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opencc-by-4.0Sep 2022View details →

Three-dimensional surface scans of tooth surfaces of Sika deer from Torihama shell midden site

Three-dimensional surface scans of tooth surfaces of Sika deer remains from Torihama shell midden site are saved in .sur format and surface analyses files in .mnt format, which are analyzed in: Sato, K., and Sato, T., and Kubo, M. O. "Reconstructing diets of hunted sika deer from Torihama shell midden site (ca. 6000 years ago) by dental microwear texture analysis" submitted to Frontiers in Ecology and Evolution. doi: 10.3389/fevo.2022.957038 More details can be found in the information published in this paper.

opencc-by-4.0Sep 2022View details →

Python scripts / Jupyter Notebooks and data for training segmentation models on slide scans of diatom preparations from river Menne

This archive contains the Jupyter Notebooks and data used for the deep learning experiments published in Kloster et al. 2022: Improving deep learning-based segmentation of diatoms in gigapixel-sized virtual slides by object-based tile positioning and object integrity constraint. The notebooks are numbered according to the order in which they are to execute. Please refer to the comments and documentation within the notebooks as well as to the manuscript for details. The data (image data, mask data & segmentation ground truth in COCO format for several different tiling strategies) is stored in separate subfolders corresponding with data usage (model training, validation, test) and tiling strategy. Please refer to the "readme" files for detailed information.

opencc-by-4.0Sep 2022View details →

Segmentation models and experimental results on segmenting slide scans of diatom preparations from river Menne

This archive contains the models and the results of the deep learning experiments published in Kloster et al. 2022: Improving deep learning-based segmentation of diatoms in gigapixel-sized virtual slides by object-based tile positioning and object integrity constraint. The folders contain models and results of each of the 24 training runs, the files “metrics.experiments.pt[prediction score threshold].csv contain segmentation score thresholds the models obtained on unknown evaluation data. The data pertaining to each model is stored in a separate folder. Its name follows the convention “experiment.[model architecture].[tiling method].[dataset size].[timestamp]”. Please note that the naming of the tiling method differs from the manuscript; “fixed” refers to fixed-stride tiling, “objectcentred” object-based positioning, and “objectcentred_with_cropped” to object-based positioning + object integrity constraint. Dataset size “10p” refers to a 10% subsample of the complete training data set, “25p” to a 25% subsample and so on. Each folder contains the best performing model of the corresponding training run as pth (Mask R-CNN, PyTorch) or h5 (U-Net, Tensorflow/Keras) file, along with a files describing setup and conduction of the training. Subfolders “test_images_segmented*” contain the segmentation predicted by the models on the evaluation data. These are supplied as 1.) mask image either with the intensity value representing the prediction score (score 0.0 – 1.0 = intensities 0 – 255) or thresholded by the prediction score threshold given in the folder name; 2.) input image overlayed with ground truth (red) and segmentation mask (green), resulting in TP marked in yellow; 3.) a CSV file giving info on the filenames and the segmentation performance metrics. Please refer to the manuscript for further details.

dryad40/100

Detection of standing retention trees in boreal forests with airborne laser scanning point clouds and multispectral imagery

1. In a landscape consisting primarily of intensive forestry interspersed with some protected areas, multifunctional forestry with retention trees can play a crucial role in nature conservation. Accurate mapping of retention trees is important for guiding landscape-level conservation and forest management and improving landscape connectivity. Sizeable dead and living retention trees play a particularly important ecological role but even their large-scale inventory is often intensive through field work and/or inaccurate. We aimed to detect and classify retention trees using the novel nationwide Finnish airborne laser scanning (ALS) data (~ 5 pulses/m2) in conjunction with unrectified color-infrared (CIR) aerial imagery. 2. Applying photogrammetric principles, we added spectral information from the CIR imagery to the ALS-derived point cloud. For a training dataset of 160 retention trees from 19 stands and a geographically separate validation dataset of 79 trees from 8 stands, we segmented trees via individual tree detection (ITD), removed most trees belonging to the regenerating vegetation layer, and classified trees into living conifers, living broadleaves, and dead trees by linear discriminant analysis. 3. The detection rate via ITD differed considerably for dead and living trees, with 41.7% of all dead and 83.8% of all living trees being detected with relatively low commission error rates. Dead trees with smaller diameters and heights were more likely missed, while grouping caused living tree omission. For classification into living conifers, living broadleaves, and dead trees, an overall accuracy of 67.3% was achieved in training and 71.2% in validation data only ALS-derived metrics. When adding spectral metrics, the overall accuracies were 79.6% and 61.0% for training and validation, respectively. 4. Our findings imply that wall-to-wall large-scale high density ALS data can be used to detect retention trees rather accurately – even larger dead trees – and that metrics derived solely from ALS data can accurately classify detected retention trees into living conifers, living broadleaves, and dead trees. Considering the ecological value of retention trees, our results are promising and indicate that ALS data of the studied pulse density are a cost-effective option for large area mapping of retention trees in countries with such data available.

