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FIGURE 7. A in Limonium ophioides and L. nichoriense (Plumbaginaceae), two new diploid species from Peloponnisos, Greece

FIGURE 7. A. Inner bracts of Limonium coronense, L. pylium, L. nichoriense, and L. ophioides, arranged from left to right. B. Base of stems, with L. coronense on top and L. nichoriense on the bottom. C. Calyces of L. coronense, L. pylium, L. nichoriense, and L. ophioides, displayed from left to right. D. Leaf apex comparison, with L. coronense on the left and L. nichoriense on the right.

FIGURE 1 in Limonium ophioides and L. nichoriense (Plumbaginaceae), two new diploid species from Peloponnisos, Greece

FIGURE 1. Morphological features of Limonium ophioides. A. Habit. B. Leaf. C. Spike. D. Spikelet. E. Calyx. F. Seeds. Illustration by Lydia A. Papadopoulou based on herbarium material from the type locality.

FIGURE 5. Limonium nichoriense A. Habitat. B. Habit. C. Leaves and stems. D in Limonium ophioides and L. nichoriense (Plumbaginaceae), two new diploid species from Peloponnisos, Greece

FIGURE 5. Limonium nichoriense A. Habitat. B. Habit. C. Leaves and stems. D. Detail of the inflorescence.

FIGURE 4 in Limonium ophioides and L. nichoriense (Plumbaginaceae), two new diploid species from Peloponnisos, Greece

FIGURE 4. Morphological features of Limonium nichoriense. A. Habit. B. Leaves. C. Spike. D. Spikelet. E. Calyx. F. Seeds. Illustration by Lydia A. Papadopoulou based on herbarium material from the type locality.

FIGURE 9 in Limonium ophioides and L. nichoriense (Plumbaginaceae), two new diploid species from Peloponnisos, Greece

FIGURE 9. Geographical distribution of Limonium ophioides (green dot), L. nichoriense (blue dot), L. coronense (red dots) and L. pylium (purple dots).

FIGURE 8 in Limonium ophioides and L. nichoriense (Plumbaginaceae), two new diploid species from Peloponnisos, Greece

FIGURE 8. Spikes of Limonium coronense, L. nichoriense, and L. ophioides, arranged from left to right.

FIGURE 4 in Two new diploid species of Isoetes (Isoetaceae: Lycopodiopsida) from Southeastern China based on morphological and molecular evidence

FIGURE 4. Chloroplast phylogenetic tree of Isoetes species. ML bootstrap values are presented under branches. Isoetes malinverniana and Isoetes nuttallii were set as the outgroups.

FIGURE 5 in Two new diploid species of Isoetes (Isoetaceae: Lycopodiopsida) from Southeastern China based on morphological and molecular evidence

FIGURE 5. Phylogenetic tree of the second intron of LEAFY homolog. The circles stand for bootstraps ≥ 70. Isoetes hypsophila was set as the outgroup.

FIGURE 2. Isoetes yuhangensis X. Liu & Y.C. Chen. A in Two new diploid species of Isoetes (Isoetaceae: Lycopodiopsida) from Southeastern China based on morphological and molecular evidence

FIGURE 2. Isoetes yuhangensis X. Liu & Y.C. Chen. A. Proximal view of megaspore. B. Distal view of megaspore. C. Equatorial view of megaspore of. D. Proximal view of microspore of. E. Distal view of microspore. F. Mitotic chromosomes of root tip cells. G. Megasporangium. H. Microsporangium. I. Habitat. Scale bars: A–C = 100 μm; D–E = 5 μm; F = 20 μm; G–H = 2 mm.

FIGURE 1. Isoetes changleensis Y.C. Chen & X. Liu. A in Two new diploid species of Isoetes (Isoetaceae: Lycopodiopsida) from Southeastern China based on morphological and molecular evidence

FIGURE 1. Isoetes changleensis Y.C. Chen & X. Liu. A. Proximal view of megaspore. B. Distal view of megaspore. C. Equatorial view of megaspore. D. Proximal view of microspore. E. Distal view of microspore. F. The mitotic chromosomes of root tip cells. G. Megasporangium. H. Microsporangium. I. Habitat. Scale bars: A–C = 100 μm; D–E = 5 μm; F = 20 μm; G–H = 2 mm.

