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8,565 results for “characterization”
Laser Beam Characterization Data Related to Testing Different Near-Infrared Laser Therapy Devices Used in Sports Medicine
<p>This dataset contains laser beam characterization data collected for different near-infrared laser therapy devices that are used in sports medicine. Data were collected between September 2021 and December 2022 at the Department of Anatomy II (Neuroanatomy) of the Ludwig-Maximilians University of Munich, Germany. Laser therapy devices were characterized using a thermal power sensor, a photodiode, and a beam profiling camera (for details, see [1]). Two different laser therapy devices were investigated (Dolorclast High Power Laser, Electro Medical Systems; Cube 4 Med, Eltech K-laser s.r.l.). The measurements were repeated for three units of each laser therapy device (EMS-1, EMS-2, EMS3; K-1, K-2, K-3). The dataset contains raw data files from each sensor that include text (TXT) files, comma-separated-values (CSV) formatted files, and raw camera recordings in binary format (BINARY.BGDATA). The data was used to create figures that were submitted to the journal MDPI Biomedicines [1]. The figures in [1] were created with Python in a Jupyter Notebook, which is also included in this dataset.</p> <p><strong>2023-02-17 Update: The manuscirpt has been published in MDPI Biomedicines [2]. If you make any use of this dataset, please cite [2].</strong></p> <p>[1] Kaub, L.; Schmitz, C. Spread of the Optical Power Emission of Several Units of the Same Laser Therapy Devices Used in Sports Medicine, Which Cannot Be Assessed by the Users, Shown by Means of High-Fidelity Laser Measurement Technology. <em>Preprints</em> <strong>2023</strong>, <em>2023010251</em>, https://doi.org/10.20944/preprints202301.0251.v1.</p> <p>[2] Kaub, L.; Schmitz, C. Spread of the Optical Power Emission of Three Units Each of Two Different Laser Therapy Devices Used in Sports Medicine, Which Cannot Be Assessed by the Users, Shown by Means of High-Fidelity Laser Measurement Technology. <em>Biomedicines</em> <strong>2023</strong>, <em>11</em>, 585. https://doi.org/10.3390/biomedicines11020585</p>
SYNTHESIS, CHARACTERIZATION AND ECOTOXICOLOGICAL EVALUATION OF Ag3VO4/Ag NANOCOMPOSITE
<p>Historically, the development of new methods of synthesizing materials has been linked to the development of societies, mainly due to the ability to promote changes in materials and enable new types of technological applications. Although there is quite sophisticated content about pulsed lasers in femtoseconds, there is still a need to understand the mechanisms of formation and transformation of semiconductor materials, as well as to verify the changes provided, in a more appropriate way, when synthesized by this technique. However, such development must always be combined with the need to minimize and verify environmental effects. This study had as its main objectives to synthesize Ag<sub>3</sub>VO<sub>4</sub> via the microwave-assisted hydrothermal method, in different synthesis times (t: 2, 4, 8, 16, 32, 64 min); irradiate the Ag3VO4 samples using a pulsed laser in femtoseconds to form the composite; characterize particulate composites; as well as to evaluate the ecotoxicity of the composite on the floating aquatic macrophyte species <em>Ricciocarpus natans</em>. The powders obtained from the composites were structurally characterized by the techniques of X-ray diffraction, Raman scattering spectroscopy and UV-Vis electronic spectroscopy, and had the surface characterized by scanning and transmission electron microscopy techniques, allowing the identification of structural alterations and surface. Theoretical calculations were used to understand the geometric, vibrational, electronic and charge distribution structures at the atomic level and the nature of chemical interactions present in semiconductor structures. In addition, toxicity bioassays were carried out until the 42nd day in order to obtain an analysis of the ecotoxicological effects, with the aid of mathematical modeling, of these particles on the bioindicator <em>Ricciocarpus natans</em>.