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6,818 results for “inhibition”
Data for: Optogenetic inhibition of behavior with anion channelrhodopsins
<p>Data for: Potent optogenetic inhibition of behavior with anion channelrhodopsins, a study available at bioRxiv https://doi.org/10.1101/082255</p> <p><strong>Abstract</strong><br> Optogenetics employs light exposure to manipulate physiology in genetically modified organisms. There are abundant tools for optogenetic excitation, but the limitations of current photo-inhibitors present an obstacle to demonstrating the necessity of neuronal circuits. Here we show that anion channelrhodopsins can be used to specifically and rapidly inhibit neural systems involved in Drosophila locomotion, wing expansion, memory retrieval and gustation, demonstrating their broad utility to the circuit analysis of behavior.</p>
Supplementary dataset for journal article "De novo MYC addiction as an adaptive response of cancer cells to CDK4/6 inhibition"
<p>Supplementary material for the journal article "De novo MYC addiction as an adaptive response of cancer cells to CDK4/6 inhibition".<br> 13C Metabolic flux analyses of CDK4/6 knockdown HCT116 cells and control HCT116 cells performed using INCA v1.5 (Young, J. D., 2014, INCA: a computational platform for isotopically non-stationary metabolic flux analysis. Bioinformatics 30, 1333-1335; http://mfa.vueinnovations.com.) running with MATLAB R2012b. The two files were generated by INCA with the estimated flux map distributions, network model, and tracer simulations.</p>
Overcoming clinical resistance to EZH2 inhibition using rational epigenetic combination therapy
<p>Supplementary data for Kazansky et al, "Overcoming clinical resistance to EZH2 inhibition using rational epigenetic combination therapy" and "Epigenetic targeting of PGBD5-dependent DNA damage in SMARCB1-deficient sarcomas." Raw RNA-seq data from G401 cells can be found at the Gene Expression Omnibus (GEO) repository, accession number GSE213845. RNA-seq data from patient tumor samples has been deposited to the Database of Genotypes and Phenotypes (dbGaP), accession number phs003188.v1.p1.</p> <p>All files are labeled with their corresponding figures.</p> <p>"DESeq_processing_FINAL.R" was used for analysis of patient RNA-seq data, using "20230131_SampleTable.csv" and all "*_htseq.txt" files as input.</p> <p>"Tumor_growth_analysis_FINAL_UPDATED" was used for analysis of tumor growth kinetics using the aucVardiTest function. This was used for both of the manuscripts desscribed above.</p>
Real-time influence of intracellular acidification and Na+/H+ exchanger inhibition on in-cell pyruvate metabolism in the perfused mouse heart: A 31P-NMR and hyperpolarized 13C-NMR study
<p>This dataset contains primary data for DOI: 10.1002/nbm.4993</p> <p>NMR in Biomedicine. 2023; 10;e4993</p> <p>Title: Real-time influence of intracellular acidification and Na+/H+ exchanger inhibition on in-cell pyruvate metabolism in the perfused mouse heart: A 31P-NMR and hyperpolarized 13C-NMR study</p> <p>Authors: David Shaul, Naama Lev-Cohain, Gal Sapir, Jacob Sosna, J. Moshe Gomori, Leo Joskowicz, Rachel Katz-Brull</p> <p> </p> <p>Description:</p> <p>These primary datasets contains data presented in the above publication and consist of 31P- (at thermal equilibrium) and hyperpolarized 13C-NMR spectra. </p> <p>Please consult the Archive Guide.</p>
Data and code for "Shrubs inhibit plant diseases through reducing herbaceous biomass in alpine meadows"
<p>Supplementary Data and code for "Shrubs inhibit plant diseases through reducing herbaceous biomass in alpine meadows"</p>
Reactivating PTEN to impair glioma stem cells by inhibiting cytosolic iron-sulfur assembly pathway
