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777 results for “GFP”
Split-GFP data for experimentally addressed Chroma proteins
<p>Raw Nanopore sequencing read counts from FACS-sorted cell populations and enrichment score calculations. Bin scores were calculated for each protein as detailed in the Supplemental Methods section. [L1, L2, L3] refer to experimental replicate IDs, and [G0, G1, G2, G3] refer to sorting bin IDs. PRE refers to pre-sort populations. N is normalized read counts within each bin, an ENR is read enrichment for each bin compared to PRE. Bin scores were averaged for duplicate or triplicate biological replicate experiments, as indicated. Secondary structure information for each protein also included: G = 3-turn helix (310 helix). Min length 3 residues; H = 4-turn helix (α helix). Minimum length 4 residues; I = 5-turn helix (π helix). Minimum length 5 residues; T = hydrogen bonded turn (3, 4 or 5 turn); E = extended strand in parallel and/or anti-parallel β-sheet conformation. Min length 2 residues; B = residue in isolated β-bridge (single pair β-sheet hydrogen bond formation); b = also isolated bridge bend (the only non-hydrogen-bond based assignment); C = coil (residues which are not in any of the above conformations).</p>
Strausberg_Tribolium_LA-GFP_tailpole_run (Excerpt timepoints 291-340)
<p>Tribolium castaneum, life-actin GFP (transgenic), imaged in a custom dual-illumination light sheet microscope</p> <p>This is an excerpt of time points 291-340 showing a mitotic wave.</p> <p>Voxel size: 0.52 x 0.52 x 2.04 microns <br> Frame delay: 1 minute </p> <p><strong>Notes (whole data set)</strong></p> <p>sample author: Daniela Vorkel</p> <p>organism: Tribolium castaneum, life-actin GFP<br> sample count: 1 <br> <br> fluorescence: 488 (GFP)</p> <p>frames: 1361<br> acqu. time: 22h40m<br> size: 316.8 GB </p> <p>sample description: early embryo, mitotic waves + gastrulation, tailpole view</p> <p>experimental notes: optics prefused<br> </p> <p><strong>File descriptions</strong></p> <ul> <li>ClearControl meta header files: <ul> <li>C0opticsprefused.index.txt</li> <li>C0opticsprefused.metadata.txt</li> </ul> </li> <li>ClearControl program: <ul> <li>program.txt</li> </ul> </li> <li>Background subtracted / maximum projected thumbnails as video: <ul> <li>Strausberg_thumbnails_sb_text_291-340.gif</li> </ul> </li> <li>Singe time point 3D stack: <ul> <li>Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-291.tif</li> </ul> </li> <li>Image stacks with 10 time points each: <ul> <li>Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-291-300.tif</li> <li>Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-301-310.tif</li> <li>Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-311-320.tif</li> <li>Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-321-330.tif</li> <li>Strausberg_Tribolium_LA-GFP_tailpole_run-C0opticsprefused-331-340.tif</li> </ul> </li> </ul>
Data from: Fluorescent sperm in a transparent worm: validation of a GFP marker to study sexual selection
Background: Sexual selection has initially been thought to occur exclusively at the precopulatory stage in terms of contests among males and female mate choice, but research over the last four decades revealed that it often continues after copulation through sperm competition and cryptic female choice. However, studying these postcopulatory processes remains challenging because they occur internally and therefore are often difficult to observe. In the transparent free-living flatworm Macrostomum lignano, a recently established transgenic line that expresses green fluorescent protein (GFP) in all cell types, including sperm, offers a unique opportunity to non-invasively visualise and quantify the sperm of a GFP-expressing donor inside the reproductive tract of wild-type recipients in vivo. We here test several aspects of the reproductive performance of the transgenic individuals and the accuracy of the techniques involved in assessing the GFP-expressing worms and their sperm. We then show the usefulness of these methods in a study on sperm displacement. Results: GFP-expressing worms do not differ from wild-type worms in terms of morphology, mating rate and reproductive success. In addition, we show that the GFP signal is reliably and unequivocally expressed by all GFP-expressing individuals observed under epifluorescence illumination. However, the intensity of the GFP signal emitted by sperm of GFP expressing donors can vary (which we show to be at least in part due to sperm ageing) and the GFP marker is inherited according to Mendel's laws in most, but not all, of the individuals. Nevertheless, we argue these two issues can be addressed with an appropriate experimental design. Finally, we demonstrate the value of the GFP-techniques by comparing the number of GFP-expressing sperm in a wild-type recipient before and after mating with a competing sperm donor, providing clear experimental evidence for sperm displacement in M. lignano. This result suggests that sperm donors can displace previously stored sperm and replace it with their own. Conclusion: The availability of the GFP-techniques in a transparent organism provide unique opportunities to visualise and quantify internal processes in the female reproductive tract after mating, which opens new avenues in the study of sexual selection.
