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FIGURE 1 in Automated segmentation of insect anatomy from micro-CT images using deep learning

FIGURE 1 Segmentation pipeline overview. (a) Specimens are placed in iodine for staining for 2 weeks and then placed in small vials containing 99% ethanol to prevent them from moving during scanning. (b) The computed tomography (CT) scanner acquires successive X-ray images of the stepwise rotating specimen, and, using a user-defined reference image, automatically reconstructs them to produce orthogonal cross-section stacks that are used for the volume reconstruction of the specimen. (c) Volume rendering for future morphological studies is performed using Amira software. (d) Semiautomated segmentation of the brain volume of each scan (in orange) using the watershed method in Amira. (e) Schematic representation of the U-Net architecture used as the core of the pipeline for the development of a fully automated brain segmentation method. (f) The acquired brain images are used for training after preprocessing augmentation and manual creation of masks. (g) The network's prediction (in yellow) is postprocessed for smoothing out overpredicted areas (in red).

FIGURE 2 in Automated segmentation of insect anatomy from micro-CT images using deep learning

FIGURE 2 Exemplar images of full-body scans from different ant species. Three-dimensional (3D) reconstructed microcomputed tomography (micro-CT) image of (a) Acromyrmex versicolor and (b) Atta texana worker specimens, using volume rendering in Amira. (c) 2D micro-CT full body image of the Atta texana specimen (original 1000 × 1000 px). The brain area is the area with the most uniform pixel density within the whole body in its stained state, which makes it easy to recognize in most high-quality scans.

FIGURE 3 in Automated segmentation of insect anatomy from micro-CT images using deep learning

FIGURE 3 Example of semiautomated brain image segmentation. The brain area (in orange) of an Atta texana ant specimen was segmented using the watershed method in Amira; the 1000 × 1000 × 1000 px 3D image was manually postprocessed by smoothing and cropping oversegmented areas.

FIGURE 9 in Automated segmentation of insect anatomy from micro-CT images using deep learning

FIGURE 9 Prediction of ganglia in the thorax. As the tissue texture in the image is similar to that of the brain, the network accurately predicts other areas of nervous tissue in the organism. The pixel island detection step isolates the brain, but without this step neural tissue can be isolated.

FIGURE 8 3D in Automated segmentation of insect anatomy from micro-CT images using deep learning

FIGURE 8 3D volume of ant brain reconstructed from 2D images (original 520 × 520 px) predicted by the algorithm. 3D reconstructed brain prediction of an Atta texana worker.

FIGURE 7 in Automated segmentation of insect anatomy from micro-CT images using deep learning

FIGURE 7 Pipeline performance demonstrated both for validation (top row) and testing (bottom row) sets. (a, d) Raw images of head of Acromyrmex versicolor and Carebara atoma ant specimens, cropped along the x-y axes. The manually segmented brain areas are indicated in blue. (b, e) Network predictions before postprocessing (in yellow). Areas in yellow dotted circles are pixel islands not connected to the brain area that were overpredicted. (c, f) Predictions after postprocessing (in red). The borders of the predicted areas show good agreement with the manual segmentation in both sets. Note that in overlapping manually and automatically segmented areas in b, c, e, and f, colors appear green or purple.

Figures 10-12 in A new genus and species of armored scale insect (Hemiptera: Diaspididae) from Australia found in the historic Koebele Collection of the California Academy of Sciences John W. Dooley III

Figures 10-12. Diaspididae spp., habitus, detail of venter of L1 lobes, and pygidium ventral (left) and dorsal (right). 10) Dichosoma convexa (after Brimblecombe 1957). 11) Duplaspidiotus claviger (after Ferris 1937). 12) Eulaingia stenophyllae (after Borchsenius and Williams 1963).

Figure 3 in A new genus and species of armored scale insect (Hemiptera: Diaspididae) from Australia found in the historic Koebele Collection of the California Academy of Sciences John W. Dooley III

Figure 3. Protomorgania koebelei adult female (pygidium). A) L1 lobes fused ventrally, appressed dorsally; B) single simple plate between L1 and position of L2 seta; C) anal pore; D) sclerotized arch; E) chitinized and finely stippled cuticle around vulva.

Figure 2 in A new genus and species of armored scale insect (Hemiptera: Diaspididae) from Australia found in the historic Koebele Collection of the California Academy of Sciences John W. Dooley III

Figure 2. Protomorgania koebelei adult female (thorax and Abdomen). A) anterior perispiracular pores; B) dorsal microducts; C) dorsal microducts, magnified; D) roughened cuticle.

