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240 results for “Tissue imaging”
Intravoxel incoherent motion model of diffusion weighted imaging and diffusion kurtosis imaging in differentiating of local colorectal cancer recurrence from scar/fibrosis tissue by multivariate logistic regression analysis
<p>We uploaded mean of diffusion coefficient (MD) and mean of diffusional Kurtosis values of 56 patients related to the manuscript: Fusco, Roberta, Vincenza Granata, Mario Sansone, Robert Grimm, Paolo Delrio, Daniela Rega, Fabiana Tatangelo, Antonio Avallone, Nicola Raiano, Giuseppe Totaro, Vincenzo Cerciello, Biagio Pecori, and Antonella Petrillo. 2020. "Intravoxel Incoherent Motion Model of Diffusion Weighted Imaging and Diffusion Kurtosis Imaging in Differentiating of Local Colorectal Cancer Recurrence from Scar/Fibrosis Tissue by Multivariate Logistic Regression Analysis" Applied Sciences 10, no. 23: 8609. https://doi.org/10.3390/app10238609</p>
Imaging Mass Cytometry of human normal colon mucosa (CLN1-6) from: A SIMPLI (Single-cell Identification from MultiPLexed Images) approach for spatially resolved tissue phenotyping at single-cell resolution.
<p>Four µm-thick sections were cut from each block of samples CLN1-CLN6 with a microtome and used for staining with a panel of 26 antibodies targeting the main immune, stromal and epithelial cell populations of the gastrointestinal tract (Supplementary Table 2). The optimal dilution of each antibody in the panel was identified by staining and ablating FFPE appendix sections. The resulting images were reviewed by a mucosal immunologist (J.S.) and the dilution giving the best signal to background ratio was selected for each antibody (Supplementary Table 2). To perform the staining for IMC, slides were dewaxed after a one-hour incubation at 60°C, rehydrated and heat-induced antigen retrieval was performed with a pressure cooker in Antigen Retrieval Reagent-Basic (R&D Systems). Slides were incubated in a 10% BSA (Sigma), 0.1% Tween (Sigma), and 2% Kiovig (Shire Pharmaceuticals) Superblock Blocking Buffer (Thermo Fisher) blocking solution at room temperature for two hours. Each antibody was added to a primary antibody mix at the selected concentration in blocking solution and incubated overnight at 4°C. After two washes in PBS and PBS-0.1% Tween, the slides were treated with the DNA intercalator Cell-ID™ Intercalator-Ir (Fluidigm) (containing the two iridium isotopes 191Ir and 193Ir) 1.25 mM in a PBS solution. After a 30-minute incubation, the slides were washed once in PBS and once in MilliQ water and air-dried. The stained slides were then loaded in the Hyperion Imaging System (Fluidigm) imaging module to obtain light-contrast high resolution images of approximately four mm<sup>2</sup>. These images were used to select the ROI in each slide. For CLN1-CLN6, 1 mm<sup>2 </sup>ROIs were selected to contain the full thickness of the colon mucosa, with epithelial crypts in longitudinal orientation. ROIs were ablated at a o µm/pixel resolution and 200 Hz frequency.</p> <p>Twenty-eight images from 26 antibodies (Supplementary Table 2) and two DNA intercalators were obtained from the raw .txt files of the ablated regions in CLN1-CLN6 using the data extraction process.</p>
Example sdt raw FLIM images of NAD(P)H autofluorescence in Drosophila melanogaster tissues
<p>Raw image files of fluorescence lifetime imaging microscopy (FLIM) of NAD(P)H autofluorescence in unstained living <em>Drosophila melanogaster</em> tissues for image analysis. See <a href="https://www.nature.com/articles/s41598-019-56067-w">publication</a> for technical details. Dataset is derived by reduction of original <a href="http://dx.doi.org/10.25532/OPARA-37">dataset</a> to a single channel and selection of individual images. A single 2 channel sdt FLIM image is included also in which the first channel corresponds to the proper NAD(P)H detection range.</p>
A closer look at high-energy X-ray-induced bubble formation during soft tissue imaging
<p>Improving the scalability of tissue imaging throughput with bright, coherent X-rays requires identifying and mitigating artifacts resulting from the interactions between X-rays and matter. At synchrotron sources, long-term imaging of soft tissues in solution can result in gas bubble formation or cavitation, which dramatically compromises image quality and integrity of the samples. By combining in-line phase-contrast cineradiography with <em>operando</em> gas chromatography, we were able to track the onset and evolution of high-energy X-ray-induced gas bubbles in ethanol-embedded soft tissue samples for tens of minutes (2 to 3 times the typical scan times). We demonstrate quantitatively that vacuum degassing of the sample during preparation can significantly delay bubble formation, offering up to a twofold improvement in dose tolerance, depending on the tissue type. However, once nucleated, bubble growth is