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Sawgrass above ground biomass from the Taylor Slough, just outside Everglades National Park (FCE), South Florida from October 1997 to December 2006
Sawgrass biomass is measured for TS/Ph-1, 2, 3, & 6 since 1999; TS/Ph-4 & 5 since 1997 to 2006; SRS-1b, 2, & 3 since 2000; SRS1c for 2005 to 2006, and SRS1d since 2006. Three 1 m2 plots are placed on each site. The sites are measured every two months. The number of sawgrass plants is counted for each 1 m2 plot and one third or a minimum of fifteen plants is measured. For each measured plant, measurements of the total number of live leaves, length of the live leaves, and culm diameter at the base are taken. From these measurements we calculate the average leaf length, and the sum of the length of the leaves. We created a model for biomass using a stepwise regression to see which of the variables measured and calculated are more correlated to explain plant biomass. Obtaining the mean biomass for the plants within the plot and multiplying it by the number of plants counted in the plot calculates biomass for each plot. The total biomass for each plot is summed with the other two plots in the same site, and they are averaged to obtain one biomass number per site. To validate our model, plant clippings are obtained in which four plants are clipped (small, medium, large, inflorescence) from each site. The same measurements are applied to the clipped plants as those applied to the measured plants in the plots. The plant clippings are oven dried at 70 degrees C and weighed.
Precipitation from the Taylor Slough, just outside Everglades National Park (FCE), South Florida from August 2000 to December 2006
Rain level is recorded at least every thirty minutes at TS/Ph4 and TS/Ph5 (data since 2000). Rain level data are collected using ISCO rain level gages attached to ISCO autosamplers, which also act as dataloggers. We condense these data into daily cumulative rainfall, although hourly rain level data are also available.
Water Levels from the Taylor Slough, just outside the Everglades National Park (FCE), South Florida from October 1997 to December 2006
Water level is recorded at least hourly at TS/Ph4 and TS/Ph5. Water level is measured with acoustic water level gages that digitally record relative water height relative to the local soil surface.
Groundwater and surface water phosphorus concentrations, Everglades National Park (FCE), South Florida for June, July, August and November 2003
Seawater intrusion into a coastal aquifer mixes with the discharging fresh water to form a zone of mixed composition. This mixing zone is considered to be geochemical important in a carbonate aquifer as an area of enhanced carbonate mineral dissolution and,or precipitation. Ion exchange reactions are also common within the mixing zone as the dominant cation in seawater, sodium, replaces other ions adsorbed to the aquifer matrix. Phosphorus has a strong affinity for adsorption to calcium carbonate minerals. Both the dissolution of calcium carbonate minerals as well as ion exchange reactions have the potential to release phosphorus from the aquifer matrix to the groundwater. Discharge of this phosphorus-laden groundwater, as induced by the fresh/saline water interface, may then be an additional source of phosphorus to the overlying coastal environments. Both surface water and groundwater were collected across the seawater intrusion zone of the coastal Everglades in south Florida during the summer of 2003. Hydrogen sulfide was released from the groundwater wells during sampling, indicating the groundwater was most likely anoxic. In order to reduce the potential exposure of the groundwater to oxygen during sampling, groundwater samples were collected in the following manner. Groundwater wells were first purged of at least three well volumes prior to sampling. Water samples were then collected using a submersible pump with a 16-gauge needle fitted at the end of the discharge hose. The needle was pushed through a rubber stopper covering an acid-washed vacutainer that was first flushed with nitrogen gas to remove and oxygen, then evacuated with a vacuum pump to establish a negative pressure within the vacutainer. Water samples were stored on ice and transported to the laboratory. Samples were processed for total phosphorus using colorimetery following dry-oxidation/acid hydroloysis methods within 1 to 5 days following sample collection. Salinity of the groundwater and surf
Water Temperature measured at Shark River, Everglades National Park (FCE) from October 2007 to August 2008
American alligators (Alligator mississippiensis) are top predators in the Florida Coastal Everglades (FCE), but their movement patterns and role in the ecosystem are largely unknown. Proposed restoration and management efforts in the Everglades are likely to influence both alligator movements and the spatiotemporal patterns of their effects on ecosystem and community dynamics, making studies of the factors influencing alligator movements important at this time. In October 2007, we initiated a study of alligators in the Shark River Slough of Everglades National Park using two tracking techniques that have yet to be used on crocodilians: GPS tracking and passive acoustic telemetry. GPS units attached to two alligators have provided 120 positions over a two month period while acoustic transmitters attached to 12 alligators have been detected 34,000 times on an array of 8 monitoring stations within the river system. GPS positions are more accurate than acoustic monitoring and can provide data over the entire range an alligator might move, but acoustic transmitters provide more temporally detailed information on movements within a monitoring array and over a longer duration (potentially years rather than months). Our preliminary results show that alligators may remain in localized areas for weeks at a time, but make long-distance moves from the mouth of the river system to the marsh-mangrove ecotone and likely into the marsh. In general, most alligators moved upstream as freshwater inputs decreased, but some individuals remained near the river mouth even when salinities were high. Ultimately, by characterizing alligator movements in relation to spatiotemporal variation in environmental factors we will be able to predict how alligator distributions and populations will respond to planned increases in freshwater flow. Furthermore, movement data combined with dietary and stable isotopic studies may shed light on the possible role of alligators in the redistribution of nutri
Water Temperature, Salinity and other physical measurements taken at Shark River, Everglades National Park (FCE LTER) from February 2010 to March 2014
In early 2010 we established three new permanent water quality monitoring stations in the Shark River Estuary. These stations expanded FCE's spatial coverage in terms of salinity and also established the first continuous dissolved oxygen monitoring in the estuary.
