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136 results for “Melanoplus”
FIGURE 4 in Two new species of Melanoplus Stål, 1873 (Orthoptera: Acrididae: Melanoplinae) from northeastern Mexico
FIGURE 4. Melanoplus sumichrasti sumichrasti, distal segments of male abdomen in dorsal view (a) and left lateral view (b).
FIGURE 3 in Two new species of Melanoplus Stål, 1873 (Orthoptera: Acrididae: Melanoplinae) from northeastern Mexico
FIGURE 3. Melanoplus bivittatus, distal segments of male abdomen in dorsal view (a) and left lateral view (b).
Supplementary material 1 from: Zembrzuski D, Woller DA, Jech L, Black LR, Reuter KC, Overson R, Cease A (2021) Establishing the nutritional landscape and macronutrient preferences of a major United States rangeland pest, Melanoplus sanguinipes, in field and lab populations. Journal of Orthoptera Research 30(2): 163-172. https://doi.org/10.3897/jor.30.61605
Supplementary material 1 from: Zembrzuski D, Woller DA, Jech L, Black LR, Reuter KC, Overson R, Cease A (2021) Establishing the nutritional landscape and macronutrient preferences of a major United States rangeland pest, Melanoplus sanguinipes, in field and lab populations. Journal of Orthoptera Research 30(2): 163-172. https://doi.org/10.3897/jor.30.61605
Data from: Combining RNA-seq and proteomic profiling to identify seminal fluid proteins in the migratory grasshopper Melanoplus sanguinipes (F)
Background: Seminal fluid proteins control many aspects of fertilization and in turn, they play a key role in post-mating sexual selection and possibly reproductive isolation. Because effective proteome profiling relies on the availability of high-quality DNA reference databases, our knowledge of these proteins is still largely limited to model organisms with ample genetic resources. New advances in sequencing technology allow for the rapid characterization of transcriptomes at low cost. By combining high throughput RNA-seq and shotgun proteomic profiling, we have characterized the seminal fluid proteins secreted by the primary male accessory gland of the migratory grasshopper (Melanoplus sanguinipes), one of the main agricultural pests in central North America. Results: Using RNA sequencing, we characterized the transcripts of ~ 8,100 genes expressed in the long hyaline tubules (LHT) of the accessory glands. Proteomic profiling identified 353 proteins expressed in the long hyaline tubules (LHT). Of special interest are seminal fluid proteins (SFPs), such as EJAC-SP, ACE and prostaglandin synthetases, which are known to regulate female oviposition in insects. Conclusions: Our study provides new insights into the proteomic components of male ejaculate in Orthopterans, and highlights several important patterns. First, the presence of proteins that lack predicted classical secretory tags in accessory gland proteomes is common in male accessory glands. Second, the products of a few highly expressed genes dominate the accessory gland secretions. Third, accessory gland transcriptomes are enriched for novel transcripts. Fourth, there is conservation of SFPs' functional classes across distantly related taxonomic groups with very different life histories, mating systems and sperm transferring mechanisms. The identified SFPs may serve as targets of future efforts to develop species- specific genetic control strategies.
FIGURES 10–11. Melanoplus ludivinae n in New Acrididae from Oaxaca State in Mexico (Orthoptera: Caelifera: Acrididae Ommatolampinae, Melanoplinae)
FIGURES 10–11. Melanoplus ludivinae n. sp., live specimens in nature. 10: male; 11: female.
FIGURES 8–9. Melanoplus oaxacae n in New Acrididae from Oaxaca State in Mexico (Orthoptera: Caelifera: Acrididae Ommatolampinae, Melanoplinae)
FIGURES 8–9. Melanoplus oaxacae n. sp., live specimens in nature. 8: male; 9: female.
FIGURE 33. Melanoplus parvus n in Two new species of Melanoplus Stål, 1873 (Orthoptera: Acrididae: Melanoplinae) from northeastern Mexico
FIGURE 33. Melanoplus parvus n. sp., habitat. Los Lirios, Arteaga, Coahuila, Mexico
FIGURE 26 in Two new species of Melanoplus Stål, 1873 (Orthoptera: Acrididae: Melanoplinae) from northeastern Mexico
FIGURE 26. Melanoplus reflexus, female's cercus and ovipositor's valvae.
