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242 results for “Microfluidics”
The Evaluation of the Effect of Microfluidic Sperm Sorting Chip 'Labs-on-a-chip' on IVF Success in Male Factor
ClinicalTrials.gov study NCT03960229. IPD Sharing: UNDECIDED. Countries: 1. Publications: 3.
Development of Circulating Tumour Cell Molecular Diagnostics Using a Novel Microfluidic Device
ClinicalTrials.gov study NCT01193829. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Microfluidic Assessment of Clinical Outcomes in Preterm Newborns
ClinicalTrials.gov study NCT03291496. IPD Sharing: UNDECIDED. Countries: 1. Publications: 33.
Data from: Microfluidic PCR-based target enrichment: a case study in two rapid radiations of Commiphora (Burseraceae) from Madagascar
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Wide-range viscoelastic compression forces in microfluidics to probe cell-dependent nuclear structural and mechanobiological responses
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A novel approach to low-cost, rapid and simultaneous colorimetric detection of multiple analytes using 3D printed microfluidic channels
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Research data supporting "Void-free 3D bioprinting for in-situ endothelialization and microfluidic perfusion"
<p>Raw research data supporting the publication:</p> <p>Ouyang, Li. et al., 2019, Advanced Functional Materials. DOI: 10.1002/adfm.201908349</p>
Microfluidics system for the entrapment and detection of oocysts of Cryptosporidium
<p><em>Cryptosporidium</em> is an obligate intracellular protozoan parasite of great public health concern. Oocysts, the infectious form of <em>Cryptosporidium</em> species are ubiquitous in environmental sources such as soil and water all over the world and can stay viable up to six months. Several outbreaks of Cryptosporidiosis, a diarrheal illness in healthy and immunocompromised humans and in agriculturally important livestock species have been reported over the years owing to contaminated drinking water and recreational water supplies. Also, it has been a source of contaminants in salad vegetables and other raw leafy fruits and vegetables. A method for rapid monitoring of oocysts of <em>Cryptosporidium</em> species in water treatment plants and outside eateries will help to protect the public from incidences of Cryptosporidiosis. In the present study, Microfluidics device has been developed to entrap and detect the oocysts from environmental samples. The prepared device entraps and detects the oocysts at Micron level. The entire device is established on small chip sized area (2 cm x 2cm) utilizing less volume of samples and reagents. The device is cost effective and rapid in its action. Oocysts are detected by using FITC-labeled antibodies. The sensitivity of detection method is 35 %. Efficiency of trapping of positive oocysts is 0.078 % and oocysts from sample is 0.47% .</p>
On-site plant pathogen detection methods and introduction to microfluidics
<p>On June 24, 2020, IPANEMA organized the fourth Scientific online lecture (SOL4). The lesson was held by Dr. Cor Schoen from <a href="https://www.wur.nl/">Wageningen University & Research</a>, who brought us closer to different possible approaches for pathogen detection on the field.</p>
Microfluidics in Tissue Engineering
<p>On July 31, 2020, the fifth Scientific Online Lecture of the IPANEMA project was held. The lecture with the title “Microfluidics in tissue engineering” has been given by Prof. Dr Gordana Vunjak-Novaković, from <a href="https://www.columbia.edu/">Columbia University</a>. The lecture nicely described the basic principles of organs-on-a-chip, as well as possible microfluidics solutions in the IPANEMA project.</p>
Stepwise construction of dynamic microscale concentration gradients around hydrogel-encapsulated cells in a microfluidic perfusion culture device
<p><span>Inside living organisms, concentration gradients dynamically change as biological processes progress. Therefore, methods to construct dynamic microscale concentration gradients in a spatially controlled manner are needed to provide more realistic research environments. Here, we report a novel method for the construction of dynamic microscale concentration gradients in a stepwise manner around cells in micropatterned hydrogel. In our method, cells are encapsulated in a photodegradable hydrogel formed inside a microfluidic perfusion culture device, and perfusion microchannels are then fabricated in the hydrogel by micropatterned photodegradation. The cells in the micropatterned hydrogel can then be cultured by perfusing culture medium through the fabricated microchannels. By using this method, we demonstrate the simultaneous construction of two dynamic concentration gradients, which allowed us to expose the cells encapsulated in the hydrogel to a dynamic microenvironment.</span></p>
Data from: Facile fabrication of microfluidic surface-enhanced raman scattering devices via lift-up lithography
We describe a facile and low-cost approach for flexible integration surface-enhanced Raman scattering (SERS) substrate in microfluidic chips. Briefly, a SERS substrate was fabricated by electrostatic assembly of gold nanoparticles, and shaped into designed patterns by the subsequent lift-up soft lithography. The SERS micropattern could be further integrated within microfluidic channels conveniently. The resulting microfluidic SERS chip allows one to ultrasensitively in-situ SERS monitor from the transparent glass window. With its advantages in simplicity, functionality, and cost-effectivity, this method can be readily expanded in optical microfluidic fabrication for biochemical applications.
