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Figure 9 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure 9 Box plots of a length (bases) and b GC (%) content of ITS1 and ITS2 sequences for each one of the main lineages (Clades/Clusters) of the genus Ganoderma. The size of each box represents 50% of the values, the black horizontal line within each box indicates the median, the 'x' represents the average value, the error bars represent interquartile ranges and circles indicate outliers.

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Supplementary material 5 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure S2c

Figure 8 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure 8 Box plots of aITS sequence similarity (%) and b genetic distances (p-values) within (intra) and between (inter) Ganoderma species for each one of the main lineages (Clades/Clusters) of the genus, as well as pairwise comparisons between selected species. The size of each box represents 50% of the values, the black horizontal line within each box indicates the median, the 'x' represents the average value, the error bars represent interquartile ranges and circles indicate outliers. The red-dotted horizontal line, transversing the plots, represents the value levels accepted in this study for proposing new phylogenetic species.

Figure 6 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure 6 Detail from Fig. 3. Phylogenetic reconstruction of the genus Ganoderma inferred from ML analysis, based on ITS sequence data (main dataset, DS; Table 2) for Clades B, C and D. ML bootstrap values (BS) ≥ 65% and Bayesian Posterior Probabilities (BPP) ≥ 0.95 are shown. Sequences names on the left appear as initially labelled and are followed by the respective GenBank/ENA/DDBJ or UNITE accession number, while the total number of identical entries corresponding to a particular sequence is placed in parentheses, followed by the type of host plant (legend for the coloured shapes is found at the lower left side of the tree) and geographic origin of the respective material (the latter appears in different fonts colour depending on the continent of provenance; see also Table 1 and Suppl. material 1: Table S2). Species names on the right correspond to those inferred in this study evaluated in conjunction with literature data. Sequences generated in the present work appear in bold typeface, while underlined sequences are those originating from type material. Scale bar: 0.01 nucleotide substitutions per site.

Figure 5 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure 5 Detail from Fig. 3. Phylogenetic reconstruction of the genus Ganoderma inferred from ML analysis, based on ITS sequence data (main dataset, DS; Table 2) for Clade A, Cluster A.3. ML bootstrap values (BS) ≥ 65% and Bayesian Posterior Probabilities (BPP) ≥ 0.95 are shown. Sequences names on the left appear as initially labelled and are followed by the respective GenBank/ENA/DDBJ or UNITE accession number, while the total number of identical entries corresponding to a particular sequence is placed in parentheses, followed by the type of host plant (legend for the coloured shapes is found at the lower left side of the tree) and geographic origin of the respective material (the latter appears in different fonts colour depending on the continent of provenance; see also Table 1 and Suppl. material 1: Table S2). Species names on the right correspond to those inferred in this study evaluated in conjunction with literature data. Sequences generated in the present work appear in bold typeface, while underlined sequences are those originating from type material. Scale bar: 0.01 nucleotide substitutions per site.

Figure 7 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure 7 Detail from Fig. 3. Phylogenetic reconstruction of the genus Ganoderma inferred from ML analysis, based on ITS sequence data (main dataset, DS; Table 2) for Clade E. ML bootstrap values (BS) ≥ 65% and Bayesian Posterior Probabilities (BPP) ≥ 0.95 are shown. Sequences names on the left appear as initially labelled and are followed by the respective GenBank/ENA/DDBJ or UNITE accession number, while the total number of identical entries corresponding to a particular sequence is placed in parentheses, followed by the type of host plant (legend for the coloured shapes is found at the lower left side of tree) and geographic origin of the respective material (the latter appears in different fonts colour depending on the continent of provenance; see also Table 1 and Suppl. material 1: Table S2). Species names on the right correspond to those inferred in this study evaluated in conjunction with literature data. Sequences generated in the present work appear in bold typeface, while underlined sequences are those originating from type material. Scale bar: 0.01 nucleotide substitutions per site.

Figure 4 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure 4 Detail from Fig. 3. Phylogenetic reconstruction of the genus Ganoderma inferred from ML analysis, based on ITS sequence data (main dataset, DS; Table 2) for Clade A, Clusters A.1 and A.2. ML bootstrap values (BS) ≥ 65% and Bayesian Posterior Probabilities (BPP) ≥ 0.95 are shown. Sequences names on the left appear as initially labelled and are followed by the respective GenBank/ENA/DDBJ or UNITE accession number, while the total number of identical entries corresponding to a particular sequence is placed in parentheses, followed by the type of host plant (legend for the coloured shapes is found at the lower left side of tree) and geographic origin of the respective material (the latter appears in different font colour depending on the continent of provenance; see also Table 1 and Suppl. material 1: Table S2). Species names on the right correspond to those inferred in this study evaluated in conjunction with literature data. Sequences generated in the present work appear in bold typeface, while underlined sequences are those originating from type material. Scale bar: 0.01 nucleotide substitutions per site.

Figure 3 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure 3 Summary tree of the genus Ganoderma inferred from ML analysis, based on ITS sequence data (main dataset, DS; Table 2). Thick lines represent ML bootstrap values (BS) ≥ 65% and Bayesian Posterior Probabilities (BPP) ≥ 0.95. Clades and Clusters within the tree appear as presented in Table 1 and Suppl. material 1: Table S2. Species names correspond to those inferred in this study. Scale bar: 0.01 nucleotide substitutions per site.

Figure 2 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure 2 Basidiomes of Ganoderma spp. amongst those collected and analysed in this study (specimens codes appear in parantheses; Suppl. material 1: Table S1) aG. lucidum (A1180) bG. carnosum (DD1243) cG. resinaceum (2012-0077) dG. adspersum (2010-0015) eG. applanatum (DD2119) fG. pfeifferi (DD2118).