FIGURE 3 in Phylogenetic relationships among the Iranian Triticum diploid gene pool as inferred from the loci Acc1 and Pgk1

FIGURE 3. Comparison of partial sequences of Pgk1 gene from the Iranian wild diploid Triticum (haplotypes 1−3) and related species. Indels 1 and 2 occurred at positions 54−59 and 475−476, respectively. Indel 3 was found at positions 509−517. Indel 4 was occurred at positions 558−565. The positions of 29 nucleotide substitutions are indicated.

FIGURE 2 in Phylogenetic relationships among the Iranian Triticum diploid gene pool as inferred from the loci Acc1 and Pgk1

FIGURE 2. Comparison of partial sequences of Acc1 gene from the Iranian wild diploid Triticum (haplotypes 1−3) and its affinitive species. Indels 1 and 2 occurred at positions 210–211 and 581−628, respectively. The positions of 4 nucleotide substitutions are indicated.

FIGURE 1 in Phylogenetic relationships among the Iranian Triticum diploid gene pool as inferred from the loci Acc1 and Pgk1

FIGURE 1. Geographic distribution of the 3 haplotypes seen among the wild gene pool of diploid Triticum in Iran.

FIGURE 5 in Phylogenetic relationships among the Iranian Triticum diploid gene pool as inferred from the loci Acc1 and Pgk1

FIGURE 5. Phylogenetic relationships based on Pgk1 sequences among three Iranian haplotypes (1, 2 and 3) of wild diploid Triticum and related genera. This tree topology was obtained in both MP and BI analyses. Branch lengths are proportional to the mean number of substitutions per site as measured by the scale bar. Bayesian posterior probabilities and bootstrap values over 50% are shown above and below the branches, respectively. Sequences obtained from the NCBI are marked with the sequence accession numbers. Secale cereale and Hordeum vulgare sequences were defined as outgroups.

FIGURE 4 in Phylogenetic relationships among the Iranian Triticum diploid gene pool as inferred from the loci Acc1 and Pgk1

FIGURE 4. Phylogenetic relationships based on Acc1 sequences among three Iranian haplotypes (1, 2 and 3) of wild diploid Triticum and related genera. This tree topology was obtained in both MP and BI analyses. Branch lengths are proportional to the mean number of substitutions per site as measured by the scale bar. Bayesian posterior probabilities and bootstrap values over 50% are shown above and below the branches, respectively. Sequences obtained from the NCBI are marked with the sequence accession numbers. Secale cereale and Hordeum vulgare sequences were defined as outgroups.

FIGURE 6 in Molecular, chromosomal and morphological characters reveal a new diploid species in the Smilax china complex (Smilacaceae)

FIGURE 6. Chromosomes of Smilax microdontus sp. nov. and other two closer species: A & B. Smilax sp.nov. 2n=32, (A. mHB, B. mZZ: 2n=32); C. Smilax biflora (bAM: 2n=40); D. Smilax trinervula (tYS: 2n=32).

FIGURE 2 in Molecular, chromosomal and morphological characters reveal a new diploid species in the Smilax china complex (Smilacaceae)

FIGURE 2. Principal component analysis (PCA) of 13 morphological characters of the Smilax china complex. Axes R1 and R2 explain 44.68% and 24.36% of the total variation, respectively.

FIGURE 5. Smilax microdontus Z.S. Sun & C.X in Molecular, chromosomal and morphological characters reveal a new diploid species in the Smilax china complex (Smilacaceae)

FIGURE 5. Smilax microdontus Z.S. Sun & C.X. Fu, sp. nov. A. Fertile branch with fruits; B. Enlarged leaf margin, show the small teeth; C. Narrowly winged petiole; D. Staminate flower; E. Fruit; F. Seed. Drawn by Dr. Xiaofeng Jin.

FIGURE 4 in Molecular, chromosomal and morphological characters reveal a new diploid species in the Smilax china complex (Smilacaceae)

FIGURE 4. Phylogram of the best maximum likelihood tree of the Smilax china complex based on combined nrITS and cpDNA (matK, rbcL, rbcL–aptB intron, and trnS–trnG intron) data. Maximum likelihood and maximum parsimony bootstrap values greater than 50% are presented on the branches.

FIGURE 1 in Molecular, chromosomal and morphological characters reveal a new diploid species in the Smilax china complex (Smilacaceae)

FIGURE 1. Geographical distribution of the Smilax china complex, indicating locations of diploid populations in this study. See Table 1 for population abbreviation.