<p>Glioblastoma (GBM), the most lethal primary brain tumor, harbors glioma stem cells (GSCs) that not only initiate and maintain malignant phenotypes but also enhance therapeutic resistance. Although frequently mutated in GBMs, the function and regulation of PTEN in PTEN-intact GSCs are unknown. Here we found that PTEN directly interacts with MMS19 and competitively disrupts MMS19-based cytosolic iron-sulfur (Fe-S) cluster assembly (CIA) machinery in the differentiated glioma cells (DGCs). Interrogation of GSCs, when compared with their matched DGCs, revealed that PTEN is specifically succinated at cysteine (C) 211 in GSCs. Isotope tracing coupled with mass spectrometry analysis confirmed that fumarate, generated by adenylosuccinate lyase (ADSL) in <em>de novo</em> purine synthesis pathway which is highly activated in GSCs, promotes PTEN C211 succination. This modification abrogates the interaction between PTEN and MMS19, thereby reactivating CIA machinery pathway in GSCs. Functionally, inhibiting PTEN C211 succination through re-expressing PTEN C211S mutant, depleting ADSL, or consuming fumarate by N-acetylcysteine (NAC), an FDA-approved prescription drug, impairs GSC maintenance. Importantly, re-expressing PTEN C211S or treating with NAC sensitizes GSC-derived brain tumors to temozolomide and irradiation, the standard-of-care treatments for GBM patients, by retarding CIA machinery-mediated DNA damage repair. These findings reveal an immediately practicable strategy to target GSCs for treating GBMs by combined therapy with repurposing NAC.</p>
Data from: Value-related learning in the olfactory bulb occurs through pathway-dependent peri-somatic inhibition of mitral cells
<p>Associating values to environmental cues is a critical aspect of learning from experiences, allowing animals to predict and maximise future rewards. Value-related signals in the brain were once considered a property of higher sensory regions, but their wide distribution across many brain regions is increasingly recognised. Here, we investigate how reward-related signals begin to be incorporated, mechanistically, at the earliest stage of olfactory processing, namely, in the olfactory bulb. In head-fixed mice performing Go/No-Go discrimination of closely related olfactory mixtures, rewarded odours evoke widespread inhibition in one class of output neurons, that is, in mitral cells but not tufted cells. The temporal characteristics of this reward-related inhibition suggest it is odour-driven, but it is also context-dependent since it is absent during pseudo-conditioning and pharmacological silencing of the piriform cortex. Further, the reward-related modulation is present in the somata but not in the apical dendritic tuft of mitral cells, suggesting an involvement of circuit component located deep in the olfactory bulb. Depth-resolved imaging from granule cell dendritic gemmules suggests that granule cells that target mitral cells receive a reward-related extrinsic drive. Thus, our study supports the notion that value-related modulation of olfactory signals is a characteristic of olfactory processing in the primary olfactory area and narrows down the possible underlying mechanisms to deeper circuit components that contact mitral cells peri-somatically.</p>
Supplemental information for: Inhibition of CSF1R and KIT with pexidartinib reduces inflammatory signaling and cell viability in endometriosis
<p>Endometriosis is a common and debilitating disease, affecting ~170 million women worldwide. Affected patients have limited therapeutic options such as hormonal suppression or surgical excision of the lesions, though therapies are often not completely curative. Targeting receptor tyrosine-kinases (RTKs) could provide a nonhormonal treatment option for endometriosis. We determined that two RTKs, Macrophage colony stimulating factor receptor (CSF1R) and Mast/stem cell growth factor receptor KIT (KIT), are overexpressed in endometriotic lesions and could be novel nonhormonal therapeutic targets for endometriosis. The kinase activity of CSF1R and KIT is suppressed by pexidartinib, a small molecule inhibitor that was recently approved by the US Food and Drug Administration (FDA). Using immunohistochemistry, we detected CSF1R and KIT in endometriotic tissues obtained from peritoneal lesions, colorectal lesions, and endometriomas. Specifically, we show that KIT is localized to the epithelium of the lesions, while CSF1R is expressed in the stroma and macrophages of the endometriotic lesions. Given the high epithelial expression of CSF1R and KIT, 12Z endometriotic epithelial cells were used to evaluate the efficacy of dual CSF1R and KIT inhibition with pexidartinib. We found that pexidartinib suppressed activation in 12Z cells of JNK, STAT3 and AKT signaling pathways, which control key pro-inflammatory and survival networks within the cell. Using quantitative real time PCR, we determined that pexidartinib suppressed interleukin 8 (<em>IL8) </em>and cyclin D1 (<em>CCND1) </em>expression<em>.