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Figs 29‒32. Living individuals and habitats. 29 in Two new species of the genus Cryptostemma from Japan (Hemiptera: Heteroptera: Dipsocoridae)
Figs 29‒32. Living individuals and habitats. 29 – Cryptostemma miyamotoi sp. nov., male; 30 – C. pavelstysi sp. nov., male; 31 – habitat of C. miyamotoi, Sonosegawa Riv., Sanagouchi-son, Tokushima Pref.; 32 – habitat of C. pavelstysi, near Nagura Dam, Ishigaki Is.
Data for: Scalable and Live Trace Processing with Kieker Utilizing Cloud Computing
<p>Knowledge of the internal behavior of applications often gets lost over the years. This circumstance can arise, for example, from missing documentation. Application-level monitoring, e.g., provided by Kieker, can help with the comprehension of such internal behavior. However, it can have large impact on the performance of the monitored system. High-throughput processing of traces is required by projects where millions of events per second must be processed live. In the cloud, such processing requires scaling by the number of instances.</p> <p>In this paper, we present our performance tunings conducted on the basis of the Kieker monitoring framework to support high-throughput and live analysis of application-level traces. Furthermore, we illustrate how our tuned version of Kieker can be used to provide scalable trace processing in the cloud.</p> <p>This is the dataset containing the results of our conducted benchmarks.</p>
Vagrant Lives: 14,789 Vagrants Processed by Middlesex County, 1777-1786
<p><em><strong>This is no longer the most up to date version of this dataset. Please use version 1.1 (https://zenodo.org/record/31026) instead.</strong></em></p> <p>This dataset makes accessible the uniquely comprehensive records of vagrant removal from, through, and back to Middlesex, encompassing the details of some 14,789 men and women removed (either forcibly or voluntarily) as undesirables between 1777 and 1786. In includes people ejected from London as vagrants, and those sent back to London from counties beyond. Significant background material is available on the 'London Lives' website, which provides additional context for these records. The authors also recommend the following article:</p> <p> Tim Hitchcock, Adam Crymble, and Louise Falcini, ‘Loose, Idle and Disorderly: Vagrant Removal in Late Eighteenth-Century Middlesex’, _Social History_.</p> <p>Each record includes details on the name of the vagrant, his or her parish of legal settlement, where they were picked up by the vagrant contractor, where they were dropped off, as well as the name of the magistrate who had proclaimed them a vagrant. Each entry is georeferenced, to make it possible to follow the journeys of thousands of failed migrants and temporary Londoners back to their place of origin in the late eighteenth century.</p> <p>Each entry has 29 columns of data, all of which are described in the READ ME file.</p> <p>The original records were created by Henry Adams, the vagrant contractor of Middlesex who had - as had his father before him - conveyed vagrants from Middlesex gaols to the edge of the county where they would be sent onwards towards their parish of legal settlement. His role also involved picking up vagrants on their way back to Middlesex, expelled from elsewhere, as well as those being shepherded through to counties beyond, as part of the national network of removal. Eight times per year at each session of the Middlesex Bench, Adams submitted lists of vagrants conveyed as proof of his having transported these individuals, after which he would be paid for his services. The dataset contains all 42 surviving lists out of a possible 65.The gaps in the records are unfortunately not evenly spaced throughout the year. We know more, for example, about removal in October than in May.</p> <p>Spellings have been interpreted and standardized when possible. Georeferences have been added when they could be identified. This dataset was created for 21st century historians, and should not be construed as a true transcription of the original sources. Instead the goal was to use a limited vocabulary and to interpret the entries rather than recreate them verbatim. While this is undesirable for anyone interested in spelling variations of names and place names in the eighteenth century, it is the authors' hope that these interpretations will make it easier to conduct quantitative analysis and studies in historical geography.</p>
Supplementary Material: Fluorescent Protein‐Tagged Sindbis Virus E2 Glycoprotein Allows Single Particle Analysis of Virus Budding from Live Cells
