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Dataset results
130 results for “live imaging”
Live cell imaging reveals the trade-off between target search flexibility and efficiency for Cas9 and Cas12a
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Live cell imaging data of IRE1a-GFP foci formation
<p>Live cell imaging of XBP1 mRNA and IRE1a-GFP expressing cells.</p>
Live imaging data of XBP1 mRNA under ER stress and IRE1a inhibition
<p>Live microscopy data of XBP1 mRNA transcript in TM and 4u8C.</p>
Live imaging data of XBP1 mRNA under control conditions.
<p>Live imaging data of single XBP1 mRNA transcripts under control conditions.</p>
Live imaging data of XBP1 mRNA under ER stress conditions.
<p>Live microscopy images of single XBP1 mRNA transcripts under ER stress conditions (TM).</p>
Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe
<p>Raw imaging data and source data in the maniscript.</p>
CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies
<p><strong>Abstract:</strong></p> <p>We introduce CALIPERS, a Fluorescence Ubiquitin Cell Cycle Indicator enabling cell cycle-aware phenotyping based on multiplexed live-cell imaging. We validate CALIPERS in three- and four-color reporter lines (HaCaT) and human induced pluripotent stem cells (WTC-11), co-expressing genetically encoded structural and functional fluorescent sensors. To exemplify the broad applications of CALIPERS, we show cell cycle-aware phenotyping in live-cell imaging applications ranging from proliferation and migration to cardiac-specific maturation and drug testing.</p> <p>Code submission: <a href="https://github.com/alessandro-bertero/FUCCIplex">https://github.com/alessandro-bertero/FUCCIplex.git</a></p> <p><strong>Dataset description:</strong></p> <ol> <li>This dataset includes all data and the additional files to recreate the sc-RNAseq analysis in our manuscript. The Rmd files include the Seurat QC pipeline to preprocess the counts data from cellranger (<strong>QC_Seurat_gitgub_polished.RmD</strong>) and the monocle pipeline to re-create the clusters and pseudotime analysis (<strong>monocle_github_polished.Rmd</strong>).</li> <li><strong>Data obtained from Cellranger 7.2 pipeline</strong> are also available here including all the 3 files, usually placed in the path "outs/count/filtered_feature_bc_matrix" by cellrancer. This includes: <strong>barcodes.tsv.gz, features.tsv.gz and matrix.mtx.gz</strong> (alternatively the h5 file filtered_feature_bc_matrix.h5 can be loaded directly in Seurat v5).</li> <li>We also added few RData files to recreate our graphs and follow our pipeline smoothly. <strong>240111_seurat_before_clean.RData, 240111_seurat_clean.RData</strong> and <strong>240111_seurat_processed.RData</strong> are the files created by the codes in QC_Seurat_gitgub_polished.RmD pipeline. Then, <strong>240118_monocle_cds_clustered_proteincoding_PSEUDOTIME_clean_gene_info.RData</strong> is the file set created by the<strong> </strong>monocle_github_polished.Rmd pipeline.</li> </ol> <p>We also suggest to use this pipeline within a docker container by pulling our docker image with R studio:</p> <p><strong>docker pull hedgelab/rstudio-hedgelab:latest</strong></p>
Data from: Long-term live imaging of the Drosophila adult midgut reveals real-time dynamics of division, differentiation, and loss
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Data from: Metamorphosis revealed: time-lapse three-dimensional imaging inside a living chrysalis
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Data from: Live imaging of symbiosis: spatiotemporal infection dynamics of GFP-labelled Burkholderia symbiont in the bean bug Riptortus pedestris
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Quantitative live-cell imaging of secretion activity reveals dynamic immune responses
GEO Series GSE253100. Homo sapiens. 7 samples. Type: Expression profiling by high throughput sequencing.
Next-generation breast organoids capture human organogenesis with high-resolution live imaging
GEO Series GSE274346. Homo sapiens. 1 samples. Type: Expression profiling by high throughput sequencing.
Live-animal imaging of native hematopoietic stem and progenitor cells
GEO Series GSE115908. Mus musculus. 2 samples. Type: Expression profiling by high throughput sequencing.
Live imaging in freely behaving mice reveals that adult neural stem cell activation is regulated by the day/night cycle and intracellular Ca2+ dynamic
GEO Series GSE161276. Mus musculus. 4 samples. Type: Expression profiling by high throughput sequencing.
Ultra-resolution, multiscale live imaging reveals TADs as domains of cohesin-accelerated search
GEO Series GSE289566. Mus musculus. 13 samples. Type: Expression profiling by high throughput sequencing.
Neuronal Uptake Live Imaging Data 2N4R Tau
<p>Live imaging videos for neuronal uptake of 2N4R tau glycoproteins labeled with 10% pHrodo-red at 50 nM concentration over 24 h (brigtfield, fluorescence, and merge channels).</p>
Dataset of live imaging of RNA Polymerase II CTD phosphorylation in zebrafish embryos
<p>Images are recruited RNA polymerase II Serine 5 (Pol II Ser5P) and RNA polymerase II Serine 2 (Pol II Ser2P) in live sphere stage zebrafish embryos, visualized with antibody fragments (Fab) labelled with Janelia Fluor 647 and Alexa Fluor 488, respectively.<br> <br> Dataset "<strong>EmbryoC_20ms_040.nd2" </strong>is recorded by 20 ms exposure time and contains 120 frames. Dataset "<strong>EmbryoC_50ms_042.nd2</strong>" contains images recorded with 50 ms exposure time and contains 61 frames. </p>
Use of Mobile Phones for Thermal Imaging of the Chest to Save Lives of Children With Pneumonia in Nagpur, India
ClinicalTrials.gov study NCT02381431. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Functional Magnetic Resonance Imaging (fMRI) and Robot-Assisted Practice of Activities of Daily Living
ClinicalTrials.gov study NCT00878085. IPD Sharing: Not stated. Countries: 1. Publications: 0.
The Effect of Showing Photographs, Videos and Live Images of Their Babies to Mothers During Milking
ClinicalTrials.gov study NCT05700162. IPD Sharing: NO. Countries: 1. Publications: 0.
ScienceDex guides
Understand access before you commit
These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.