</em> Lastly, we demonstrated that pexidartinib decreased cell growth and viability.<em> </em>Overall, these results indicate that pexidartinib-mediated CSF1R and KIT inhibition reduces pro-inflammatory signaling and cell viability in endometriosis.</p>
Data from: DGKα/ζ inhibition lowers the TCR affinity threshold and potentiates anti-tumor immunity
<p>Checkpoint blockade immunotherapies expand neoantigen- or virus-specific T cells, and poor responsiveness to immunotherapy is associated with lower mutational burden in tumors of non-viral origin. Although mouse models demonstrate that lower affinity T cells recognizing self-antigens can contribute to tumor control if sufficiently activated, therapeutic options for enhancing T cell priming are limited. Diacylglycerol kinases (DGKs) attenuate DAG signaling by converting DAG to phosphatidic acid, thereby suppressing pathways downstream of TCR signaling. Using a novel dual DGK alpha and zeta inhibitor (DGKi), tumor-specific CD8 T cells with different affinities (TRP1high and TRP1low), and a series of altered peptide ligands, we demonstrate that inhibition of DGKα/ζ can lower the signaling threshold for T cell priming. TRP1high and TRP1low CD8 T cells produced more IL-2, IFNγ, and other effector cytokines in the presence of cognate antigen and DGKi. Effector TRP1high- and TRP1low-mediated cytolysis of tumor cells with low antigen load was MHC-restricted, mediated by IFNγ, and augmented by DGKi. Adoptive T cell transfer into mice bearing pancreatic or melanoma tumors synergized with single-agent DGKi or DGKi and αPD1, with increased expansion of low-affinity T cells and increased cytokine production observed in tumor infiltrates of treated mice. Collectively, our findings highlight DGKα/ζ as therapeutic targets for augmenting tumor-specific CD8 T cell function.</p>
Feedback Inhibition of DszC, a Crucial Enzyme for Crude Oil Biodessulfurization
<p>Raw data for docking calculations ran with AutoDock Vina 4</p> <p>Raw data for conventional MD simulations ran with Gromacs 2018.3</p> <p>Input files to reproduce all calculations and simulations performed in the work</p> <p>Code written to perform data analysis and represenation</p> <p>Raw data for the analyses leading to the presented and discussed results in the manuscript</p> <p> </p>
Inhibition of gut digestive proteases by cyanobacterial diets decreases infection in a Daphnia host-parasite system
<p>Secondary metabolites produced by primary producers have a wide range of functions as well as indirect effects outside the scope of their direct target. Research suggests that protease inhibitors produced by cyanobacteria influence grazing by herbivores and may also protect against parasites of cyanobacteria. In this study we asked whether those same protease inhibitors produced by cyanobacteria also can influence interactions of herbivores with their parasites. </p> <p>We used the <em>Daphnia-Metschnikowia</em> zooplankton host-fungal parasite system to address this question because it is well-documented that cyanobacteria protease inhibitors suppress trypsin and chymotrypsin in the gut of <em>Daphnia</em>, and because it is known that <em>Metschnikowia</em> infects via the gut. We tested the hypothesis that <em>Daphnia</em> gut proteases are necessary for <em>Metschnikowia</em> spores to be released from their asci. We then also tested whether diets that decrease trypsin and chymotrypsin activity in the guts of <em>Daphnia</em> lead to lower levels of infection.