opencc-zeroSep 2022View details →

Text-fig. 8. Scanning electron micrographs (a–f, h) and X-ray microtomographic orthoslices (g) of flowers from Zliv-Řídká Blana locality. a: Taxon 27, epigynous flower, no. NM-F 4504; b: Taxon 30, epigynous flower, remains of two thick sepals, a massive nectary disk (arrowhead) and two styles, no. NM-F 3199; c: Taxon 29, flower with stamens have long filament, calyx (ca) and corolla (co), no. NM-F 3198; d: Taxon 32, flower, no. NM-F 4503; e–h: Taxon 14, e – hypogenous flower, no. NM-F 3196, f – floral bud with a thick pedicel, no. NM-F 3196, g – flower with gynoecium showing central placentation and several seeds, no. NMF 3196, h – flower with gynoecium showing several seeds, no. NM-F 4505. in Plant Mesofossils From The Late Cretaceous Klikov Formation, The Czech Republic

Text-fig. 8. Scanning electron micrographs (a–f, h) and X-ray microtomographic orthoslices (g) of flowers from Zliv-Řídká Blana locality. a: Taxon 27, epigynous flower, no. NM-F 4504; b: Taxon 30, epigynous flower, remains of two thick sepals, a massive nectary disk (arrowhead) and two styles, no. NM-F 3199; c: Taxon 29, flower with stamens have long filament, calyx (ca) and corolla (co), no. NM-F 3198; d: Taxon 32, flower, no. NM-F 4503; e–h: Taxon 14, e – hypogenous flower, no. NM-F 3196, f – floral bud with a thick pedicel, no. NM-F 3196, g – flower with gynoecium showing central placentation and several seeds, no. NMF 3196, h – flower with gynoecium showing several seeds, no. NM-F 4505.

Text-fig. 7. Scanning electron micrographs (a, b, d, e, g–k), X-ray microtomographic orthoslices (c) and synchrotron radiation X-ray tomographic microscopy orthoslices (f) of fruits and endocarps of uncertain affinity from Zliv-Řídká Blana locality. a–c: Trebecenia sarcocalis, a – tricarpellate fruit, no. NM-F 3637, b – fruits supported by pentamerous and persistent calyx, no. NMF 3637, c – fruit almost circular in transverse section, no. NM-F 3637; d: Taxon 17, small fruit with slightly sunken stylar region, no. NM-F 3201; e: Taxon 19, spherical fruit, the fruit wall composed of large isodiametric, thick walled cells, no. NM-F 3181; f: Taxon 19, single-seeded fruit, no. NM-F 3621; g: Taxon 20, syncarpous, multicarpellate fruit of ten carpels, no. NM-F 3200; h: Taxon 22, syncarpous, multicarpellate fruit of seven carpels, no. NM-F 3159; i: cf. Sabia menispermoides, endocarp of drupaceous fruits, no. NM-F 4624; j: Taxon 25, endocarp triangular in cross-section, no. NM-F 3218; k: Taxon 24, endocarp spherical in cross-section with a distinctly ribbed and foveolate surface, no. NM-F 3217. in Plant Mesofossils From The Late Cretaceous Klikov Formation, The Czech Republic

Text-fig. 7. Scanning electron micrographs (a, b, d, e, g–k), X-ray microtomographic orthoslices (c) and synchrotron radiation X-ray tomographic microscopy orthoslices (f) of fruits and endocarps of uncertain affinity from Zliv-Řídká Blana locality. a–c: Trebecenia sarcocalis, a – tricarpellate fruit, no. NM-F 3637, b – fruits supported by pentamerous and persistent calyx, no. NMF 3637, c – fruit almost circular in transverse section, no. NM-F 3637; d: Taxon 17, small fruit with slightly sunken stylar region, no. NM-F 3201; e: Taxon 19, spherical fruit, the fruit wall composed of large isodiametric, thick walled cells, no. NM-F 3181; f: Taxon 19, single-seeded fruit, no. NM-F 3621; g: Taxon 20, syncarpous, multicarpellate fruit of ten carpels, no. NM-F 3200; h: Taxon 22, syncarpous, multicarpellate fruit of seven carpels, no. NM-F 3159; i: cf. Sabia menispermoides, endocarp of drupaceous fruits, no. NM-F 4624; j: Taxon 25, endocarp triangular in cross-section, no. NM-F 3218; k: Taxon 24, endocarp spherical in cross-section with a distinctly ribbed and foveolate surface, no. NM-F 3217.

Text-fig. 6. Scanning electron micrographs of seeds of ericalean affinity (a–d) and seeds of uncertain affinity (e–i) from Zliv-Řídká Blana locality. a: Protovisnea sp. 1, rounded seed with the narrow elongate seed cavity flanked by two bulging regions of larger cells, no. NM-F 3177; b: Protovisnea sp. 2, angular seed with the narrow elongate seed cavity flanked by two bulging regions of larger cells, no. NM-F 3179; c, d: Eurya crassitesta, one seed split into two parts, no. NM-F 3211, c – surface cells of the seed coat are palisade, d – cross-section of the seed; e: Nympheaceae sp. 1, seed, no. NM-F 3636; f: Nympheaceae sp. 2, seed, no. NM-F 4634; g: Klikovispermum sp.1, seeds with irregular outline and smooth outer surface, no. NM-F 3203; h: Klikovispermum malechii, seed with an orange-segment shape, no. NM-F 3299; i: Taxon 35, seed, no. NM-F 3236. in Plant Mesofossils From The Late Cretaceous Klikov Formation, The Czech Republic

Text-fig. 6. Scanning electron micrographs of seeds of ericalean affinity (a–d) and seeds of uncertain affinity (e–i) from Zliv-Řídká Blana locality. a: Protovisnea sp. 1, rounded seed with the narrow elongate seed cavity flanked by two bulging regions of larger cells, no. NM-F 3177; b: Protovisnea sp. 2, angular seed with the narrow elongate seed cavity flanked by two bulging regions of larger cells, no. NM-F 3179; c, d: Eurya crassitesta, one seed split into two parts, no. NM-F 3211, c – surface cells of the seed coat are palisade, d – cross-section of the seed; e: Nympheaceae sp. 1, seed, no. NM-F 3636; f: Nympheaceae sp. 2, seed, no. NM-F 4634; g: Klikovispermum sp.1, seeds with irregular outline and smooth outer surface, no. NM-F 3203; h: Klikovispermum malechii, seed with an orange-segment shape, no. NM-F 3299; i: Taxon 35, seed, no. NM-F 3236.

