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Fig. 7 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 7. Relative expression analysis of LoTPS2 and LoTPS4 during different flower development performed by quantitative real-time PCR. (a, b) Transcript levels of the LoTPS2 and LoTPS4 genes during flower development. (c, d) Relative expression analysis of LoTPS2 and LoTPS4 at different flower developmental stages. Flower development was divided into 5 different stages: D1 (bud stage), D2 (little open), D3 (half open), D4 (full-bloom), D5 (senescence). GAPDH was used as an internal control. The root was set as 1. Data are presented as the mean ± SEM (n = 3).

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Fig. 8 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 8. Relative expression analysis of LoTPS genes and the emission of (E, E)-α-farnesene and D-limonene from Lilium 'Siberia' at different time intervals. (a, b) Expression pattern of LoTPS2 and LoTPS4 for 3 days postanthesis. (c) Emission pattern of (E, E)-α-farnesene from Lilium 'Siberia' (d) Emission pattern of D-limonene from Lilium 'Siberia' flowers over 3 days after full-bloom. The plants were kept under a 12 h light, 12 h dark photoperiod. GAPDH was used as an internal control. Each point is the average of 3 replicates.

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Fig. 6 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 6. (a) A labeled diagram of a fullbloom Lilium 'Siberia' flower. (b) Pictorial view of Lilium 'Siberia' flowers at different flower developmental stages.

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Fig. 5 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 5. Relative gene expression analysis of LoTPS2 and LoTPS4 in floraland vegetative tissues of Lilium 'Siberia' (a) relative expression levels of LoTPS2 in different tissues of Lilium 'Siberia' (b) Relative expression levels of LoTPS4 in different tissues of Lilium 'Siberia' analyzed by qRT-PCR. (c, d) Relative expression levels of LoTPS2 and LoTPS4 in full-bloom flowers of different Lilium species. GAPDH was used as an internal control. The highest expression level was set as 1 (100%). Lon: Longiflorum; Bru: Brunello; Sib: Siberia; Aca: Acapulco; Sor: Sorbonne; Man: Manissa. Data are presented as the mean ± SEM (n = 4).

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Fig. 1 in Molecular cloning, characterization and expression analysis of LoTPS2 and LoTPS4 involved in floral scent formation in oriental hybrid Lilium variety 'Siberia'

Fig. 1. (a) Alignment of the amino acid sequences of LoTPS2 and LoTPS4, with AdAFS1 from Actinidia deliciosa (FJ265785) and (+)-limonene synthase (Q8L5K3) from Citrus limon. The protein sequences were aligned using ClustalX 2.1 and edited with GeneDoc. RRX8W motifs were present in LoTPS4 and (+)-limonene synthase but were missing in both LoTPS2 and AdAFS1. The conserved RRX8W, DDXXD, and RxR domains are underlined. (b) N-terminal sequence alignment of TPS-f clade terpene synthases. AdAFS1; CbLIS2 (C. breweri, AAD19840); CbLIS (C. breweri, AAC49395); CcLIS (Clarkia concinna, AAD19839). The CDIS (conifer diterpene internal sequence) is indicated by a dotted line. Dashes indicate gaps inserted for optimal alignment. (c) Phylogenetic analysis of LoTPS2 and LoTPS4 from Lilium 'Siberia' with amino acid sequences of other selected terpene synthases. The alignment was performed using ClustalX 2.1, and the tree was built via the neighborjoining method using the MEGA 6 program and iTOL (http://itol.embl.de/). The dot size at the branches of the tree shows bootstrap values. Accession numbers are given in a Supplementary Table 1.

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Fig. 6. A in Molecular cloning and functional characterization of a two highly stereoselective borneol dehydrogenases from Salvia officinalis L

Fig. 6. A) Chemical reduction of racemic camphor leads to a mixture of four stereoisomers, from which SoBDH2 oxidizes three; B) Chemical reduction of (+)-camphor produces a mixture of (+)-1 (20%) and (−)-2 (80%), from which SoBDH1 oxidizes (+)-1 and leaves (−)-2 unreacted.