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139 results for “Chironomids”

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zenodo28/100

Supplementary material 1 from: Theissinger K, Kästel A, Elbrecht V, Makkonen J, Michiels S, Schmidt S, Allgeier S, Leese F, Brühl C (2018) Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wetlands. Metabarcoding and Metagenomics 2: e21060. https://doi.org/10.3897/mbmg.2.21060

We provide all information regarding the library preparation.

opencc-zeroFeb 2018View details →
zenodo28/100

Supplementary material 2 from: Theissinger K, Kästel A, Elbrecht V, Makkonen J, Michiels S, Schmidt S, Allgeier S, Leese F, Brühl C (2018) Using DNA metabarcoding for assessing chironomid diversity and community change in mosquito controlled temporary wetlands. Metabarcoding and Metagenomics 2: e21060. https://doi.org/10.3897/mbmg.2.21060

Pipeline used for bioinformatic processing of metabarcoding data in Theissinger et al.

opencc-zeroFeb 2018View details →
zenodo28/100

Fig. 1 in The Oriental Genus Shangomyia Saether & Wang (Chironomidae: Diptera): Immature Stages, Biology, Putative Relationships And The Evolution Of Wood Mining In Chironomid Larvae

Fig. 1. Shangomyia impectinata Saether & Wang, 1993, Larva; A. Antenna; B. Mentum, ventral; C, Ventromental plate (x1000 magnification); D. Mandible, inner teeth and mola; E. Labrum, semi-lateral view of rotated labrum; F. Dorsal head.

opencc-by-4.0Dec 2003View details →
zenodo28/100

Figure 4 from: Amora G, Hamada N, Fusari LM, Andrade-Souza V (2015) An Asiatic Chironomid in Brazil: morphology, DNA barcode and bionomics. ZooKeys 514: 129-144. https://doi.org/10.3897/zookeys.514.9925

Figure 4 - Frequency of occurrence of the ventral length of the cephalic capsule of a Brazilian Chironomus population (Diptera: Chironomidae) showing the four larval instars.

opencc-by-4.0Jul 2015View details →
zenodo28/100

Figure 2 from: Amora G, Hamada N, Fusari LM, Andrade-Souza V (2015) An Asiatic Chironomid in Brazil: morphology, DNA barcode and bionomics. ZooKeys 514: 129-144. https://doi.org/10.3897/zookeys.514.9925

Figure 2 - NJ tree based on the COI sequences of the mtDNA of Chironomus (Diptera: Chironomidae) species. The sequence of Lipiniella fujiprimus was used as the outgroup. Bootstrap values > 50% are shown on branches. Accession numbers and countries are provided beside the species names. Species flagged with an asterisk (*) are neotropical species. Brazilian Chironomus population: Chironomus sp1BRA; Chironomus sp2BRA; Chironomus sp3BRA

opencc-by-4.0Jul 2015View details →
zenodo28/100

Figure 1 from: Amora G, Hamada N, Fusari LM, Andrade-Souza V (2015) An Asiatic Chironomid in Brazil: morphology, DNA barcode and bionomics. ZooKeys 514: 129-144. https://doi.org/10.3897/zookeys.514.9925

Figure 1 - Adult male and pupae. Chironomus striatipennis, Indian population. A Wing D Hypopygium, dorsal view G Anal spur, dorsal view. Chironomus kiiensis, Japanese population B Wing E Hypopygium, dorsal view H Anal spur, dorsal view. Chironomus striatipennis, Brazilian population C Wing F Hypopygium, dorsal view I Anal spur, dorsal view. Scale bar: 500 µm (A, B, C, G, H, I); 200 µm (D, E, F).

opencc-by-4.0Jul 2015View details →
zenodo28/100

Figs 13, 14. Libanochlites neocomicus Brundin, 1976 in New chironomid flies in Early Cretaceous Lebanese amber (Diptera: Chironomidae)

Figs 13, 14. Libanochlites neocomicus Brundin, 1976, male, specimen 723: (13) wing, scale bar = 0.3 mm; (14) genitalia, scale bar = 0.1 mm.

opencc-by-4.0Apr 2007View details →
zenodo28/100

Figs 10–12 in New chironomid flies in Early Cretaceous Lebanese amber (Diptera: Chironomidae)

Figs 10–12. Lebanorthocladius furcatus gen. et sp. n., holotype 5B: (10) wing, scale bar = 0.5 mm; (11, 12) dorsal and ventral aspects of male genitalia, scale bar = 0.1 mm.

opencc-by-4.0Apr 2007View details →
zenodo28/100

Figs 7, 8 in New chironomid flies in Early Cretaceous Lebanese amber (Diptera: Chironomidae)

Figs 7, 8. Wadelius libanicus gen. et sp. n.: (7) wing, holotype 748A, scale bar = 0.5 mm; (8) male genitalia, paratype 259, scale bar = 0.1 mm.

opencc-by-4.0Apr 2007View details →
zenodo28/100

Figure 4 in Chironomids: A Personal Journey

Figure 4. Socorro Avila, parataxonomist, collecting larvae and pupal exuviae in the Sarapiquí River, April 2015.

opencc-by-4.0Dec 2015View details →
zenodo28/100

Supplementary material 1 from: Röder N, Schwenk K (2023) Direct PCR meets high-throughput sequencing – metabarcoding of chironomid communities without DNA extraction. Metabarcoding and Metagenomics 7: e102455. https://doi.org/10.3897/mbmg.7.102455