Figure 1 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure 1 a Initial labelling of 3908 Ganoderma sequences analysed in the present study: numbers in parentheses correspond to sequences deposited under the particular name in GenBank/ENA/DDBJ and UNITE, while species names appear underlined when ITS sequences derive from type material b final assigment of 3908 Ganoderma sequences to 80 species and six distinct groups as a result of the phylogenetic analyses performed in this study: numbers in parentheses correspond to the number of sequences grouped within each taxon (data deriving from Table 1 and Suppl. material 1: Tables S2, S4).

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Supplementary material 2 from: Fryssouli V, Zervakis GI, Polemis E, Typas MA (2020) A global meta-analysis of ITS rDNA sequences from material belonging to the genus Ganoderma (Basidiomycota, Polyporales) including new data from selected taxa. MycoKeys 75: 71-143. https://doi.org/10.3897/mycokeys.75.59872

Figure S1

Supplementary material 4 from: Mardones M, Carranza-Velázquez J, Mata-Hidalgo M, Amador-Fernández X, Urbina H (2023) Taxonomy and phylogeny of the genus Ganoderma (Polyporales, Basidiomycota) in Costa Rica. MycoKeys 100: 5-47. https://doi.org/10.3897/mycokeys.100.106810

Maximum Likelihood raw phylogenetic tree for ITS sequences of global Ganoderma

Supplementary material 1 from: Mardones M, Carranza-Velázquez J, Mata-Hidalgo M, Amador-Fernández X, Urbina H (2023) Taxonomy and phylogeny of the genus Ganoderma (Polyporales, Basidiomycota) in Costa Rica. MycoKeys 100: 5-47. https://doi.org/10.3897/mycokeys.100.106810

Ganoderma of Costa Rica_Linked Data Table Template for Primary Biodiversity Data

Supplementary material 2 from: Mardones M, Carranza-Velázquez J, Mata-Hidalgo M, Amador-Fernández X, Urbina H (2023) Taxonomy and phylogeny of the genus Ganoderma (Polyporales, Basidiomycota) in Costa Rica. MycoKeys 100: 5-47. https://doi.org/10.3897/mycokeys.100.106810

ITS alignment for global Ganoderma

Supplementary material 3 from: Mardones M, Carranza-Velázquez J, Mata-Hidalgo M, Amador-Fernández X, Urbina H (2023) Taxonomy and phylogeny of the genus Ganoderma (Polyporales, Basidiomycota) in Costa Rica. MycoKeys 100: 5-47. https://doi.org/10.3897/mycokeys.100.106810

Bayesian Inference raw phylogenetic tree for ITS sequences of global Ganoderma

Figure 8 from: Tang S-M, Chen D-C, Wang S, Wu X-Q, Ao C-C, Li E-X, Luo H-M, Li S-H (2024) Morphological and molecular analyses reveal two new species of Grifola (Polyporales) from Yunnan, China. MycoKeys 102: 267-284. https://doi.org/10.3897/mycokeys.102.118518

Figure 8 Grifola sinensis cultures characters (holotype HKAS 131995) A colony obverse on PDA B colony in reverse C terminal chlamydospore D clamped generative hyphae E, F chlamydospores. Photographs by Song-Ming Tang. Scale bars: 10 μm (C–F).

Figure 6 from: Tang S-M, Chen D-C, Wang S, Wu X-Q, Ao C-C, Li E-X, Luo H-M, Li S-H (2024) Morphological and molecular analyses reveal two new species of Grifola (Polyporales) from Yunnan, China. MycoKeys 102: 267-284. https://doi.org/10.3897/mycokeys.102.118518

Figure 6 Fresh basidiomata of Grifola sinensis (holotype HKAS 131995) A view of wild basidiomata pilei B view of wild basidiomata pores C, D cultivated basidiomata E view of pores by stereoscope F side view of pore zone and context by stereoscope. Photographs by Song-Ming Tang. Scale bars: 1 cm (A–D); 1 mm (E, F).

Figure 7 from: Tang S-M, Chen D-C, Wang S, Wu X-Q, Ao C-C, Li E-X, Luo H-M, Li S-H (2024) Morphological and molecular analyses reveal two new species of Grifola (Polyporales) from Yunnan, China. MycoKeys 102: 267-284. https://doi.org/10.3897/mycokeys.102.118518

Figure 7 Micromorphological features of Grifola sinensis (holotype HKAS 131995) A cuticle hyphae B pore edge C basidiospores D basidia. Photographs by Song-Ming Tang. Scale bars: 10 μm.

Figure 4 from: Tang S-M, Chen D-C, Wang S, Wu X-Q, Ao C-C, Li E-X, Luo H-M, Li S-H (2024) Morphological and molecular analyses reveal two new species of Grifola (Polyporales) from Yunnan, China. MycoKeys 102: 267-284. https://doi.org/10.3897/mycokeys.102.118518

Figure 4 Grifola edulis culture characters (holotype HKAS131996) A colony obverse on PDA B colony in reverse C terminal chlamydospore D clamped generative hyphae E–F chlamydospores. Photographs by Song-Ming Tang. Scale bars: 10 μm (C–F).

Figure 3 from: Tang S-M, Chen D-C, Wang S, Wu X-Q, Ao C-C, Li E-X, Luo H-M, Li S-H (2024) Morphological and molecular analyses reveal two new species of Grifola (Polyporales) from Yunnan, China. MycoKeys 102: 267-284. https://doi.org/10.3897/mycokeys.102.118518

Figure 3 Micromorphological features of Grifola edulis (holotype HKAS131996) A cuticle hyphae B pore edge C basidiospores D basidia. Photographs by Song-Ming Tang. Scale bars: 10 μm.