FIGURE 23. Melanoplus trachodes n in Two new species of Melanoplus Stål, 1873 (Orthoptera: Acrididae: Melanoplinae) from northeastern Mexico
FIGURE 23. Melanoplus trachodes n. sp., living female.
FIGURE 21. Melanoplus trachodes n in Two new species of Melanoplus Stål, 1873 (Orthoptera: Acrididae: Melanoplinae) from northeastern Mexico
FIGURE 21. Melanoplus trachodes n. sp., habitat Miquihuana, Tamaulipas, Mexico.
FIGURE 20. Melanoplus trachodes n in Two new species of Melanoplus Stål, 1873 (Orthoptera: Acrididae: Melanoplinae) from northeastern Mexico
FIGURE 20. Melanoplus trachodes n. sp., living male.
FIGURE 22. Melanoplus trachodes n in Two new species of Melanoplus Stål, 1873 (Orthoptera: Acrididae: Melanoplinae) from northeastern Mexico
FIGURE 22. Melanoplus trachodes n. sp., living female.
Figure 3 from: Zembrzuski D, Woller DA, Jech L, Black LR, Reuter KC, Overson R, Cease A (2021) Establishing the nutritional landscape and macronutrient preferences of a major United States rangeland pest, Melanoplus sanguinipes, in field and lab populations. Journal of Orthoptera Research 30(2): 163-172. https://doi.org/10.3897/jor.30.61605
Figure 3 Performance experiments. Survival and specific growth rates of grasshoppers from the long-term lab colony no-choice diet experiments. A. The specific growth rates for each diet treatment. Diamonds indicate the mean and bolded lines indicate the median. Boxes are +/- 25%, lines represent minimum and maximum values excluding extreme values, and dots indicate data points > 1.5 farther from the box edge than the interquartile range. Lower case letters indicate differences from Mann-Whitney post-hoc analyses. B. The proportion of grasshoppers surviving through time on each diet treatment. Most diet treatments did not have individuals die until the 5th day of the experiment, and most treatments except 7p:35c had minimal deaths (although there were no significant differences among treatments). C. Proportion of grasshoppers molting to adults over time. Most of the diets saw increases in molting from days 5–7, except diet treatment 7p:35c, which was delayed and had the least number of grasshoppers successfully molt (significantly different from all other treatments).
Figure 2 from: Zembrzuski D, Woller DA, Jech L, Black LR, Reuter KC, Overson R, Cease A (2021) Establishing the nutritional landscape and macronutrient preferences of a major United States rangeland pest, Melanoplus sanguinipes, in field and lab populations. Journal of Orthoptera Research 30(2): 163-172. https://doi.org/10.3897/jor.30.61605
Figure 2 Field IT compared to nutritional landscape. A, B. Grasshopper intake targets of the field populations (black solid line) alongside the nutrient contents of grasses (triangles) and forbs (circles) collected from the same fields. The grey solid line represents the intake target from the other field population. The dotted line represents a 1p:1c ratio. C, D. The average Euclidean distance between the plants (triangles and circles in A and B) and either the grasshopper IT from each location or the 1p:1c line. * denotes a significant difference between the Euclidean distances calculated from the IT and the 1p:1c line.
Figure 1 from: Zembrzuski D, Woller DA, Jech L, Black LR, Reuter KC, Overson R, Cease A (2021) Establishing the nutritional landscape and macronutrient preferences of a major United States rangeland pest, Melanoplus sanguinipes, in field and lab populations. Journal of Orthoptera Research 30(2): 163-172. https://doi.org/10.3897/jor.30.61605
Figure 1 Field populations and lab population ITs. Average intake target (+/- SEM) for two field populations, Bliss and Boise, ID, and the lab colony. The dashed line represents a 1:1 ratio of protein and carbohydrates, and the crosses on the data points represent SE.
Data from: Combining RNA-seq and proteomic profiling to identify seminal fluid proteins in the migratory grasshopper Melanoplus sanguinipes (F)
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