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153 results for “cuticle”
Figure 5 from: Mrak P, Žnidaršič N, Tušek-Žnidarič M, Klepal W, Gruber D, Strus J (2012) Egg envelopes and cuticle renewal in Porcellio embryos and marsupial mancas. ZooKeys 176: 55-72. https://doi.org/10.3897/zookeys.176.2418
Figure 5 - Cuticle structure and renewal in Porcellio scaber marsupial mancas. A The early-stage marsupial manca, immediately after hatching B The mid-stage marsupial manca C The late-stage marsupial manca, just prior to release from the marsupium D–F Semithin sections of the manca peripheral region in the early-stage marsupial manca D in the mid-stage marsupial manca E and in the late-stage marsupial manca F. Cuticle (c), overlying the hypodermis (hd), becomes progressively more similar to adult cuticle. G–K TEM micrographs of exoskeletal cuticle in the early-stage marsupial manca G, J in the mid-stage marsupial manca H and in the late-stage marsupial manca I, K Three main layers are distinguished: epicuticle (ep), exocuticle (ex) and endocuticle (en). The micrographs show morphological characteristics of cuticle renewal: detachment of the old cuticle (oc) from the hypodermis, ecdysal space (*) between the detached cuticle and the newly forming cuticle (nc) and partial degradation of the old cuticle H, I protrusions with electron dense tips (white arrows) on apical surfaces of hypodermal cells G, I, J. The new cuticle consists of two layers, external electron dense epicuticle and internal electron lucent procuticle H, I, K. Helicoidal chitin-protein fibers arrangement is discernible in some regions of late-stage marsupial manca K. Bars: A–C 500 µm; D–F 10 µm; G–I 1 µm; J, K 200 nm.
Figure 3 from: Mrak P, Žnidaršič N, Tušek-Žnidarič M, Klepal W, Gruber D, Strus J (2012) Egg envelopes and cuticle renewal in Porcellio embryos and marsupial mancas. ZooKeys 176: 55-72. https://doi.org/10.3897/zookeys.176.2418
Figure 3 - Structure of vitelline membrane (vm), covering Porcellio scaber A–D and Porcellio dilatatus E–G late-stage embryo. A Ventrally bent late-stage embryo, yolk is completely enclosed into the midgut glands (mg). B Semithin section of the embryo peripheral region. Vitelline membrane is slightly detached from the hypodermis (hd). C, D TEM micrographs of the vitelline membrane in osmicated specimen C and in non-osmicated specimen D Main proximal homogenous layer (*), thin middle electron dense layer (white arrow) and superficial corrugated lucent layer (black arrow). Hypodermis is covered with an extracellular matrix (ECM). E SEM micrograph of the late-stage embryo surrounded by vitelline membrane. F, G SEM micrographs of the late-stage embryo surface area. The vitelline membrane is artificially slit and fibers (arrows) between the outer embryo surface, covered with an extracellular matrix (s), and the vitelline membrane are exposed. Bars: A 500 µm; B, F 10 µm; C, D 200 nm; E 200 µm; G 5 µm.
Figure 3 from: Ziegler A, Seidl B (2012) Electron microscopic and preparative methods for the analysis of isopod cuticle. ZooKeys 176: 73-85. https://doi.org/10.3897/zookeys.176.2294
Figure 3 - FE-SEM micrographs of polished sagittal plane through bulk tergite samples etched at pH 6.5 A, B, C and 8.0 D, E. A Fibres in the distal exocuticle (dex) and the isle-like structure of the proximal exocuticle (pex) caused by large pore canals (pc). Fibrils or fibres (arrowheads) in the pore canals are well visible. en, endocuticle. B, C Side views of tricorn sensilla. The epicuticular unmineralised material forming the sensilla is well distinguishable from the mineralised exo- and endocuticle. ep, epicuticle; sc, epicuticular scale. D, E Mild etching reveals regions containing mineral of different solubility. Mineral within the endocuticle appears etched whereas most regions within the exocuticle (ex) remain unaltered. Note etching within pore canals of the exocuticle.
Figure 7 from: Mrak P, Žnidaršič N, Tušek-Žnidarič M, Klepal W, Gruber D, Strus J (2012) Egg envelopes and cuticle renewal in Porcellio embryos and marsupial mancas. ZooKeys 176: 55-72. https://doi.org/10.3897/zookeys.176.2418
Figure 7 - Schematic representation of different protective envelopes, coating Porcellio scaber embryos and marsupial mancas during development (lasting 35 days), namely egg envelopes (chorion and vitelline membrane) and exoskeletal cuticle. During growth of embryos and mancas egg envelopes are shed and cuticle is renewed. ch – chorion; vm – vitelline membrane; ECM – extracellular matrix; epi – epicuticle; exo – exocuticle; endo – endocuticle; nc – newly assembling cuticle.