<p>Supplementary Videos for <em>Viruses</em> <strong>2015</strong>, <em>7</em>(12), 6182-6199; doi:10.3390/v7122926, http://www.mdpi.com/1999-4915/7/12/2926:</p> <p><strong>Video S1A</strong> BHK cells infected with mCherry-E2 virus at 3 h p.i. Glycoprotein containing vesicles are transported to the PM from where individual virions bud out. White arrow point to budding virions. Overall amount of glycoproteins present on the PM and the number of virus particles budding out are relatively reduced compared to the late stage of infection. Images were acquired at a rate of 0.99 fps and 75 frames were acquired. Video was generated using these images and played at a rate of 5 fps. Image acquisition time is shown as Time: hour: minute: second: millisecond (h:min:sec:msec ) and the scale bar represents 10 μm.</p> <p><strong>Video S1B </strong>Enlarged area of video S1A showing budding virus particles from PM. White arrow indicates single particle post-budding moving away from the cell. Images were acquired at a rate of 0.99 fps and 75 frames were acquired. Video was generated using these images and played at a rate of 5 fps. Image acquisition time is shown as Time: hour: minute: second: millisecond (h:min:sec:msec ) and the scale bar represents 10 μm.</p> <p><strong>Video S2A</strong> Virus budding and single particle movement associated with filopodial extensions observed from mCherry-E2 virus-infected BHK cells at 6 h p.i. Glycoprotein containing vesicle transport to the PM is also observed. Budded virions travel along the periphery of filopodia and are released from filopodial extensions to the surrounding media. Image acquisition was at a rate of 1 fps and 285 frames were acquired. Movie was generated using these images and played at a rate of 7 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p><strong>Video S2B</strong> Enlarged area of video S2A showing budding virus particles from filopodia. White arrow indicates virus budding from filopodial extensions. Images were acquired at a rate of 1 fps and the acquired 285 frames were used to generate the video at a rate of 7 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p><strong>Video S3</strong> BHK cells transfected with RNA from a non-budding cdE2 mutant <sub>416</sub>CC<sub>417</sub>/A2 mCherry-E2 virus. This non-budding mutant is unable to release fluorescent virus particles from the infected cells. The video shows the absence of fluorescent virus particle budding from the PM at 6 h post transfection even though the PM and filopodial extensions contain mCherry-E2. Despite the transport of glycoproteins to the PM, no fluorescent particles were released into the media. Yellow arrows point toward filopodial extensions. For the video, 304 images were acquired at a rate of 0.98 fps and the video was generated using the acquired images at a rate of 7 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p><strong>Video S4</strong> BHK cells transfected with RNA from an E1 Fusion loop (G91D) mutant of mCherry-E2 virus at 6 h post transfection. This non-fusing mutant produces fluorescent virus particles at a slower rate compared to WT that are unable to fuse after entering a new cell. White arrow points to fluorescent particles that are releasing into the media from filopodial extensions. Yellow arrow represents a fluorescent particle that had entered an adjacent un-transfected cell. A total of 149 images were acquired at a rate of 0.98 fps. Video was generated using these images at a rate of 7 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p> </p> <p><strong>Video S5A</strong> Glycoprotein E2 (mCherry-E2; red) colocalizing with Golgi stain (green) in BHK cells infected with mCherry-E2 virus and stained with BODIPY FL C5 ceramide at 5 h p.i. and imaged at 6 h p.i. Glycoprotein-containing red vesicles originate from Golgi as evidenced from the colocalization of red and green and these vesicles display anterograde transport to the PM and the virus particles are released by budding from the PM. Fluorescent particles are also seen budding from filopodial extensions (white arrows). Images were acquired at a rate of 0.13 fps for 295 seconds. Video was generated using these acquired images at a rate of 5 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p><strong>Video S5B</strong> An enlarged area of the video S5A near the white arrow showing movement of particles on filopodial extensions between two cells. Movie was played at a rate of 5 fps. Image acquisition time is shown as Time: h:min:sec:msec and the scale bar represents 10 μm.</p> <p> </p> <p> </p>
The London Lives Petitions Project, v2.0
<p>This update adds data about identifiable petitioners, derived from London Lives name tagging, and slightly improves tags for churchwardens' (parish) petitions.</p>
Fig. 2. – Living plants. A in A synoptic revision of the Malagasy endemic genus Socratina Balle (Loranthaceae). Candollea 69: 65-73. In English, English and French abstracts.
Fig. 2. – Living plants. A. Socratina keraudreniana Balle; B. S. bemarivensis (Lecomte) Balle; C. S. phillipsoniana Callm. & Luino. [Photos: A: J. Bosser; B: F. Ratovoson; C: I. Luino]
Research data supporting "Online quantitative monitoring of live cell engineered cartilage growth using diffuse fiber-optic Raman spectroscopy"
<p>Research data supporting the publication:</p> <p>M. Bergholt, 2017, Online quantitative monitoring of live cell engineered cartilage growth using diffuse fiber-optic Raman spectroscopy, Biomaterials, Volume 140, September 2017, Pages 128–137, DOI: 10.1016/j.biomaterials.2017.06.015</p>
Figs 50–53. Live specimens. 50–52 in The Philippine hair wax spiders and their relatives: revision of the Pholcus bicornutus species group (Araneae, Pholcidae)
Figs 50–53. Live specimens. 50–52. Pholcus baguio Huber, sp. nov., ♁, ♀, and ♀ with egg-sac and emerging juveniles, Mt. Kabuyao. 53. Pholcus arayat Huber, 2011, ♁, Pamulaklakin Forest Trail.
