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1,696 results for “DNA sequence”
Figure 1 from: Zhao L, Gao T, Lu W (2015) Complete mitochondrial DNA sequence of the endangered fish (Bahaba taipingensis): Mitogenome characterization and phylogenetic implications. ZooKeys 546: 181-195. https://doi.org/10.3897/zookeys.546.5964
Figure 1 - The structure of control region about Bahaba taipingensis.
Data from: Direct PCR meets high-throughput sequencing - metabarcoding of chironomid communities without DNA extraction
<p>Abstract</p> <p>Metabarcoding is a valuable tool for investigating insect community compositions. However, high-throughput applications, such as for biomonitoring, require cost-effective and user-friendly procedures. To investigate if the time-consuming and labour-intensive DNA isolation step can be omitted in metabarcoding, we studied the difference in detection rates and individual read abundance using standard DNA isolation versus direct PCR protocols. Metabarcoding with and without DNA isolation was performed on artificially created communities with known composition as well as on natural communities both of the dipteran family Chironomidae to compare detection rates, individual read abundances and presence-absence community composition. The data sets include read abundances of all artificial and natural community samples. Compositions of the samples per data set are described in the respective README files. ASVs/OTUs and their respective DNA sequences are given. R Scripts for bioinformatic processing (dada2 for ASVs, JAMP for OTUs) are provided.</p> <p>Methods</p> <p>Chironomidae were retrieved from artificial ponds of the Eußerthal Ecosystem Research Station (EERES) near Landau, Germany, in 2019 and 2020. Adult specimens were collected from passive emergence traps. Chironomid samples were stored in 70% ethanol and later dried at 60°C. Samples were then finely ground using a bead mill. PCR-grade water was added to each tissue sample and thoroughly vortexed. The tissue-water mixes were frozen at -20°C until further analysis. Artificial communities were created by pipetting tissue-water mixes of individual chironomids. Natural communities from eight ponds and five consecutive sampling dates were selected to assess the applicability of the dPCR approach compared to standard metabarcoding protocols on natural chironomid communities. Four of the artificial ponds were treated with the mosquito control agent <em>Bacillus thuringiensis israelensis</em> (Bti). Tissue-water mixes of artificial and natural communities were both directly applied to PCR and used for DNA isolation. Illumina sequencing was performed and raw data were bioinformatically prepared. For more details see "Direct PCR meets high-throughput sequencing - metabarcoding of chironomid communities without DNA extraction" (Röder & Schwenk 2023). Raw sequences are available through GenBank SRA archive (BioProject accession number PRJNA989176). </p>
Circulating Tumor DNA Sequencing in Patients With Peripheral T-cell Lymphomas
ClinicalTrials.gov study NCT06089941. IPD Sharing: NO. Countries: 1. Publications: 0.
Universal Endometrial Cancer DNA Sequencing for Detection of Lynch Syndrome and Personalized Care
ClinicalTrials.gov study NCT03460483. IPD Sharing: NO. Countries: 1. Publications: 0.
Sequencing-based Counting of Plasma Epstein-Barr Virus DNA in Non-metastatic Nasopharyngeal Carcinoma
ClinicalTrials.gov study NCT06321939. IPD Sharing: NO. Countries: 1. Publications: 0.
Whole Exome Sequencing in Finding Causative Variants in Germline DNA Samples From Patients With Hypertension Receiving Bevacizumab for Breast Cancer
ClinicalTrials.gov study NCT02610413. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Whole Exome Sequencing in Finding Causative Variants in Germline DNA Samples From Patients With Peripheral Neuropathy Receiving Paclitaxel for Breast Cancer
ClinicalTrials.gov study NCT02610439. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Development of a Tissue-Based & Cell Free DNA Next-Generation Sequencing Workflow
ClinicalTrials.gov study NCT02788084. IPD Sharing: NO. Countries: 1. Publications: 0.
DNA Sequencing-Based Monitoring of Minimal Residual Disease to Predict Clinical Relapse in Aggressive B-cell Non-Hodgkin Lymphomas
ClinicalTrials.gov study NCT02633111. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Implications of Maternal 45,X Mosaicism as a Secondary Genomic Finding Following Cell-Free DNA Sequencing During Pregnancy: A Deep Phenotype Study
ClinicalTrials.gov study NCT05548881. IPD Sharing: NO. Countries: 1. Publications: 0.
Optimization of HIV-1 DNA Genotyping by High Throughput Sequencing to Document Antiretroviral Resistant Mutations
ClinicalTrials.gov study NCT03512795. IPD Sharing: Not stated. Countries: 1. Publications: 0.
A Prospective, Multi-center Clinical Study to Establish Multi-Cancer Early Detection Platform Through the Analysis of Whole Genome Sequencing of Circulating DNA in Cancer Patients and Healthy Voluntee
ClinicalTrials.gov study NCT06231953. IPD Sharing: NO. Countries: 1. Publications: 0.
Comparison of Circulating Tumor DNA and Tumor Tissue DNA by Targeted Sequencing in Non-Small Cell Lung Cancer
ClinicalTrials.gov study NCT02645318. IPD Sharing: Not stated. Countries: 1. Publications: 0.
Feasibility Study of a Molecular Karyotype Using a Very High-throughput Sequencing Approach, the "Massive Parallel Sequencing" on Circulating Tumor DNA
ClinicalTrials.gov study NCT04104633. IPD Sharing: NO. Countries: 1. Publications: 0.
Next Generation DNA Sequencing in Patients With Idiopathic Male Infertility
ClinicalTrials.gov study NCT02021721. IPD Sharing: UNDECIDED. Countries: 1. Publications: 0.
Whole Exome Sequencing in Finding Causative Variants in Germline DNA Samples From Patients With Congestive Heart Failure Receiving Therapy for Breast Cancer
ClinicalTrials.gov study NCT02610426. IPD Sharing: Not stated. Countries: 1. Publications: 0.
DNA Exome Sequencing TCM for Sjögren's Syndrome
ClinicalTrials.gov study NCT03935373. IPD Sharing: NO. Countries: 1. Publications: 0.
DNA sequencing for Round 5 of the pHLA-A2 yeast display library selection for MA2 Fab
GEO Series GSE210479. Saccharomyces cerevisiae. 1 samples. Type: Other.
pooled clone sequencing from DNA cloning sample (ENCSR556IED)
GEO Series GSE266815. synthetic construct. 5 samples. Type: Other.
Quantitative sequencing of 5-formylcytosine in DNA at single-base resolution
GEO Series GSE56572. Mus musculus. 12 samples. Type: Methylation profiling by high throughput sequencing.
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International Brain Laboratory public data
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OpenNeuro
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