Water Temperature measured at Shark River, Everglades National Park (FCE) from July 2007 to June 2011
Between 2007 and 2011 we placed HOBO continuous water temperature loggers at 17 locations in the Shark River Estuary for various lengths of times. These stations expanded FCE's spatial coverage in terms of temperature monitoring in the estuary.
Nutrient data from the Peat Collapse-Saltwater Intrusion Field Experiment from brackish and freshwater sites within Everglades National Park, Florida (FCE LTER), collected from October 2014 to September 2016
With sea level rise increasing, saltwater intrusion into low-lying coastal wetlands is likely to occur. We simulated saltwater intrusion into an Everglades marsh through monthly additions of elevated salinity water. Monthly porewater nutrients were taken at 15 cm depth from a brackish and a freshwater marsh. Porewater physicochemistry was measured 24 hours after dosing. Collection occurred from Oct 2014 - Sep 2016. The collected water was then analyzed for temperature, conductivity, salinity, pH, alkalinity, chloride, DOC, NH4, SO4, TDN, SRP, TDP, and sulfide. These data are published in Wilson, B.J., Servais, S., Mazzei, V., Davis, S.E., Kelly, S., Gaiser, E., Kominoski, J.S., Richards, J., Rudnick, D., Sklar, F., Stachelek, J., and Troxler, T.G. Salinity pulses interact with seasonal dry-down to increase ecosystem carbon loss in marshes of the Florida Everglades. 2018. Ecological Applications 28:2092-2018.
Leaf nutrient and root biomass data from the Peat Collapse-Saltwater Intrusion Field Experiment within Everglades National Park (FCE), collected from October 2014 to September 2016
With sea level rise increasing, saltwater intrusion into low-lying coastal wetlands is likely to occur. We simulated saltwater intrusion into an Everglades marsh through monthly additions of elevated salinity water. Yearly sawgrass leaf carbon, nitrogen, and phosphorus concentrations and live root biomass measurements were measured from a brackish water and freshwater marsh. All measurements were taken every other month 24 hours after dosing. Measurements occurred from Oct 2014 - Sep 2016. Ecosystem flux measured includes gross ecosystem production, ecosystem respiration of CO2, net ecosystem production, and ecosystem respiration of CH4. These data are published in Wilson, B.J., Servais, S., Mazzei, V., Davis, S.E., Kelly, S., Gaiser, E., Kominoski, J.S., Richards, J., Rudnick, D., Sklar, F., Stachelek, J., and Troxler, T.G. Salinity pulses interact with seasonal dry-down to increase ecosystem carbon loss in marshes of the Florida Everglades. Ecological Applications. Accepted.