</p> <p><strong>Electron Charge Density: 2D Map</strong></p> <p>Raw theoretical electron charge density files which are used to create 2D difference maps for Ag<sub>3</sub>VO<sub>4</sub> and AgVO<sub>3</sub> used in the main manuscript</p> <p><strong>Bader Charges</strong></p> <p>Raw theoretical Bader charges files for Ag<sub>3</sub>VO<sub>4</sub> and AgVO<sub>3</sub> used in the main manuscript</p> <p><strong>Band Structure and Density of States</strong></p> <p>Raw band structure and density of states files for Ag<sub>3</sub>VO<sub>4</sub> and AgVO<sub>3</sub> used in the main manuscript</p> <p><strong>Bioassays</strong></p> <p>Raw bioassays files for composite used in the main manuscript</p> <p><strong>Raman</strong></p> <p>Raw Raman files for Ag<sub>3</sub>VO<sub>4</sub> and composite and theoretical raw Raman files for Ag<sub>3</sub>VO<sub>4</sub> and AgVO<sub>3</sub> used in the manuscript</p> <p><strong>Rietveld Refinement</strong></p> <p>Raw Rietveld refinement files for Ag<sub>3</sub>VO<sub>4</sub> used in the manuscript</p> <p><strong>SEM</strong></p> <p>SEM images for Ag<sub>3</sub>VO<sub>4</sub> and composite used in the manuscript</p> <p><strong>TEM</strong></p> <p>TEM and EDX spectres and maps images for Ag<sub>3</sub>VO<sub>4</sub> and composite and nanodiffraction image for composite used in the manuscript</p> <p><strong>Optimized Structure</strong></p> <p>Raw optimized structure files for Ag<sub>3</sub>VO<sub>4</sub> and AgVO<sub>3</sub> used in the manuscript</p> <p><strong>TOPOND</strong></p> <p>Raw TOPOND files for Ag<sub>3</sub>VO<sub>4</sub> and AgVO<sub>3</sub> used in the manuscript</p> <p><strong>UV-Vis Spectroscopy</strong></p> <p>Raw UV-Vis files for Ag<sub>3</sub>VO<sub>4</sub> and AgVO<sub>3</sub> used in the manuscript</p> <p><strong>XRD</strong></p> <p>Raw XRD files for Ag<sub>3</sub>VO<sub>4</sub> and composite used in the manuscript</p> <p>Note: The words "composite" and "2min_irra or 64 min_irra" are used for the same kind of sample. They are synonymous in this work.</p> <p>Funding provided by: grant #2020/11232-3, São Paulo Research Foundation (FAPESP).</p> <p><em>This</em><em> </em><em>study</em><em> </em><em>was</em><em> </em><em>financed in part</em><em> </em><em>by</em><em> </em><em>the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance</em><em> Code 001.</em></p>
AALTO - Channel Characterization at Sub-THz Band with Measurements and Ray Tracing in Indoor Case - DATA
<p>The data set includes simulation results from radio propagation modelling of TERAWAY links (at 90, 95 and 100 GHz) in realistic university corridor environment. The modelling is performed using a Ray Tracing Tool developed in MATLAB environment at Aalto University. Ray tracing technique used in this tool is based on Image Theory (IT) algorithm. Unlike a quasi three-dimensional environment, it supports ray tracing in full three dimension.</p> <p>This data set contains propagation modelling results of the TERAWAY link. Output data includes (but is not limited to): Multipath component IDs, Path Distance (meter), Angle of Arrival AoA (degree), Angle of Departure AoD (degree), Direction of Arrival DoA (degree), Direction of Departure DoD (degree), E-Field (Volt/meter), H-Field (Ampere/meter), Phase (Radians), Power (Watts), Number of reflections a path experienced, Number of diffractions a path experienced, information that is it ground reflected path or not, Receiver location (x and y coordinates), information that is it rooftop path or not.</p>
Materials characterization and adsorption isotherm data