Figure 1 from: Mrak P, Žnidaršič N, Tušek-Žnidarič M, Klepal W, Gruber D, Strus J (2012) Egg envelopes and cuticle renewal in Porcellio embryos and marsupial mancas. ZooKeys 176: 55-72. https://doi.org/10.3897/zookeys.176.2418
Figure 1 - Structure of distal chorion (ch) and proximal vitelline membrane (vm), covering Porcellio scaber A, B, D, F and Porcellio dilatatus C, E early-stage embryo. A The early-stage embryo with large amount of yolk (y) and no visible limb buds. B Semithin section of the embryo peripheral region. Chorion is separated from the embryo surface. The vitelline membrane is closely apposed to the embryo surface. C SEM micrograph of the early-stage embryo. The outer egg envelope, chorion, is visible. D TEM micrograph of one-layered chorion, including electron lucent "lacunae" (white arrow). There is a layer of artificially spilt yolk underneath the chorion. E SEM micrograph of the early-stage embryo. Chorion is artificially removed and the inner egg envelope, vitelline membrane, is exposed. F TEM micrograph of vitelline membrane, composed of three layers: main proximal homogenous layer (*), thin middle electron dense layer (white arrow) and superficial corrugated lucent layer (black arrow). Bars: A, C, E 200 µm; B 10 µm; D 0.5 µm; F 200 nm.
Figure 1 from: Ziegler A, Seidl B (2012) Electron microscopic and preparative methods for the analysis of isopod cuticle. ZooKeys 176: 73-85. https://doi.org/10.3897/zookeys.176.2294
Figure 1 - TEM A, G, H, I and STEM B, C, D, E, F micrographs of decalcified and EPON embedded tergites of Porcellio scaber. A Sagittal overview showing epicuticle (ep), exocuticle (ex), endocuticle (en) and membranous layer (ml), ec, epithelial cell; n, nucleus; sc, epicuticular scale. B Proximal exocuticle. Dense network of pore canals (pc) containing fibrils or fibres following the direction of the pore canal. C, D Fibres of the distal exocuticle (dex) consisting of approximately 3 nm thick unstained chitin crystallites (arrowheads) surrounded by densely stained proteins. E, F In the endocuticle single fibrils form the twisted plywood structure. The pore canals contain vertical fibrils or fibres. G, H, I Section through a cuticular thickening. The increase in cuticle thickness is brought about by an increase of the stacking height in the distal exocuticle only. H, I Details of G confirm the typical structure of fibres within the distal exocuticle.
Figure 2 from: Ziegler A, Seidl B (2012) Electron microscopic and preparative methods for the analysis of isopod cuticle. ZooKeys 176: 73-85. https://doi.org/10.3897/zookeys.176.2294
Figure 2 - STEM micrographs of non-decalcified tergites of Porcellio scaber. A Overview showing the mineralised exocuticle (ex) and endocuticle en and the unmineralised membranous layer (ml). The pore canals (arrows) are mineralised. B, C Exocuticle and epicuticle (ep). Approximately 25 nm thick fibres in the distal exocuticle (dex) and approximately 6 nm thick fibrils in the proximal exocuticle (pex). Pore canals arrows contain mineral. The inner epicuticle (iep) appears partly mineralised, and the outer epicuticle (oep) unmineralised, except epicuticular pore canals (epc). D, E Endocuticle with mineralised pore canal (arrow). Approximately 6 nm thick chitin-protein fibrils (black arrowheads) individually surrounded by mineral forming a twisted plywood structure. F, G Single mineral rods (white arrowheads) at the border between membranous layer and endocuticle.
Figure 7 from: Giurginca A, Šustr V, Tajovský K, Giurginca M, Matei I (2015) Spectroscopic parameters of the cuticle and ethanol extracts of the fluorescent cave isopod Mesoniscus graniger (Isopoda, Oniscidea). In: Taiti S, Hornung E, Štrus J, Bouchon D (Eds) Trends in Terrestrial Isopod Biology. ZooKeys 515: 111–125. https://doi.org/10.3897/zookeys.515.9395
Figure 7 - FP spectra of the sample of solid phase of Mesoniscus graniger dragani (λex = 380 nm) (Int.- intensity of the peak).
Figure 8 from: Giurginca A, Šustr V, Tajovský K, Giurginca M, Matei I (2015) Spectroscopic parameters of the cuticle and ethanol extracts of the fluorescent cave isopod Mesoniscus graniger (Isopoda, Oniscidea). In: Taiti S, Hornung E, Štrus J, Bouchon D (Eds) Trends in Terrestrial Isopod Biology. ZooKeys 515: 111–125. https://doi.org/10.3897/zookeys.515.9395
Figure 8 - FP spectra of the samples of ethanol extracts of Mesoniscus graniger graniger (G) and Mesoniscus graniger dragani (D) samples (λex = 380 nm) (F (a.u.) = fluorescence arbitrary units).