</p> <p>Our results show that chymotrypsin promotes release of the fungal spores from their asci. Moreover, a diet that strongly inhibited chymotrypsin activity in <em>Daphnia</em> decreased infection levels, particularly in the most susceptible <em>Daphnia</em> clones.</p> <p>Our results support the growing literature that cyanobacterial diets can be beneficial to zooplankton hosts when challenged by parasites and uncover a mechanism that contributes to the protective effect of cyanobacterial diets. Specifically, we demonstrate that host chymotrypsin enzymes promote dehiscence of <em>Metschnikowia</em> spores; when cyanobacteria inhibit activity of chymotrypsin in hosts, this most likely traps the spore inside the ascus, preventing the parasite from puncturing the gut and beginning the infection process, and reduced the proportion of <em>Daphnia</em> infected.</p> <p>This study illustrates how secondary metabolites of phytoplankton can protect herbivores against their own enemies.</p>
Supplement Tables of Recombinant Klotho protein protects pulmonary alveolar epithelial cells against sepsis-induced apoptosis by inhibiting the Bcl-2/Bax/caspase-3 pathway
<p>This is Supplement Tables of Recombinant Klotho protein protects pulmonary alveolar epithelial cells against sepsis-induced apoptosis by inhibiting the Bcl-2/Bax/caspase-3 pathway.</p>
Dataset (I.) related to publication: PIP4K2C inhibition reverses autophagic flux arrest induced by SARS-CoV-2
<p>MD simulation data (PIKfyve simulations) related to publication: </p> <p>Karim, M., Mishra, M., Lo, CW. <em>et al.</em> PIP4K2C inhibition reverses autophagic flux impairment induced by SARS-CoV-2. <em>Nat Commun</em> <strong>16</strong>, 6397 (2025). https://doi.org/10.1038/s41467-025-61759-1</p> <ul> <li>The .zip files contain raw Desmond simulation trajectories of PIKfyve in complex with RMC-113 [18 replicas; each 4 us] (-out.cms files and trajectories).</li> </ul> <p> </p>
Inhibition of DKK-1 Limits Osteosarcoma Metastasis in a Clinically Relevant Mouse Model
<p>Single-cell RNA-seq data of untreated (F43N, F43R) and DKK-1 inhibitor treated (OSW3, OSW4, OSW5, OSW6) patient derived xenografts (PDXs). </p> <p>human_cells_scanpy_object.h5ad -- contains Scanpy analysis of human cells from untreated and DKK-1 inhibitor treated PDXs</p> <p>raw_counts_cellranger_output.zip -- contains the raw expression counts of untreated and DKK-1 inhibitor treated PDXs cells outputted by CellRanger. </p>
Dataset used in "Helper NLR immune protein NRC3 evolved to evade inhibition by a cyst nematode virulence effector"
<p><strong>[Figs 1 and S2]</strong></p> <p> </p> <p><strong>00_cloned_NRC123.fasta</strong></p> <p> </p> <p>FASTA file containing NRC1, NRC2 and NRC3 sequences tested in HR cell death assay.</p> <p> </p> <p><strong>01_NRCX0123_4species.fasta</strong></p> <p> </p> <p>FASTA file containing NRC0, NRC1, NRC2, NRC3 and NRCX of <em>N. benthamiana</em>, <em>C. annuum</em> (pepper), <em>S. tuberosum</em> (potato) and <em>S. lycopersicum</em> (tomato). In addition to a previously published dataset (Selvaraj et al., 2023), we included the NbNRC2, CaNRC3 and StNRC3 sequences from 00_cloned_NRC123.fasta.</p> <p> </p> <p><strong>02_NRCX0123_4species.local_aln.fasta</strong></p> <p> </p> <p>FASTA file containing the protein sequence alignment of 01_NRCX0123_4species.fasta. We used MAFFT for the alignment (Katoh & Standley, 2013).</p> <p> </p> <p><strong>03_NRCX0123_4species.local_aln.clip.fasta</strong></p> <p> </p> <p>FASTA file containing the trimmed protein sequence alignment of 02_NRCX0123_4species.local_aln.fasta. We used ClipKIT for trimming (Steenwyk et al., 2020).</p> <p> </p> <p><strong>04_NRCX0123_4species.local_aln.clip.fasta.treefile</strong></p> <p><strong> </strong></p> <p>Newick file containing the phylogenetic tree reconstructed based on 03_NRCX0123_4species.local_aln.clip.fasta. We used IQ-TREE to create a phylogenetic tree (Minh et al., 2020).</p> <p> </p> <p><strong>[Fig 2B]</strong></p> <p><strong> </strong></p> <p><strong>05_cloned_NRC123.local_aln.fasta</strong></p> <p><strong> </strong></p> <p>FASTA file containing the protein sequence alignment of 00_cloned_NRC123.fasta. We used MAFFT for the alignment (Katoh & Standley, 2013).