faster in degassed than undegassed samples, indicating their distinct metastable states at bubble onset. Gas chromatography analysis shows increased solvent vaporization concurrent with bubble formation, yet the quantities of dissolved gases remain unchanged. Coupling features extracted from the radiographs with computational analysis of bubble characteristics, we uncover dose-controlled kinetics and nucleation site-specific growth. These hallmark signatures provide quantitative constraints on the driving mechanisms of bubble formation and growth. Overall, the observations highlight bubble formation as a critical, yet often overlooked hurdle in upscaling X-ray imaging for biological tissues and soft materials and we offer an empirical foundation for their understanding and imaging protocol optimization. More importantly, our approaches establish a top-down scheme to decipher the complex, multiscale radiation-matter interactions in these applications.</p>
Supplementary movies and datasets of the paper: Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP
<p>Imaging data supporting the paper: </p> <p>Precise targeting for 3D cryo-correlative light and electron microscopy volume imaging of tissues using a FinderTOP, containing raw and processed data from fluorescent and electron microscopy.</p> <p> </p>
Robust phenotyping of highly multiplexed tissue imaging data using pixel-level clustering (data)
<p>MIBI-TOF data for lymph node dataset reported in Liu et al., Robust phenotyping of highly multiplexed tissue imaging data using pixel-level clustering</p> <p>1. mibi_single_channel_tifs.zip: Single-channel MIBI-TOF images</p> <p>Folders are labeled according to the field-of-view (FOV) number. Each folder contains single-channel TIFFs for each marker in the panel. Images are 1024x1024 pixels, 500 um. See paper for details.</p> <p>2. segmentation.zip: Segmentation output of MIBI-TOF images</p> <p>Cell segmentation was performed using Mesmer (Greenwald NF, Nature Biotechnology 2021). Output of Mesmer that delineates the single cells in each of the images is included.</p> <p>3. source_data.zip: Source data files for figures</p> <ul> <li>pixel_ccs_allpreprocessing.csv: Cluster consistency score (CCS) for all pixels using all preprocessing steps, related to Fig. 2d-f, Supp. Fig. 4,5,9,10</li> <li>pixel_ccs_nopixelnorm.csv: CCS for all pixels where pixel normalization was left out, related to Fig. 2f, Supp. Fig. 6</li> <li>pixel_ccs_nochannelnorm.csv: CCS for all pixels where channel normalization was left out, related to Fig. 2f, Supp. Fig. 8</li> <li>pixel_ccs_passes1.csv: CCS for all pixels where 1 pass was used for SOM training, related to Supp. Fig. 10g</li> <li>pixel_ccs_passes100.csv: CCS for all pixels where 100 passes were used for SOM training, related to Supp. Fig. 10g</li> <li>pixel_ccs_sigma0.csv: CCS for all pixels where a Gaussian blur sigma of 0 was used for preprocessing, related to Supp. Fig. 5d</li> <li>pixel_ccs_sigma1.csv: CCS for all pixels where a Gaussian blur sigma of 1 was used for preprocessing, related to Supp. Fig. 5d</li> <li>pixel_ccs_sigma3.csv: CCS for all pixels where a Gaussian blur sigma of 3 was used for preprocessing, related to Supp. Fig. 5d</li> <li>pixel_ccs_sigma0_reps100.csv: CCS for all pixels where a Gaussian blur sigma of 0 was used for preprocessing and 100 replicates were used for CCS calculation, related to Supp. Fig. 5e</li> <li>pixel_ccs_sigma1_reps100.csv: CCS for all pixels where a Gaussian blur sigma of 1 was used for preprocessing and 100 replicates were used for CCS calculation, related to Supp. Fig. 5e</li> <li>pixel_ccs_sigma2_reps100.csv: CCS for all pixels where a Gaussian blur sigma of 2 was used for preprocessing and 100 replicates were used for CCS calculation, related to Supp. Fig. 5e</li> <li>pixel_ccs_sigma3_reps100.csv: CCS for all pixels where a Gaussian blur sigma of 3 was used for preprocessing and 100 replicates were used for CCS calculation, related to Supp. Fig. 5e</li> <li>pixel_ccs_nodes15.csv: CCS for all pixels where 15 nodes were used for SOM training, related to Supp. Fig. 9e</li> <li>pixel_ccs_threshold80.csv: CCS for all pixels where a threshold of 80% was used for CCS calculation, related to Supp. Fig. 4b</li> <li>pixel_ccs_threshold98.csv: CCS for all pixels where a threshold of 98% was used for CCS calculation, related to Supp. Fig. 4b</li> <li>pixel_info_comparison_table.csv: Number of pixels that were assigned to a cluster outside of cell segmentation masks, related to Fig. 3d</li> <li>single_cell_pixel_composition_table.csv: Pixel composition information for each single cell, related to Fig. 5, Supp. Fig 16</li> <li>single_cell_integrated_expression_table.csv: Integrated expression per cell, output by Mesmer, related to Fig. 5, Supp. Fig. 16</li> <li>cell_silhouette_scores.csv: Silhouette scores for comparing integrated expression and pixel composition, related to Fig. 