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435 results for “Sterilization”
Physico-chemical characterization of sterile Fe3O4 nanoparticles by XPS / HAXPES / SEM
<p>Here a dataset of XPS, HAXPES and SEM measurements for the physico-chemical characterization of nanoparticles is presented. The measurements are part of the H2020 project “NanoSolveIT”.</p> <p>Sterile Fe<sub>3</sub>O<sub>4</sub> nanoparticles were synthesized under aseptic conditions at Mintek, South Africa (EMR-Identifier in the project: ERM00000583).</p> <p>Prior to the measurements, the samples were prepared from the solution as drop cast on Si wafers. First SEM measurements were performed, followed by XPS and HAXPES measurements on the same samples. Here at first survey spectra were recorded followed by high resolution spectra. For XPS / HAXPES several Si-wafers were mounted together on one platen. The platen was left in the intro chamber of the instrument for several hours before the measurement started.</p> <p>Equipment:</p> <p>SEM images were acquired with a <em>Supra 40 </em>(Zeiss) SEM.</p> <p>For X-ray spectroscopy experiments, a combined XPS / HAXPES spectrometer (<em>Quantes </em>from ULVAC-PHI) was used, where XPS is measured at 1486.6 eV (monochromatic Al Kα source) and HAXPES at 5414.9 eV (monochromatic Cr Kα source). Here it is possible to perform the measurements at the exact same position.</p> <p>Data:</p> <p>For SEM, the data are given in .tif format. For XPS / HAXPES the raw data are given as .spe (PHI format) and .npl (VAMAS format) files. The measurement conditions are given in the data files.</p> <p>Naming of data:</p> <p>SEM: <em>sample_treatment (n)</em>, with n a consecutive number.</p> <p>XPS / HAXPES: For the .spe and for the corresponding .npl files <em>Pn.m.o.sample_treatment, </em>with Pn: platen-number, m: spectrum number (order of the measurements); o: point-number, of the position on the sample, sample_treatment with "p" for pristine.</p> <p> </p> <p>The authors thank Thorid Lange, who performed the SEM measurements.</p>
Physico-chemical characterization of sterile ZnO nanoparticles by XPS / HAXPES / SEM
<p>Here a dataset of XPS, HAXPES and SEM measurements for the physico-chemical characterization of nanoparticles is presented. The measurements are part of the H2020 project “NanoSolveIT”.</p> <p>Sterile ZnO<sub> </sub>nanoparticles were synthesized under aseptic conditions at Mintek, South Africa (EMR-Identifier in the project: ERM00000584).</p> <p>Prior to the measurements, the samples were prepared from the solution as drop cast on Si wafers. First SEM measurements were performed, followed by XPS and HAXPES measurements on the same samples. Here at first survey spectra were recorded followed by high resolution spectra. For XPS / HAXPES several Si-wafers were mounted together on one platen. The platen was left in the intro chamber of the instrument for several hours before the measurement started.</p> <p>Equipment:</p> <p>SEM images were acquired with a <em>Supra 40 </em>(Zeiss) SEM.</p> <p>For X-ray spectroscopy experiments, a combined XPS / HAXPES spectrometer (<em>Quantes </em>from ULVAC-PHI) was used, where XPS is measured at 1486.6 eV (monochromatic Al Kα source) and HAXPES at 5414.9 eV (monochromatic Cr Kα source). Here it is possible to perform the measurements at the exact same position.</p> <p>Data:</p> <p>For SEM, the data are given in .tif format. For XPS / HAXPES the raw data are given as .spe (PHI format) and .npl (VAMAS format) files. The measurement conditions are given in the data files.</p> <p>Naming of data:</p> <p>SEM: <em>sample_treatment (n)</em>, with n a consecutive number.</p> <p>XPS / HAXPES: For the .spe and for the corresponding .npl files <em>Pn.m.o.sample_treatment, </em>with Pn: platen-number, m: spectrum number (order of the measurements); o: point-number, of the position on the sample, sample_treatment with "p" for pristine.</p> <p> </p> <p>The authors thank Thorid Lange, who performed the SEM measurements.</p>
Physico-chemical characterization of sterile citrated stabilized Au nanoparticles by XPS / HAXPES / SEM