</p> <p> </p> <p><strong>[Fig 5 and Table S1]</strong></p> <p> </p> <p><strong>06_NRCH_cds_23-06-20.min2400max2800.fasta</strong></p> <p><strong> </strong></p> <p>FASTA file containing the nucleotide sequences of helper NRC sequences from 124 Solanaceae genomes (Sugihara et al., 2023; Huang et al., 2023). We filtered out sequences shorter than 2,400 or longer than 2,800 bases, resulting in 1,748 sequences.</p> <p> </p> <p><strong>07_NRCH_cds_23-06-20.min2400max2800.aa.fasta</strong></p> <p> </p> <p>FASTA file of the amino acid sequences translated from 06_NRCH_cds_23-06-20.min2400max2800.fasta.</p> <p> </p> <p><strong>08_NRCH_cds_23-06-20.min2400max2800.aa.NBARC.fasta</strong></p> <p> </p> <p>FASTA file containing the amino acid sequences of NB-ARC module corresponding to the sequences in 07_NRCH_cds_23-06-20.min2400max2800.aa.fasta.</p> <p> </p> <p><strong>09_NRCH_cds_23-06-20.min2400max2800.aa.NBARC.local_aln.clip.fasta</strong></p> <p> </p> <p>FASTA file containing the trimmed protein sequence alignment of 02_NRCX0123_4species.local_aln.fasta. We used MAFFT and ClipKIT for the alignment and trimming, respectively (Katoh & Standley, 2013; Steenwyk et al., 2020).</p> <p> </p> <p><strong>10_NRCH_cds_23-06-20.min2400max2800.aa.NBARC.local_aln.clip.fasta.treefile</strong></p> <p> </p> <p>Newick file containing the phylogenetic tree reconstructed based on 09_NRCH_cds_23-06-20.min2400max2800.aa.NBARC.local_aln.clip.fasta. We used IQ-TREE to create a phylogenetic tree (Minh et al., 2020).</p> <p> </p> <p><strong>11_NRCX123_cds_23-06-20.min2400max2800.fasta</strong></p> <p> </p> <p>FASTA file containing the the nucleotide sequences of NRC1/2/3X clades identified based on 10_NRCH_cds_23-06-20.min2400max2800.aa.NBARC.local_aln.clip.fasta.treefile.</p> <p> </p> <p><strong>12_NRCX123_nt_codon_ancseq_v1.2.1.zip</strong></p> <p> </p> <p>Results of ancestral sequence reconstruction. We used ancseq to perform ancestral sequence reconsturction (Sugihara, 2024). "NRCX123_cds_23-06-20.min2400max2800.nt_codon.local_aln.manual.clip.uniq.rm_4sp.fasta" is an input alignment and "NRCX123_cds_23-06-20.min2400max2800.nt_codon.local_aln.manual.clip.uniq.rm_4sp.fasta.treefile" is a tree file. Regarding the output files for ancseq, please refer to the <a href="https://github.com/YuSugihara/ancseq?tab=readme-ov-file#outputs">GitHub repository</a>.</p> <p> </p> <p><strong>[Fig S7]</strong></p> <p> </p> <p><strong>13_logo_plot.zip</strong></p> <p> </p> <p>Sequence alignments and script used in Fig S7. To generate the consensus sequence shown in Fig S7, we concatenated interfaces 1, 2 and 3 with SS15 and visualized the results using logomaker (Tareen and Kinney, 2020).</p> <p> </p> <p><strong>References</strong></p> <p> </p> <p>Huang C-Y, Huang Y-S, Sugihara Y, Wang H-Y, Huang L-T, Lopez-Agudelo JC, Chen Y-F, Lin K-Y, Chiang B-J, Toghani A, Kourelis J, Derevnina L, Wu C-H. 2023. Functional divergence shaped the network architecture of plant immune receptors. <em>bioRxiv</em>. 2023:2023.12.12.571219. DOI: 10.1101/2023.12.12.571219.</p> <p>Katoh K, Standley DM. 2013. MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. <em>Molecular Biology and Evolution</em> 30:772–780. DOI: 10.1093/molbev/mst010.</p> <p>Minh BQ, Schmidt HA, Chernomor O, Schrempf D, Woodhams MD, von Haeseler A, Lanfear R. 2020. IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era. <em>Molecular Biology and Evolution</em> 37:1530–1534. DOI: 10.1093/molbev/msaa015.</p> <p>Selvaraj M, Toghani A, Pai H, Sugihara Y, Kourelis J, Yuen ELH, Ibrahim T, Zhao H, Xie R, Maqbool A, Concepcion JCD la, Banfield MJ, Derevnina L, Petre B, Lawson DM, Bozkurt TO, Wu C-H, Kamoun S, Contreras MP. 2023. Activation of plant immunity through conversion of a helper NLR homodimer into a resistosome. <em>bioRxiv</em>. 2023:2023.12.17.572070. DOI: 10.1101/2023.12.17.572070.</p> <p>Steenwyk JL, Iii TJB, Li Y, Shen X-X, Rokas A. 2020. ClipKIT: A multiple sequence alignment trimming software for accurate phylogenomic inference. <em>PLOS Biology</em> 18:e3001007. DOI: 10.1371/journal.pbio.3001007.</p> <p>Sugihara Y. 2024. YuSugihara/ancseq: v1.2.1. <em>Zenodo</em>. DOI: 10.5281/zenodo.10808871.</p> <p>Sugihara Y, Toghani A, Kamoun S, Kourelis J. 2023. NLRome dataset from 124 genomes of plants in the Solanaceae family. <em>Zenodo</em>. DOI: 10.5281/zenodo.10354350.</p> <p>Tareen A, Kinney JB. 2020. Logomaker: beautiful sequence logos in Python. Bioinformatics 36:2272–2274. doi:10.1093/bioinformatics/btz921</p> <p> </p>