<p><span>Nano-zirconia (ZO) was synthesized using a microwave assisted one pot precipitation route. Two biopolymers, chitosan (CTS) and carboxymethyl cellulose (CMC), were then combined with zirconia at different w/w ratios in order to identify the system with the most improved sorption properties towards Pb(II), Cd(II), As(V) and F<sup>-</sup>. The formulation with 30% w/w chitosan (ZO-CTS) was found to give enhanced uptake of F<sup>-</sup> and As(V). Improved sorption properties could not be observed for Pb(II) and Cd(II) in any of the ZO-polymer blends, with ZO being the most potent sorbent here. ZO and the lead system ZO-CTS were characterized in detail using Fourier transform infra-red spectroscopy, scanning electron microscopy, X-ray diffraction and X-ray photoelectron spectroscopy (XPS). These confirmed the formation of a composite system comprising nanoparticles with 50 nm in size in which ZO was present in the amorphous form. Interactions between Zr and the amide and hydroxide groups of chitosan were suggested from XPS. Batch adsorption studies were carried out and equilibrium adsorption data were fitted to a series of isotherm models. It was observed that the combination of ZO with CTS improved the F<sup>-</sup> and As(V) adsorption capacity most notably at pH 5.2. Fluoride adsorption by ZO-CTS followed the Freundlich isotherm model, with an adsorption capacity of 120 mg/g. Adsorption of As(V) by ZO-CTS could be fitted with both the Langmuir and Freundlich isotherm models, and was found to have a capacity of 14.8 mg/g. Gravity filtration studies were also carried out at two different pH levels to test the feasibility of ZO-CTS in real-world applications. These indicated that the sorbent could be used to filter a significant volume of water and ensure that pollutant levels remained below acceptable values.</span></p>
Proteogenomic Characterization of Cholangiocarcinoma
<p>supplemental table of Integrated Proteogenomic Characterization of Cholangiocarcinoma Associated with Clinical Outcomes</p>
Smart characterization of heterogeneous catalysts: EXAFS for Single Atom Catalyst
<p>This repository houses the data and metadata generated as part of the "Smart characterization of heterogeneous catalysts: EXAFS for Single Atom Catalyst" project.</p>
PDB files of "Characterization of ligand-induced thermal stability of the human organic cation transporter 2 (OCT2)"
<p>The predicted alphafold structure of OCT2 (Uniprot O15244) was embedded in a DPPC model membrane, solvated with TIP3P water and subjected to 250 ns molecular dynamics simulation using the Desmond module of Schroedingers Drug Discovery Suite. The last 150 ns were used for clustering and the cluster with most of the members (cluster0) was selected as a model for OCT2. Based on that (still predicted, but optimised) model we performed docking with various substrates for which we also provide the obtained docking pose. See Publication in Frontiers in Pharmacology (DOI: 10.3389/fphar.2023.1154213) for details and results.</p>
Dataset for "Characterization of rainwater infiltration within a controlled experiment by self-potential monitoring and modeling"
<p>This dataset provides the raw experimental data for the manuscript "Characterization of rainwater infiltration within a controlled experiment by self-potential monitoring and modeling". By imposing rainfall, we monitored the self-potential, volumetric water content, and temperature at different depths of a soil-column model within a water infiltration process. </p> <p> </p>
Nanoscale chemical characterization of secondary protein structure of F-Actin using mid-infrared photoinduced force microscopy (PiF-IR)
<p>Raw data for manuscript for special issue in Spectrochimica Acta related to ECSBM 2022</p> <p> </p>
Data of Forced-response characterization of PBF-LB/AlSi10Mg particle dampers with thin and flat cavities
<p>Powder Bed Fusion (PBF) enables the production of complex geometries which offer the opportunity to manufacture lightweight, stiffness-optimised or integrally designed components. Although these properties are usually advantageous for the performance in many applications, they pose disadvantages under vibration as they lead to low damped components. These are prone to high vibration amplitudes which result in higher sound radiation and a reduced lifetime. Particle damping can counteract these disadvantages. By including cavities during the design process, unmelted powder remains inside the component after its production. This powder dissipates energy under vibration by inelastic impacts and friction in particle-–particle or particle-–wall-interactions, increasing the damping characteristics of the component. In