Overview of chironomid size classes

opencc-zeroJul 2023View details →
zenodo28/100

Supplementary material 2 from: Röder N, Schwenk K (2023) Direct PCR meets high-throughput sequencing – metabarcoding of chironomid communities without DNA extraction. Metabarcoding and Metagenomics 7: e102455. https://doi.org/10.3897/mbmg.7.102455

Composition of the two artificial chironomid communities

opencc-zeroJul 2023View details →
zenodo24/100

Figure 3 from: Amora G, Hamada N, Fusari LM, Andrade-Souza V (2015) An Asiatic Chironomid in Brazil: morphology, DNA barcode and bionomics. ZooKeys 514: 129-144. https://doi.org/10.3897/zookeys.514.9925

Figure 3 - Chironomus striatipennis, Brazilian population. A Egg mass B Egg.

opencc-by-4.0Jul 2015View details →
zenodo24/100

Data from: Direct PCR meets high-throughput sequencing - metabarcoding of chironomid communities without DNA extraction

<p>Abstract</p> <p>Metabarcoding is a valuable tool for investigating insect community compositions. However, high-throughput applications, such as for biomonitoring, require cost-effective and user-friendly procedures. To investigate if the time-consuming and labour-intensive DNA isolation step can be omitted in metabarcoding, we studied the difference in detection rates and individual read abundance using standard DNA isolation versus direct PCR protocols. Metabarcoding with and without DNA isolation was performed on artificially created communities with known composition as well as on natural communities both of the dipteran family Chironomidae to compare detection rates, individual read abundances and presence-absence community composition. The data sets include read abundances of all artificial and natural community samples. Compositions of the samples per data set are described in the respective README files. ASVs/OTUs and their respective DNA sequences are given. R Scripts for bioinformatic processing (dada2 for ASVs, JAMP for OTUs) are provided.</p> <p>Methods</p> <p>Chironomidae were retrieved from artificial ponds of the Eu&szlig;erthal Ecosystem Research Station (EERES) near Landau, Germany, in 2019 and 2020. Adult specimens were collected from passive emergence traps. Chironomid samples were stored in 70% ethanol and later dried at 60&deg;C. Samples were then finely ground using a bead mill. PCR-grade water was added to each tissue sample and thoroughly vortexed. The tissue-water mixes were frozen at -20&deg;C until further analysis. Artificial communities were created by pipetting tissue-water mixes of individual chironomids. Natural communities from eight ponds and five consecutive sampling dates were selected to assess the applicability of the dPCR approach compared to standard metabarcoding protocols on natural chironomid communities. Four of the artificial ponds were treated with the mosquito control agent <em>Bacillus thuringiensis israelensis</em> (Bti). Tissue-water mixes of artificial and natural communities were both directly applied to PCR and used for DNA isolation. Illumina sequencing was performed and raw data were bioinformatically prepared. For more details see &quot;Direct PCR meets high-throughput sequencing - metabarcoding of chironomid communities without DNA extraction&quot; (R&ouml;der &amp; Schwenk 2023). Raw sequences are available through GenBank SRA archive (BioProject accession number PRJNA989176). &nbsp;&nbsp;</p>

opencc-by-4.0Jun 2023View details →
geo20/100

Gene expression patterns and life cycle responses of toxicant exposed chironomids

GEO Series GSE40010. Chironomus riparius. 138 samples. Type: Expression profiling by array.

openGEO-OpenAug 2013View details →
zenodo20/100

FIGURES 4–5 in Pagastia (P.) donoliveri sp. nov. - a new Nearctic alpine stream chironomid species (Diptera: Chironomidae: Diamesinae) from the Beartooth Mountains, Wyoming U.S. A.

FIGURES 4–5. Pagastia (P.) donoliveri sp. nov., male. 4, hypopygium in dorsal view; 5, lateral aedeagal lobes. Scale bars: 50 µm.

opennotspecifiedMar 2020View details →
zenodo20/100

FIGURES 1–3 in Pagastia (P.) donoliveri sp. nov. - a new Nearctic alpine stream chironomid species (Diptera: Chironomidae: Diamesinae) from the Beartooth Mountains, Wyoming U.S. A.

FIGURES 1–3. Pagastia (P.) donoliveri sp. nov., male. 1, head in frontal view; 2, pronotum and mesonotum in dorsal view; 3, part of thorax in lateral view.

opennotspecifiedMar 2020View details →
zenodo20/100

FIGURES 19–25 in Two new chironomid species of the genus Pseudokiefferiella Zavřel (Diptera: Chironomidae: Diamesinae) from the Amur River basin of Russia

FIGURES 19–25. Adult male of Pseudokiefferiella silinka sp. nov. 19, 21, hypopygium in dorsal view; 20, gonocoxite and gonostylus in dorsal view; 22, basal plate of gonocoxite; 23, transverse sternapodeme and aedeagal lobes; 24–25, gonostylus. Scale bars: 50 μm.

opennotspecifiedAug 2023View details →
zenodo20/100

FIGURES 7–12 in Two new chironomid species of the genus Pseudokiefferiella Zavřel (Diptera: Chironomidae: Diamesinae) from the Amur River basin of Russia

FIGURES 7–12. Pupa of Pseudokiefferiella matafonovi sp. nov. 7–8, precorneals; 9, tergite IV; 10, tergite V; 11, tergites V–VI; 12, tergites VII–VIII and anal segment. Scale bars: 50 μm.

opennotspecifiedAug 2023View details →

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