Text-fig. 5. Scanning electron micrographs (a, b, d–f) and X-ray microtomographic orthoslices (c) of capsular fruits composed of three carpels fruits and fragment of a capsular fruit from Zliv-Řídká Blana locality. a–c: Taxon 12, a – capsules of broadly elliptical shape, no. NM-F3302, b – tricarpellate capsules in apical view, no. NM-F 3302, c – tricarpellate capsules with pentamerous calyx, no. NM-F3302; d, e: Taxon 13, d – tricarpellate capsules of broadly elliptical shape, no. NM-F 4501, e – tricarpellate capsules in apical view, no. NM-F 4501; f: Taxon 11, fragment of a capsular fruit, no. NM-F 4622. in Plant Mesofossils From The Late Cretaceous Klikov Formation, The Czech Republic

Text-fig. 5. Scanning electron micrographs (a, b, d–f) and X-ray microtomographic orthoslices (c) of capsular fruits composed of three carpels fruits and fragment of a capsular fruit from Zliv-Řídká Blana locality. a–c: Taxon 12, a – capsules of broadly elliptical shape, no. NM-F3302, b – tricarpellate capsules in apical view, no. NM-F 3302, c – tricarpellate capsules with pentamerous calyx, no. NM-F3302; d, e: Taxon 13, d – tricarpellate capsules of broadly elliptical shape, no. NM-F 4501, e – tricarpellate capsules in apical view, no. NM-F 4501; f: Taxon 11, fragment of a capsular fruit, no. NM-F 4622.

Text-fig. 3. Scanning electron micrographs of Normapolles flowers/fruits from Zliv-Řídká Blana locality. a: Budvaricarpus serialis, aggregation of laterally fused fruits supported by a common bract (br – bract, hp – hypanthium), no. NM-F 3160; b: Budvaricarpus sp., aggregation of laterally fused fruits supported by several bracts, no. NM-F 3619; c: Caryanthus trebecnsis, reproductive unit consists of three bisexual, epigynous flowers/young fruits, no. NM-F 3286; d: Dahlergeniantus sp., hypogynous flower with radial symmetry, no. NM-F 4495; e: Calathiocarpus sp., epigynous flower with radial symmetry, no. NM-F 4631; f: Caryanthus sp. 1, ribbed fruit–nut of deltoid shape, no. NM-F 3208; g: Zlivifructus vachae, bisymmetrical flower with four tepals and four stamens, no. NM-F 3172; h: Taxon 3, ribbed fruit of Normapolles affinity, no. NM-F 3724. in Plant Mesofossils From The Late Cretaceous Klikov Formation, The Czech Republic

Text-fig. 3. Scanning electron micrographs of Normapolles flowers/fruits from Zliv-Řídká Blana locality. a: Budvaricarpus serialis, aggregation of laterally fused fruits supported by a common bract (br – bract, hp – hypanthium), no. NM-F 3160; b: Budvaricarpus sp., aggregation of laterally fused fruits supported by several bracts, no. NM-F 3619; c: Caryanthus trebecnsis, reproductive unit consists of three bisexual, epigynous flowers/young fruits, no. NM-F 3286; d: Dahlergeniantus sp., hypogynous flower with radial symmetry, no. NM-F 4495; e: Calathiocarpus sp., epigynous flower with radial symmetry, no. NM-F 4631; f: Caryanthus sp. 1, ribbed fruit–nut of deltoid shape, no. NM-F 3208; g: Zlivifructus vachae, bisymmetrical flower with four tepals and four stamens, no. NM-F 3172; h: Taxon 3, ribbed fruit of Normapolles affinity, no. NM-F 3724.

Text-fig. 2. Scanning electron micrographs of non-angiosperm remains (a–d) and insect remains (e–f) from Zliv-Řídká Blana locality. a: Eopolytrichium sp. small leafy shoot, no. NM-F 3631; b: Taxon 1, single tip of a young fern frond with circinate vernation, no. NM-F 4130; c: Taxon 2, fern with simple leaves and circinate vernation, no. NM-F 3459; d: Pagiophyllum sp., small needle-like leaf, no. NM-F 4132; e: Microcarpolithes hexagonalis, a faecal pellet/coprolite with subcylindrical shape, no. NMF 4520; f: Palaeoaldrovanda splendens, pieces of compact walls formed by rectangular cells, no. NM-F 3237. in Plant Mesofossils From The Late Cretaceous Klikov Formation, The Czech Republic

Text-fig. 2. Scanning electron micrographs of non-angiosperm remains (a–d) and insect remains (e–f) from Zliv-Řídká Blana locality. a: Eopolytrichium sp. small leafy shoot, no. NM-F 3631; b: Taxon 1, single tip of a young fern frond with circinate vernation, no. NM-F 4130; c: Taxon 2, fern with simple leaves and circinate vernation, no. NM-F 3459; d: Pagiophyllum sp., small needle-like leaf, no. NM-F 4132; e: Microcarpolithes hexagonalis, a faecal pellet/coprolite with subcylindrical shape, no. NMF 4520; f: Palaeoaldrovanda splendens, pieces of compact walls formed by rectangular cells, no. NM-F 3237.