this work, additively manufactured AlSi10Mg specimens with cavities are investigated with respect to their damping characteristics by experimental modal analysis. The focus of the investigation is on thin and flat cavities that can be easily integrated into components without adapting the external geometry. The damping characteristics in dependence on excitation amplitude and mode are quantified. The extent to which settling effects of the powder during shaking influence the damping is analysed. The vibration of the specimens is forced by an electrodynamic shaker and their response is measured contactlessly via Scanning Laser Doppler Vibrometry (SLDV). A damping effect of up to 564% depending on the mode, excitation amplitude and specimen can be achieved. In addition, a significant settling effect of the powder which hampers the damping effect is identified by CT scans and modal analysis.<br> The data of the CT scans as well as the data of the modal analysis is uploaded here. </p>
Data and analysis result for "A scalable variational approach to characterize pleiotropic components across thousands of human diseases and complex traits using GWAS summary statistics"
<p>Data set and analysis results from our paper "A scalable variational approach to characterize pleiotropic components across thousands of human diseases and complex traits using GWAS summary statistics" (pre-print). This file contains GWAS summary statistics of 2,483 traits and 51,399 SNP variants from European individuals, originally downloaded and processed from Pan-UK Biobank (https://pan.ukbb.broadinstitute.org/). Additionally, we include results of 100 pleiotropic factors inferred by our method and tSVD as comparison. Please see README for detailed breakdown.</p>
Data and code for "Cell shape characterization, alignment and comparison using FlowShape"
<p><strong>Code</strong></p> <p>This folder contains a snapshot of the FlowShape Python package at the time of paper submission. The latest version is available at: <a href="https://bitbucket.org/pgmsembryogenesis/flowshape/src/main/">https://bitbucket.org/pgmsembryogenesis/flowshape/src/main/</a></p> <p><strong>Data</strong></p> <p>Contain 3D meshes of <em>C. elegans</em> early embryo cells, in Wavefront .obj format. Each filename has three parts sepparated by underscores</p> <ul> <li>Embryo identifier</li> <li>Cell name</li> <li>Timestep For example: <code>wt13_ABar_18</code> Embryo = wt13 Cell = ABar Timestep = 18</li> </ul> <p>The timesteps are frames from the original timelapse, where one frame corresponds to 90 seconds. The starting point is arbitrary, so time is aligned by EMS division.</p> <p>Embryo labels:</p> <ul> <li><code>7cell01-7cell07</code>: 7 wild-type embryos imaged only around the seven-cell stage.</li> <li><code>wt01-wt19</code>: 19 wild-type embryos imaged for a longer time.</li> <li><code>dshmig01-dshmig05</code>: 5 dsh-2 / mig-5 RNAi knockdown embryos, imaged around seven-cell stage.</li> </ul> <p><code>cell_filters.csv</code> is a CSV file containing identifiers of embryos left out of the analysis because of automatically detected errors. Further, wt06 was also left out because of problems with labeling the cells.</p> <p><strong>Images</strong></p> <p>Contains raw microscopy data that was used to generate the meshes. <code>resolution.csv</code> contains a list of Z, Y and X resolutions (micron / pixel).</p> <p><strong>Changelog</strong></p> <p>0.1.0</p> <ul> <li>Initial upload</li> </ul> <p>0.2.0</p> <ul> <li>Added microscopy images.</li> <li>Removed cells 6 and 7 from dsh-2 / mig-5 dataset, which were not supposed to be included there.</li> <li>Removed Python cache files.</li> </ul>
Genetic Characterization of the Norwegian Apple Collection