Biomass data from the Peat Collapse-Saltwater Intrusion Field Experiment within Everglades National Park (FCE), collected from October 2014 to September 2016
With sea level rise increasing, saltwater intrusion into low-lying coastal wetlands is likely to occur. We simulated saltwater intrusion into an Everglades marsh through monthly additions of elevated salinity water. Monthly biomass, aboveground net primary production, and culm density measurements were measured from a brackish water and freshwater marsh. All measurements were taken every other month 24 hours after dosing. Measurements occurred from Oct 2014 - Sep 2016. Ecosystem flux measured includes gross ecosyetem production, ecosystem respiration of CO2, net ecosystem production, and ecosystem respiration of CH4. These data are published in Wilson, B.J., Servais, S., Mazzei, V., Davis, S.E., Kelly, S., Gaiser, E., Kominoski, J.S., Richards, J., Rudnick, D., Sklar, F., Stachelek, J., and Troxler, T.G. Salinity pulses interact with seasonal dry-down to increase ecosystem carbon loss in marshes of the Florida Everglades. Ecological Applications. Accepted.
Modeled flux data from the Peat Collapse-Saltwater Intrusion Field Experiment within Everglades National Park (FCE), collected from October 2014 to September 2016
With sea level rise increasing, saltwater intrusion into low-lying coastal wetlands is likely to occur. We simulated saltwater intrusion into an Everglades marsh through monthly additions of elevated salinity water. Monthly modeled ecosystem flux measurements were calculated from a brackish water and freshwater marsh. Ecosystem flux was measured 24 hours after dosing. Measurements occurred from Oct 2014 - Sep 2016. Ecosystem flux measured includes gross ecosyetem production, ecosystem respiration of CO2, net ecosystem production, and ecosystem respiration of CH4. These data are published in Wilson, B.J., Servais, S., Mazzei, V., Davis, S.E., Kelly, S., Gaiser, E., Kominoski, J.S., Richards, J., Rudnick, D., Sklar, F., Stachelek, J., and Troxler, T.G. Salinity pulses interact with seasonal dry-down to increase ecosystem carbon loss in marshes of the Florida Everglades. Ecological Applications. Accepted.
Flux data from the Peat Collapse-Saltwater Intrusion Field Experiment within Everglades National Park, collected from October 2014 to September 2016
With sea level rise increasing, saltwater intrusion into low-lying coastal wetlands is likely to occur. We simulated saltwater intrusion into an Everglades marsh through monthly additions of elevated salinity water. Monthly ecosystem flux measurements were taken from a brackish water and freshwater marsh. Ecosystem flux was measured 24 hours after dosing. Measurements occurred from Oct 2014 - Sep 2016. Ecosystem flux measured includes gross ecosyetem production, ecosystem respiration of CO2, net ecosystem production, and ecosystem respiration of CH4. These data are published in Wilson, B.J., Servais, S., Mazzei, V., Davis, S.E., Kelly, S., Gaiser, E., Kominoski, J.S., Richards, J., Rudnick, D., Sklar, F., Stachelek, J., and Troxler, T.G. Salinity pulses interact with seasonal dry-down to increase ecosystem carbon loss in marshes of the Florida Everglades. Ecological Applications. Accepted.
Community structure of aquatic vertebrates and macroinvertebrates in relation to hydrologic variables in the Shark River Slough, Everglades National Park, Florida USA: Ongoing since 2012
The purpose of this project is to characterize the community structure of aquatic fauna (macroinvertebrates and fishes) and floating mats (including periphyton) across the heterogenous landscape of the NE Shark River Slough in Everglades NP. The community structure (abundance, biomass, and species composition) of floating mat, emergent vascular plants, fishes, and macroinvertebrates, and the spatial distribution of the latter in relation to hydrology, are among the key performance measures targeted for monitoring. The aim of our study is to provide a baseline characterization of these parameters so that the impact of future management activities (e.g., Modified Water Deliveries and Comprehensive Everglades Restoration Plan) can be assessed. The study area is sampled during the late wet season (September through November) to capture data when all sites are innundated with water and aquatic animal standing stocks are typically at their maximum. We document landscape-scale patterns in the NE Shark River Slough from this comprehensive sampling.
Decomposition rates of four litter types along coastal gradients in Everglades National Park (FCE LTER), Florida, USA: 2020-2021
Leaf litter, of variable quality, was deployed along freshwater-to-marine gradients to investigate the drivers of litter breakdown in the Florida Coastal Everglades. Sea-level rise provides both stressors and subsidies to microbial communities that break down litter, and is also causing shifts in the vegetation producing litter, changing the initial quality of litter being deposited in coastal wetlands. The goal of this project was to understand the complex relationship between marine stressors and subsidies, by performing a reciprocal transplant of litter. Litter from each major vegetation species along the freshwater to marine gradient were deployed at sites in freshwater, the ecotone, mangrove forest, and in Florida Bay. This reciprocal transplant of different qualities of leaf litter into novel environments across a gradient of salinity and phosphorus will give insights into the effects of litter carbon quality, littery chemistry, microbial productivity of decomposers, and environmental chemistry as drivers of the breakdown of leaf litter.