Figs 54–57. Live specimens. 54. Pholcus arayat Huber, 2011 in The Philippine hair wax spiders and their relatives: revision of the Pholcus bicornutus species group (Araneae, Pholcidae)
Figs 54–57. Live specimens. 54. Pholcus arayat Huber, 2011, ♀, Pamulaklakin Forest Trail. 55–57. Pholcus pagbilao Huber, 2011, ♁ from Loboc (55), ♁ from Mt. Banahaw (56), and ♀ with egg-sac from Loboc (57).
Figs 36–39. Live specimens. 36–37 in The Philippine hair wax spiders and their relatives: revision of the Pholcus bicornutus species group (Araneae, Pholcidae)
Figs 36–39. Live specimens. 36–37. Pholcus mulu Huber, sp. nov., ♁, Gunung Mulu. 38–39. Pholcus kawit Huber, sp. nov., ♁ and ♀, Mt. Matutum.
Fig. 2. Guaranita Huber, 2000, live specimens. A–B. G in Revision of the South American Ninetinae genus Guaranita (Araneae, Pholcidae)
Fig. 2. Guaranita Huber, 2000, live specimens. A–B. G. dobby Torres et al., 2016; females with egg-sacs from NW of Campo Quijano. C–D. G. munda (Gertsch, 1982); male and female with eggsac from E of Nono. E–F. G. yaculica Huber, 2000; male and female from Calilegua National Park. G–H. G. auadae Huber sp. nov.; male and female with egg-sac from between San Salvador and Purmamarca. I–J. G. goloboffi Huber, 2000; male and female from NW of Chumbicha.
Fig. 13 in Madagascar's living giants: discovery of five new species of endemic giant pill-millipedes from Madagascar (Diplopoda: Sphaerotheriida: Arthrosphaeridae: Zoosphaerium)
Fig. 13. Zoosphaerium darthvaderi sp. n., holotype, left antenna, SEM: (A) lateral view, (B) apical view on disc.
Fig. 11 in Madagascar's living giants: discovery of five new species of endemic giant pill-millipedes from Madagascar (Diplopoda: Sphaerotheriida: Arthrosphaeridae: Zoosphaerium)
Fig. 11. Zoosphaerium darthvaderi sp. n., holotype: (A) habitus, lateral view; (B) coxa and prefemur of first left leg with first stigmatic plate, posterior view; (C) 9th left leg, posterior view; (D) anal shield, lateral view; (E) anal shield, dorsal view; (F) male second right coxa and prefemur with strongly sclerotised gonopore, posterior view. Roman numerals refer to number of tergite. Abbreviations: c – collum (tergite 1), h – head, AS – anal shield, Cx – coxa, Go – gonopore, Pre – prefemur, St – stigmatic plate, th-s – thoracic shield (tergite 2). Scale bars: (A) = 10 mm, (B–F) = 1 mm.
Fig. 17 in Madagascar's living giants: discovery of five new species of endemic giant pill-millipedes from Madagascar (Diplopoda: Sphaerotheriida: Arthrosphaeridae: Zoosphaerium)
Fig. 17. Endoterga of tergites 10, arrows point to cuticular impressions: (A) holotype of Zoosphaerium muscorum sp. n., (B) holotype of Z. bambusoides sp. n., (C) holotype of Z. tigrioculatum sp. n., (D) holotype of Z. darthvaderi sp. n., (E) holotype of Z. heleios sp. n. Abbreviations: ci – cuticular impressions, IA – inner area with spines and long setae, mr – marginal ridge, mb – marginal bristles.
ThermoCyte: an inexpensive open-source temperature control system for in vitro live cell imaging
<p>Live-cell imaging is a common technique in microscopy to investigate dynamic cellular behaviour and permits the accurate and relevant analysis of a wide range of cellular and tissue parameters, such as motility, cell division, wound healing responses, and calcium (Ca2+) signalling in cell lines, primary cell cultures, and ex vivo preparations. Furthermore, this can take place under many experimental conditions, making live-cell imaging indispensable for biological research. Systems which maintain cells at physiological conditions outside of a CO<sub>2</sub> incubator are often bulky, expensive, and use proprietary components. Here we present an inexpensive, open-source temperature control system for in vitro live cell imaging. Our system 'ThermoCyte', which is constructed from standard electronic components, enables precise tuning, control, and logging of a temperature 'set point' for imaging cells at physiological temperature. We achieved stable thermal dynamics, with reliable temperature cycling and a standard deviation of 0.42°C over 1 hour. Furthermore, the device is modular in nature, and is adaptable to the researcher's specific needs. This represents simple, inexpensive, and reliable tool for laboratories to carry out custom live-cell imaging protocols, on a standard lab bench, at physiological temperature.</p>
Figure 8. Living animals. A, B, Berghia verruciconnis from Huelva. C, Berghia columbina from Huelva. D, E, Spurilla neapolitana from Huelva. F in The family Aeolidiidae Gray, 1827 (Gastropoda Opisthobranchia) from Brazil, with a description of a new species belonging to the genus Berghia Trinchese, 1877
Figure 8. Living animals. A, B, Berghia verruciconnis from Huelva. C, Berghia columbina from Huelva. D, E, Spurilla neapolitana from Huelva. F, Spurilla neapolitana from El Grove.