Text-fig. 4. Scanning electron micrographs (a, c, e–k) and X-ray microtomographic orthoslices (b, d) of capsular fruits compose of five carpels from Zliv-Řídká Blana locality. a–d: Taxon 4, a – fruit elliptical in shape, no. NM-F 3188, b – young fruit with reminisce of free styles at top and showing central placentation of seeds, no. NM-F 3188, c – pentacarpellate capsules in apical view, no. NM-F 3188, d – fruit with five locules, no. NM-F 3235; e, f: Taxon 6, e – elongated fruit in lateral view, the persistent perianth at the base of the fruit (arrowhead), no. NM-F 3194, f – fruit in apical view, no. NM-F 3194; g, h: Taxon 5, g – elongated fruit in lateral view, no. NM-F 3193, h – fruit showing remains of a persistent calyx in the basal part (arrowhead), no. NM-F 3193; i–k: Taxon 7, i – pentacarpellate capsules of broadly elliptical shape, no. NM-F 4091, j – fruit, apical view, no. NM-F 4091, k – fruit with five seeds (arrowheads) ellipsoidal or triangular in outline and with a thick seed coat, no. NM-F 4091. in Plant Mesofossils From The Late Cretaceous Klikov Formation, The Czech Republic

Text-fig. 4. Scanning electron micrographs (a, c, e–k) and X-ray microtomographic orthoslices (b, d) of capsular fruits compose of five carpels from Zliv-Řídká Blana locality. a–d: Taxon 4, a – fruit elliptical in shape, no. NM-F 3188, b – young fruit with reminisce of free styles at top and showing central placentation of seeds, no. NM-F 3188, c – pentacarpellate capsules in apical view, no. NM-F 3188, d – fruit with five locules, no. NM-F 3235; e, f: Taxon 6, e – elongated fruit in lateral view, the persistent perianth at the base of the fruit (arrowhead), no. NM-F 3194, f – fruit in apical view, no. NM-F 3194; g, h: Taxon 5, g – elongated fruit in lateral view, no. NM-F 3193, h – fruit showing remains of a persistent calyx in the basal part (arrowhead), no. NM-F 3193; i–k: Taxon 7, i – pentacarpellate capsules of broadly elliptical shape, no. NM-F 4091, j – fruit, apical view, no. NM-F 4091, k – fruit with five seeds (arrowheads) ellipsoidal or triangular in outline and with a thick seed coat, no. NM-F 4091.

Text-fig. 3. CT slices on Block 3. Invertebrate moulds (a, c) and remains of their hard skeletons (a, b). Large areas of limestone matrix hold either only a few scattered invertebrates or no fossil at all (b, c). Ring artefacts seen close to the isocentre of the scan (b, c) are a well-known phenomenon caused by the X-ray beams traversing the block at an insufficient radiation dose (as expected in such a large block of dense material), and are not part of any physical structure present therein (Triche et al. 2019). in Hidden Treasures Uncovered: Successful Detection Of Fossils Below The Surface In Large Limestone Blocks Using A Standard Medical X-Ray Ct Scanner

Text-fig. 3. CT slices on Block 3. Invertebrate moulds (a, c) and remains of their hard skeletons (a, b). Large areas of limestone matrix hold either only a few scattered invertebrates or no fossil at all (b, c). Ring artefacts seen close to the isocentre of the scan (b, c) are a well-known phenomenon caused by the X-ray beams traversing the block at an insufficient radiation dose (as expected in such a large block of dense material), and are not part of any physical structure present therein (Triche et al. 2019).

Text-fig. 5. Mastixiopsis nyssoides KIRCHH. a, b, g–n: Organic preservation. a, b: Lignitic, unpermineralized, early Eocene Dorset Pipe clays at Arne, V. 40762. a: Ventral view (original illustration from pl. 18, fig. 1 of Chandler 1962). b: Transverse fracture, somewhat distorted by compression. c–f: Pyrite permineralization. c: Ventral view, V. 22963(1) from Sheppey, originally listed as Mastixia cantiensis. d: Lateral view, V. 22969 from Sheppey (identified as Mastixia grandis by Reid and Chandler 1933: pl. 25, fig. 8). e: Equatorial transverse physical section from (c). f: Equatorial transverse physical section from (d). g: Detail of pericarp from (e), showing endocarp formed of dense fibrous tissue, surrounded by mesocarp of anticlinally oriented larger cells. h: Detail of pericarp from (f). i–n: Type material from Eocene of Riestadt, Germany, MNB. i: Ventral view. j, k: Ventral and apical views of holotype. l: View of the transversely fractured surface from (j) showing horseshoe shaped locule. m: Equatorial transverse physical cut of the specimen in (i); note yellow resin cavity (arrow). n: Scanning electron microscopy of pericarp from (l) with locule lining at lower edge of image. Note dense endocarp tissue composed of small cells (fibres and sclereids), extending about 3/5 of distance to periphery, surrounded by mesocarp of larger, anticlinally oriented cells. Scale bars 1 cm in (a–f), (i–k), 1 mm in (g), 2 mm in (h), 3 mm in (l), m, 250 Μm in (n). Bar in (d) applies also to (c). Bar in (l) also applies to (m). Bar in (i) also applies to (j) and (k). in Mastixioid Fruits (Cornales) From The Early Eocene London Clay Flora: Morphology, Anatomy And Nomenclatural Revision

