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Fig. 19 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 19. Punctation in front of the medial ocellus of females. A. Lasioglossum medinai (Vachal, 1895) (France: Uchaux). B. L. villosulum (Kirby, 1802) (Luxembourg: Stadtbredimus).

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Fig. 22 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 22. Geographical distribution of the two pseudocryptic species in the Western Palaearctic. A. Lasioglossum villosulum (Kirby, 1802). B. L. medinai (Vachal, 1895).

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Fig. 6. Melitta villosula Kirby, 1802 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 6. Melitta villosula Kirby, 1802, lectotype, ♂ (= Lasioglossum villosulum). A. Habitus. B. Head. C. Scutum. D. Propodeum. E. First tergum. F. Sterna.

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Fig. 8. Halictus pauperatulellus Strand, 1909 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 8. Halictus pauperatulellus Strand, 1909, holotype, ♂. A. Head. B. Scutum. C. Propodeum. D. First tergum. E. Metasoma, dorsal view. F Metasoma, ventral view.

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Fig. 21 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 21. Fringe on sternum 5 of males (arrow showing the fringe). A. Lasioglossum villosulum (Kirby, 1802). B. L. medinai (Vachal, 1895).

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Fig. 2 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 2. Colouration and relative size of the species and subspecies. A–B. Lasioglossum villosulum (Kirby, 1802), ♀ and ♂. C–D. L. medinai (Vachal, 1895), ♀ and ♂ (holotype). E–F. L. villosulum arabicum Ebmer, 2008, ♀ and ♂.

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Fig. 4 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 4. Ultrametric tree constructed using Bayesian inference and based on 29 haplotype sequences (658bp) of the cytochrome oxidase c subunit I gene of specimens currently identified as Lasioglossum villosulum (Kirby, 1802), Lasioglossum medinai (Vachal, 1895) and one as Lasioglossum berberum (Benoist, 1941). Each label corresponds to one roman letter which encompasses all sequence from a haplotype (for more details see Table 3). A. Lasioglossum medinai; B–D: three supported clusters (a fourth cluster could be defined in C) within Lasioglossum villosulum. This phylogenetic tree is rooted using Lasioglossum bluethgeni Ebmer, 1971 as outgroup (label III, voucher AP222). Posterior probabilities are given at nodes. The three colour gradients on the tree correspond to morphological delineation. Results of the species delimitations analyses are represented on the right side of the figure: the Bayesian Poisson Tree Process (bPTP) analyses based on the trees obtained using Bayesian inference (BI) or maximum likelihood (ML); The Generalized Mixed Yule Coalescent (GMYC) analysis resulting in seven candidate species (using the single threshold represented as a red line on the tree) and five alternative scenarios. Numbers at the top of the columns corresponds to the number of candidate species in the GMYC analysis.

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Fig. 1 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 1. Distribution of Lasioglossum villosulum (Kirby, 1802) throughout the Palaearctic and Oriental Regions.

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Fig. 15 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 15. Lasioglossum medinai (Vachal, 1895), holotype, ♂. A. Head. B. Scutum. C. Propodeum. D. Metasoma.

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Fig. 23 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 23. Lasioglossum berberum (Benoist, 1941), holotype, ♂. A. Head. B. Scutum. C. Propodeum. D. First tergum. E. Metasoma.

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Fig. 3 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 3. Trees constructed using maximum likelihood (above) and Bayesian inference (below) and based on 29 haplotype sequences (658bp) of the cytochrome oxidase c subunit I gene of specimens currently identified as Lasioglossum villosulum (Kirby, 1802), Lasioglossum medinai (Vachal, 1895) and one as Lasioglossum berberum (Benoist, 1941). Each label corresponds to one roman letter which encompasses all sequences from a haplotype (for more details see Table 3). A, Lasioglossum medinai; B–D: Lasioglossum villosulum. Label highlighted in blue corresponds to L. berberum. This phylogenetic tree is rooted using Lasioglossum bluethgeni Ebmer, 1971 as outgroup (label III, voucher AP222). Bootstrap support (%) and posterior probabilities are given at nodes.

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Fig. 10. Halictus villiersi Benoist, 1941 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 10. Halictus villiersi Benoist, 1941, holotype, ♀. A. Head. B. Scutum. C. Propodeum. D. First tergum. E. Metasoma.