Live cell imaging data of IRE1a-GFP foci formation
<p>Live cell imaging of XBP1 mRNA and IRE1a-GFP expressing cells.</p>
Data from: Live imaging of symbiosis: spatiotemporal infection dynamics of GFP-labelled Burkholderia symbiont in the bean bug Riptortus pedestris
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Data from: Fluorescent sperm in a transparent worm: validation of a GFP marker to study sexual selection
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Do early life experiences predict variation in the general factor of personality (GFP)?
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RNA-seq profiling of the Tg(myo6b:GFP-2A-rpl10a-3xHA) zebrafish hair cell IP vs. whole fish IN
GEO Series GSE102861. Danio rerio. 7 samples. Type: Expression profiling by high throughput sequencing.
RNA-sequencing of GFP+ cells 2 days post-transduction of LSK cells with Scr or miR-99 KD lentiviral vectors
GEO Series GSE101902. Mus musculus. 5 samples. Type: Expression profiling by high throughput sequencing.
modENCODE_White Lab: genome-wide ChIP data of GFP from 10T-W3L on Illumina Genome Analyzer.
GEO Series GSE23124. Drosophila melanogaster. 3 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Peripherally derived macrophages can engraft the brain independent of irradiation and maintain an identity distinct from microglia [GFP]
GEO Series GSE108569. Mus musculus. 6 samples. Type: Expression profiling by high throughput sequencing.
modENCODE_White Lab: genome-wide ChIP data of GFP from E16-24h on Illumina Genome Analyzer.
GEO Series GSE25240. Drosophila melanogaster. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Using single-cell RNA-Seq for unbiased analysis of developmental hierarchies (single cell RNA Seq of Irf8-GFP GMP)
GEO Series GSE70241. Mus musculus. 37 samples. Type: Expression profiling by high throughput sequencing.
Identification of in vivo binding sites of human GFP-tagged C2H2-ZF proteins
GEO Series GSE76494. Homo sapiens. 160 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
modENCODE_White Lab: genome-wide ChIP data of NW-GFP-Goat[tll] from E0-4h on Illumina Genome Analyzer.
GEO Series GSE24136. Drosophila melanogaster. 3 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
Capture-C in somite-matched pre-somitic mesoderm from Smchd1GFP/GFP, Smchd1MD43-GFP/MD43-GFP, Smchd1+/+ and Smchd1MD43/MD43 embryos.
GEO Series GSE228673. Mus musculus. 13 samples. Type: Other.
Expression analysis of melanoma harvested after GFP or SETDB1 expression
GEO Series GSE26372. Danio rerio; Homo sapiens. 19 samples. Type: Expression profiling by array.
Gene expression signatures of MDCK-pTR GFP-RasV12 mCherry-β-catenin Δ131 cells cultured alone or mixed with MDCK mCherry-β-catenin Δ131 cells
GEO Series GSE217830. Canis lupus familiaris. 6 samples. Type: Expression profiling by array.
modENCODE_White Lab: genome-wide ChIP data of GFP from 5T-8-16h on Illumina Genome Analyzer.
GEO Series GSE25917. Drosophila melanogaster. 2 samples. Type: Genome binding/occupancy profiling by high throughput sequencing.
RNA changes in the cortex of 1-month old ICR mice overexpressing microRNA142-GFP compared to controls overexpressing tdtomato in the cortical neurons as determined by mRNA deep sequencing.
GEO Series GSE107167. Mus musculus. 10 samples. Type: Expression profiling by high throughput sequencing.
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Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.