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FIGURE 1. Maximum parsimony 16S in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 1. Maximum parsimony 16S rRNA phylogram for species in the Boophis majori and B. microtympanum groups. From 480 total characters, 336 were constant and 129 parsimony informative. MP searches retained 39 trees of which a strict consensus is shown. Consensus support values higher than 50, from 2000 bootstrap replicates, are shown; an asterisk indicates Bayesian posterior probabilities equal or higher than 95%. Species newly described or resurrected herein are in bold.

FIGURE 18. Maximum parsimony 16S in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 18. Maximum parsimony 16S rRNA phylogram for species in the Boophis luteus group. From 470 total characters, 347 were constant and 111 parsimony informative. MP searches retained 60 trees of which a strict consensus is shown. Consensus support values higher than 50, from 2000 bootstrap replicates, are shown; an asterisk indicates Bayesian posterior probabilities equal or higher than 95%. The species newly described herein is in bold.

FIGURE 12 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 12. Male holotype of Boophis roseipalmatus sp. nov. (ZSM 211/2004) from Montagne d'Ambre National Park: (A) dorsolateral view; (B) ventral view.

FIGURE 3 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 3. Spectrograms and waveforms of calls of Boophis piperatus sp. nov. from near Vohiparara, Ranomafana National Park (recorded on 28 January 2004, air temperature 20–21°C): (A) call type 1, (B) call type 2 (see text).

FIGURE 16. Maximum parsimony 16S in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 16. Maximum parsimony 16S rRNA phylogram for species in the Boophis albilabris group. From 535 total characters, 445 were constant and 35 parsimony informative. MP searches retained 6 trees of which a strict consensus is shown. Consensus support values higher than 50, from 2000 bootstrap replicates, are shown; an asterisk indicates Bayesian posterior probabilities equal or higher than 95%. The species newly described herein is in bold.

FIGURE 4 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 4. Dorsolateral view (A) and ventral view (B) of male of Boophis andrangoloaka (FGZC 2139) from Ambohitantely; (C) dorsolateral view of Boophis andrangoloaka (ZFMK 60134) from Ambohitantely; dorsolateral view (D) and ventral view (E) of a dark coloured specimen of Boophis rhodoscelis from Ranomafanakely; (F) dorsolateral view of a light coloured specimen of Boophis rhodoscelis from Ranomafanakely.

FIGURE 24 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 24. Spectrogram and waveform of advertisement calls of: (A) Boophis miadana sp. nov. from Andohahela National Park, 1550 m a.s.l. (recorded on 26 January 2005, air temperature app. 17.6°C); (B) Boophis haingana sp. nov. from Andohahela National Park, low altitude (recorded on 3 February 2004, air temperature app. 23.2°C).

FIGURE 15 in Integrative taxonomy of Malagasy treefrogs: combination of molecular genetics, bioacoustics and comparative morphology reveals twelve additional species of Boophis 2383

FIGURE 15. Spectrograms and waveforms of calls: (A) call type 1 of Boophis obscurus from near Vohiparara, Ranomafana National Park (recorded on 29 January 2004, air temperature app. 20.5°C); (B) advertisement call of Boophis periegetes from Andohahela National Park (recorded on 27 January 2005, air temperature app. 18°C)

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Supplementary Table S1 and Supplementary Table S2 - Part of "Next generation leishmanization: revisiting molecular targets for selecting genetically engineered live-attenuated Leishmania"

<p>Supplementary Table S1 and Supplementary Table S2 -</p> <p>Part of "Next generation leishmanization: revisiting molecular targets for selecting genetically engineered live-attenuated Leishmania"</p>

FIGURE 3. A in A new species of barred frog, Mixophyes (Anura: Myobatrachidae) from south-eastern Australia identified by molecular genetic analyses

FIGURE 3. A) Maximum Likelihood phylogram and B) SVD Quartets cladogram of relationships among Mixophyes based on Single Nucleotide Polymorphisms. Alphanumeric codes refer to collection location details and ABTC numbers in Table 1.

FIGURE 6 in A new species of barred frog, Mixophyes (Anura: Myobatrachidae) from south-eastern Australia identified by molecular genetic analyses

FIGURE 6. Cross bar patterns on hindlimbs of Mixophyes. Side by side comparison of A) thigh and B) tibia-fibula in life of left Mixophyes balbus from Coombadjha Creek Washpool NP and right M. australis sp. nov. from Congewai Creek, Watagan NP. Thighs of Mixophyes balbus from C) Little Styx River, New England NP, D) Thungutti Creek, New England NP, E) Coombadjha Creek, Washpool NP, and Mixophyes australis sp. nov. from F) Congewai Creek, Watagan NP, G) Frying Pan Creek, Chichester SF and H) Sharpes Creek, Gloucester Tops NP.