Text-fig. 5. Mastixiopsis nyssoides KIRCHH. a, b, g–n: Organic preservation. a, b: Lignitic, unpermineralized, early Eocene Dorset Pipe clays at Arne, V. 40762. a: Ventral view (original illustration from pl. 18, fig. 1 of Chandler 1962). b: Transverse fracture, somewhat distorted by compression. c–f: Pyrite permineralization. c: Ventral view, V. 22963(1) from Sheppey, originally listed as Mastixia cantiensis. d: Lateral view, V. 22969 from Sheppey (identified as Mastixia grandis by Reid and Chandler 1933: pl. 25, fig. 8). e: Equatorial transverse physical section from (c). f: Equatorial transverse physical section from (d). g: Detail of pericarp from (e), showing endocarp formed of dense fibrous tissue, surrounded by mesocarp of anticlinally oriented larger cells. h: Detail of pericarp from (f). i–n: Type material from Eocene of Riestadt, Germany, MNB. i: Ventral view. j, k: Ventral and apical views of holotype. l: View of the transversely fractured surface from (j) showing horseshoe shaped locule. m: Equatorial transverse physical cut of the specimen in (i); note yellow resin cavity (arrow). n: Scanning electron microscopy of pericarp from (l) with locule lining at lower edge of image. Note dense endocarp tissue composed of small cells (fibres and sclereids), extending about 3/5 of distance to periphery, surrounded by mesocarp of larger, anticlinally oriented cells. Scale bars 1 cm in (a–f), (i–k), 1 mm in (g), 2 mm in (h), 3 mm in (l), m, 250 Μm in (n). Bar in (d) applies also to (c). Bar in (l) also applies to (m). Bar in (i) also applies to (j) and (k).

Text-fig. 1. Diplopanax cacaoides (ZENKER) comb. nov. a–d: [Holotype of Mastixia cantia E.REID et M.CHANDLER, V.22953]. a: Lateral view of longitudinally broken specimen, reflected light. b–d: Surface renderings from micro-CT data. b: Lateral view of longitudinal fracture surface. c: Same specimen rotated to show external surface. d: Enlargement of lower half from (a, b), reflected light. e, f: Specimen figured originally as a paratype of M. cantia, V.22954 (Reid and Chandler 1933: pl. 25, fig. 3), reflected light. e: Ventral view with much of the endocarp wall fallen away exposing smooth convex ventral surface of locule cast. f: Transversely fractured surface, showing thick wall of the endocarp, and dehiscence plane leading to the left limb of the locule. g: Transversely sectioned, laterally compressed specimen from Miocene of Wiesa, Germany for comparison, Senckenberg Museum, SM.B. 21034/I. h–j: Digital transverse sections from micro-CT data of the Holotype V.22953. h: Transverse fracture surface from (b), showing curved locule and zone of weakness defining the germination valve (arrow), reflected light. i: Same orientation with clear demarcation of the separation plane of the germination valve (arrow), digital section from micro-CT scan. j: Enlargement from (h). Scale bars 5 mm. in Mastixioid Fruits (Cornales) From The Early Eocene London Clay Flora: Morphology, Anatomy And Nomenclatural Revision

Text-fig. 1. Diplopanax cacaoides (ZENKER) comb. nov. a–d: [Holotype of Mastixia cantia E.REID et M.CHANDLER, V.22953]. a: Lateral view of longitudinally broken specimen, reflected light. b–d: Surface renderings from micro-CT data. b: Lateral view of longitudinal fracture surface. c: Same specimen rotated to show external surface. d: Enlargement of lower half from (a, b), reflected light. e, f: Specimen figured originally as a paratype of M. cantia, V.22954 (Reid and Chandler 1933: pl. 25, fig. 3), reflected light. e: Ventral view with much of the endocarp wall fallen away exposing smooth convex ventral surface of locule cast. f: Transversely fractured surface, showing thick wall of the endocarp, and dehiscence plane leading to the left limb of the locule. g: Transversely sectioned, laterally compressed specimen from Miocene of Wiesa, Germany for comparison, Senckenberg Museum, SM.B. 21034/I. h–j: Digital transverse sections from micro-CT data of the Holotype V.22953. h: Transverse fracture surface from (b), showing curved locule and zone of weakness defining the germination valve (arrow), reflected light. i: Same orientation with clear demarcation of the separation plane of the germination valve (arrow), digital section from micro-CT scan. j: Enlargement from (h). Scale bars 5 mm.