5d</li> <li>cell_silhouette_scores_undefinedremoved.csv: Silhouette scores for comparing integrated expression and pixel composition where undefined cells were removed, related to Fig. 16g</li> <li>cell_silhouette_scores_preprocessed.csv: Silhouette scores for comparing integrated expression and pixel composition where pixels were preprocessed before integrating expression, related to Fig. 17d</li> <li>cell_silhouette_scores_ilastik_cellprofiler.csv: Silhouette scores for comparing integrated expression and pixel composition where segmentation masks were obtained using Ilastik/CellProfiler, related to Fig. 18b</li> <li>cell_ccs_pixel_composition.csv: CCS for all cells using pixel composition for clustering, related to Supp. Fig. 16e, 17c</li> <li>cell_ccs_integrated_expression.csv: CCS for all cells using integrated expression for clustering, related to Supp. Fig 16e-f</li> <li>cell_ccs_integrated_expression_preprocessed.csv: CCS for all cells using integrated expression for clustering where data was preprocessed before integrating, related to Supp. Fig 17c</li> <li>cytof_ccs.csv: CCS of the CyTOF dataset used as a benchmark, related to Supp. Fig. 4c,d</li> <li>scrnaseq_ccs.csv: CCS of the scRNA-seq dataset used as a benchmark, related to Supp. Fig. 4c,e</li> <li>pixel_phenotype_maps: TIFFs where pixel value corresponds to pixel cluster number as reported in the paper</li> <li>cell_phenotype_maps: TIFFs where pixel value corresponds to cell cluster number as reported in the paper</li> <li>runtime_analysis_pixel.csv: Runtime analysis of pixel clustering in Pixie, related to Supp. Fig. 22a</li> <li>runtime_analysis_cell.csv: Runtime analysis of cell clustering in Pixie, related to Supp. Fig. 22b</li> <li>runtime_clustering_algorithm.csv: Runtime analysis of different clustering algorithms, related to Supp. Fig. 22c</li> </ul>
Data for: Contributions of deep learning to automated numerical modelling of the interaction of electric fields and cartilage tissue based on 3D images
<p>Replication data for: Contributions of deep learning to automated numerical modelling of the interaction of electric fields and cartilage tissue based on 3D images</p> <p> </p> <p> </p>
DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging
<p>Tiff images, single cell data, and cell masks for the publication "DNA-barcoded signal amplification for imaging mass cytometry enables sensitive and highly multiplexed tissue imaging". The code used to produce the results of this study is available at https://github.com/BodenmillerGroup/SABER-IMC_publication</p>
FLuoresence Image Guided Surgery With A VEGF-targeted Tracer in Soft-tissue Sarcomas in Humans Approach With Bevacizumab-IRDye 800CW
ClinicalTrials.gov study NCT03913806. IPD Sharing: UNDECIDED. Countries: 1. Publications: 1.
A Study of Liposomal Doxorubicin in Women With Breast Cancer Exploiting Tissue Doppler Imaging
ClinicalTrials.gov study NCT00531973. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Multi-elemental Imaging of Lung Tissues With LIBS (Laser-induced Breakdown Spectroscopy)
ClinicalTrials.gov study NCT03901196. IPD Sharing: NO. Countries: 1. Publications: 1.
Prophylactic Mastectomy: Prospective Evaluation of the Correlation Between Skin Flap Thickness, Residual Glandular Tissue and Skin Necrosis by Imaging and Clinical Examination
ClinicalTrials.gov study NCT05162677. IPD Sharing: UNDECIDED. Countries: 1. Publications: 2.
Malignant Myoepithelioma of Bone and Soft Tissues: Diagnostic Imaging and Histology in Relation to Prognosis
ClinicalTrials.gov study NCT06244420. IPD Sharing: NO. Countries: 1. Publications: 2.
CHinese Acute Tissue-Based Imaging Selection for Lysis In Stroke -Tenecteplase
ClinicalTrials.gov study NCT04086147. IPD Sharing: Not stated. Countries: 1. Publications: 3.
The Norepinephrine Transporter: A Novel Target for Imaging Brown Adipose Tissue
ClinicalTrials.gov study NCT02038595. IPD Sharing: Not stated. Countries: 1. Publications: 1.
Functional Imaging of Tumor and Normal Tissue
ClinicalTrials.gov study NCT00933114. IPD Sharing: Not stated. Countries: 1. Publications: 2.
Imaging Ahmed Glaucoma Tubes With a Pericardial Graft and Tissue Glue or Partial-Thickness Scleral Flap and Sutures
ClinicalTrials.gov study NCT00453024. IPD Sharing: Not stated. Countries: 1. Publications: 2.
A PET Imaging Agent to Assess the Level of Tumor Tissue-infiltrating CD8 + T Cells in Patients With Solid Tumors
ClinicalTrials.gov study NCT05126927. IPD Sharing: NO. Countries: 1. Publications: 2.
Evaluation of Virtual Touch Tissue Imaging Quantification (VTIQ - 2D-SWE) in the Assessment of BI-RADS® 3 and 4 Lesions
ClinicalTrials.gov study NCT02638935. IPD Sharing: Not stated. Countries: 6. Publications: 13.
CHinese Acute Tissue-Based Imaging Selection for Lysis In Stroke -Tenecteplase II
ClinicalTrials.gov study NCT04516993. IPD Sharing: Not stated. Countries: 1. Publications: 3.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.