FIGURE 5 in A new species of barred frog, Mixophyes (Anura: Myobatrachidae) from south-eastern Australia identified by molecular genetic analyses

FIGURE 5. Plots of linear discriminants for analyses of morphological variation among Mixophyes balbus (blue) and Mixophyes australis sp. nov. (red).

FIGURE 7 in A new species of barred frog, Mixophyes (Anura: Myobatrachidae) from south-eastern Australia identified by molecular genetic analyses

FIGURE 7. Mixophyes balbus in life. A) Rockadooie Ck, Curramore SF, Michael Mahony, B) Coombadjha Ck, Washpool NP, Stephen Mahony, C) Washpool NP, Gibraltar Range, Stephen Mahony, D) Coombadjha Ck, Washpool NP, Stephen Mahony, E) Forestland SF, Stephen Mahony, F) Forestland SF, Stephen Mahony.

FIGURE 10 in A new species of barred frog, Mixophyes (Anura: Myobatrachidae) from south-eastern Australia identified by molecular genetic analyses

FIGURE 10. Comparison of geographic pattern of relationships among mitochondrial DNA sequences and nuclear genetic (SNP) groups, highlighting the discordance in relationships among samples inferred from the two genomic compartments. Refer to Table 1 for locality code information and ABTC numbers.

FIGURE 4 in A new species of barred frog, Mixophyes (Anura: Myobatrachidae) from south-eastern Australia identified by molecular genetic analyses

FIGURE 4. Single Nucleotide Polymorphism Principal Component Analysis of Mixophyes fleayi and M. balbus. A) PC1 v PC2, B) PC3 v PC4, C) STRUCTURE barplot of all ingroup samples, D) STRUCTURE barplot of southern group samples only.

FIGURE 2 in A new species of barred frog, Mixophyes (Anura: Myobatrachidae) from south-eastern Australia identified by molecular genetic analyses

FIGURE 2. Bayesian phylogram of relationships among mitochondrial ND2 haplotypes in Mixophyes. Values at nodes represent ML bootstrap proportions (left) and Bayesian posterior probabilities (right). The tree was rooted with sequences from the outgroups and Lechriodus melanopyga, Limnodynastes salmini and Neobatrachus pictus (Table 1). Alphanumeric codes refer to collection location details and ABTC numbers in Table 1.

FIGURE 8 in A new species of barred frog, Mixophyes (Anura: Myobatrachidae) from south-eastern Australia identified by molecular genetic analyses

FIGURE 8. Mixophyes australis sp. nov. in life. A) Holotype AMS R188750, Sharpes Creek, Gloucester Top NP, Ross Knowles, B) Sharpes Creek, Gloucester Top NP, C) Gap Creek, Watagan NP, Stephen Mahony, D) Abbots Ck, Olney SF, Stephen Mahony, E) Cockerawombeeba Ck, Hastings Range, Michael Mahony, F) Cockerawombeeba Ck, Hastings Range, Michael Mahony.

FIGURE 1 in A new species of barred frog, Mixophyes (Anura: Myobatrachidae) from south-eastern Australia identified by molecular genetic analyses

FIGURE 1. Map showing collection locations sampled for molecular genetic analysis of Mixophyes in eastern Australia. Alphanumeric codes refer to collection location details in Table 1. Small black dots are voucher records from the Atlas of Living Australia, yellow triangles—M. fleayi, blue circles—M. balbus northern group, red squares—M. balbus southern group.

FIGURE 9 in A new species of barred frog, Mixophyes (Anura: Myobatrachidae) from south-eastern Australia identified by molecular genetic analyses

FIGURE 9. Reproductive calls of Mixophyes balbus and Mixophyes australis sp. nov. A) Oscillogram and spectrogram of a series of advertisement calls and encounter calls of Mixophyes australis sp. nov. over 40 seconds duration, B) a single call with the time axis expanded (1.5 seconds duration), C) oscillogram and spectrogram of a series of advertisement calls and encounter calls of Mixophyes balbus over 40 seconds duration, D) a single call with the time axis expanded (1.5 seconds duration), E) oscillogram and spectrogram of an encounter call of Mixophyes australis sp. nov., (0.4 second time axis), and F) oscillogram and spectrogram of an encounter call Mixophyes balbus (0.4 second time axis). a = advertisement call, e = encounter call, and kHz is kilohertz.