Long-Term Single Cell Analysis of S. pombe on a Microfluidic Microchemostat Array
<p>Although <em>Schyzosaccharomyces pombe</em> is one of the principal model organisms for studying the cell cycle, surprisingly few methods have characterized <em>S. pombe</em> growth on the single cell level, and no methods exist capable of analyzing thousands of cells and tens of thousands of cell division events. We developed an automated microfluidic platform permitting <em>S. pombe</em> to be grown on-chip for several days under defined and changeable conditions. We developed an image processing pipeline to extract and quantitate several physiological parameters including cell length, time to division, and elongation rate without requiring synchronization of the culture. Over a period of 50 hours our platform analyzed over 100000 cell division events and reconstructed single cell lineages up to 10 generations in length. We characterized cell lengths and division times in a temperature shift experiment in which cells were initially grown at 30°C and transitioned to 25°C. Although cell length was identical at both temperatures at steady-state, we observed transient changes in cell length if the temperature shift took place during a critical phase of the cell cycle. We further show that cells born with normal length do divide over a wide range of cell lengths and that cell length appears to be controlled in the second generation, were large newly born cells have a tendency to divide more rapidly and thus at a normalized cell size. The platform is thus applicable to measure fine-details in cell cycle dynamics, should be a useful tool to decipher the molecular mechanism underlying size homeostasis, and will be generally applicable to study processes on the single cell level that require large numbers of precision measurements and single cell lineages.</p>
A High-Throughput Microfluidic Platform for Mammalian Cell Transfection and Culturing
<p>Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent transfections at up to 99% efficiency. The chip can perform co-transfections, in which the number of cells expressing each protein and the average protein expression level can be precisely tuned as a function of input DNA concentration and synthetic gene circuits can be optimized on chip. We co-transfected four plasmids to test a histidine kinase signaling pathway and mapped the dose dependence of this network on the level of one of its constituents. The chip is readily integrated with high-content imaging, enabling the evaluation of cellular behavior and protein expression dynamics over time. These features make the transfection chip applicable to high-throughput mammalian protein and synthetic biology studies.</p>
In-operando visualization of redox flow battery in membrane-free microfluidic platform
<p>The video files are raw data of in-operando visualization of MFRFB that correspond to figure 2.C,E in manuscript.</p>
Massively parallel measurements of molecular interaction kinetics on a microfluidic platform
<p>Quantitative biology requires quantitative data. No high-throughput technologies exist capable of obtaining several hundred independent kinetic binding measurements in a single experiment. We present an integrated microfluidic device (k-MITOMI) for the simultaneous kinetic characterization of 768 biomolecular interactions. We applied k-MITOMI to the kinetic analysis of transcription factor (TF)—DNA interactions, measuring the detailed kinetic landscapes of the mouse TF Zif268, and the yeast TFs Tye7p, Yox1p, and Tbf1p. We demonstrated the integrated nature of k-MITOMI by expressing, purifying, and characterizing 27 additional yeast transcription factors in parallel on a single device. Overall, we obtained 2,388 association and dissociation curves of 223 unique molecular interactions with equilibrium dissociation constants ranging from 2 × 10<sup>-6</sup> M to 2 × 10<sup>-9</sup> M, and dissociation rate constants of approximately 6 s<sup>-1</sup> to 8.5 × 10<sup>-3</sup> s<sup>-1</sup>. Association rate constants were uniform across 3 TF families, ranging from 3.7 × 10<sup>6</sup> M<sup>-1</sup> s<sup>-1</sup> to 9.6 × 10<sup>7</sup> M<sup>-1</sup> s<sup>-1</sup>, and are well below the diffusion limit. We expect that k-MITOMI will contribute to our quantitative understanding of biological systems and accelerate the development and characterization of engineered systems.</p>
A Microfluidic Biodisplay
<p>Synthetically engineered cells are powerful and potentially useful biosensors, but it remains problematic to deploy such systems due to practical difficulties and biosafety concerns. To overcome these hurdles, we developed a microfluidic device that serves as an interface between an engineered cellular system, environment, and user. We created a biodisplay consisting of 768 individually programmable biopixels and demonstrated that it can perform multiplexed, continuous sampling. The biodisplay detected 10 μg/L sodium-arsenite in tap water using a research grade fluorescent microscope, and reported arsenic contamination down to 20 μg/L with an easy to interpret “skull and crossbones” symbol detectable with a low-cost USB microscope or by eye. The biodisplay was designed to prevent release of chemical or biological material to avoid environmental contamination. The microfluidic biodisplay thus provides a practical solution for the deployment and application of engineered cellular systems.</p>
Supplementary data: Uncured PDMS Inhibits Myosin In Vitro Motility in a Microfluidic Flow Cell
<p>This is the supplementary data of manucript 'Uncured PDMS Inhibits Myosin In Vitro Motility in a Microfluidic Flow Cell'.</p>
Clozapine Sensing Through Paper-based Microfluidic Sensors Directly Modified via Electrodeposition and Electropolymerization
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Custom microfluidic chip design enables cost-effective three-dimensional spatiotemporal transcriptomics with a wide field of view
<pre>Data analysis code and preprocessed data for MAGIC-seq.</pre>
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Allen Brain Atlas
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DANDI Archive for NWB datasets
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International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.