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Fig 9 in Culicoides jiangchengensis, a new species of the subgenus Sinocoides (Diptera, Ceratopogonidae) based on integrative taxonomy from China

Fig 9. Culicoides (Sinocoides) malipoensis Liu et Ren, 2011 [13]. ant, antenna; f, front; map, maxillary palpus; md, mandibles; spt, spermatheca; w, wing. https://doi.org/10.1371/journal.pone.0287266.g009

Fig 8 in Culicoides jiangchengensis, a new species of the subgenus Sinocoides (Diptera, Ceratopogonidae) based on integrative taxonomy from China

Fig 8. Culicoides (Sinocoides) pungobovis Liu, Yan et Liu, 1996 [10]. ant, antenna; f, front; map, maxillary palpus; md, mandibles; spt, spermatheca; w, wing. https://doi.org/10.1371/journal.pone.0287266.g008

Fig 11 in Culicoides jiangchengensis, a new species of the subgenus Sinocoides (Diptera, Ceratopogonidae) based on integrative taxonomy from China

Fig 11. ML phylogenetic trees of COI gene nucleotide sequences of C. (Sinocoides) jiangchengensis from Jiangcheng County and Gongshan County, Yunnan Province, China using MAGE-X. The best DNA substitution model is GTR+G +I, and the trees were drawn using njplot 2.4 with the bootstrap value based on 1,000 replications. https://doi.org/10.1371/journal.pone.0287266.g011

Fig 6 in Culicoides jiangchengensis, a new species of the subgenus Sinocoides (Diptera, Ceratopogonidae) based on integrative taxonomy from China

Fig 6. multifarious Liu, Gong et Zhang, 2003 [11]. ant, antenna; f, front; map, maxillary palpus; md, mandibles; spt, spermatheca; w, wing. https://doi.org/10.1371/journal.pone.0287266.g006

Fig 4 in Culicoides jiangchengensis, a new species of the subgenus Sinocoides (Diptera, Ceratopogonidae) based on integrative taxonomy from China

Fig 4. Culicoides (Sinocoides) jinghongensis Wu et Liu, 2018 [14]. ant, antenna; f, front; map, maxillary palpus; md, mandibles; spt, spermatheca; w, wing. https://doi.org/10.1371/journal.pone.0287266.g004

Fig 5 in Culicoides jiangchengensis, a new species of the subgenus Sinocoides (Diptera, Ceratopogonidae) based on integrative taxonomy from China

Fig 5. Culicoides (Sinocoides) kongmiaoensis Liu et Zhou, 2006 [12]. Ant, antenna; f, front; map, maxillary palpus; md, mandibles; spt, spermatheca; w, wing. https://doi.org/10.1371/journal.pone.0287266.g005

Fig 4 in Using mating-type loci to improve taxonomy of the Tuber indicum complex, and discovery of a new species, T. longispinosum

Fig 4. Phylogenetic relationships among Asian black truffles based on MAT1-1-1 and MAT1-2-1 sequences. The phylogram was obtained by maximum likelihood inference under the TN93 model. SH-aLRT values and Bayesian posterior probabilities are shown as ML/BPP. https://doi.org/10.1371/journal.pone.0193745.g004

Fig 3 in Using mating-type loci to improve taxonomy of the Tuber indicum complex, and discovery of a new species, T. longispinosum

Fig 3. Relative frequencies of asci with one to six ascospores per ascus for the studied taxa. https://doi.org/10.1371/journal.pone.0193745.g003

Fig 2 in Using mating-type loci to improve taxonomy of the Tuber indicum complex, and discovery of a new species, T. longispinosum

Fig 2. Ascospore spine height of four-spored asci (A) and width of spine bases (B), for the studied taxa. Different letters above boxes indicate significant differences between mean according to Tukey–Kramer honestly significant difference test (P <0.01). https://doi.org/10.1371/journal.pone.0193745.g002

Fig 6 in Using mating-type loci to improve taxonomy of the Tuber indicum complex, and discovery of a new species, T. longispinosum

Fig 6. Tuber longispinosum photographs (holotype, TFM: S17009). A. Dried ascomata (bar = 1 cm). B. Fruit bodies photographed in the field. C. Peridial warts, (bar = 3 mm). D. Asci and ascospores (bar = 30 μm). E. Ascospore (bar = 10 μm). F. Peridium in cross section (bar = 50 μm). https://doi.org/10.1371/journal.pone.0193745.g006

Fig 1 in Using mating-type loci to improve taxonomy of the Tuber indicum complex, and discovery of a new species, T. longispinosum

Fig 1. SEM images of ascospores for Asian black truffles, showing the details of ornamentation. A–C: Tuber longispinosum (A: K70, B: K209, C: K466), D–F: Tuber sp. 6 (D: K152, E: S4, F: S23), G–I: T. formosanum (G: HKAS48268 paratype, H: HKAS62628 holotype, I: HKAS79547), J–L: T. himalayense (J: HP1-3, K: MY5-1, L: SHD1-1- 1), M–O: T. indicum (M: YSH1-8, N: YR1-4, O: HD6-16). Bars = 10 μm. https://doi.org/10.1371/journal.pone.0193745.g001