<p>Here a dataset of XPS, HAXPES and SEM measurements for the physico-chemical characterization of nanoparticles is presented. The measurements are part of the H2020 project “NanoSolveIT”.</p> <p>Sterile citrated stabilized Au nanoparticles with a diameter of approx. 30 nm were synthesized under aseptic conditions at Mintek, South Africa (EMR-Identifier in the project: ERM00000582).</p> <p>Prior to the measurements, the samples were prepared from the solution as drop cast on Si wafers. First SEM measurements were performed, followed by XPS and HAXPES measurements on the same samples. Here at first survey spectra were recorded followed by high resolution spectra. For XPS / HAXPES several Si-wafers were mounted together on one platen. The platen was left in the intro chamber of the instrument for several hours before the measurement started.</p> <p>Equipment:</p> <p>SEM images were acquired with a <em>Supra 40 </em>(Zeiss) SEM.</p> <p>For X-ray spectroscopy experiments, a combined XPS / HAXPES spectrometer (<em>Quantes </em>from ULVAC-PHI) was used, where XPS is measured at 1486.6 eV (monochromatic Al Kα source) and HAXPES at 5414.9 eV (monochromatic Cr Kα source). Here it is possible to perform the measurements at the exact same position.</p> <p>Data:</p> <p>For SEM, the data are given in .tif format. For XPS / HAXPES the raw data are given as .spe (PHI format) and .npl (VAMAS format) files. The measurement conditions are given in the data files.</p> <p>Naming of data:</p> <p>SEM: <em>sample_treatment (n)</em>, with n a consecutive number.</p> <p>XPS / HAXPES: For the .spe and for the corresponding .npl files <em>Pn.m.o.sample_treatment, </em>with Pn: platen-number, m: spectrum number (order of the measurements); o: point-number, of the position on the sample, sample_treatment with "p" for pristine.</p> <p> </p> <p>The authors thank Thorid Lange, who performed the SEM measurements.</p>
Developmental heat stress interrupts spermatogenesis inducing early male sterility in Drosophila melanogaster
<p><span>Thermal stress leads to fertility reduction, can cause temporal sterility and thus results in fitness loss with severe ecological and evolutionary consequences, e.g., threatening species persistence already at sub-lethal temperatures. For males we here tested which developmental stage is particularly sensitive to heat stress in the model species <em>Drosophila</em> <em>melanogaster</em>. As developmental stages characterize the different steps of sperm development, we could narrow down which particular processes are heat sensitive. We studied early male reproductive ability and, by following recovery dynamics after a move to benign temperatures, we investigated the mechanisms behind a subsequent gain of fertility. We found strong support to suggest that the last steps of spermatogenesis are particularly sensitive to heat stress, as processes occurring during the pupal stage were mostly interrupted, delaying both sperm production and sperm maturation. Moreover, further measurements in the testes and for proxies of sperm availability indicating the onset of adult reproductive capacity matched the expected heat-induced delay in completing spermatogenesis. We discuss these results within the context of how heat stress affects reproductive organ function and the consequences for male reproductive potential.</span></p>
Cytoplasmic male sterility and abortive seed traits generated through mitochondrial genome editing coupled with allotopic expression of atp1 in tobacco
<p>Genome editing tools have recently been developed that are capable of generating deletions and/or rearrangements in plant mitochondrial genomes. We utilized custom-designed meganuclease constructs targeting the essential mitochondrial gene <em>atp1</em> in tobacco plants carrying a version of atp1 that had been repurposed to function as a nuclear gene. Leaf extracts enriched in mitochondria from three independent genome editing events were evaluated by PacBio sequencing and analysis. Contigs that display sequence homology to the reference mitochondrial genome BA000042 are deposited here. Some of the contigs correspond to the tobacco mitochondrial genome as expected, while others corresponded to sequences from the nucleus where the ancient transfer of portions of the mitochondrial genome became incorporated into the nuclear genome. Still other contigs correspond to the chloroplast genome, or pieces of the chloroplast genome anciently transferred to the nucleus, as small fragments of the chloroplast genome are interspersed in plant mitochondrial genomes. Analysis of contigs of genuine mitochondrial origin demonstrated that the double-strand breaks imposed by the genome editing enzymes resulted in the deletion of <em>atp1</em> and neighboring sequences, along with rearrangements in overall genome structure.</p>
Tissue Adhesive vs. Sterile Strips After Cesarean Delivery
ClinicalTrials.gov study NCT02838017. IPD Sharing: YES. Countries: 1. Publications: 8.