Text-fig. 4. Mastixia cf. oregonensis (R.A.SCOTT) TIFFNEY et HAGGARD from the London Clay, originally included within the concept of M. cantiensis. a–c: V. 22960(1). a: Transverse fracture, showing c-shaped locule, dorsal infold, and sculptured endocarp, reflected light. b: Transverse digital section from micro-CT scan data. c: Surface view of ribbed endocarp extracted from micro-CT data. d: Transverse fracture, reflected light, V. 22955 (originally illustrated in pl. 25, fig. 4 of Reid and Chandler 1933). e, f: Transverse physical section, V. 22963(2) showing U-shaped locule and longitudinal dorsal infold. g–i reflected light. g: Detail from left of (d). h, i: Detail from right of (f). Scale bars 5 mm in (a–f), 1 mm in (g–i). in Mastixioid Fruits (Cornales) From The Early Eocene London Clay Flora: Morphology, Anatomy And Nomenclatural Revision

Text-fig. 4. Mastixia cf. oregonensis (R.A.SCOTT) TIFFNEY et HAGGARD from the London Clay, originally included within the concept of M. cantiensis. a–c: V. 22960(1). a: Transverse fracture, showing c-shaped locule, dorsal infold, and sculptured endocarp, reflected light. b: Transverse digital section from micro-CT scan data. c: Surface view of ribbed endocarp extracted from micro-CT data. d: Transverse fracture, reflected light, V. 22955 (originally illustrated in pl. 25, fig. 4 of Reid and Chandler 1933). e, f: Transverse physical section, V. 22963(2) showing U-shaped locule and longitudinal dorsal infold. g–i reflected light. g: Detail from left of (d). h, i: Detail from right of (f). Scale bars 5 mm in (a–f), 1 mm in (g–i).

Text-fig. 3. Juglandaceae. Carya (a–x). Scale bars = 1 cm. a–e: USNM PAL 772346. Micro-CT scan surface rendering. a, b: Lateral, c: apical, d: basal views. e: Virtual equatorial transverse section. f–n: USNM PAL 772347. f: Lateral view, reflected light, showing path of saw cut for transverse section of (i). g: Basal view, reflected light. h: Apical view, micro-CT surface rendering. i: Physical transverse section displaying locule and cellular preservation of parts of wall. j–n: Virtual sections from micro-CT scan data. j: Transverse section at apical 1/3 of nut. Note narrow lacunae (arrows). k: Longitudinal section parallel to primary septum, traversing one of the cotyledon lobes and showing secondary septum at base. l: Longitudinal section in plane at right angles to (k) in plane of primary septum, showing divergent placental bundles arising from base of nut (arrows). m: Equatorial transverse section showing two lobes of locule separated by primary septum. n: Transverse section near base of nut showing primary and secondary septa, creating four basal lobes of locule; note diverging placental bundles (arrows). o–x: USNM PAL 772351. o: Lateral view of broken nut with exposed locule cast, reflected light. p: Same orientation of nut, micro-CT surface rendering. q: Same specimen lateral view, rotated 90° from (p), micro-CT surface rendering. r: Apical view, reflected light. s–x: Virtual sections from micro-CT in The Early Middle Eocene Wagon Bed Carpoflora Of Central Wyoming, U.S.A.

Text-fig. 3. Juglandaceae. Carya (a–x). Scale bars = 1 cm. a–e: USNM PAL 772346. Micro-CT scan surface rendering. a, b: Lateral, c: apical, d: basal views. e: Virtual equatorial transverse section. f–n: USNM PAL 772347. f: Lateral view, reflected light, showing path of saw cut for transverse section of (i). g: Basal view, reflected light. h: Apical view, micro-CT surface rendering. i: Physical transverse section displaying locule and cellular preservation of parts of wall. j–n: Virtual sections from micro-CT scan data. j: Transverse section at apical 1/3 of nut. Note narrow lacunae (arrows). k: Longitudinal section parallel to primary septum, traversing one of the cotyledon lobes and showing secondary septum at base. l: Longitudinal section in plane at right angles to (k) in plane of primary septum, showing divergent placental bundles arising from base of nut (arrows). m: Equatorial transverse section showing two lobes of locule separated by primary septum. n: Transverse section near base of nut showing primary and secondary septa, creating four basal lobes of locule; note diverging placental bundles (arrows). o–x: USNM PAL 772351. o: Lateral view of broken nut with exposed locule cast, reflected light. p: Same orientation of nut, micro-CT surface rendering. q: Same specimen lateral view, rotated 90° from (p), micro-CT surface rendering. r: Apical view, reflected light. s–x: Virtual sections from micro-CT

Text-fig. 7. Cornacaeae (a–j), Icacinaceae (k–o). a–e: Mastixia. USNM PAL 772364. Scale bar = 1 cm. a: Lateral view of eroded endocarp – the opposite side being missing and the endocarp broken near its mid point, reflected light, palladium coated. b–e: Micro-CT scan surface renderings. b: Rotated 90° from the view in (a). c: Rotated 90° from the view in (b). d: Rotated 90° from (c), exhibiting the damaged "back" face of the endocarp. e: Axillary view of the endocarp; the opposite end missing as apparent in (d). f–j: Cf. Nyssa. DMNH EPI.47808. Scale bar = 1 cm. Micro-CT scan surface renderings. f: Intact face of the endocarp; note ridges and "apical" point. g: Eroded (?gnawed; note horizontal grooving) opposite face of the endocarp. h: Lateral view of the endocarp, eroded/gnawed face to left. i: Apical view, eroded/gnawn portion below. j: Basal view of endocarp. k–o: Iodes DMNH-EPI.47807. Micro-CT scan surface renderings. Scale bar = 1 cm. k: Face view of endocarp. l: Opposite face of endocarp. m: Lateral view demonstrating the compressed nature of the endocarp, note thickened suture marking the probable track of the primary bundle. n: Apical view, primary bundle trace to right. o: Basal view, primary bundle trace to right. in The Early Middle Eocene Wagon Bed Carpoflora Of Central Wyoming, U.S.A.

