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126
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ShareScore release 0.9.0
Dataset results
126 results for “Extraction Method”
Facilitating high throughput collections-based genomics: a comparison of DNA extraction and library build methods
<p>Dataset for submission: "Facilitating high throughput collections-based genomics: a comparison of DNA extraction and library build methods"</p>
Identification of extracellular miRNA in archived serum samples by next-generation sequencing from RNA extracted using multiple methods
GEO Series GSE80274. Homo sapiens. 37 samples. Type: Non-coding RNA profiling by high throughput sequencing.
RNA-seq analysis of pathogen and host in the early stages of Plague pulmonary infection using a novel dual RNA extraction method
GEO Series GSE181680. Yersinia pestis; Mus musculus. 17 samples. Type: Expression profiling by high throughput sequencing.
A tomato fruit RNA extraction method that isolates mRNAs encoding secreted and endomembrane associated proteins
GEO Series GSE17222. Solanum lycopersicum. 3 samples. Type: Expression profiling by array.
On-line Solid Phase Extraction High Performance Liquid Chromatography Method Coupled with Tandem Mass Spectrometry for the Therapeutic Monitoring of Cannabidiol and 7-Hydroxy-cannabidiol in Human Serum and Saliva
<p>This database includes the raw data linked with the paper “On-line Solid Phase Extraction High Performance Liquid Chromatography Method Coupled with Tandem Mass Spectrometry for the Therapeutic Monitoring of Cannabidiol and 7-Hydroxy-cannabidiol in Human Serum and Saliva".</p> <p>Cannabidiol is a novel antiseizure medication approved in Europe and the US for the treatment of seizures associated with Lennox-Gastaut syndrome, Dravet syndrome and tuberous sclerosis complex. We describe in this article a new and simple liquid chromatography-mass spectrometry method (LC-MS/MS) for the determination of cannabidiol and its active metabolite 7-hydroxy-cannabidiol in microvolumes of serum and saliva (50 μL), to be used as a tool for therapeutic drug monitoring (TDM) and pharmacokinetic studies. After on-line solid phase extraction cannabidiol, 7-hydroxy-cannabidiol and the internal standard cannabidiol-d3 are separated on a monolithic C18 column under gradient conditions. Calibration curves are linear within the validated concentration range (10-1000 ng/mL for cannabidiol and 5-500 ng/mL for 7-hydroxy-cannabidiol).</p> <p>The method is accurate (intraday and interday accuracy within 94-112% for cannabidiol, 91-109% for 7-hydroxy-cannabidiol), precise (intraday and interday precision <11.6% for cannabidiol and <11.7% for 7- hydroxy-cannabidiol) and sensitive, with a LOQ of 2.5 ng/mL for cannabidiol and 5 ng/mL for 7-hydroxy-cannabidiol. The stability of the analytes was confirmed under different storage conditions. Extraction recoveries were in the range of 81-129% for cannabidiol and 100-113% for 7-hydroxy-cannabidiol.</p> <p>The applicability of the method to TDM was demonstrated by analysis of human serum and saliva samples obtained from patients with epilepsy treated with cannabidiol.</p>
Comparison of two RNA extraction methods for the molecular detection of SARS-CoV-2 from nasopharyngeal swab samples
<p>The database includes the raw data of the article “Comparison of two RNA Extraction Methods for the Molecular Detection of SARS-CoV-2 from nasopharyngeal swab samples”.</p> <p>Aim of this work was to compare a two commercially available kit for viral RNA extraction from human swab samples that differ mainly for time consuming (9 min. vs 35 min.) in order to improve SARS-CoV-2 diagnostic workflow.</p> <p>The database contains the data obtained by the following evaluations:</p> <p>a) RNA analysis for the evaluation of quality and quantity by nanodrop instrument</p> <p>b) Real Time PCR analysis of SARS-CoV-2 genes and internal control.</p> <p>The two procedures evaluated gave similar analytical results in terms of extracted RNA analysis and Real Time PCR data for gene expression.</p>
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.