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Fig. 18 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 18. Punctation of the first tergum of females. A. Lasioglossum medinai (Vachal, 1895) (France: Uchaux). B. L. villosulum (Kirby, 1802) (France: Visan).

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Fig. 14. Lasioglossum villosulum arabicum Ebmer, 2008 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 14. Lasioglossum villosulum arabicum Ebmer, 2008, ♂ (UAE). A. Head. B. Scutum. C. Propodeum. D. First tergum. E. Metasoma.

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Fig. 9. Halictus rufotegularis Cockerell, 1938 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 9. Halictus rufotegularis Cockerell, 1938, holotype, ♀. A. Habitus, dorsal view. B. Head. C. Scutum. D. Propodeum. E. First tergum. F. Metasoma.

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Fig. 12. Heads. A in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 12. Heads. A. Lasioglossum villosulum villosulum (Kirby, 1802), ♀ (France, Allier). B. L. medinai, ♀ (France, Vaucluse). C. L. villosulum trichopse (Strand, 1914), ♀ (Taiwan). D. Idem, ♂.

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Fig. 17 in Integrative taxonomy resuscitates two species in the Lasioglossum villosulum complex (Kirby, 1802) (Hymenoptera: Apoidea: Halictidae)

Fig. 17. Propodeum sculpture of females. A. Lasioglossum medinai (Vachal, 1895) (France: Uchaux). B. L. villosulum (Kirby, 1802).

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Fig. 16 in Integrative taxonomy of five astome ciliates (Ciliophora, Astomatia) isolated from earthworms in Central Europe

Fig. 16. Alignment of variable positions of the 18S rRNA gene of five astome ciliates isolated from the lumbricid earthworms. Boxes mark 25 nucleotide positions in which M. lumbrici (Dujardin, 1841) differs from M. varians (de Puytorac, 1954). The comparison with three outgroup species of Anoplophrya Stein, 1860 indicates that 19 out of the 25 variable nucleotide positions of M. lumrici are either plesiomorphies or possibly homoplasies.

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Fig. 7 in Integrative taxonomy of five astome ciliates (Ciliophora, Astomatia) isolated from earthworms in Central Europe

Fig. 7. Metaradiophrya varians (de Puytorac, 1954), Slovak specimens in vivo. A, D. Ventral view of representative specimens, showing the typical body shape, localization of the fibrillar hook, the long rodlike macronucleus and two staggered rows of contractile vacuoles (arrowheads). B. Detail of the anterior body portion, showing the fibrillar hook and its associated fibers. There are 5 or 6 fibers attached to the upper right side of the longer arm, on average 26 (22–29) fibers to the ventral side of the longer arm and 11 or 12 fibers to the left side of the shorter arm. Arrowheads mark the subapical suture extending from the right body margin over the fibrillar hook towards the left body margin. C. Dorsal view, showing the somatic kineties. The ciliary rows are narrowly arranged and are composed of very densely spaced basal bodies (left inset). E. Ventral view, showing a late divider. Scale bars: A, C–E = 50 µm; B = 20 µm.

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Fig. 2 in Integrative taxonomy of five astome ciliates (Ciliophora, Astomatia) isolated from earthworms in Central Europe

Fig. 2. Metaradiophrya lumbrici (Dujardin, 1841), Slovak specimens in vivo. A. Semi-schematic diagram of the ventral side, showing the fibrillar hook as well as the contractile vacuole and the somatic ciliary pattern. Arrowheads mark the subapical suture extending from the right body margin over the hook towards the left body margin. B. Detail of the anterior body portion, showing the fibrillar hook and its associated fibers. There are on average 6 (5–7) fibers attached to the upper right side of the longer arm, on average 33 (30–37) fibers to the ventral side of the longer arm and on average 11 (8–13) fibers to the left side of the shorter arm. C. Shape variants of fibrillar hooks. The hook is composed of two unequally long arms: the longer arm is flat and 25–35 µm long, while the shorter arm appears slightly more robust at the base and is 8–13 µm long. D–E. Lateral somatic kineties form a right and a left subterminal suture in the posterior body region. F. Ventral view, showing the general body organization. G. The cytoplasm contains innumerable granules being ca 0.4 µm across and rod-like bacteria being about 3–15 µm long. Scale bars: A, F = 50 µm; B = 10 µm.

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