Text-fig. 7. Cornacaeae (a–j), Icacinaceae (k–o). a–e: Mastixia. USNM PAL 772364. Scale bar = 1 cm. a: Lateral view of eroded endocarp – the opposite side being missing and the endocarp broken near its mid point, reflected light, palladium coated. b–e: Micro-CT scan surface renderings. b: Rotated 90° from the view in (a). c: Rotated 90° from the view in (b). d: Rotated 90° from (c), exhibiting the damaged "back" face of the endocarp. e: Axillary view of the endocarp; the opposite end missing as apparent in (d). f–j: Cf. Nyssa. DMNH EPI.47808. Scale bar = 1 cm. Micro-CT scan surface renderings. f: Intact face of the endocarp; note ridges and "apical" point. g: Eroded (?gnawed; note horizontal grooving) opposite face of the endocarp. h: Lateral view of the endocarp, eroded/gnawed face to left. i: Apical view, eroded/gnawn portion below. j: Basal view of endocarp. k–o: Iodes DMNH-EPI.47807. Micro-CT scan surface renderings. Scale bar = 1 cm. k: Face view of endocarp. l: Opposite face of endocarp. m: Lateral view demonstrating the compressed nature of the endocarp, note thickened suture marking the probable track of the primary bundle. n: Apical view, primary bundle trace to right. o: Basal view, primary bundle trace to right.

Text-fig. 10. Carpolithes (a–j). a–d: Carpolithes sp. 10. USNM PAL 772375. Scale bar = 5 mm, reflected light, palladium coated. a: Lateral view of one face of structure; note adherent mineral material. Longitudinal groove is to right. b: Lateral view of one edge of the structure. c: Opposite view from (b), note groove in upper half of the specimen, facing viewer. d: Apical view. e–j: Carpolithes sp. 11. USNM PAL 772376. Scale bar = 5 mm, micro-CT scan surface views. e: Structure in face view showing central protuberance. f: Same, lateral view. g: Opposite face from (e). h: Opposite face from (f). i: View from one end. j: View from opposite end from (i). in The Early Middle Eocene Wagon Bed Carpoflora Of Central Wyoming, U.S.A.

Text-fig. 10. Carpolithes (a–j). a–d: Carpolithes sp. 10. USNM PAL 772375. Scale bar = 5 mm, reflected light, palladium coated. a: Lateral view of one face of structure; note adherent mineral material. Longitudinal groove is to right. b: Lateral view of one edge of the structure. c: Opposite view from (b), note groove in upper half of the specimen, facing viewer. d: Apical view. e–j: Carpolithes sp. 11. USNM PAL 772376. Scale bar = 5 mm, micro-CT scan surface views. e: Structure in face view showing central protuberance. f: Same, lateral view. g: Opposite face from (e). h: Opposite face from (f). i: View from one end. j: View from opposite end from (i).

Text-fig. 6. Cornaceae. Alangium (a–e), Mastixia (f–r). a–e: Alangium, DMNH EPI.47806. Scale bar = 1 cm. b, e: Reflected light, palladium coated. a, c, d: Micro-CT scan surface rendering. a: Locule cast, face view of slightly larger locule. b: Face view of slightly smaller locule. c: Lateral view of the endocarp, the slightly enlarged left carpel separated from the smaller carpel by a longitudinal septal groove; the faint pitting in the groove suggestive of the septal vasculature. d, e: Views of either end of the endocarp, illustrating the size difference between the two carpels and the pitting in the septal groove suggestive of the septal vasculature. f–k: Mastixia USNM PAL 772362. Scale bar = 1 cm. f, g, j, k: reflected light, palladium coated; h, i: micro-CT scan surface rendering. f: Lateral view of endocarp, inferred dorsal germination valve groove facing the viewer. Note irregular, rugose, longitudinal ridges. g: Lateral view of endocarp, inferred germination valve with median longitudinal groove to left. h: Lateral view of endocarp reoriented with the same longitudinal groove to the right. i: Lateral view, rotated to ventral surface. j: View of one end of the endocarp, germination valve groove up. k: Opposite end view, with prominent radial ridges and intervening grooves, germination valve groove up. l–r: Mastixia USNM PAL 772363. Scale bar = 1 cm. l: View of one face of endocarp, displaying a groove that may represent the surficial expression of the dorsal infold of a Mastixia-like germination valve. Surface badly eroded, reflected light, palladium coated. m: Opposite face of endocarp displaying extensive erosion and a central hole interpreted as feeding damage. n: Lateral view; m, n micro-CT scan surface renderings. o: A view of one end, displaying the prominent groove, reflected light, palladium coated. p: Opposite end to (o). q: View as in (o); p, q micro-CT scan surface renderings. r: Virtual transverse section showing curved locule (arrows). in The Early Middle Eocene Wagon Bed Carpoflora Of Central Wyoming, U.S.A.