Intracutaneous Sterile Water Injections
ClinicalTrials.gov study NCT01513447. IPD Sharing: Not stated. Countries: 1. Publications: 9.
Intrauterine Lidocaine Infusion for Essure Sterilization Procedures
ClinicalTrials.gov study NCT00613834. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Phase II Study of SHJ002 Sterile Ophthalmic Solution Compared With Vehicle in Participants With Dry Eye Disease
ClinicalTrials.gov study NCT05486728. IPD Sharing: NO. Countries: 3. Publications: 0.
Data from: De novo transcriptome characterization of a sterilizing trematode parasite (Microphallus sp.) from two species of New Zealand snails
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Data from: Genomic islands of differentiation in two songbird species reveal candidate genes for hybrid female sterility
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The growth traits of Trifolium pratense under unsterilized and sterilized cow dung
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The genetic basis of cytoplasmic male sterility and fertility restoration in wheat
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Data from: Genotype and male sterility phenotype data for An. coluzzii x An. quadriannulatus backcross
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Data from: Natural variation in the zinc-finger-encoding exon of Prdm9 affects hybrid sterility phenotypes in mice
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Effects of consumer surface sterilization on diet DNA metabarcoding data of terrestrial invertebrates in natural environments and feeding trials
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Developmental heat stress interrupts spermatogenesis inducing early male sterility in Drosophila melanogaster
Open the record for dataset details and reuse information.
Cytoplasmic male sterility and abortive seed traits generated through mitochondrial genome editing coupled with allotopic expression of atp1 in tobacco
Open the record for dataset details and reuse information.
Sex-specific sterility caused by extreme temperatures is likely to create cryptic changes to the operational sex ratio in Drosophila virilis
<p>Climate change is increasing the frequency and severity of short-term heat shocks that threaten the persistence of natural populations. The effect of thermal stress on natural selection is a common topic of debate, but high temperatures can also influence sexual selection. Typically, males and females of a species can survive at similar extreme temperatures, but males have been shown to lose fertility at lower temperatures than females. Here, we examine how a brief exposure of pupae to high temperatures in the fruit fly Drosophila virilis affects adult fertility in both males and females. We find strong sexual dimorphism in temperature-induced sterility. This has the potential to quickly and unpredictably create populations composed of mostly sterile males and fertile females, resulting in changes to the operational sex ratio (OSR). These disruptions are likely to be cryptic and difficult to measure in the wild, especially considering that males can eventually recover fertility and that sterile males of some species can still copulate. Changes to the OSR in this way are likely to influence sexual selection by favouring females that can discriminate between fertile and sterilised males, and possibly leading to female-female conflict over a limited pool of fertile males. Further research on how cryptic disruptions to the OSR affect sexual selection dynamics is critical for understanding the impact of environmental change on biodiversity.</p>
Mating and survival of sterile Drosophila suzukii, in presence and absence of food
<p><em>Drosophila suzukii </em>(Matsumura) (Diptera: Drosophilidae) is a widely distributed pest species of soft-skinned fruits. Recent studies suggest the use of Sterile Insect Technique (SIT) as a control method for this species, however, many are the factors that can impact effectiveness of a SIT program, including environmental condition. Thus, we aimed to verify the influence of temperature and relative humidity on mating and survival of fertile and sterile <em>D. suzukii, </em>when insects were food provided or deprived. Highest mating rates were obtained when food provided flies (either fertile or sterile) were exposed to 25 ºC or 81-100% relative humidity, while extreme temperatures (10 and 35 ºC) and low humidity (below 60%) impaired mating. Overall, mating rate among food deprived flies was low in all temperatures and humidity levels tested, but fertile insects were more prone to mate when compared to sterile flies. Survival was negatively influenced by high temperatures, low relative humidity and food deprivation. The information present in this study is useful to be considered for transport and release of sterile <em>D. suzukii</em>.</p>
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