Fig 7 in Using mating-type loci to improve taxonomy of the Tuber indicum complex, and discovery of a new species, T. longispinosum

Fig 7. Tuber himalayense photographs (TFM: S17015). A. Ascomata (bar = 1 cm). B. Peridial warts, (bar = 3 mm). C. Asci and ascospores (bar = 30 μm). D. Peridium and glebal tissue in cross section (bar = 50 μm). E. Ascospore (bar = 10 μm). https://doi.org/10.1371/journal.pone.0193745.g007

FIGURE 12 in MORPHOLOGICAL VARIATION AND TAXONOMY OF LEPIDOCOLAPTES ANGUSTIROSTRIS (VIEILLOT, 1818) (PASSERIFORMES: DENDROCOLAPTIDAE)

FIGURE 12: Distribution of the two main morphotypes in Lepidocolaptes angustirostris. The "bivittatus" (no-streaked individuals) and the "angustirostris" groups (streaked individuals). Red-lined squares: no-streaked forms; Green squares: Streaked forms.

FIGURE 5 in MORPHOLOGICAL VARIATION AND TAXONOMY OF LEPIDOCOLAPTES ANGUSTIROSTRIS (VIEILLOT, 1818) (PASSERIFORMES: DENDROCOLAPTIDAE)

FIGURE 5: Distribution of ventral patterns found in Lepidocolaptes angustirostris. Orange square: Cinnamon-ochraceous; Yellow triangles: Pale yellow; White squares: Greyish-white; Blue diamonds: Greyish-white weakly streaked; Green circles: Dark brown streaked.

FIGURE 4B in MORPHOLOGICAL VARIATION AND TAXONOMY OF LEPIDOCOLAPTES ANGUSTIROSTRIS (VIEILLOT, 1818) (PASSERIFORMES: DENDROCOLAPTIDAE)

FIGURE 4B: Ventral streaked patterns identified in Lepidocolaptes angustirostris. Intermediate pattern (left, MZUSP 64173, Retiro da Telha, margem direita Rio Sucurui, Mato Grosso do Sul, Brazil). Rufous streaked pattern (middle, MZUSP 31795, Las cañitas, Tucumán, Argentina). Dark brown streaked pattern (right, MACN 30516-30517, Alto Rio Santa Maria, Orán, Salta, Argentina).

FIGURE 2 in MORPHOLOGICAL VARIATION AND TAXONOMY OF LEPIDOCOLAPTES ANGUSTIROSTRIS (VIEILLOT, 1818) (PASSERIFORMES: DENDROCOLAPTIDAE)

FIGURE 2: Dorsal patterns identified in Lepidocolaptes angustirostris. Strong brown pattern (left, MZUSP 63445, Fazenda Campos bons 38 km N. floresta, Pernambuco, Brazil). Strong brown-olive brown pattern (middle, MACN 217A, Gualeguaychu, Entre Rios, Argentina). Olive brown pattern (right, MACN 66339, Campus UNLP, Santa Rosa, La Pampa, Argentina).

FIGURE 3 in MORPHOLOGICAL VARIATION AND TAXONOMY OF LEPIDOCOLAPTES ANGUSTIROSTRIS (VIEILLOT, 1818) (PASSERIFORMES: DENDROCOLAPTIDAE)

FIGURE 3: Distribution of dorsal patterns in Lepidocolaptes angustirostris. Red square: Strong brown; Blue diamond: Intermediate; Orange star: Light Olive-brown.

FIGURE 1 in MORPHOLOGICAL VARIATION AND TAXONOMY OF LEPIDOCOLAPTES ANGUSTIROSTRIS (VIEILLOT, 1818) (PASSERIFORMES: DENDROCOLAPTIDAE)

FIGURE 1: Distribution specimens of Lepidocolaptes angustirostris analyzed in this study (red points) in South America.

FIGURE 4A in MORPHOLOGICAL VARIATION AND TAXONOMY OF LEPIDOCOLAPTES ANGUSTIROSTRIS (VIEILLOT, 1818) (PASSERIFORMES: DENDROCOLAPTIDAE)

FIGURE 4A: Ventral, unstreaked patterns identified in Lepidocolaptes angustirostris. Cinnamon-ochraceous pattern (left, MZUSP 63445, Fazenda Campos bons 38 km N. floresta, Pernambuco, Brazil). Pale yellow pattern (middle, MZUSP 77726, Parque Nacional da Serra das Confusões, Piauí, Brazil). Greyish-white pattern (right, MZUSP 29879, Rio Arica, Mato Grosso, Brazil).

FIGURE 6 in MORPHOLOGICAL VARIATION AND TAXONOMY OF LEPIDOCOLAPTES ANGUSTIROSTRIS (VIEILLOT, 1818) (PASSERIFORMES: DENDROCOLAPTIDAE)

FIGURE 6: Ventral patterns in the PCA analysis. Division between unstreaked and streaked populations, confidence level: 0.95.