<p>Commercial fruit production in Norway is located at around latitude 60° north, demanding careful choice of adapted cultivars. The most comprehensive collection of apple genetic resources in Norway is being kept in the Norwegian Apple Collection (NAC) at Njøs Fruit and Berry Centre (NJØS). The collection contains around 350 accessions and was recently genotyped with a single nucleotide polymorphism (SNP) array. Curated SNP data were used for structure assessment, diversity analysis, pedigree confirmation, and core collection development. In the subsequent SNP analysis, we identified several duplicates and parent–offspring relationships. The collection was equally diverse across the geographic regions represented. Different methods for analyzing population structure were applied. K-means clustering and a Bayesian modeling approach with prior assumptions of the data revealed five subpopulations associated with geographic breeding centers. The collection has a distinct genetic structure and low relatedness among the accessions; hence, two 100 accession core collections were created. These new core collections will allow breeders and researchers to use the NAC efficiently. The results from this study suggest that several of the accessions in the Norwegian Apple Collection could be of high importance for breeding programs.</p>
FIG. 1 in Molecular characterization and morpho-taxonomy of Gambierdiscus caribaeus Vandersea, Litaker, M.A.Faust, Kibler, W.C.Holland & P.A.Tester (Dinophyceae) from Mauritius Island, South-West Indian Ocean
FIG. 1. — Map showing the three locations where samples were collected from Mauritius.
PW laser characterization
<p>Supplementary material of the thesis <em>“Development of ultra-stable characterization techniques for ultrashort laser beams”. </em>Spatiospectral characterization of the ATLAS-3000 laser relative to 800 nm, thus assuming flat phase at that wavelength. Measurement done outside the vacuum chamber using an achromatic imaging system to reduce the 20 cm beam diameter to mm-scale and characterized with a 3-mm-thick calcite cut with 45° optical axis angle. Spatiospectral intensity (a) and phase (b) profiles evolution.</p>
Model for: Characterizing long‐term population conditions of the elusive red tree vole with dynamic individual‐based modeling
<div class="abstract"> <p>Old growth forests are declining globally, threatening dependent wildlife. Many arboreal old‐growth obligates, such as the threatened red tree vole, are difficult to monitor for changes in habitat occupancy, and abundance. Yet, conservation planning relies on this information to prevent population declines. We integrated a range of species, habitat, and landscape change information to develop a dynamic habitat‐population model. The spatial individual‐based model simulated dynamic patterns of occupancy that responded to annual habitat maps, describing 36 years of observed change. We simulated population dynamics and local movement to characterize changes in occupancy and abundance, and the capacity of remaining habitat to support red tree voles. Red tree vole redistribution patterns strongly corresponded to wildfire footprints and timber extraction locations. Population strongholds are likely to exist in clumped pockets of old‐growth forest that were unaffected by wildfire and in protected old forest reserves. However, the exact number and locations of local clusters remain uncertain. Simulated population losses occurred at different paces in different places, underscoring the need for recurring evaluation of population changes with field occupancy surveys and modeled evaluations that can anticipate potential connectivity and extirpation thresholds. This modeling approach was effective at leveraging existing information for a data‐light species to assess how historical changes to the quantity, quality, and configuration of habitat likely influenced the potential landscape capacity, species abundance, and distribution. Dynamic individual‐based modeling can benefit conservation planning for red tree vole and other reclusive forest species by providing biologically nuanced assessments of abundance and distribution. Such models can also project the long‐term benefits and impacts of spatially explicit land management plans.</p> </div> <div class="abstract"></div>
Polymer characterization of submerged plastic litter from Lake Tahoe, United States