Dual FAAH/MAGL inhibition in migraine pain
<p>This dataset comprises the findings obtained in the study aimed at investigating the effect of dual inhibition of anandamide and 2 arachidonyl glycerol (2-AG) catabolic pathways (FAAH and MAGL respectively) in the nitroglycerin (NTG)-based animal model of migraine. The dual inhibitor JZL195 was administered to male rats 2h after NTG or vehicle injection. Rats were then exposed to the open field and the orofacial formalin tests 4 hours after NTG or vehicle. At the end of the evaluations, they were sacrificed to evaluate calcitonin gene-related peptide (CGRP) serum levels and gene expression of CGRP and pro-inflammatory cytokines in the cervical spinal cord and the trigeminal ganglion.</p> <p>We also investigated the effect of subtype-selective antagonist for cannabinoid receptors 1 and 2 (AM251 and AM630, respectively) on the behavioral JZL195 effects. The dual inhibitor significantly reduces the NTG-induced trigeminal hyperalgesia and pain-associated behavior, possibly via cannabinoid 1 receptors-mediated action, but it did not change the hypomotility and the anxiety behaviors induced by NTG. The decreased hyperalgesia was associated with a reduction in CGRP and cytokine gene expression levels in central and peripheral structures and reduced CGRP serum levels. These data suggest an antinociceptive synergy of the endocannabinoid action in peripheral and central sites, confirming that this system participates in reduction of cephalic pain signals.</p> <p> </p> <p>The <em>in vivo</em> and <em>ex vivo</em> assessments were:</p> <ol> <li>Distance (expressed in meters) travelled in the apparatus, time spent (expressed in seconds) in the center of the apparatus, number of rearing, time spent in grooming behavior (expressed in seconds) were evaluated in the open field test. Each animal was placed in a 92 x 92 cm arena and video recorded for 10 minutes. The analysis was done manually (for rearing and grooming) and with the ANY-Maze software (for total distance and time in the centre).</li> <li>Pain-related behavior in the orofacial formalin test: the face rubbing was measured counting the seconds the animal spent grooming the injected area (upper lip, lateral to the nose) with the ipsilateral forepaw or hindpaw 0–6 min (Phase I) or 12–45 min (Phase II) after formalin injection (50 µl, s.c.). The observation time was divided into 15 blocks of 3 min each.</li> <li>mRNA expression levels: calcitonin gene-related peptide (CGRP), interleukin-6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha) mRNA were evaluated in cervical spinal cord and trigeminal ganglia. mRNA levels were measured by rt-PCR. All samples were assayed in triplicate and gene expression levels were calculated according to 2−∆∆Ct = 2− (∆Ct gene − ∆Ct housekeeping gene) formula by using Ct (cycle threshold) values.</li> <li>CGRP protein levels in serum: the blood samples were collected in clot activator with gel separator serum tubes and centrifugated for 15 min at 1000×g at 2-8° C. Serum CGRP levels were measured using a commercial enzyme-linked immuno-sorbent assay (ELISA) kit (Elabsciences). Data are expressed as pg/ml.</li> </ol> <p>Results: The dual inhibitor significantly reduced the NTG-induced trigeminal hyperalgesia and pain-associated behavior, possibly via cannabinoid 1 receptors-mediated action, but it did not change the hypomotility and the anxiety behaviors induced by NTG. The decreased hyperalgesia was associated with a reduction in CGRP and cytokine gene expression levels in central and peripheral structures and reduced CGRP serum levels.</p>
Supporting data and code for: A high diversity of mechanisms endows ALS-inhibiting herbicide resistance in the invasive common ragweed (Ambrosia artemisiifolia L.)