A database of soil type and soil depth across Everglades National Park, Florida, USA
This dataset contains record of soil type and soil depth across Everglades National Park. This data was obtained through published literature, unpublished field data, and inter-agency collaboration. Data come from either soil cores to bedrock or refusal (as specified by data source), cores that did not reach bedrock, a soil probe to determine depth, or the depth of installed surface elevation tables. Soil type was classified either along a gradient of organic matter content when data was available: mineral (<40%), intermediate (40-70%), or organic (<70%). When data on organic matter content wasn’t available but qualitative descriptions of soil type were, the type unit of soil was classified as either organic or mineral based on the descriptive lithology. When lithologic descriptions were provided and indicated the soil profile was not entirely one soil type (e.g. peat), depth of peat within core was recorded. When data source had specific coordinates, those coordinates were used. For older studies that only provided a figure showing the location of each estimate at a broad scale, location was estimated by georeferencing the figure in ArcMap, and dropping a point at each location. Records were cross-referenced with a recent vegetation map developed by the National Park Service in order to obtained vegetation community at each location (Ruiz et al. 2017).
Fatty acid data among habitats and between seasons in Everglades National Park, Florida, USA, 2018-2019
Fatty acid profiles to represent the aquatic food web of the freshwater habitats from wet and dry seasons of the Everglades, Florida, USA. This dataset provides fatty acid profiles for a range of food-web members from basal resources (vascular plants, benthic flocculent matter, periphyton), aquatic invertebrates, amphibians, and fishes (including non-native and invasive species). Each sample is a composite of six individuals on average. Samples were collected from multiple habitats across sites in Shark River Slough and Taylor Slough in the wet (2018) and dry (2019) seasons. In some cases, in order to have enough tissue to make a composite sample, the composite was made at a higher level then at a habitat within a site. For example, some composites are from the same slough-season-habitat and combined from multiple sites. The goal was to capture as much of the aquatic food web in the wet and dry seasons as possible to build fatty-acid derived food-web snap shots, not necessarily to quantify variation at various levels of different factors (e.g., habitat and site). However, common species are relatively well replicated among factor levels. Data collection is complete.
Inventory of soil prokaryotic microbiome (via 16S based on rRNA gene amplicons) in freshwater and brackish water marshes following saltwater intrusion along Shark River Slough boundary, Everglades National Park (FCE LTER), Florida, USA, September 2018
Global sea-level rise is transforming coastal ecosystems, especially freshwater wetlands, in part due to increased episodic or chronic saltwater exposure, leading to shifts in microbial communities and related ecological services. Soil prokaryotes play a fundamental role in regulating important biogeochemical processes in coastal wetland ecosystem. Yet, it is still difficult to predict how soil prokaryotic communities respond to the saltwater exposure because of poorly understood prokaryotic sensitivity within complex wetland soil microbial communities, as well as the high heterogeneity of wetland soils and saltwater exposure. To address this, a four-year experimental simulation of saltwater intrusion in a pristine freshwater site and a previously saltwater-impacted site was conducted. The saltwater addition started in October 2014 on a monthly basis and continued through October 2018. The dataset contains amplicon sequencing date of 16S rRNA gene obtained from saltwater-exposed soils and unmanipulated native soils in both sites (collected in September 2018). The 2018 data are published in Zhao et al. 2023. A detailed list of sequence data and their accession numbers in GenBank is provided, and data collection is complete. This data package is an inventory of sequence read archive (SRA) entries available through GenBank BioProject PRJNA804545 (https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA804545). This data package is associated with the following publication: Zhao, J., Chakrabarti, S., Chambers, R., Weisenhorn, P., Travieso, R., Stumpf, S., Standen, E., Briceno, H., Troxler, T., Gaiser, E., Kominoski, J., Dhillon, B., & Martens-Habbena, W. (2023). Year-around survey and manipulation experiments reveal differential sensitivities of soil prokaryotic and fungal communities to saltwater intrusion in Florida Everglades wetlands. Science of The Total Environment, 858, 159865. https://doi.org/10.1016/j.scitotenv.2022.159865 Instead of citing this package, which is an
Inventory of soil prokaryotic and fungal microbiome (via 16S rRNA gene amplicons and ITS sequencing) from Shark River Slough and Taylor Slough, Everglades National Park (FCE LTER), Florida, USA, February 2019 - October 2020