Text-fig. 6. Cornaceae. Alangium (a–e), Mastixia (f–r). a–e: Alangium, DMNH EPI.47806. Scale bar = 1 cm. b, e: Reflected light, palladium coated. a, c, d: Micro-CT scan surface rendering. a: Locule cast, face view of slightly larger locule. b: Face view of slightly smaller locule. c: Lateral view of the endocarp, the slightly enlarged left carpel separated from the smaller carpel by a longitudinal septal groove; the faint pitting in the groove suggestive of the septal vasculature. d, e: Views of either end of the endocarp, illustrating the size difference between the two carpels and the pitting in the septal groove suggestive of the septal vasculature. f–k: Mastixia USNM PAL 772362. Scale bar = 1 cm. f, g, j, k: reflected light, palladium coated; h, i: micro-CT scan surface rendering. f: Lateral view of endocarp, inferred dorsal germination valve groove facing the viewer. Note irregular, rugose, longitudinal ridges. g: Lateral view of endocarp, inferred germination valve with median longitudinal groove to left. h: Lateral view of endocarp reoriented with the same longitudinal groove to the right. i: Lateral view, rotated to ventral surface. j: View of one end of the endocarp, germination valve groove up. k: Opposite end view, with prominent radial ridges and intervening grooves, germination valve groove up. l–r: Mastixia USNM PAL 772363. Scale bar = 1 cm. l: View of one face of endocarp, displaying a groove that may represent the surficial expression of the dorsal infold of a Mastixia-like germination valve. Surface badly eroded, reflected light, palladium coated. m: Opposite face of endocarp displaying extensive erosion and a central hole interpreted as feeding damage. n: Lateral view; m, n micro-CT scan surface renderings. o: A view of one end, displaying the prominent groove, reflected light, palladium coated. p: Opposite end to (o). q: View as in (o); p, q micro-CT scan surface renderings. r: Virtual transverse section showing curved locule (arrows).

Text-fig. 8. Carpolithes (a–t). a–e: Carpolithes sp. 1. USNM PAL 772366. Scale bar = 1 cm. a: Lateral view of endocarp, note two longitudinal ridges. b: Lateral view of endocarp rotated 90° from (a), note single lateral ridge in center, a, b reflected light, palladium coated. c: Lateral view, Micro-CT scan surface rendering. d: View of rounded end of the endocarp, reflected light, palladium coated. e: View of the opposite (pointed) end of the endocarp, note split; reflected light, palladium coated. f–j: Carpolithes sp. 2. USNM PAL 772367. Scale bar = 5 mm. f: Lateral view, base down; note raphe-like structure (arrow), reflected light, palladium coated. g: Lateral view, the raphe-like structure extending vertically from the base. h: Lateral view, rotated 90° from (g). i: Lateral view, the opposite face to that in (h). j: Basal view, raphe-like structure running from the center to the right of the image. g–j: CT scan surface renderings. k–o: Carpolithes sp. 3 USNM PAL 772368. Scale bar = 5 mm. k: Ventral view of the specimen, note flared apical extension, reflected light, uncoated. l: Dorsal view illustrating the flared apical extension, rotated 180o from (k). m: Lateral view rotated 90° from that in (l). n: Apical view, the apical extension with central pore (arrow) and a clear lineation running down the side to the top of the image. o: Basal view. l–o: Micro-CT scan surface renderings. p–t: Carpolithes sp. 4. USNM PAL 772369. Scale bar = 3 mm. p: Basal view illustrating the concentric rings of radiating possible cells surrounding a central depression. q: Lateral view, base down, note possible cellular pattern. r: Lateral view, rotated 180° from (q), base down; p–r: reflected light, palladium coated. s, t: Basal and lateral views, micro-CT scan surface renderings. in The Early Middle Eocene Wagon Bed Carpoflora Of Central Wyoming, U.S.A.

Text-fig. 8. Carpolithes (a–t). a–e: Carpolithes sp. 1. USNM PAL 772366. Scale bar = 1 cm. a: Lateral view of endocarp, note two longitudinal ridges. b: Lateral view of endocarp rotated 90° from (a), note single lateral ridge in center, a, b reflected light, palladium coated. c: Lateral view, Micro-CT scan surface rendering. d: View of rounded end of the endocarp, reflected light, palladium coated. e: View of the opposite (pointed) end of the endocarp, note split; reflected light, palladium coated. f–j: Carpolithes sp. 2. USNM PAL 772367. Scale bar = 5 mm. f: Lateral view, base down; note raphe-like structure (arrow), reflected light, palladium coated. g: Lateral view, the raphe-like structure extending vertically from the base. h: Lateral view, rotated 90° from (g). i: Lateral view, the opposite face to that in (h). j: Basal view, raphe-like structure running from the center to the right of the image. g–j: CT scan surface renderings. k–o: Carpolithes sp. 3 USNM PAL 772368. Scale bar = 5 mm. k: Ventral view of the specimen, note flared apical extension, reflected light, uncoated. l: Dorsal view illustrating the flared apical extension, rotated 180o from (k). m: Lateral view rotated 90° from that in (l). n: Apical view, the apical extension with central pore (arrow) and a clear lineation running down the side to the top of the image. o: Basal view. l–o: Micro-CT scan surface renderings. p–t: Carpolithes sp. 4. USNM PAL 772369. Scale bar = 3 mm. p: Basal view illustrating the concentric rings of radiating possible cells surrounding a central depression. q: Lateral view, base down, note possible cellular pattern. r: Lateral view, rotated 180° from (q), base down; p–r: reflected light, palladium coated. s, t: Basal and lateral views, micro-CT scan surface renderings.