<p>This is the first release of the final data and code for the article accepted for publication in Scientific Reports journal. It contains all the necessary scripts to produce the maps of the manuscript. All the necessary data can be found in the 'data' folder.</p>
Aplication of eco-enzyme from nutmeg, clove, and eucalyptus plant waste in inhibiting the growth of E. coli and S. aureus
<p>That different plant wastes' eco-enzymes also had distinctive colors, where DP and DK were brown, BP was reddish-brown, while DC appeared blackish-brown and clear. These differences occur due to variations in the chemical composition of each material used. Furthermore, the acidic aroma from each eco-enzyme was derived from the decomposition of alcohol compounds into acetic acid during aerobic respiration. The aroma was distinctively different depending on the type of plant waste used. Eco-enzymes and commercial antiseptics also have different abilities to inhibit <em>E. coli</em> and <em>S. aureus </em>growth with the highest inhibition found in eco-enzymes made from eucalyptus leaf waste</p>
DFT Calculation Data for "Stabilized tilted-octahedra halide perovskites inhibit local formation of performance-limiting phases"
<p>Initial and optimized structures that are used for DFT simulations associated with Fig 2A-G in <em>Science</em> <strong>374</strong>, 1598 (2021) (DOI: 10.1126/science.abl4890)</p>
Supplemetary data for the paper "Knockdown of circular RNA hsa_circ_0003307 inhibits synovial inflammation in ankylosing spondylitis by regulating the PI3K/AKT pathway" by Yanyan Fang, Jian Liu*, Yan Long, Jianting Wen, Dan Huang, Ling Xin
<p>Statistical analyses and graphs were performed using Prism 8 software (GraphPad, La Jolla, USA). Age was analyzed using Student's t test (Supplementary Table 1A,B). Expression of circRNA_0003307 in PBMCs was analyzed using Welch's t-test (Supplementary Table 2A,B). CircRNA_0003307 expression in AS-FLS was analyzed using a one-way analysis of variance, then Games-Howell test (Supplementary Table 4A–C). The p-PI3K protein expression in AS-FLS was analyzed using a one-way analysis of variance, then Games-Howell test (Supplementary Table 5A–C). The p-AKT protein expression in AS-FLS was analyzed using a one-way analysis of variance, then Games-Howell test (Supplementary Table 6A–C). The TNF-α levels in AS-FLS was analyzed using a one-way analysis of variance, then Games-Howell test (Supplementary Table 7A–C). The TNFAIP2 levels in AS-FLS was analyzed using a one-way analysis of variance, then Games-Howell test (Supplementary Table 8A–C). Categorical variables were compared using chi-square test. Correlations were assessed using Spearman’s analysis because clinical characteristics were not normally distributed (Supplementary Table 3). A receiver operating characteristic (ROC) curve analysis was performed to judge whether circRNA_0003307 can be used as a diagnostic indicator for AS. P <0.05 indicates that the difference was statistically significant.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.