Global sea-level rise is transforming coastal ecosystems, especially freshwater wetlands, in part due to increased saltwater exposure, leading to change in soil microbial communities and many important biogeochemical processes. Given the high spatial and temporal heterogeneity in coastal wetlands, especially in tropical or subtropical climates characterized by seasonal temperature, precipitation, and tidal fluctuations, it remains unclear which environmental factors influence the compositions of soil microbial communities in wetlands affected by varying degrees of sea-water intrusion. To address this, a two-year survey was conducted on microbial community structure in submerged surface soils from 14 wetland sites across the Florida Everglades, representing three major ecosystem types, i.e. freshwater marshes, mangrove forests, and seagrass meadows. Bulk surface soil samples of each site were collected from February 2019 to October 2020 to cover dry and wet seasons. In addition to bulk soil samples, soil cores were collected from each site in August 2020 to assess vertical gradients of microbial communities. The dataset contains amplicon sequencing data of 16S rRNA gene (both bulk soil and soil cores) and ITS gene (only the bulk soil). The 2019 to 2020 data are published in Zhao et al. 2023. A detailed list of sequence data and their accession numbers in GenBank is provided, and data collection is complete. This data package is an inventory of sequence read archive (SRA) entries available through GenBank BioProject PRJNA804243 (https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA804243), PRJNA804246 (https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA804246), and PRJNA804228 (https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA804228). This data package is associated with the following publication: Zhao, J., Chakrabarti, S., Chambers, R., Weisenhorn, P., Travieso, R., Stumpf, S., Standen, E., Briceno, H., Troxler, T., Gaiser, E., Kominoski, J., Dhillon, B., & Martens-H
Leaf litter, soil, and periphyton gene expression along freshwater to marine gradients in Everglades National Park (FCE LTER), Florida, USA, January 2021 and April 2021
We collected leaf litter, periphyton, and soil along freshwater to marine gradients at SRS-2, SRS-4, SRS-6, TS/PH-2, TS/Ph-3, TS/Ph-7a, and TS/Ph-10. Samples were collected in January and April of 2021 to understand how microbial communities respond to and influence the breakdown of organic matter along freshwater to marine transects. Data collection for this project is complete. For each site and litter pair we collected a subset of 2-3 g wet mass of litter, a grab sample of soil, and a grab sample of periphyton for each site. All subsamples were preserved at -20°C until extraction, which took place up to a year after initial collection. Samples were sent to Novogene (Novogene Co. Ltd., Beijing, China) for the total RNA extraction followed by metatranscriptome sequencing. We selected n = 12 genes/gene families encoding for focal enzymes to investigate which are important to the breakdown of organic matter: Dioxygenases (associated with aerobic respiration), Sulfatases (associated with the release of sulfates from complex molecules), sulfite reductases (associated with sulfite reduction), methyl coenzyme M reductase and formylmethanofuran (associated with methanogenesis), nitrite reductases (associated with nitrite reduction), cellobiosidase, glucosidase, and xylosidase (associated with cellulose breakdown), phenol oxidase (associated with lignin breakdown), acid phosphatase (associated with phosphate acquisition in acidic environments), and alkaline phosphatase (associated with phosphate acquisition in basic environments). For each gene/family of interest, we searched all annotated transcripts for all entries corresponding to that gene/family and combined all values for a total expression. We selected n = 6 monophyletic microbial functional groups, representing sulfate reducers, sulfate oxidizers, methane oxidizers, methanogens, nitrite oxidizers, and ammonia oxidizers associated with sulfate and methane cycling. We filtered all annotated transcripts for all specie
Annual summaries of daily climatological observations from the National Weather Service weather station at the UGA Marine Institute on Sapelo Island, Georgia for 1958 to 2004
Daily summaries of climatological observations from the National Weather Service weather station on Sapelo Island, Georgia, were obtained from the NOAA National Climatic Data Center (http://www.ncdc.noaa.gov/) covering the period 1958 through 2004. Records for incomplete years with more than one month of missing observations were deleted (i.e. 1964, 1969-1971), then missing values of daily minimum, maximum and mean temperature were estimated by cubic spline interpolation to fill in data gaps of five or fewer consecutive days. Annual summary statistics were then calculated for daily minimum, maximum and mean air temperature and total precipitation.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.