constant level during host development, and following perturbation. However, a similar trend also emerges from alternative strategies of symbiont regulation. The strategy which maximises host fitness also promotes symbiont fitness compared to alternative strategies, suggesting that active host-controlled regulation of symbiont density could be adaptive for the symbiont as well as the host. Adaptation of the framework allowed the dynamics of symbiont density to be predicted for other host-symbiont ecologies, such as for non-essential symbionts, demonstrating the versatility of this modelling approach.

opencc-zeroMay 2024View details →

Data from: Symbiont infection and psyllid haplotype influence phenotypic plasticity during host switching events

Many herbivorous insect species exhibit phenotypic plasticity when using multiple hosts, which facilitates survival in heterogeneous host environments. Physiological host acclimation is an important part of it, yet the effects of host acclimation on insect feeding behavior are not well studied, particularly for insect vectors of plant pathogens. We studied the combined effects of host acclimation and infection with a plant pathogenic symbiont on feeding behavior of Bactericera cockerelli, an oligophagous psyllid widespread in both crop and natural habitats that feeds primarily on Solanaceae and transmits an economically important plant pathogen, Candidatus Liberibacter solanacearum (CLso). We used a factorial design and the electrical penetration graphing technique to disentangle the effects of host acclimation, CLso infection, and psyllid haplotype on the within-plant feeding behavior of B. cockerelli during conspecific and heterospecific host switches. This approach allows to connect phenotypic plasticity with the role of B. cockerelli as a vector by quantifying the frequency and duration of behaviors involved in CLso transmission. We found significant reductions in multiple metrics of B. cockerelli feeding efficiency, exacerbated by infection with CLso, which could lead to reduced transmission of this pathogen. Psyllid genotype was also important; the Central haplotype exhibited less dramatic changes in feeding efficiency than the Western haplotype during heterospecific host switches. Our study shows that host acclimation and heterospecific host switching directly alter feeding behaviors underlying pathogen transmission, and that the magnitude of feeding efficiency reductions depends on both host genotype and infection status.

opencc-zeroJun 2024View details →

Symbionts out of sync: decoupled physiological responses are widespread and ecologically important in lichen associations

A core vulnerability in symbioses is the need for coordination between the symbiotic partners, which are often assumed to be closely physiologically integrated. We critically re-examine this assumed integration in lichen symbioses, recovering a long overlooked yet fundamental physiological asymmetry in carbon balance. We examine the physiological, ecological and transcriptional basis of this asymmetry in the lichen Evernia mesomorpha. This carbon balance asymmetry depends on hydration source and aligns with climatic range limits. Differences in gene expression across the E. mesomorpha symbiosis suggest that the physiologies of the primary lichen symbionts are decoupled. Furthermore, we use gas-exchange data to show that asymmetries in carbon balance are widespread and common across evolutionarily disparate lichen associations. Using carbon balance asymmetry as an example, we provide evidence for the wide-ranging importance of physiological asymmetries in symbioses.

opencc-by-4.0Jul 2024View details →

Figure 6 in New host detection of the parasitic mite, Erythraeus pistacicus (Trombidiformes: Erythraeidae) from Iran and indication of possible infection with bacterial symbionts

Figure 6. Haplotype network of Cardinium endosymbionts based on 16S rDNA sequences.

opencc-by-4.0Jul 2024View details →

Figure 4 in New host detection of the parasitic mite, Erythraeus pistacicus (Trombidiformes: Erythraeidae) from Iran and indication of possible infection with bacterial symbionts

Figure 4. Haplotype network of Wolbachia endosymbionts based on wsp gene.

Supplement to "Sulfur-oxidizing symbionts without canonical genes for autotrophic CO2 fixation"

Supplementary material to accompany the article "Sulfur-oxidizing symbionts withoutcanonical genes for autotrophic CO2 fixation"

Supplementary Table 11 for "Sulfur-oxidizing symbionts without canonical genes for autotrophic CO2 fixation"

Direct protein stable isotope fingerprinting (SIF) values for Kentron sp. H. δ13C values were offset-corrected using a human hair standard for each instrument run. Full raw and processed data are available from the PRIDE repository (https://www.ebi.ac.uk/pride/archive/) with the dataset identifier PXD011616.

Supplementary Table 10 for "Sulfur-oxidizing symbionts without canonical genes for autotrophic CO2 fixation"

Number of genomes with each predicted metabolism in the IMG/ER database, based on the presence/absence of key genes. Except where noted, the number of genomes was not filtered for genome completeness.

Supplementary Table 8 for "Sulfur-oxidizing symbionts without canonical genes for autotrophic CO2 fixation"

Hypothetical reaction scheme that can allow autotrophic CO2 fixation with enzymes that are predicted in Kentron genomes. Free energy values (ΔrG'm) were calculated for pH 7.0 and concentrations 1 mM using eQuilibrator (http://equilibrator.weizmann.ac.il)

Supplementary Table 5 for "Sulfur-oxidizing symbionts without canonical genes for autotrophic CO2 fixation"

List of Transporter Classification families of energy-dependent organic substrate uptake transporters.

Supplementary Table 6 for "Sulfur-oxidizing symbionts without canonical genes for autotrophic CO2 fixation"

Genomes of basal Gammaproteobacteria used for phylogenetic analysis and comparison of organic uptake transporter content. References are to genome description, if published, otherwise to author and date of data deposition. Taxonomy based on LPSN (http://www.bacterio.net), if available. Accession numbers are for INSDC contig sets or assemblies unless otherwise indicated.

Supplementary Table 3 for "Sulfur-oxidizing symbionts without canonical genes for autotrophic CO2 fixation"

Key enzymes for autotrophic pathways, and enzymes of reference set used for comparison of read mapping vs SwissProt database.

Supplementary Table 2 for "Sulfur-oxidizing symbionts without canonical genes for autotrophic CO2 fixation"

Summary statistics of Kentrongenome assemblies. Completeness, contamination, and strain heterogeneity values were estimated with conserved set of marker genes for Gammaproteobacteria using the CheckM pipeline.

Supplementary Table 1 for "Sulfur-oxidizing symbionts without canonical genes for autotrophic CO2 fixation"

Collection localities and dates for Kentrophoros metagenomics and transcriptomics samples.