Figure 5 from: Giurginca A, Šustr V, Tajovský K, Giurginca M, Matei I (2015) Spectroscopic parameters of the cuticle and ethanol extracts of the fluorescent cave isopod Mesoniscus graniger (Isopoda, Oniscidea). In: Taiti S, Hornung E, Štrus J, Bouchon D (Eds) Trends in Terrestrial Isopod Biology. ZooKeys 515: 111–125. https://doi.org/10.3897/zookeys.515.9395
Figure 5 - UV-VIS-NIR spectra of the ethanol extracts of Mesoniscus graniger graniger (G) and Mesoniscus graniger dragani (D) (Abs = Absorbance units).
Figure 4 from: Giurginca A, Šustr V, Tajovský K, Giurginca M, Matei I (2015) Spectroscopic parameters of the cuticle and ethanol extracts of the fluorescent cave isopod Mesoniscus graniger (Isopoda, Oniscidea). In: Taiti S, Hornung E, Štrus J, Bouchon D (Eds) Trends in Terrestrial Isopod Biology. ZooKeys 515: 111–125. https://doi.org/10.3897/zookeys.515.9395
Figure 4 - UV-VIS-NIR spectra of the sample of solid phase of Mesoniscus graniger dragani (% R = percent reflectance).
Figure 2 from: Giurginca A, Šustr V, Tajovský K, Giurginca M, Matei I (2015) Spectroscopic parameters of the cuticle and ethanol extracts of the fluorescent cave isopod Mesoniscus graniger (Isopoda, Oniscidea). In: Taiti S, Hornung E, Štrus J, Bouchon D (Eds) Trends in Terrestrial Isopod Biology. ZooKeys 515: 111–125. https://doi.org/10.3897/zookeys.515.9395
Figure 2 - IR spectra of the samples of solid phase of Mesoniscus graniger graniger (G) and Mesoniscus graniger dragani (D).
Figure 6 from: Giurginca A, Šustr V, Tajovský K, Giurginca M, Matei I (2015) Spectroscopic parameters of the cuticle and ethanol extracts of the fluorescent cave isopod Mesoniscus graniger (Isopoda, Oniscidea). In: Taiti S, Hornung E, Štrus J, Bouchon D (Eds) Trends in Terrestrial Isopod Biology. ZooKeys 515: 111–125. https://doi.org/10.3897/zookeys.515.9395
Figure 6 - FP spectra of the sample of solid phase of Mesoniscus graniger dragani (λex = 265 nm) (Int.- intensity of the peak).
Figure 1 from: Giurginca A, Šustr V, Tajovský K, Giurginca M, Matei I (2015) Spectroscopic parameters of the cuticle and ethanol extracts of the fluorescent cave isopod Mesoniscus graniger (Isopoda, Oniscidea). In: Taiti S, Hornung E, Štrus J, Bouchon D (Eds) Trends in Terrestrial Isopod Biology. ZooKeys 515: 111–125. https://doi.org/10.3897/zookeys.515.9395
Figure 1 - Autofluorescence of the body of Mesoniscus graniger under UV light. a stereomicroscope with UV-inspector 385 (excitation light 365nm) b detail of the antennae - fluorescence microscope U-MWU mirror unit (330–385 nm).
Figure 9 from: Giurginca A, Šustr V, Tajovský K, Giurginca M, Matei I (2015) Spectroscopic parameters of the cuticle and ethanol extracts of the fluorescent cave isopod Mesoniscus graniger (Isopoda, Oniscidea). In: Taiti S, Hornung E, Štrus J, Bouchon D (Eds) Trends in Terrestrial Isopod Biology. ZooKeys 515: 111–125. https://doi.org/10.3897/zookeys.515.9395
Figure 9 - First and second pereionites of Mesoniscus graniger graniger showing the position of tubercles (a); detail of the honeycomb-like net of scales at Mesoniscus graniger dragani (b) (after Giurginca et al. 2012 modified).
Supplementary material 5 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395
SI Figure 5. Boxplots depicting individual measurements of basal membrane labyrinth depth :
Supplementary material 3 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395
SI Figure 3. Boxplots depicting individual measurements of basal lamina thickness :
Supplementary material 2 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395
SI Figure 2. Boxplots depicting individual measurements of cuticle thickness :
Supplementary material 4 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395
SI Figure 4. Boxplots depicting individual measurements of apical membrane labyrinth depth :
Supplementary material 1 from: Bogataj U, Praznik M, Mrak P, Štrus J, Tušek-Žnidarič M, Žnidaršič N (2018) Comparative ultrastructure of cells and cuticle in the anterior chamber and papillate region of Porcellio scaber (Crustacea, Isopoda) hindgut. In: Hornung E, Taiti S, Szlavecz K (Eds) Isopods in a Changing World. ZooKeys 801: 427-458. https://doi.org/10.3897/zookeys.801.22395
SI Figure 1. Boxplots depicting individual measurements of cell size and cell nuclei diameter :
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