<p>Monitoring plastic litter in the environment is critical to understanding the amount, sources, transport, fate, and environmental impact of this pollutant. However, few studies have monitored plastic litter on lakebeds which are potentially important environments for determining the fate and transport of plastic litter in freshwater basins. In this study, self-contained underwater breathing apparatus (SCUBA) was used for litter collection at the lakebed along transects at five locations in Lake Tahoe, United States. Litter was brought to the surface and characterized by litter type. Plastic litter was subsampled for polymer determination using Attenuated Total Reflectance-Fourier Transform InfraRed (ATR-FTIR) spectroscopy. The average plastic litter recovered from the lakebed for the five dive sites was 83 ±49 items per kilometer. The top plastic litter categories were other plastic litter (i.e. plastic litter that did not fall in another category), followed by food containers, plastic bags, toys, and bottles <2 L. These results are in line with prior studies on submerged litter, supporting that intervention approaches or ongoing education is needed. The six polymers most frequently detected in the subsamples were polyvinyl chloride, polystyrene/expanded polystyrene, polyethylene terephthalate/polyester, polyethylene, polypropylene, and polyamide. These observations generally reflect the global plastic production trend and global compilations of microplastic studies from lake surface water and sediments. We found that some litter subcategories were primarily comprised of a single polymer type, therefore, in studies where the polymer type cannot be measured but litter is categorized, these results could provide an estimate of the total polymer composition for select subcategories.</p>
Characterization of the biochemical and behavioral effects of cannabidiol: implications for migraine
<p>This dataset comprises the findings obtained in the study aimed at investigating the anti-migraine effects of cannabidiol (CBD) in the migraine rat models based by nitroglycerin (NTG) administration. We assayed CBD distribution (15 and 30mg/kg, i.p.) in plasma and in cranial areas related to migraine pain in male Sprague Dawley rats treated chronically (5 days). Successively, we tested CBD activity on the behavioral and biochemical effects induced in the acute and the chronic migraine animal models by NTG administration. In the acute migraine model, rats received CBD (15 mg or 30 mg/kg, i.p) 3 h after NTG (10 mg/kg i.p.) or vehicle injection. In the chronic migraine model, rats were treated with CBD and NTG every other day over nine days with the following doses: CBD 30 mg/kg i.p., NTG 10 mg/kg i.p. We evaluated behavioral parameters with the open field and the orofacial formalin tests. We explored the fatty acid amide hydrolase (FAAH) gene expression, cytokines mRNA and protein levels in selected brain areas and CGRP serum level. CBD levels in the meninges, trigeminal ganglia, cervical spinal cord, medulla pons, and plasma were higher 1 h after the last treatment than after 24 h, suggesting that CBD penetrates but does not accumulate in these tissues. In the acute model, CBD significantly reduced NTG-induced trigeminal hyperalgesia and CGRP and cytokine mRNA levels in peripheral and central sites. In the chronic model, CBD caused only a significant decrease in NTG-induced IL-6 protein levels in the medulla-pons, and trigeminal ganglion. It also reduced CGRP serum levels. In both experimental conditions, there was no modulation of anxiety, motor/exploratory behavior, or grooming.</p> <p>The in vivo and ex vivo assessments were:</p> <p>1) mRNA expression levels: CGRP, SP, interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) and FAAH in cervical spinal cord, medulla-pons and trigeminal ganglia. mRNA levels were measured by rt-PCR. All samples were assayed in triplicate and gene expression levels were calculated according to 2−∆∆Ct = 2− (∆Ct gene − ∆Ct housekeeping gene) formula by using Ct (cycle threshold) values.</p> <p>2) Central and peripheral CBD levels were quantified in rat brain tissue and plasma samples using a previously proven online solid phase extraction (SPE) high-performance liquid chromatography (HPLC) method coupled with tandem mass spectrometry (MS/MS).