Figure 19 in A new genus and species of armored scale insect (Hemiptera: Diaspididae) from Australia found in the historic Koebele Collection of the California Academy of Sciences John W. Dooley III

Figure 19. Pseudotargionia glandulosa, habitus, detail of venter of L1 lobes, and pygidium ventral (left) and dorsal (right) (after Ferris 1937).

Figure 1. Protomorgania koebelei adult female. A in A new genus and species of armored scale insect (Hemiptera: Diaspididae) from Australia found in the historic Koebele Collection of the California Academy of Sciences John W. Dooley III

Figure 1. Protomorgania koebelei adult female. A) habitus; B) tubercle; C) anterior spiracle; D) posterior spiracle; E) pygidial lobes; F) slide mounted female habitus; G) habitus on host; H) close-up of habitus on host

Figures 7-9 in A new genus and species of armored scale insect (Hemiptera: Diaspididae) from Australia found in the historic Koebele Collection of the California Academy of Sciences John W. Dooley III

Figures 7-9. Diaspididae spp., habitus, detail of venter of L1 lobes, and pygidium ventral (left) and dorsal (right) (after Brimblecombe 1957). 7) Diaphoraspis orbata. 8) Diaspidopus distinctus. 9) Diastolaspis novata.

Figures 4-6 in A new genus and species of armored scale insect (Hemiptera: Diaspididae) from Australia found in the historic Koebele Collection of the California Academy of Sciences John W. Dooley III

Figures 4-6. Diaspididae spp., habitus, detail of venter of L1 lobes, and pygidium ventral (left) and dorsal (right) (after Brimblecombe 1957). 4) Achorphora obliqua. 5) Acontonidia triangulari. 6) Aspidonymus woodwardi.

Figures 16-18 in A new genus and species of armored scale insect (Hemiptera: Diaspididae) from Australia found in the historic Koebele Collection of the California Academy of Sciences John W. Dooley III

Figures 16-18. Diaspididae spp., habitus, detail of venter of L1 lobes, and pygidium ventral (left) and dorsal (right). 16) Neoleonardia extensa (after Ferris 1938). 17) Neomorgania eucalypti (after Ferris 1937). 18) Pseudaonidia duplex (after Ferris 1937).

Fig. 3 in Repatriation of knowledge about insects and types through the DORSA virtual museum (Digital Orthoptera Specimen Access)

Fig. 3: Orthoptera (crickets and grasshoppers) collections in Germany – number of holotypes in major museums. A similar distributed pattern is observed for type material of other groups of organisms housed in German museums or research institutions.

Fig. 4 in Repatriation of knowledge about insects and types through the DORSA virtual museum (Digital Orthoptera Specimen Access)

Fig. 4: Documentation of DORSA specimen data on the internet. Left: species page in SYSTAX (red arrows mark scrolling down); top right: species page in OSF; middle right: image and sound file pages in SYSTAX; bottom right: distribution map from a map server (Canadian Biodiversity Information Facility). External links are marked by yellow arrows, internal links by green arrows.

Fig. 2 in Repatriation of knowledge about insects and types through the DORSA virtual museum (Digital Orthoptera Specimen Access)

Fig. 2: Species group names of Orthoptera worldwide (valid names and synonyms) and types specimens in German collections. The category holotype includes also syntypes, lectotypes and neotypes. [From Ingrisch et al. 2004a].

Fig. 1 in Repatriation of knowledge about insects and types through the DORSA virtual museum (Digital Orthoptera Specimen Access)

Fig. 1: Distribution of Orthoptera type specimens (including Para- and Lectotypes) housed in German Museum collections. Note the high number of types from Africa, South East Asia and Australia, collected in the last centuries. [From Riede 2003].

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Fig. 1 in Insects Associated with the European Mistletoe (Viscum album) in Western Ukraine: a Pilot Study

Fig. 1. Longitudinal section through the tissues of the host tree (Tilia cordata) and European mistletoe (Viscum album): A–A1 — section through intact tissues of mistletoe and host tree; B–B1, CC1 — section through infest- ed by xylophagous insects' tissues of mistletoe and host tree; D–D1 — section through infested by xylophagous insects' mistletoe tissue. A–D — sections in the original colors, A1–D1 — sections in the false colors. Labels: a (red) — xylophagous insects' galleries; b (pink) — wood of the host tree; c (orange) — bark and phloem of the host tree; d (yellow) — mistletoe wood; e (green) — bark and phloem of mistletoe; f (grey) — the external surface of plants.