Figure 1. Living animals. A, Anteaeolidiella indica from Praia dos Ossos. B in The family Aeolidiidae Gray, 1827 (Gastropoda Opisthobranchia) from Brazil, with a description of a new species belonging to the genus Berghia Trinchese, 1877
Figure 1. Living animals. A, Anteaeolidiella indica from Praia dos Ossos. B, Berghia benteva from Praia de Armação (10-mm-long specimen). C, D, Berghia benteva from Praia dos Ossos (18- and 19-mm-long specimens). E, Berghia creutzbergi from Praia de Armação (13-mm-long specimen). F, Berghia marcusi sp. nov., from Praia de Armação (dorsal view, 12-mm-long specimen). G, Berghia marcusi sp. nov. (ventral view of the same specimen). H–J, Spurilla neapolitana from Praia de Armação. K, Spurilla neapolitana from Praia dos Ossos.
Figure 34. Living animals. A in A phylogenetic analysis and systematic revision of the cryptobranch dorids (Mollusca, Nudibranchia, Anthobranchia)
Figure 34. Living animals. A, Paradoris indecora, Southern Spain, photo by D. Moreno. B, Otinodoris sp. (CASIZ 073238), photo by T. M. Gosliner. C, Sebadoris nubilosa, Seychelles, photo by T. M. Gosliner. D, Conualevia marcusi, La Paz Bay, Baja California Sur.
Figure 4. Living animals. A in A phylogenetic analysis and systematic revision of the cryptobranch dorids (Mollusca, Nudibranchia, Anthobranchia)
Figure 4. Living animals. A, Doris pseudoargus (CASIZ 121105). B, Doris immonda (CASIZ 089023), photo by T. M. Gosliner. C, Doris granulosa (CASIZ 073536), photo by T. M. Gosliner. D, Discodoris boholiensis (CASIZ 083654), photo by T. M. Gosliner. E, Discodoris ketos, San Pedrillo, Puntarenas, Costa Rica, photo by T. M. Gosliner. F, Thordisa rubescens (CASIZ 015860), photo by T. M. Gosliner. G, Aphelodoris antillensis (CASIZ 077289), photo by T. M. Gosliner. H, Peltodoris atromaculata (CASIZ 119474). I, Peltodoris nobilis, Monterey Bay, California, photo by A. Smith.
Figs 1–9 in Two new species of free-living nematodes (Nematoda) from Vietnam
Figs 1–9. Morphology of Monhistera vietnamica sp. nov. (1–5) and Brevitobrilus larae sp. nov. (6–9). 1, 6, entire body of female; 2, 7, head; 3, esophagus; 4, 8, vulval section; 5, 9, tail. Scale bars: 2, 7 – 5 µm; 3, 4, 5, 8, 9 – 20 µm; 1, 6 – 50 µm.
ScienceDex guides
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These curated guides explain access requirements, typical timelines, costs, and reuse considerations for widely used research datasets.
Allen Brain Atlas
Allen Brain Atlas is an Allen Institute collection of brain map atlases, datasets, APIs, and analysis tools covering mouse, human, and non-human primate brain resources.
Annotated Behaviour and Observability Dataset (ABODe)
ABODe is a University of Edinburgh DataShare dataset for behavior classification in group-housed mice using home-cage video, identities, bounding boxes, ground-plate positions, and annotator labels.
DANDI Archive for NWB datasets
DANDI is a BRAIN Initiative archive for publishing and sharing neurophysiology data, including electrophysiology, optophysiology, and behavioral data packaged as NWB and related standards.
International Brain Laboratory public data
The International Brain Laboratory public data releases expose standardized mouse decision-making experiments, including Neuropixels recordings, widefield calcium imaging, behavior, and session metadata accessed through the ONE API.
OpenNeuro
OpenNeuro is a free, open platform for sharing neuroimaging datasets, with public search, dataset pages, and download paths for web, S3, DataLad, and the OpenNeuro CLI.