</p> <p>3) Cytokines and CGRP protein levels were measured using the ELISA kits. The samples' measured absorbance was compared to a standard curve using a microplate reader.</p> <p>4) Behavior Formalin test= the face rubbing was measured counting the seconds the animal spent grooming the injected area (seconds).</p> <p>5) open field test= distance (expressed in meters) travelled in the apparatus; time spent (expressed in seconds) in the center of the apparatus; number of rearing; time spent in grooming behavior (expressed in seconds).</p> <p>Results in brief</p> <p>These data show that CBD modulates migraine-related nociceptive transmission, likely via a complex signaling mechanism involving different pathways.</p>
Topological characterization of the retinal microvascular network visualized by portable fundus camera- effects of chronic disease (TREND2) database
<p><strong>Introduction</strong></p> <p><strong>T</strong>opological characterization of the <strong>R</strong>etinal microvascular n<strong>E</strong>twork visualized by portable fu<strong>ND</strong>us camera (<strong>TREND 2</strong>) is a database of digital color eye fundus images created as an addition to TREND database (https://zenodo.org/badge/DOI/10.5281/zenodo.4521044.svg).</p> <p>TREND 2 databse was created by medical professionals of the Faculty of Medicine of the University of Montenegro in 2023.</p> <p> </p> <p><strong>Purpose</strong></p> <p>1) to provide a standard that defines normal and abnormal retinal anatomy and microvascular geometry as it appears when visualized by the portable fundus camera</p> <p>2) to help the development of new methods for stratification of the risk for the development of various eye diseases, as well as systemic diseases that affect microvasculature</p> <p>3) to aid the development of biomarkers of accelerated aging</p> <p>4) to provide a standard that can be used to develop software for segmentation of retinal microvasculature, grading the quality of retinal digital images, and computer-aided diagnosis of systemic and chronic diseases.</p> <p>All color digital images were acquired with a hand-held portable, non-mydriatic MiiS HORUS Scope DEC 200 with 45º FOV and 2560 X 1920 pixel resolution.</p> <p> </p> <p><strong>Data</strong></p> <p>The TREND public database contains 28 color fundus images of old subjects (20 images from subjects with one or more chronic diseases such as type 2 diabetes mellitus, hypertension or Alzheimer's dementia- O_CD group, and 8 images from subjects with no chronic diseases- O_NCD group). Each image is associated with a corresponding binarized image of the manually segmented microvascular network.</p> <table> <caption>Inclusion and Exclusion Criteria</caption> <thead> <tr> <th scope="col">O_NCD group</th> <th scope="col">O_CD group</th> </tr> </thead> <tbody> <tr> <td><strong>Inclusion Criteria</strong></td> <td><strong>Inclusion Criteria</strong></td> </tr> <tr> <td>- at least 56 years old</td> <td>- at least 56 years old</td> </tr> <tr> <td> <p>- no current acute disease</p> <p>- no history of alcohol, or drug abuse, or psychiatric disease</p> </td> <td> <p>- no current acute disease</p> <p>- no history of alcohol, or drug abuse, or psychiatric disease</p> </td> </tr> <tr> <td> <p>- no history of alcohol, or drug abuse, or psychiatric disease</p> </td> <td>- no history of alcohol, or drug abuse, or psychiatric disease</td> </tr> <tr> <td>- negative history of any chronic disease</td> <td> <p>- controlled hypertension (blood pressure<140/90 mmHg), and/or</p> <p>- controlled type 2 diabetes mellitus, and/or</p> <p>- Alzheimer's dementia</p> </td> </tr> <tr> <td><strong>Exclusion Criteria</strong></td> <td><strong>Exclusion Criteria</strong></td> </tr> <tr> <td> <p>- presence of opacities of the transparent media in both eyes affecting</p> <p>- myopia ≥5 diopters</p> </td> <td> <p>- presence of opacities of the transparent media in both eyes affecting</p> <p>- myopia ≥5 diopters</p> </td> </tr> </tbody> </table> <p><strong>Files:</strong></p> <p>1_OLD WITH CHRONIC DISEASE_RAW (20 images in tif format)</p> <p>2_OLD WITH CHRONIC DISEASE_SEGMENTED (20 images in png format)</p> <p>3_OLD WITH NO CHRONIC DISEASE_RAW (8 images in tif format)</p> <p>4_OLD WITH NO CHRONIC DISEASE SEGMENTED (8 images in png format)</p> <p>5_ASSOCIATED DATA (xslx format)</p> <p>6_RETINAL PATHOLOGY (docx format)</p> <p> </p> <p> </p>
Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy
<p><strong>This folder contains all raw data underlying the results presented in a manuscript, submitted for publication to <em>Methods and Applications in Fluorescence</em>, and entitled:</strong></p> <p> </p> <p><strong>Photo-physical characterization of high triplet yield brominated fluoresceins by transient state (TRAST) spectroscopy</strong></p> <p> </p> <p><strong>Authored by:</strong></p> <p>Baris Demirbay<sup>a</sup>, Glib Baryshnikov<sup>b</sup>, Martin Haraldsson<sup>c</sup>, Joachim Piguet<sup>a</sup>, Hans Ågren<sup>d</sup>, Jerker Widengren<sup>a,*</sup></p> <p> </p> <p><sup>a</sup>Royal Institute of Technology (KTH), Experimental Biomolecular Physics, Department of Applied Physics, Albanova University Center, SE-106 91, Stockholm, Sweden</p> <p><sup>b</sup>Laboratory of Organic Electronics, Department of Science and Technology, Linköping University, SE-60174, Norrköping, Sweden</p> <p><sup>c</sup>Chemical Biology Consortium Sweden, Science for Life Laboratory, Department of Medical Biochemistry and Biophysics, Karolinska Institute, SE-171 77 Stockholm, Sweden</p> <p><sup>d</sup>Department of Physics and Astronomy, Uppsala University, SE-751 20 Uppsala, Sweden</p> <p> </p> <p>*Corresponding Author:</p> <p> Email: <a href="mailto:jwideng@kth.se">jwideng@kth.se</a>, Phone: +46-8-7907813</p> <p> </p> <p><strong>The data files are grouped into the different techniques used to generate them, and refer to the figures/tables in the manuscript where the extracted results are presented.</strong></p> <p> </p> <p><strong>ABSTRACT</strong></p> <p>Photo-induced dark transient states of fluorophores can pose a problem in fluorescence spectroscopy, but their typically long lifetimes can also make them highly environment sensitive. This opens for exploiting these states as microenvironmental read-out parameters in bio-molecular spectroscopy and imaging, and to explore fluorophores with prominent dark-state formation yields to be used in such studies. In this work, we analyzed the singlet-triplet transitions of fluorescein by transient state (TRAST) spectroscopy and compared them with those of three synthesized carboxy-fluorescein derivatives, with one, two or four bromines linked to the anthracence backbone. By this bromination, a prominent internal heavy atom (IHA) enhancement of the intersystem crossing (ISC) rates was found, and a corresponding external heavy atom (EHA) enhancement upon adding potassium iodide (KI) into the fluorophore solutions. Notably, increased KI concentrations still resulted in lowered triplet state buildup in the brominated fluorophores, due to the relatively lower increase induced in the ISC, than in the triplet decay. Moreover, KI had an antioxidative effect on the fluorophores, resulting in an overall fluorescence enhancement of the brominated fluorophores. The mechanisms behind the prominent IHA effect were further investigated by density functional theory calculations, which suggest that the ISC likely takes place to a higher triplet state, followed by relaxation to the lowest triplet state.</p> <p>By TRAST measurements, performed under biologically relevant conditions and analyzing how the average fluorescence intensity of fluorescent molecules varies with a systematically varied excitation modulation, dark state transitions within very high triplet yield (>90%) fluorophores can be directly analyzed, as well as IHA and EHA effects. These measurements, not possible by other techniques such as fluorescence correlation spectroscopy, opens for bio-sensing applications based on high triplet yield fluorophores, and for characterization of high triplet yield photodynamic therapy agents, and how they are influenced by IHA and EHA effects. </p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.